• Korean Journal of Medicinal Crop Science
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• v.27 no.1
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• pp.17-23
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• 2019

Background: Cnidium officinale Makino and Ligusticum chuanxiong Hort. are important medicinal crops in Korea. However, there is insufficient information on the identification of thrips, which attack these plants. Until now, one species of thrips has been recorded as a main pest. Methods and Results: To identify the thrips emerging in C. officinale Makino and L. chuanxiong Hort., these plants were independently cultivated in two local areas. Thirty individuals of each plant species were selected randomly and surveyed for the presence of thrips. After confirming the existence of thrips, 100 thrips individuals were collected from each crop using the beating method. To identify thrips species, we performed PCR-restriction fragment length polymorphism (RFLP)-based analysis using ITS2 primer sets. Six thrips species were identified: western flower thrips (Frankliniella occidentalis), flower thrips (F. intonsa), onion thrips (Thrips tabaci), chrysanthemum thrips (T. nigropilosus), chilli thrips (Scirtothrips dorsalis), and grass thrips (Anaphothrips obscurus). The proportion of these species differed between the host plant species. Conclusions: Six thrips species were major pests of two medicinal crops. Integrated pest management is required to control these thrips species, and will enhance the yield and quality of C. officinale and L. chuanxiong.

• The Korean Journal of Mycology
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• v.47 no.4
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• pp.329-335
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• 2019

Endophytic fungal strains were isolated from the sterilized roots of a medicinal plant, Ligusticum chuanxiong and identified based on morphological characteristics and molecular analysis of internal transcribed spacer, large subunit rDNA, and beta-tubulin DNA regions. Our results confirmed the presence of Pithomyces chartarum and Plectosphaerella niemeijerarum in the fungal strains. To the best of our knowledge, the fungal strains have not been reported in Korea. In this report, we describe morphological characteristics and phylogenetic trees of these endophytic fungal strains.

• The Korea Journal of Herbology
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• v.20 no.4
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• pp.95-101
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• 2005

Objectives : In order to distinguish Cnidii Rhizoma and Ligustici Chuanxiong Rhizoma (cultivated in Korea and China), their external and internal states were observed via microscopic examination. Methods : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and then observed and photographed by olymphus-BHT. Results : Cnidii Rhizoma, Ligustici Chuanxiong Rhizoma (from Korea), and Ligustici Chuanxiong Rhizoma (from China) all demonstrated similar patterns as following : 1. external form 1) showing irregular nodular form with $2{\sim}7cm$ diameter. 2) having several trochites in parallel, and nodulose root trace on surface. 3) in addition to yellow-white or yellow-gray intersections, yellow-brown oil sacs were distributed, with cambium resembling a form of annulus. 2. internal form 1) its root and rhizome is composed of 10 layers of cork, with narrow cortical layers. 2) its phloem is wide, and oval light yellow-brown oil sacs are distributed around. The closer to the cambium, the smaller it is. 3) cambium is a form of a wave-like ring or an irregular polygon. 4) xylem and vessel are rare, most of which are arranged in a 'V' format, and sometimes xylem fiber is found. 5) pith is composed of parenchymatous cell, and parenchyma is full of starch grains. Conclusions : In conclusion, C officinale, L. chuanxiong (from Korea), and L. chuanxiong (from China) have rhizomes in a similar form with no special difference. Therefore, it is concluded that C. officinale, L. chuanxiong (from Korea), and L. chuanxiong (from China) are categorized into the same species.

• Journal of Food Hygiene and Safety
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• v.26 no.4
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• pp.370-376
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• 2011

This study was performed to investigate the antioxidative effect of Ligusticum chuanxiong Hort extracts (LCE) against the hyperlipidemia of high-fat diet-fed obese rats. The rats were divided into the three groups (normal group, control group and sample group) to perform the experimental research. 1.5 ml/kg of LCE was intraperitoneally administered into the sample group for 21 days. The equal dose of 0.9% saline was intraperitoneally administered into the normal group and the control group. On day 22, they were anesthetized with ether and dissected. The levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) were examined in serum of rats. Superoxide dismutase (SOD) was measured in mitochondrial fraction. Malondialdehyde (MDA), catalase (CAT), and glutamate peroxidase (GPx) were determined in liver homogenate. High-fat diet markedly increased the levels of AST, ALT and MDA, significantly decreasing those of SOD, CAT and GPx. But Ligusticum chuanxiong Hort-pretreatment decreased the levels of AST, ALT, and MDA. increasing those of SOD, CAT and GPx. These results demonstrated the antioxidative effects, suggesting that LCE could be the candidate for the functional material.

