• Title/Summary/Keyword: Light weight body

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Changes in expression of the autophagy-related genes microtubule-associated protein 1 light chain 3β and autophagy related 7 in skeletal muscle of fattening Japanese Black cattle: a pilot study

  • Nakanishi, Tomonori;Tokunaga, Tadaaki;Ishida, Takafumi;Kobayashi, Ikuo;Katahama, Yuta;Yano, Azusa;Erickson, Laurie;Kawahara, Satoshi
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.4
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    • pp.592-598
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    • 2019
  • Objective: Autophagy is a bulk degradation system for intracellular proteins which contributes to skeletal muscle homeostasis, according to previous studies in humans and rodents. However, there is a lack of information on the physiological role of autophagy in the skeletal muscle of meat animals. This study was planned as a pilot study to investigate changes in expression of two major autophagy-related genes, microtubule-associated protein 1 light chain $3{\beta}$ (MAP1LC3B) and autophagy related 7 (ATG7) in fattening beef cattle, and to compare them with skeletal muscle growth. Methods: Six castrated Japanese Black cattle (initial body weight: $503{\pm}20kg$) were enrolled in this study and fattened for 7 months. Three skeletal muscles, M. longissimus, M. gluteus medius, and M. semimembranosus, were collected by needle biopsy three times during the observation period, and mRNA levels of MAP1LC3B and ATG7 were determined by quantitative reverse-transcription polymerase chain reaction. The expression levels of genes associated with the ubiquitin-proteasome system, another proteolytic mechanism, were also analyzed for comparison with autophagy-related genes. In addition, ultrasonic scanning was repeatedly performed to measure M. longissimus area as an index of muscle growth. Results: Our results showed that both MAP1LC3B and ATG7 expression increased over the observation period in all three skeletal muscles. Interestingly, the increase in expression of these two genes in M. longissimus was highly correlated with ultrasonic M. longissimus area and body weight. On the other hand, the expression of genes associated with the ubiquitin-proteasome system was unchanged during the same period. Conclusion: These findings suggest that autophagy plays an important role in the growth of skeletal muscle of fattening beef cattle and imply that autophagic activity affects meat productivity.

Assessment of Physical Activity, Activity Coefficient of Preschool Children and Actual Condition of Daycare Center Outdoor Play (미취학아동의 1일 활동량, 활동계수 평가 및 유아보육시설의 실외놀이 실태)

  • Kim, Jae-Hee;Kim, Eun-Kyung
    • Korean Journal of Community Nutrition
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    • v.14 no.6
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    • pp.777-788
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    • 2009
  • The purpose of this study was to assess physical activities and activity coefficient of preschool children and was to give some concrete information to activate outdoor play and to probe the suggestions to activate outdoor play. 42 preschool children (17 boys and 25 girls) were included. Body weight and height were measured and one-day activity diaries were collected by interviewing with their parents and kindergarten teachers. To measure physical activity during outdoor play, this study was researched the current conditions and content of outdoor play activity in daycare centers, and teacher's perceptions of that outdoor play. The average age of the subjects was 5.0 years. Average height, weight, body fat and body muscle of subjects were 108.9 cm, 18.9 kg, 17.7% and 26.8%, respectively. The subjects spent about 10 hours 39 minutes sleeping; 4 hours 16 minutes personal hygiene and computer working. They spent 88.2% of 24 hours (one day) in "very light activities" and 11.3% in "light activities". Activity coefficient (1.34) of weekday was significantly higher than that of weekend (1.21). And in this study, it was found that most of daycare centers (87.7%) have outdoor play space, 95.2% of them were garden. The teachers recognized that have a outdoor play frequency everyday (48%), three or four times (46%) in a week but they have three or four times (38%), one or two times (30%) because of various indoor program. Also, they recognized that have a outdoor play expending time 21-30 minutes (48%), 31-40 minutes (26%) but they have 21-30 minutes (64%). They answered that have frequency and expending time less than their recognition of outdoor play. Most of daycare center teachers perceived outdoor play to be as important as indoor play, and the teachers believed outdoor play supports children's physical, cognitive, social, and language development. The results of this study may be used to utilize as a basic data for estimate physical activity for preschool children and developed that exercise program to increase physical activity of daycare center outdoor play.

