Stability of Plasmid DNA during Liposome Encapsulation

Liposome 봉입과정에서의 Plasmid DNA의 안정성

  • Ahn, J.S. (Department of Biological Science & Engineering, Korea Advanced Institute of Science and Technology) ;
  • Pack, M.Y. (Department of Biological Science & Engineering, Korea Advanced Institute of Science and Technology)
  • Published : 1985.09.01

Abstract

Plasmids, YEp13 and pMA56, were encapsulated Into liposomes by two different procedures during which the plasmid DNAs were exposed ether to 6$0^{\circ}C$ for 1.5 hr or to sonication for 2-5 min at 4$^{\circ}C$. The encapsulated plasmids were then reextracted and their physical conformations and transformation abilities were examined. It was confirmed from the results that both plasmid DNAs were remained stable throughout the procedures of encapsulation into 1iposomes.

플라스미드 YEp13과 pMA56을 두가지 다른 방법으로 liposome속에 봉입시켰다. 이 두가지 방법에 따르면 플라스미드 DNA는 6$0^{\circ}C$에 1.5시간 동안 노출되거나, 4$^{\circ}C$에서 2-5분 동안 sonication 처리를 받아야 한다. 일단 봉입된 플라스미드를 다시 뽑아낸 다음, 그들에 대한 물리적인 구조와 유전적 전환능력을 조사했다. 그 결과 이 두 플라스미드 DNA는 liposome 봉입과정을 통해 아무런 손상을 입지 않았음이 밝혀졌다.

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