• Korean Journal of Medicinal Crop Science
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• v.10 no.5
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• pp.415-418
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• 2002

Ligusticum chuanxiong Hort. is one of the most important root-using medicinal crops in Korea. A new variety 'Sinto' (Suwon 2) was developed from the population of Muju local through phenotyptic restricted mass selection at National Crop Experiment Station, RDA. in 2001. The average yield of this variety was about 2,260 kg/ha in RYT at three location from 1999 to 2001 which were about 20% higher than that of Pyeongchang local. Sinto showed a strong resistance to powdery mildew. This variety may be well adaptable to mountainous mid-southern areas in Korean peninsula.

• Korean Journal of Medicinal Crop Science
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• v.5 no.1
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• pp.1-6
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• 1997

The experiment was conducted to investigate the effect of water stress on yield and quality of Ligusticum chuanxiong Hort. The water stress treatment was imposed artificially on seedling, flowering and rhizome enlargement stage of the plant. The root yield rate decreased to 19.1%, 18.2% and by the water stress treatment at rhizome enlargement, seedling and flowering stage, respectively. Portion of the products having commercial quality grade (above 20g of rhizome weight) was 93.4% at control plot, while it was 85%, 81.7% and 78.3% when stressed for water at seedling, flowering and root enlargement stage, respectively. Content of extract was the higher in the order of control, water stressed at rhizome enlargement, flowering and the seedling stage. Postive correlationship was found between yield of rhizome and rootlet yield or economic production ratio, and between dry weight of stem and rootlet yield.

• Korean Journal of Medicinal Crop Science
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• v.7 no.3
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• pp.200-204
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• 1999

Ligusticum chuanxiong has been an important oriental crude drug and cultivated for a long time in Korea. Ligusticum chuanxiong has been vegetatively propagating crop without seed due to its sterility and would be an allodiploid derived from the hybridization of two allied species. Generally, vegetative propagation is more costly per unit propagule than sexual(seedling) propagation. This study was conducted to find out the techniques of mass propagation by stem cutting for reduced seedling cost. When cutted on August 30 with 1st to 2nd internode, its highest rooting percentage was 81 percent. Rhizome yield of plants cutted in 50 percent on September 1 decreased to 9 percent less than control (rhizome propagation). Rooting percentage of rooted cutting plants after field planting on October 25 was the highest being 87 percent. Rhizome yield with planting appeared 127 and 120kg/10a on September 25 and October 25, respectively.

• Korean Journal of Medicinal Crop Science
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• v.6 no.1
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• pp.57-61
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• 1998

Angelica gigas, Astragalus membranaceus and Ligusticum chuanxiong have been grown for a long time in Korea as medicinal crops with underground parts. Its harvesting method has been depended entirely on manual labor. Therefore, harvesting involved much work. This study was to determine an effective mechanized harvesting method for underground parts of some medicinal crops by several machines. Labor time was decreased by 61 percent in Angelica gigas and by 70 percent in Astragalus membranaceus by the use of poclain harvester, however, in Ligusticum chuanxiong was decreased 68 percent by multi - root harvester compared with conventional system (manual harvest). The poclain harvester was suitable for harvesting in Angelica gigas and Astragalus membranaceus plots, but multi - root harvester was not satisfactory. Multi - root harvester appeared to be appropriate harvester for Ligusticum chuanxing.

• Korean Journal of Pharmacognosy
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• v.47 no.2
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• pp.128-136
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• 2016

Tiny leaf fragments were found in the Annals of the Joseon Dynasty, which were compiled about 500 years ago. The records describing the detailed process of compiling the Annals indicate that silk bags packed with the powders of 'Cheongung' and 'Changpo', which have been used as traditional herbal medicines in the northeast Asian countries such as China and Japan as well as Korea, were put in the wooden storage boxes together with the volumes of the Annals. However, there is no record that parts of plants were used in the process of compiling the Annals. The botanical origin of leaf fragments was identified as Ligusticum sinense 'Chuanxiong' by the analysis of trnK of chloroplast DNA as well as the examination of leaf surface with SEM. The comparative analysis of trnK sequences showed that the chloroplast DNA haplotype of 'Tocheongung', a triploid species cultivated in Korea, was identical with Cnidium officinale, but different from L. sinense 'Chuanxiong'. The molecular results provide a new suggestion on the botanical origin of crude drugs used as 'Cheongung', which has been disputed in Korea.

• Korean Journal of Medicinal Crop Science
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• v.3 no.3
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• pp.246-250
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• 1995

Scions from the one- and two-years old and the nodes of Ligusticum chuanxiong stem were used to study the rooting ability with the treatment of plant growth regulators planted to different bed soils as the alternative propagation for the present method by rhizome. The rooting ratio in the scion from the one- and the two-years old plants showed any difference, but that was ranged from 94% to 100% in the first node and from 56% to 64% in the second node from the bottom of the stem. The scion of first node from both the plants showed more than 90% in the root ratio and 16 in the number of rooting and growed more than 31cm in the root length. The Rootone-F and 250ppm of NAA were most effective for the promotion of rooting. Rooting ratios in the sand and in the mixture of vermiculite and perlite as bed soils were about 18%, but that in the cultured soil considerably decreased.