Studies on Development of Reticulum and Abomasum with Age, and Their Relationship in Korean Native Young Goats (한국 재래유산양(幼山羊)의 제2위와 제4위의 일령 별 발달과 상호관계에 관한 연구)

  • Ha, J.K.;Lee, J.G.;Chang , H.H.;Kim, B.W.
    • Journal of Animal Science and Technology
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    • v.45 no.6
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    • pp.967-974
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    • 2003
  • This experiment was carried out to investigate the relationship of morphological characteristics between reticulum and abomasum of Korean native young goats age from 2 days to 150 days. Number of traits investigated in the reticulum in this experiment were 12[body weight, chest girth, body length, right and left reticulum length(R.L.), upper and lower reticulum length(U.L.), reticulum weight(R.W.), reticulum area(R.A.), upper and lower length of one polygon located at central part of reticulum(U.P.C.R.), right and left length of one polygon located at central part of reticulum(R.P.C.R.), thickness of polygon wall located at central part of reticulum(T.P.C.R.), thickness of polygon wall located at middle part of reticulum(T.P.M.R.), and thickness of polygon wall locared at edge part of reticulum(T.P.E.R.)] and items for abomasum were 12[length of between ostium omaso-abomasicum part and pylosica part in the abomasum(L.B.O.P.), broadest outer part of the abomasum(B.O.A.), weight of abomasum(W.O.A.), area of abomasum(A.O.A.), number of plicae abomasi in the abomasum(N.P.A.A.), thickness of abomasum well at cranial part(ostium omasoabomasicum) in the abomasum(T.A.C.A.), thickness of abomasum well at central part in the abomasum(T.A.P.A.), thickness of abomasum wall at light upper area of pylosica part in the abomasum(T.A.L.A.), length measured from the longest plica abomasi in the abomasum(L.L.P.A.), broadest measured from the longest plica abomasi in the abomasum(B.L.P.A.), area measured from the longest plica abomasi in the abomasum(A.L.P.A.), weight of longest plica abomasi in the abomasum(W.L.P.A.)]. The results were summarized as follows: 1. Number of coefficient of correlation obtained among 12 traits of the abomasum and 12 of the reticulum were 144, and coefficient of correlation of 114 were significant(P〈0.05). 2. Trait of abomasum weight have high correlation with 12 traits of reticulum. 3. Correlation coefficients and regression equation between body weight. VS. abomasum weight(r$_1$), and upper and lower length of one polygon located at central part of reticulum(U.P.C.R.) VS. abomasum weight(r$_2$) were r$_1$=0.8954$^{**}$ and Y=10.703+3.374X, r$_2$=0.8430$^{**}$ and Y=5.689+4.311X, respectively. 4. Correlation coefficients and regression equation between chest girth VS. abomasum weight(r$_1$), and body weight VS. abomasum weight(r$_2$) were r$_1$=0.8708$^{**}$ and Y=-17.219+1.227X, r$_2$=0.8589$^{**}$ and Y=- 17.616+1.290X, respectively.

Methods of Merging a 3D Replica and Ease Distribution for Woman's Pant Patterns (여성용 바지 패턴 설계를 위한 3차원 밀착 패턴 여유량 부가와 레플리카 조합 방법)

  • Wu, Yanjun;Hong, Kyung-Hi
    • Journal of the Korean Society of Clothing and Textiles
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    • v.36 no.4
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    • pp.443-455
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    • 2012
  • In this research, a woman matched to the average Korean standard size specification of 21-24 years of age was scanned using Cyberware. Rapidform 2004 was used to separate the body zone into fit zone, action zone and design zone, depending on the function of the lower body. Each divided body zone expanded radially using 'offset' by 1.60mm at the cross section of the waist, and 6.36mm at the hip level. Resultant ease values were 1cm and 4cm along the waistline and hipline (respectively), as recommended in previous research. 2C-AN program and Yuka CAD was used to develop a pant pattern from the enlarged 3D body surface blocks. A total of five pants were constructed using conventional fabrics for light weight formal pants. The appearance of the experimental pants were evaluated by five clothing-major evaluators using a questionnaire that consisted of 21 questions. In addition, functional aspects of the experimental pants were also evaluated by wearer while performing five postures for the measurement of ease of movement. It was found that, the method of regional offset and suggested 3D to 2D pattern development generate reasonably good pant patterns. Among the pattern block arrangements, the method B was evaluated as the best way to generate formal pants from the tight-fit pattern, which fit smoothly over the lower body for maintaining ease of movement.

