• BMB Reports
• /
• v.39 no.4
• /
• pp.426-431
• /
• 2006

Embryonic stem cells (ESCs), representing a population of undifferentiated pluripotent cells with both self-renewal and multilineage differentiation characteristics, are capable of spontaneous differentiation into cardiomyocytes. The present study sought to define the kinetic characterization of lactate dehydrogenase (LDH) and creatine kinase (CK) of ESC- and neonatal-derived cardiomyocytes. Spontaneously differentiated cardiomyocytes from embryoid bodies (EBs) derived from mouse ESC line (Royan B1) and neonatal cardiomyocytes were dispersed in a buffer solution. Enzymes were extracted by sonication and centrifugation for kinetic evaluation of LDH and CK with spectrophotometric methods. While a comparison between the kinetic properties of the LDH and CK of both groups revealed not only different Michaelis constants and optimum temperatures for LDH but also different Michaelis constants and optimum pH for CK, the pH profile of LDH and optimum temperature of CK were similar. In defining some kinetic properties of cardiac metabolic enzymes of ESC-derived cardiomyocytes, our results are expected to further facilitate the use of ESCs as an experimental model.

• The Transactions of the Korean Institute of Electrical Engineers D
• /
• v.50 no.1
• /
• pp.14-21
• /
• 2001

This paper proposes an indirect adaptive fuzzy controller for general SISO nonlinear systems. In indirect adaptive fuzzy control, based on the proved approximation capability of fuzzy systems, they are used to capture the unknown nonlinearities of the plant. Until now, most of the papers in the field of controller design for nonlinear system considers the affine system using fuzzy systems which have fixed grid-rule structure. We proposes a dynamic fuzzy rule insertion scheme where fuzzy rule-base grows as time goes on. With this method, the dynamic order of the controller reduces dramatically and an appropriate number of fuzzy rules are found on-line. No a priori information on bounding constants of uncertainties including reconstruction errors and optimal fuzzy parameters is needed. The control law and the update laws for fuzzy rule structure and estimates of fuzzy parameters and bounding constants are determined so that the Lyapunov stability of the whole closed-loop system is guaranteed.

• The Transactions of the Korean Institute of Power Electronics
• /
• v.14 no.6
• /
• pp.481-487
• /
• 2009

This paper proposes a regulation method of DC line voltage for urban transit system fluctuated during the acceleration or deceleration by using supercapacitor. Supercapacitor is modelled electrically under the assumption of three different time constants of RC circuits with variable capacitances depending on the voltage. And its parameters are determined by the experimental measurements. The energy storage system using supercapacitors is installed based on this model, and the proposed model is tested through the simulations and experiments, and the controller for charging and discharging is designed. Finally, it is tested at Kyoungsan test site for the urban light rail road system and the energy saving effect is evaluated economically.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.29 no.9A
• /
• pp.983-990
• /
• 2004

In this paper, we calculated the input impedance of the power line communication(PLC) networks using medium voltage power line. First of all, we proposed input and output teoninal model of PLC network, and calculated the input impedance applying the attenuation constants by radiation loss, conductor loss, dielectric loss. From the calculation result, we knew that the attenuation by radiation loss was largest, and the input impedance appears like a standing wave fonn with a fixed cycle because the high reflection at the input terminal for the characteristic impedance of the power line is very large. And also the cycle of input impedance depends on the coaxial cable length, and the amplitude depends on the characteristic impedance of power line and losses. From the measurement result, calculated result was very similar to the measured result.

• The Transactions of The Korean Institute of Electrical Engineers
• /
• v.64 no.6
• /
• pp.857-863
• /
• 2015

OF cable with excellent reliability and economic efficiency, has been used as the main cable of underground transmission in Korea. However, as XLPE cable is excellent in view points such as reduction of loss, convenience on the construction/operation, and manufacturing technology, it has been replaced as instead of OF cable. Also, when the aging occurs in some sections of OF cable, the section is replaced as XLPE cable without changing of whole cable line. However, there are some differences like electrical constants between both types of cables. Therefore if underground transmission system will be operated as combined connection composited of both cables, it should be proved that overvoltage is stable in steady and transient state. This paper describes the effectiveness of connection operation in combined OF and XLPE cable. For the evaluation of effectiveness, some important analysis such as sheath component voltage, lightning and switching overvoltage, single line-to-ground fault are carried out in steady and transient state. EMTP/ATPDraw is used for modeling and analysis of system.

• Journal of the Korean Ceramic Society
• /
• v.42 no.7 s.278
• /
• pp.449-454
• /
• 2005

A waveguide method is developed to study the materials with relatively large dielectric constants at microwave range. Basically, the method is similar to the previous waveguide methods represented by short-circuit line and transmission/reflection measurement methods. However, the complex permittivity is not determined by the shift in resonance frequencies, but by numerical analysis of measured scattering parameters. In order to enhance microwave penetration into the specimen with relatively large permittivity, a dielectric plate with lower permittivity is employed for impedance matching. The influences of air gap between the specimen and waveguide wall are evaluated, and the corresponding errors are estimated. The propagation of higher order modes is also considered. Experimental results for several reference ceramics are presented.

