Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Molecular Identification of Thrips in Two Medicinal Crops, Cnidium officinale Makino and Ligusticum chuanxiong Hort

일천궁과 토천궁에서 발생하는 총채벌레류의 분자동정

  • 정충렬 (국립산림과학원 산림약용자원연구소) ;
  • 정대희 (국립산림과학원 산림약용자원연구소) ;
  • 박홍우 (국립산림과학원 산림약용자원연구소) ;
  • 김현준 (국립산림과학원 산림약용자원연구소) ;
  • 전권석 (국립산림과학원 산림약용자원연구소) ;
  • 윤정범 (국립원예특작과학원)
  • Received : 2018.11.27
  • Accepted : 2019.01.21
  • Published : 2019.02.28
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Abstract

Background: Cnidium officinale Makino and Ligusticum chuanxiong Hort. are important medicinal crops in Korea. However, there is insufficient information on the identification of thrips, which attack these plants. Until now, one species of thrips has been recorded as a main pest. Methods and Results: To identify the thrips emerging in C. officinale Makino and L. chuanxiong Hort., these plants were independently cultivated in two local areas. Thirty individuals of each plant species were selected randomly and surveyed for the presence of thrips. After confirming the existence of thrips, 100 thrips individuals were collected from each crop using the beating method. To identify thrips species, we performed PCR-restriction fragment length polymorphism (RFLP)-based analysis using ITS2 primer sets. Six thrips species were identified: western flower thrips (Frankliniella occidentalis), flower thrips (F. intonsa), onion thrips (Thrips tabaci), chrysanthemum thrips (T. nigropilosus), chilli thrips (Scirtothrips dorsalis), and grass thrips (Anaphothrips obscurus). The proportion of these species differed between the host plant species. Conclusions: Six thrips species were major pests of two medicinal crops. Integrated pest management is required to control these thrips species, and will enhance the yield and quality of C. officinale and L. chuanxiong.

Keywords

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Fig. 1. Gel-electrophoresis of ITS2-PCR products using ITS2 primer on genomic DNA from the six thrips species (A) and PCR-RFLP using Rsa I (B). M; 1,000 bp (A) and 100 bp ladder (B).

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Fig. 2. The distribution ratios of six thrips species in two medicinal crops, C. officinale (A) and L. chuanxiong (B).

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Fig. 3. Morphology of A. obscurus (A) and T. nigropilosus (B) under the stero microscope.

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Fig. 4. Phylogenetic trees of A. obscurus (A) and T. nigropilosus (B).

Table 1. The geographical information on thrips collection.

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