• 한국환경농학회지
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• 제25권4호
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• pp.371-381
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• 2006

New proteomic techniques have been pioneered extensively in recent years, enabling the high-throughput and systematic analyses of cellular proteins in combination with bioinformatic tools. Furthermore, the development of such novel proteomic techniques facilitates the elucidation of the functions of proteins under stress or disease conditions, resulting in the discovery of biomarkers for responses to environmental stimuli. The ultimate objective of proteomics is targeted toward the entire proteome of life, subcellular localization biochemical activities, and the regulation thereof. Comprehensive analysis strategies of proteomics can be classified into three categories: (i) protein separation via 2-dimensional gel electrophoresis (2-DE) or liquid chromatography (LC), (ii) protein identification via either Edman sequencing or mass spectrometry (MS), and (iii) proteome quantitation. Currently, MS-based proteomics techniques have shifted from qualitative proteome analysis via 2-DE or 2D-LC coupled with off-line matrix assisted laser desorption ionization (MALDI) and on-line electrospray ionization (ESI) MS, respectively, toward quantitative proteome analysis. In vitro quantitative proteomic techniques include differential gel electrophoresis with fluorescence dyes. protein-labeling tagging with isotope-coded affinity tags, and peptide-labeling tagging with isobaric tags for relative and absolute quantitation. In addition, stable isotope-labeled amino acids can be in vivo labeled into live culture cells via metabolic incorporation. MS-based proteomics techniques extend to the detection of the phosphopeptide mapping of biologically crucial proteins, which ale associated with post-translational modification. These complementary proteomic techniques contribute to our current understanding of the manner in which life responds to differing environment.

• Journal of Plant Biotechnology
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• 제37권2호
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• pp.196-204
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• 2010

With the completion of genome sequencing of several organisms, attention has been focused to determine the function and functional network of proteins by proteome analysis. The recent techniques of proteomics have been advanced quickly so that the high-throughput and systematic analyses of cellular proteins are enabled in combination with bioinformatics tools. Furthermore, the development of proteomic techniques helps to elucidate the functions of proteins under stress or diseased condition, resulting in the discovery of biomarkers responsible for the biological stimuli. Ultimate goal of proteomics orients toward the entire proteome of life, subcellular localization, biochemical activities, and their regulation. Comprehensive analysis strategies of proteomics can be classified as three categories: (i) protein separation by 2-dimensional gel electrophoresis (2-DE) or liquid chromatography (LC), (ii) protein identification by either Edman sequencing or mass spectrometry (MS), and (iii) quanitation of proteome. Currently MS-based proteomics turns shiftly from qualitative proteome analysis by 2-DE or 2D-LC coupled with off-line matrix assisted laser desorption ionization (MALDI) and on-line electrospray ionization (ESI) MS, respectively, to quantitative proteome analysis. Some new techniques which include top-down mass spectrometry and tandem affinity purification have emerged. The in vitro quantitative proteomic techniques include differential gel electrophoresis with fluorescence dyes, protein-labeling tagging with isotope-coded affinity tag, and peptide-labeling tagging with isobaric tags for relative and absolute quantitation. In addition, stable isotope labeled amino acid can be in vivo labeled into live culture cells through metabolic incorporation. MS-based proteomics extends to detect the phosphopeptide mapping of biologically crucial protein known as one of post-translational modification. These complementary proteomic techniques contribute to not only the understanding of basic biological function but also the application to the applied sciences for industry.

• BMB Reports
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• 제36권3호
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• pp.269-274
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• 2003

In the fight against cancer, novel chemotherapeutic agents are constantly being sought to complement existing drugs. Various studies have presented evidence that the apoptosis that is induced by these anticancer agents is implicated in tumor regression, and Bcl-2 family genes play a part in apoptosis following treatment with various stimuli. Here, we present data that a styrylpyrone derivative (SPD) that is extracted from the plant Goniothalamus sp. showed cytotoxic effects on the human breast cancer cell line MCF-7. SPD significantly increased apoptosis in MCF-7 cells, as visualized by phase contrast microscopy and evaluated by the Tdt-mediated dUTP nick end-labeling assay and nuclear morphology. Western blotting and immunostaining revealed up-regulation of the proapoptotic Bax protein expression. SPD, however, did not affect the expression of the anti-apoptotic protein, Bcl-2. These results, therefore, suggest SPD as a potent cytotoxic agent on MCF-7 cells by inducing apoptosis through the modulation of Bax levels.

• International Journal of Contents
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• 제8권1호
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• pp.30-38
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• 2012

Text extraction and binarization are the important pre-processing steps for text recognition. The performance of text binarization strongly related to the accuracy of recognition stage. In our proposed method, the first stage based on line detection and shape feature analysis applied to locate the position of a business card and detect the shape from the complex environment. In the second stage, several local regions contained the possible text components are separated based on the projection histogram. In each local region, the pixels grouped into several connected components based on the connected component labeling and projection histogram. Then, classify each connect component into text region and reject the non-text region based on the feature information analysis such as size of connected component and stroke width estimation.

