• 한국자원식물학회지
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• 제24권2호
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• pp.181-188
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• 2011

본 연구는 회향종자 물 추출물이 비만과 관련된 지질대사를 조절하는 효소들 가운데 lipoprotein lipase(LPL), acyl-CoA synthetase(ACS), hormone sensitive lipase(HSL)의 활성에 미치는 효과를 알아보았다. 정상 마우스에서 회향종자 물 추출물의 LPL 활성은 최고 21.9% 억제되었고, ACS와 HSL의 효소 활성은 각각 151.8%, 174.0% 증가되었다. 고지방식이 유도 비만 마우스에서 LPL은 12.0% 활성이 억제되었으며, ACS와 HSL은 742.0%, 134.4% 활성이 증가하였다. 결과적으로 회향종자 물 추출물이 효과적으로 세포내로 지방산의 유입을 억제하며, 유입된 지방산을 에너지원으로 사용하는 대사과정을 활성화시킴으로서 항비만 효능을 갖는다는 것을 추정할 수 있다.

• 생약학회지
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• 제43권3호
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• pp.201-205
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• 2012

In the course of screening pancreatic lipase inhibitory activity, total methanolic extract and EtOAc-soluble fraction of Duchesnea chrysantha showed significant inhibitory activity. Further fractionation and isolation of the EtOAc-soluble fraction resulted in five compounds, which were identified as trans-tiliroside (1), isovitexin (2), kaempferol-8-O-${\beta}$-glucoside (3), kaempferol-3-O-${\beta}$-glucoside (4) and quercetin-3-O-${\beta}$-glucoside (5). All the five flavonoids derivatives were first reported from this plant but showed weak inhibitory effects on pancreatic lipase activity.

• Applied Biological Chemistry
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• 제14권3호
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• pp.207-212
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• 1971

An enzyme preparation from a newly isolated Candida that showed a high lipase activity was subjected to examination of its reaction rate under various conditions. The original and a diluted enzyme solutions showed the zero order curve starting at the point of 50 minutes in time. When PVA was used as an emulsifyer more activity was observed than the case of gum arabic. The optimal temperature and pH were $37{\sim}40^{\circ}C$ and 7.0, respectively. Oleic acid as a fatty acid conferred on the enzyme an inhibitory action while calcium ion a positive one. Sodium cholate yielded an increase in reaction rate at the first stage.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 1986년도 추계학술대회
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• pp.523.2-523
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• 1986

The hydrolysis of olive oil was attempted with immobilized C. rugosa lipase in the reverse phase solvent system. (i.e. immobilized wet particles is dispersed in continuous phase olive oil or organic solvents containing olive oil). Sephadex LH-20 and LH-60 were used as the supports that can be used in organic solvents. The water content of wet particles of sephadex LH-20 and LH-60 were about 72% (w/w) and 85% (w/w), respectively Both swollen gels with 0.05M buffers adsorbed about 18% of lipase dissolved. They were easily dispersed in liquid olive oil or in organic solvents. The effects of organic solvents on the stability and catalytic activity of the lipase have been examined. The results revealed that isooctane is superior to the other solvents examined for enzymatic fat spliting in reverse phase system. Kinetics of enzymatic hydrolys of olive oil by immobilized lipase has been investigated in a batch reactor. Effects of pH and temperature on the lipase were studied. The substrate concentration was influenced positively on the thermal stability.

• Journal of Applied Biological Chemistry
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• 제48권2호
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• pp.84-88
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• 2005

To develop functional food and anti-obesity drug through inhibition of dietary lipid absorption, inhibitory effects of herb extracts on pancreatic lipase were investigated. Due to high yield and simplicity of isolation, lipase inhibitor (ELI) was isolated from ethyl acetate extract of Eugenia aromaticum, which showed highest inhibitory activity, and characterized for development of novel functional material. Stability of ELI at high temperature and low pH was investigated. Results showed ELI is relatively stable under thermal and acidic conditions, reversible, and noncompetitive inhibitor of pancreatic lipase.

• Bulletin of the Korean Chemical Society
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• 제35권2호
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• pp.477-482
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• 2014

We immobilized Thermomyces lanuginosus lipase to catalyze transesterification reaction in DMF. This lipase was selected after screening among other commercial lipases. We found that prepared immobilized lipase is particularly useful for preparation of 6-O-acylsucrose with higher conversion rate even in 10 g scale. Several solvents were evaluated for selective transesterification reaction. We noticed that the immobilized lipase retained more than 80 % activity after 5 cycles of 96 h reaction. A general method was also developed to purify the products using simple crystallization and precipitation process. Furthermore, 6-O-vinyladipoylsucrose was subjected to synthesis of the corresponding polymer by radical initiator. The sucrose branched polymer can be used further for evaluation of its biodegradability and other biological applications.

