• Food Science and Biotechnology
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• 제17권2호
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• pp.373-378
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• 2008

Contents of phytochemicals in citron (Citrus junos Sieb. ex Tanaka) seeds and their effects on antioxidant activities were investigated. Methanol extract of defatted citron seeds contained the highest amounts of total polyphenols followed by 70% ethanol and water extracts. Neohesperidin was the most predominant citrus flavanones in these extracts. The highest amounts of limonoids were found in methanol extract, and this extract was the most efficient in scavenging both DPPH and ABTS radicals. All 3 extracts also exhibited good antioxidant activities against attack of linoleate free radicals on $\beta$-carotene. When methanol extract was sequentially fractionated into ether, ethyl acetate, n-butanol, and water fractions, butanol fraction contained the highest amounts of polyphenols otherwise most of limonoids were concentrated in ethyl acetate fraction. A positive relationship between radical scavenging activities and total polyphenol contents in fractions was observed while antioxidant activity on $\beta$-carotene seemed more related with contents of limonoids and other hydrophobic polyphenols.

• Natural Product Sciences
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• 제26권1호
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• pp.64-70
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• 2020

Citrus junos seeds (CS) have been traditionally used for the treatment of cancer and neuralgia. They are also used to manufacture edible oil and cosmetic perfume. A large amount of CS shells without oil (CSS) are discarded after the oil in CS is used as foods or herbal remedy. To efficiently utilize CSS as a by-products, it needs to be studied through chemical analysis. Therefore, we developed an ultra-performance liquid chromatography (UPLC)-diode array detection (DAD) method for simultaneous determination and quantitative analysis of five components (two flavonoids and threes limonoids) in CSS. A Waters Acquity UPLC HSS T3 column C18 (2.1 × 100 mm, 1.8 ㎛) was used for this separation. It was maintained at 40 ℃. The mobile phase used for the analysis was distilled water and acetonitrile with gradient elution. To identify the quantity of the five components, a mass spectrometer (MS) with an electrospray ionization (ESI) source was used. The regression equation showed great linearity, with correlation coefficient ≥ 0.9912. Limits of detection (LOD) and limits of quantification (LOQ) of the five compounds were 0.09 - 0.13 and 0.26 - 0.38 ㎍/mL, respectively. Recoveries of extraction ranged from 97.45% to 101.91%. Relative standard deviation (RSD) values of intra- and inter-day precision were 0.06 - 1.15% and 0.19 - 0.25%, respectively. This UPLC-DAD method can be validated to simultaneously analyze quantities of marker flavonoids and limonoids in CSS.

• 한국자원식물학회지
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• 제11권
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• pp.116-116
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• 1998

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 1996년도 International Symposium on the Development of Anti Cancer Resources From Plants및 1996년도 한국자원식물학회 정기학술 발표회
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• pp.16-26
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• 1996

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 1998년도 천연항암자원개발에 관한 국제학술회의 논문집 및 1998년도 한국자원식물학회 정기학술발표회 논문요약
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• pp.116-116
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• 1998

• Archives of Pharmacal Research
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• 제25권6호
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• pp.824-830
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• 2002

Four flavonoid glycosides, flavaprin (7), evodioside B (8), vitexin (11), and hesperidin (12), as well as the coumarins bergapten (1), xanthotoxin (2), and isopimpinellin (3), the lignan simplexoside (10), the steroids ${\beta}-sitosterol$ (4) and daucosterol (5), the limonoids isolimonexic acid (6) and limonin (9), and uracil (13) and myo-inositol (14) have been isolated from Euodia daniellii. The structures of these compounds were established from spectral data. Among the isolates, bergapten showed cyclooxygenase-2 inhibitory activity with an $IC_{50}$ value of $6.2{\;}{\mu\textrm{g}}/ml. Flavonoids isolated from this plant exhibited no cytotoxic activity against the human tumor cell lines, A549, SKOV-3, SKMEL-2, XF498, and HCT15.

• Natural Product Sciences
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• 제5권2호
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• pp.65-69
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• 1999

By bioassay guided fractionation followed by chromatographic separation of the MeOH extract from the fruit of Evodia rutaecarpa (Juss.) Benth. (Rutaceae), a new cyclooxygenase-2 inhibitor was isolated and identified as an alkaloid, rutaecarpine. Other alkaloids such as evodiamine and dehydroevodiamine together with limonoids were also isolated and characterized.

• 대한약학회:학술대회논문집
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• 대한약학회 2001년도 Proceedings of International Convention of the Pharmaceutical Society of Korea
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• pp.264.1-264.1
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• 2001

• 한국응용과학기술학회지
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• 제29권4호
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• pp.610-617
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• 2012

탱자에는 여러 종류의 monoterpenes, limonoids, flavonoids과 coumarins 등의 구성성분으로 이루어져 있다. 탱자의 구성 성분중에 7-제라닐옥시쿠마린는 7개의 탄소로 구성된 제라닐옥시기의 곁사슬을 가진 물질이다. 그리고 7-제라닐옥시쿠마린은 여러 약리적인 효과들을 나타낸다고 알려져 있다. 본 연구에서는 탱자의 구성성분인 7-제라닐옥시쿠마린과 이들의 다양한 유도체를 합성하였다. 그리고 항염증에 대한 항염증 효능을 알아보기 위하여 염증을 유발하는 일산화질소 억제 cytokine을 측정한 결과는 6-제라닐옥시쿠마린의 성분이 사이토카인인 인터루킨-6가 $1{\mu}M$농도에서 68.9% 를, $10{\mu}M$에서 72.6% 의 저해효과를 나타냈다.

• Journal of Applied Biological Chemistry
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• 제48권2호
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• pp.93-96
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• 2005

Three limonoids and two phenolics were isolated from stem bark of Pellodendron amurense Rupr. (Rutaceae) and were defined clearly as calodendrolide (1), obacunone (2), limonin (3), methyl 4-hydroxybenzoate (4), and syringin (5) based on NMR spectroscopy results obtained with the aid of X-ray crystallographic analysis. This is the first report on the isolation of calodendrolide (1) from this plant.