Development of Statistical Model and Neural Network Model for Tensile Strength Estimation in Laser Material Processing of Aluminum Alloy (알루미늄 합금의 레이저 가공에서 인장 강도 예측을 위한 회귀 모델 및 신경망 모델의 개발)

  • Park, Young-Whan;Rhee, Se-Hun
    • Journal of the Korean Society for Precision Engineering
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    • v.24 no.4 s.193
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    • pp.93-101
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    • 2007
  • Aluminum alloy which is one of the light materials has been tried to apply to light weight vehicle body. In order to do that, welding technology is very important. In case of the aluminum laser welding, the strength of welded part is reduced due to porosity, underfill, and magnesium loss. To overcome these problems, laser welding of aluminum with filler wire was suggested. In this study, experiment about laser welding of AA5182 aluminum alloy with AA5356 filler wire was performed according to process parameters such as laser power, welding speed and wire feed rate. The tensile strength was measured to find the weldability of laser welding with filler wire. The models to estimate tensile strength were suggested using three regression models and one neural network model. For regression models, one was the multiple linear regression model, another was the second order polynomial regression model, and the other was the multiple nonlinear regression model. Neural network model with 2 hidden layers which had 5 and 3 nodes respectively was investigated to find the most suitable model for the system. Estimation performance was evaluated for each model using the average error rate. Among the three regression models, the second order polynomial regression model had the best estimation performance. For all models, neural network model has the best estimation performance.

Effect of Leg Stiffness on the Running Performance of Milli-Scale Six-Leg Crawling Robot with Payload (소형 6족 주행 로봇의 페이로드와 다리 강성이 로봇의 주행 성능에 미치는 영향)

  • Chae, Soo-Hwan;Baek, Sang-Min;Lee, Jongeun;Yim, Sojung;Ryu, Jae-Kwan;Jo, Yong-Jin;Cho, Kyu-Jin
    • The Journal of Korea Robotics Society
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    • v.14 no.4
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    • pp.270-277
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    • 2019
  • Inspired by small insects, which perform rapid and stable locomotion based on body softness and tripod gait, various milli-scale six-legged crawling robots were developed to move rapidly in harsh environment. In particular, cockroach's leg compliance was resembled to enhance the locomotion performance of the crawling robots. In this paper, we investigated the effects of changing leg compliance for the locomotion performance of the small light weight legged crawling robot under various payload condition. First, we developed robust milli-scale six-leg crawling robot which actuated by one motor and fabricated in SCM method with light and soft material. Using this robot platform, we measured the running velocity of the robot depending on the leg stiffness and payload. In result, there was optimal range of the leg stiffness enhancing the locomotion ability at each payload condition in the experiment. It suggests that the performance of the crawling robot can be improved by adjusting stiffness of the legs in given payload condition.

Development of Hollow Fibers for the Controlled Release of Drugs

  • Feijen, J.;Eenink, M.J.D.;Olijslager, J.;Schakenraad, J.M.;Nieuwenhuis, P.;Molenaar, I.
    • Journal of Biomedical Engineering Research
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    • v.7 no.2
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    • pp.111-112
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    • 1986
  • Radiation-induced fibrosarcoma tumors were grown on the flanks of C3H mice. The mice were divided into two groups. One group was injected with Photofrin II, intravenously (2.5mg/kg body weight). The other group received no Photofrin II. Mice from both groups were irradialed for approximately 15 minutes at 100, 300, or 500 mW/cm2 with the argon (488nm/514.5 nm), dye(628nm) and gold vapor (pulsed 628 nm) laser light. A photosensitizer behaved as an added absorber. Under our experimental conditions, the presence of Photolfrin II increased surface temperature by at least 40% and the temperature rise due to 300 mW/cm2 irradiation exceeded values for hyperthermia. Light and temperature distributions with depth were estimated by a computer model. The model demonstrated the influence of wavelength on the thermal process and proved to be a valuable tool to investigate internal temperature rise.

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The Effects of Light Colors on the Behavior and Performance of Broiler Chickens (조명의 색이 육계의 행동과 생산성에 미치는 영향)

  • Son, Jang-Ho;Ravindran, Velmurugu
    • Korean Journal of Poultry Science
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    • v.36 no.4
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    • pp.329-335
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    • 2009
  • The aim of the present study was to investigate the behavior and performance of male and female broiler chickens reared under three different colors of light (white, blue and red) in a 23L:1D of lighting program. Day-old broiler chicks were obtained and assigned to six pens (16 males and 16 females per pen). Two pens each were located in three different rooms in an environmentally controlled shed and received the different light colors. All birds received same husbandry conditions. After a 3-day adaptation to the light color, behavior observations (pecking, resting, standing and walking) were made on six focal birds per pen, representing the sex ratio, by direct visual scans for 120 minutes daily for five days a week throughout the 5-week study. Body weights and feed intake were recorded at weekly intervals. On days 19 and 33, welfare indicators (tonic immobility reaction on the 19 and 33 days old; gait scores, foot pad and hock burns scores on 33 days old) were determined. Light color had no significant effect (P>0.05) on the weight gain and feed intake of broilers, but significantly (P<0.05) influenced the feed conversion ratio. Birds receiving the blue light were more (P<0.05) efficient than those receiving white and red lights. Resting was observed to be the major behavior pattern, irrespective of sex or light color. During early growth, resting behavior tended to be lower in birds receiving red light. However, standing and walking behaviors tended to be higher in birds receiving red light in 4 to 18 day-old age and all of experimental periods, respectively. These data indicate that light color can influence broiler performance and behavior.