• Asian-Australasian Journal of Animal Sciences
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• 제31권4호
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• pp.569-575
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• 2018

Objective: This experiment was conducted to investigate the effects of chromium picolinate (CrP) on fat deposition, genetic expression and enzymatic activity of lipid metabolism-related enzymes. Methods: Two hundred forty one-day-old Ross broilers were randomly divided into 5 groups with 4 replicates per group and 12 Ross broiler chicks per replicate. The normal control group was fed a basal diet, and the other groups fed the same basal diet supplemented with 0.1, 0.2, 0.4, and 0.8 mg/kg CrP respectively. The experiment lasted for 21 days. Results: Added CrP in the basal diet decreased the abdominal fat, had no effects on subcutaneous fat thickness and inter-muscular fat width; 0.2 mg/kg CrP significantly decreased the fatty acid synthase (FAS) enzymatic (p<0.05); acetyl-CoA carboxylase (ACC) enzymatic activity decreased in all CrP groups (p<0.05); hormone-sensitive lipase (HSL) enzymatic activity also decreased, but the change was not significant (p>0.05); 0.4 mg/kg CrP group significantly decreased the lipoprotein lipase (LPL) enzymatic activity. FAS mRNA expression increased in all experimental groups, and the LPL mRNA expression significantly increased in all experimental groups (p<0.05), but not 0.2 mg/kg CrP group. Conclusion: The results indicated that adding CrP in basal diet decreased the abdominal fat percentage, had no effects on subcutaneous fat thickness and inter-muscular fat width, decreased the enzymatic activity of FAS, ACC, LPL and HSL and increased the genetic expression levels of FAS and LPL.

• 분석과학
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• 제30권3호
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• pp.138-145
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• 2017

Microwave-assisted organic synthesis has gained a remarkable interest over the past years because of its advantages - (i) rapid energy transfer and superheating, (ii) higher yield and rapid reaction, (iii) cleaner reactions. Ionic liquids are well known for their unique properties such as negligible vapor pressure and high thermal stability. With these properties, ionic liquids have gained increasing attention as green, multi-use reaction media. Recently, ionic liquids have been applied as reaction media for biocatalysis. Lipase-catalyzed reactions in ionic liquids provide high activity and yield compared to conventional organic solvents or solvent free system. Since polar molecules are generally good absorbent to microwave radiation, ionic liquids were investigated as reaction media to improve activity and productivity. In this study, therefore, the effect of microwave irradiation in ionic liquids was investigated on lipase catalyzed reactions such as benzyl acetate synthesis and caffeic acid phenethyl ester synthesis. Comparing to conventional heating, microwave heating showed almost the same final conversion but increased initial reaction rate (3.03 mM/min) compared to 2.11 mM/min in conventional heating at $50^{\circ}C$.

• 한국미생물·생명공학회지
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• 제47권4호
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• pp.546-554
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• 2019

This study, for the first time, reports the functional expression of lipase B derived from the yeast Candida antarctica (CALB) in Corynebacterium strain using the Escherichia coli plasmid PK18. The CALB gene fragment encoding a 317-amino-acid protein was successfully obtained from the total RNA of C. antarctica. CALB was readily produced in the Corynebacterium strain without the use of induction methods described in previous studies. This demonstrated the extracellular production of CALB in the Corynebacterium strain. CALB produced in the Corynebacterium MB001 strain transformed with pEC-CALB recombinant plasmid exhibited maximum extracellular enzymatic activity and high substrate affinity. The optimal pH and temperature for the hydrolysis of 4-nitrophenyl laurate by CALB were 9.0 and 40℃, respectively. The enzyme was stable at pH 10.7 in the glycine-KOH buffer and functioned as an alkaline lipase. The CALB activity was inhibited in the presence of high concentration of Mg²⁺, which indicated that CALB is not a metalloenzyme. These properties are key for the industrial application of the enzyme.

• 융합정보논문지
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• 제12권3호
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• pp.126-131
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• 2022

비만은 에너지의 섭취와 소비의 불균형으로 지방조직이 비정상적으로 분화하면서 생기는 대사질환으로 알려져있다. 본 연구에서는 본 연구는 적양파 추출물 처리에 따른 Pancreatic lipase 억제, 지방세포분화 억제 활성을 확인하고자 하였다. 적양파추출물 처리에 따른 활성은 지방세포 분화 및 관련 유전자에 대한 평가는 3T3-L1 지방전구세포를 이용하고 Real-Time PCR을 통하여 확인하였다. 실험 결과, Pancreatic lipase 활성 억제 실험에서 적양파 추출물은 농도 의존적으로 lipase 활성을 억제하였다. 지방세포분화 실험을 수행한 결과, insulin, dexamethasone, 3-isobutyl-1-methylxanthine(MDI)등으로 분화 유도된 3T3-L1 세포에서 적양파 추출물은 지방전구세포의 분화를 억제하였으며 동시에 지방구 형성을 억제하는 것으로 나타났다. 또한, 지방전구세포의 분화 과정과 관련된 C/EBP-α, C/EBP-β, PPAR-γ의 발현을 억제하였다. 본 실험에서 적양파 추출물은 지방분해효소를 억제하며, 지방전구세포 분화와 관련된 유전자 발현을 억제함으로써 지방세포 분화 및 지방구형성을 억제할 수 있는 항비만 소재로의 개발 가능성이 높다고 판단된다.