• Bulletin of the Korean Chemical Society
• /
• v.25 no.5
• /
• pp.609-616
• /
• 2004

Complexation of cesium ion by p-tert-butylcalix[6]arene ethyl ester was studied by NMR spectroscopy in nonpolar $CDCl_3$ and polar acetone-$d_6$ and the results were compared with each other. Analysis of temperature dependent $^1H$ spectra and titration curves reveals that both solvents result in a 1 : 1 cone-form complex with nonpolar $CDCl_3$yielding a more tightly bound one than acetone-$d_6$. Unexpectedly, at very low temperature, we have found that two phenyl ring proton peaks of equal intensity appear both in $CDCl_3$and in acetone-$d_6$ solution which gradually collapse and eventually coalesce into a single line as temperature is raised. This observation could be interpreted in terms of the chemical exchange through direct and/or indirect interconversion between two equivalent conformations possible the complex in both solvents over the temperature range observed. And broadening of $^{133}Cs$ (I = 7/2) nmr line with increasing temperature has also been observed, indicating the exchange of $^{133}Cs$ ion between the complex and the solvent. From numerical fitting of lineshape changes for one-dimensional $^1H$ and $^{133}Cs$ spectra, the exchange rate constants and other relevant parameters for this conformational interconversion and the complex-solvent exchange were deduced.

• Journal of The Korean Astronomical Society
• /
• v.34 no.2
• /
• pp.67-80
• /
• 2001

Chemical evolution of galaxies can be understood by studying the spatial distribution of heavy elements. We selected two nearby galaxies, M31 and M33 and investigated spectrum of their HII regions: a) the elec-tron densities have been derived from the [S II] 6717/6731 ratio along with the most recent atomic constants (Hyung & Aller 1996); b) the electron temperatures were determinated from the Pagel's empirical method. Nebula Model (Hyung 1994) has been employed to predict the spectral line intensities which gives the proper chemical abundances. The model would predict the line intensities correctly only when various input parameters such as the effective central star temperatures, gravity log g, model atmosphere as well as the geometry and the nebula physical condition are appropriate. Thus, the determination of chemical abundances of O, S, N of the two nearby galaxies M31 and M33 has been done, which shows a radial dependance of O/H and N/H: decrease with the distance, or increasing electron temperature due to the elemental deficiency. Abundances of M31 appear to be enhanced than those of M33.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2018.05a
• /
• pp.448-448
• /
• 2018

In this study, the regression models (Load ESTimator and eight-parameter model) were evaluated to estimate instantaneous pollutant loads under various criteria and optimization methods. As shown in the results, LOADEST commonly used in interpolating pollutant loads could not necessarily provide the best results with the automatic selected regression model. It is inferred that the various regression models in LOADEST need to be considered to find the best solution based on the characteristics of watersheds applied. The recently developed eight-parameter model integrated with Genetic Algorithm (GA) and Gradient Descent Method (GDM) were also compared with LOADEST indicating that the eight-parameter model performed better than LOADEST, but it showed different behaviors in calibration and validation. The eight-parameter model with GDM could reproduce the nitrogen loads properly outside of calibration period (validation). Furthermore, the accuracy and precision of model estimations were evaluated using various criteria (e.g., $R^2$ and gradient and constant of linear regression line). The results showed higher precisions with the $R^2$ values closed to 1.0 in LOADEST and better accuracy with the constants (in linear regression line) closed to 0.0 in the eight-parameter model with GDM. In hence, based on these finding we recommend that users need to evaluate the regression models under various criteria and calibration methods to provide the more accurate and precise results for pollutant load estimations.

• Journal of the Korean Society for Industrial and Applied Mathematics
• /
• v.2 no.2
• /
• pp.97-109
• /
• 1998

A comparison of the two dimensional heat loss, computed using the analytical method and the finite difference method in two models(i.e. one is a parabolic fin whose parabolic curves meet at the fin center line and the other is a transformed parabolic fin whose tip cuts vertically), is made assuming the analytical method is correct. For these methods, the root temperature and surrounding convection coefficients of these fins are assumed as constants. The results show that the relative errors of the heat loss between the two methods for the parabolic fin whose tip cuts vertically are smaller than those for the one whose tip does not cut. In case of Bi=0.01, the values of the heat loss obtained using a finite difference method are close to those values obtained using the analytical method for both models. The values of the heat loss from both models calculated by using the analytical method are almost the same for given range of non-dimensional fin length in case of Bi = 0.01 and 0.1.