Effect of Herbal Mixture including Lycii Fructus (HML) on Stress Relief and Melatonin in Caffeine and Light induced Stress Animal Model (Caffeine 및 광자극 유발 스트레스 동물모델에서 구기자 복합물이 스트레스 완화 및 혈중 멜라토닌에 미치는 영향)

  • Kim, Ee-Hwa;Hwang, Dong-Suk;Kim, Hee-Taek;Kim, Yong-Min
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.33 no.4
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    • pp.44-54
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    • 2020
  • Objectives : In the present study, we investigated whether or not an herb mixture including Lycii fructus(HML) has attenuation effects on light stress in rats. Methods : Light stress was induced by exposure to 300 lx of constant lifht for 24 h and 1mg/kg of caffeine was injected into the rat. Rats were orally treated with distilled water of HML for 21 days at 100mg/kg/d. The changes of mortality rate, clinical signs, body, blood chemistry, melatonin and IL-2 expression were analyzed after experiment. Results : Normal weight changes were observed in all treatment groups. In addition, no changes were observed in dead animals and general symptoms. At the time of autopsy, the appearance of all groups and gross examination of major organs were observed, and no gross findings were observed. Blood biochemical tests showed that total cholesterol and HDL cholesterol levels were statistically significantly decreased in the group treated with HML compared to the stress-induced group. Melatonin was measured in the blood, but there was no significant difference in the group treated with HML compared to the group with stress-induced group, but it tended to increase. IL-2 was measured in the blood, no significant change was observed in the group treated with HML compared to the stress-induced group. Conclusions : According to the above results, HML maybe help with hematological changes expressed by caffeine and light-induced stress.

Transparent and Flexible All-Organic Multi-Functional Sensing Devices Based on Field-effect Transistor Structure

  • Trung, Tran Quang;Tien, Nguyen Thanh;Seol, Young-Gug;Lee, Nae-Eung
    • Proceedings of the Korean Vacuum Society Conference
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    • 2011.02a
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    • pp.491-491
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    • 2011
  • Transparent and flexible electronic devices that are light-weight, unbreakable, low power consumption, optically transparent, and mechanical flexible possibly have great potential in new applications of digital gadgets. Potential applications include transparent displays, heads-up display, sensor, and artificial skin. Recent reports on transparent and flexible field-effect transistors (tf-FETs) have focused on improving mechanical properties, optical transmittance, and performances. Most of tf-FET devices were fabricated with transparent oxide semiconductors which mechanical flexibility is limited. And, there have been no reports of transparent and flexible all-organic tf-FETs fabricated with organic semiconductor channel, gate dielectric, gate electrode, source/drain electrode, and encapsulation for sensor applications. We present the first demonstration of transparent, flexible all-organic sensor based on multifunctional organic FETs with organic semiconductor channel, gate dielectric, and electrodes having a capability of sensing infrared (IR) radiation and mechanical strain. The key component of our device design is to integrate the poly(vinylidene fluoride-triflouroethylene) (P(VDF-TrFE) co-polymer directly into transparent and flexible OFETs as a multi-functional dielectric layer, which has both piezoelectric and pyroelectric properties. The P(VDF-TrFE) co-polumer gate dielectric has a high sensitivity to the wavelength regime over 800 nm. In particular, wavelength variations of P(VDF-TrFE) molecules coincide with wavelength range of IR radiation from human body (7000 nm ~14000 nm) so that the devices are highly sensitive with IR radiation of human body. Devices were examined by measuring IR light response at different powers. After that, we continued to measure IR response under various bending radius. AC (alternating current) gate biasing method was used to separate the response of direct pyroelectric gate dielectric and other electrical parameters such as mobility, capacitance, and contact resistance. Experiment results demonstrate that the tf-OTFT with high sensitivity to IR radiation can be applied for IR sensors.

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