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http://dx.doi.org/10.5713/ajas.17.0289

Effects of chromium picolinate on fat deposition, activity and genetic expression of lipid metabolism-related enzymes in 21 day old Ross broilers  

Chen, Guangxin (State Key Laboratory of Special Animal Molecular Biology)
Gao, Zhenhua (College of Agriculture, Guangdong Ocean University)
Chu, Wenhui (State Key Laboratory of Special Animal Molecular Biology)
Cao, Zan (College of Agriculture, Guangdong Ocean University)
Li, Chunyi (State Key Laboratory of Special Animal Molecular Biology)
Zhao, Haiping (State Key Laboratory of Special Animal Molecular Biology)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.31, no.4, 2018 , pp. 569-575 More about this Journal
Abstract
Objective: This experiment was conducted to investigate the effects of chromium picolinate (CrP) on fat deposition, genetic expression and enzymatic activity of lipid metabolism-related enzymes. Methods: Two hundred forty one-day-old Ross broilers were randomly divided into 5 groups with 4 replicates per group and 12 Ross broiler chicks per replicate. The normal control group was fed a basal diet, and the other groups fed the same basal diet supplemented with 0.1, 0.2, 0.4, and 0.8 mg/kg CrP respectively. The experiment lasted for 21 days. Results: Added CrP in the basal diet decreased the abdominal fat, had no effects on subcutaneous fat thickness and inter-muscular fat width; 0.2 mg/kg CrP significantly decreased the fatty acid synthase (FAS) enzymatic (p<0.05); acetyl-CoA carboxylase (ACC) enzymatic activity decreased in all CrP groups (p<0.05); hormone-sensitive lipase (HSL) enzymatic activity also decreased, but the change was not significant (p>0.05); 0.4 mg/kg CrP group significantly decreased the lipoprotein lipase (LPL) enzymatic activity. FAS mRNA expression increased in all experimental groups, and the LPL mRNA expression significantly increased in all experimental groups (p<0.05), but not 0.2 mg/kg CrP group. Conclusion: The results indicated that adding CrP in basal diet decreased the abdominal fat percentage, had no effects on subcutaneous fat thickness and inter-muscular fat width, decreased the enzymatic activity of FAS, ACC, LPL and HSL and increased the genetic expression levels of FAS and LPL.
Keywords
Ross Broiler; Chromium Picolinate (CrP); Fatty Acid Synthase; Acetyl-CoA Carboxylase; Hormone-sensitive Lipase; Lipoprotein Lipase; Enzymatic Activity; mRNA Expression;
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