• 무역상무연구
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• 제14권
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• pp.75-102
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• 2000

At present, on increasing to use a computer network and internet network, Internet Trade has been rapidly created and developed in international Electronic Commerce. However, Internet Trade has not been continued to grow up without supporting by a efficient Logistics System. Because it is very important to delivery contract commodities to consumer with speedy, accurate, steady, convenient services. International Logistics System is consisted of demand forecasting, order processing, packing, labeling, shipping documentation and consumer service into three distant phases of transaction, distribution and payment. International Logistics System can be done more efficiently and effectively by using Internet Logistics System. Therefore it is very important to implementing a efficient Internet Logistics System.

• 반도체디스플레이기술학회지
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• 제9권2호
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• pp.79-85
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• 2010

In this paper, we propose a web inspection system (WIS) for real-time detection of paper defects which can cause critical fractures during papermaking process. Our system incorporates high speed line-scan camera, lighting system, and detection algorithm to provide robust and precise detection of paper defects in real-time. Since edge defects are very crucial to the paper fractures, our system focuses on the edge region of the paper instead of inspecting the whole paper area. In our algorithm, image projection and sub-pixel operation are utilized to detect the edge defects precisely and connected component labeling and shape analysis techniques are adopted to extract various kinds of the region defects. Experimental results revealed that our web inspection system is very efficient for detecting paper defects during papermaking processes.

• 한국CDE학회논문집
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• 제16권4호
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• pp.249-259
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• 2011

In this paper we present our work on the parameterized construction of virtual drivers' reach motion to seat belt, by using motion capture data. A user can generate a new reach motion by controlling a number of parameters. We approach the problem by using multiple sets of example reach motions and learning the relation between the labeling parameters and the motion data. The work is composed of three tasks. First, we construct a motion database using multiple sets of labeled motion clips obtained by using a motion capture device. This involves removing the redundancy of each motion clip by using PCA (Principal Component Analysis), and establishing temporal correspondence among different motion clips by automatic segmentation and piecewise time warping of each clip. Next, we compute motion blending functions by learning the relation between labeling parameters (age, hip base point (HBP), and height) and the motion parameters as represented by a set of PC coefficients. During runtime, on-line motion synthesis is accomplished by evaluating the motion blending function from the user-supplied control parameters.

• 대한전자공학회논문지SP
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• 제40권3호
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• pp.217-226
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• 2003

본 논문에서는 세선화 지문 영상의 순차적 레이블링을 이용하여 위치 이동, 크기 변화 그리고 회전에 무관한 새로운 지문 융선 특징 검출 알고리즘을 제안한다. 제안한 알고리즘은 먼저 지문의 중심점을 지나는 수직선을 이용하여 세선화 지문 영상의 융선을 순차적으로 레이블링 한다. 그리고 레이블링한 개개의 융선들로부터 특징을 검출한다 검출하는 특징은 융선의 종류와 융선에 존재하는 특징점의 융선 각도이다. 이러한 방법을 이용하여 지문 융선의 특징을 검출하면, 지문을 이루고 있는 여러 융선들의 종류를 알 수 있고, 각 융선에 존재하는 특징점의 종류 및 이들의 각도를 알 수 있다. 두 개의 세선화 지문 영상을 이용하여 실험한 결과, 제안하는 알고리즘이 위치 이동, 크기 변화 그리고 회전에 무관한 지문 융선 특징을 검출함을 확인하였다.

• Journal of Microbiology and Biotechnology
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• 제14권6호
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• pp.1150-1156
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• 2004

The GMO (Genetically Modified Organism) labeling system on raw materials has been in Korea since March 2001, and genetically modified organisms (GMOs)-derived foods since July 2001. Therefore, we designed a multiplex PCR method to ascertain the validity of the labeling system and to monitor the status of circulation for genetically modified maize (GM Maize). Five lines of GM Maize (GA21, TC1507, Mon810, NK603, and Bt176) were used, and specific primer pairs were designed to detect each line. Using this method, the different lines of GM Maize were monitored from raw products and processed foods in Korean market. Some of the maize processed foods and raw materials were shown to contain more than one foreign gene. This method was found to be effective for-detecting five different GM Maize in a single reaction.

• 한국자기공명학회논문지
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• 제14권2호
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• pp.117-126
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• 2010

Hsp33 is a molecular chaperone achieving a holdase activity upon response to a dual stress by heat and oxidation. Despite several crystal structures available, the activation process is not clearly understood, because the structure inactive Hsp33 as its reduced, zinc-bound, monomeric form has not been solved yet. Thus, we initiated structural investigation of the reduced Hsp33 monomer by NMR. In this study, to overcome the high molecular weight (33 kDa), the protein was triply isotope-[$^{13}C$, $^{15}N$, $^2H$]-labeled and its inactive, monomeric state was ensured. 2D-[$^1H$, $^{15}N$]-TROSY and a series of triple resonance spectra could be successfully obtained on a high-field (900 MHz) NMR machine with a cryoprobe. However, under all of the different conditions tested, the number of resonances observed was significantly less than that expected from the amino acid sequence. Thus, a possible contribution of dynamic conformational exchange leading to a line broadening is suggested that might be important for activation process of Hsp33.