• Title/Summary/Keyword: Light microscopy

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Performance of Organic light-emitting diode by various surface treatments of indium tin oxide (Indium tin oxide 기판의 표면처리에 따른 유기 발광다이오드의 특성)

  • Kim, Sun-Hyuk;Han, Jeong-Whan
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.39 no.9
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    • pp.1-10
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    • 2002
  • We have done various treatments of indium tin oxide (ITO) surface for organic light-emitting diodes (OLEDs), and investigated the surface states by different surface treatments using atomic force microscopy (AFM) and Auger electron spectroscopy (AES). We have fabricated OLEDs deposited by ultra-high vacuum molecular beam deposition system and studied the characteristics of the OLEDs. We have observed the dramatical improvement of the performance of OLEDs fabricated on ITO substrates treated by $O_2$ plasma treatment reduces the carbon comtamination of ITO surfaces and increases the work function of ITO.

The Oogenesis of Glow-light Tetra, Characidae, Teleost (경골어류 카라신과 glow-light tetra의 난자형성과정)

  • Lee, Kyu-Jae;Chang, Byung-Soo;Teng, Yung-Chien;Kim, Dong-Heui
    • Applied Microscopy
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    • v.38 no.4
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    • pp.315-319
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    • 2008
  • Glow-light tetra, Hemigrammus erythrozonus is a teleost belonging to Characidae. The natural habitate of this fish is the wild in the Essequibo river, Guyana and South America. The oogenesis of glow-light tetra was investigated by light microscopy to compare with those of other families. A pair of ovary was located between swim bladder and intestines. The ovary was of white color and ellipsoidal shape with the major axis 11 mm and the minor axis 4 mm. Cytoplasm of oogonia was basophilic and many nucleoli were located at inside of nuclear membrane. In primary oocytes, yolk vesicles were distributed only in the marginal area and egg envelope was not formed on the outside of an egg. In secondary oocytes, the egg envelope was formed and yolk vesicles in the cytoplasm were increased than the earlier stage. The amount of basophilic substance was decreased. In case of matured egg, thickness of egg envelope and size of egg were increased, basophilic substance was distributed in only around the egg envelope. The yolk vesicles were changed to yolk mass in accordance with development. In conclusion, the oogenesis of glow-light tetra, Hemigrammus erythrozonus was characterized by the increase in cell size, the formation and accumulation of yolk, and the decrease of basophilia a in the cytoplasm. The oogenesis of glow-light tetra seems to share common patterns in Characidae, teleost and have a similar pattern with other teleost.

Measurement of Refractive Index Profile of Optical Fiber Using the Diffraction Phase Microscope (회절위상현미경을 이용한 광섬유의 굴절률 프로파일 측정)

  • Jafar-Fard, Mohammad R.;Moon, Sucbei
    • Korean Journal of Optics and Photonics
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    • v.23 no.4
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    • pp.135-142
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    • 2012
  • We have developed a measurement method of the refractive index profile of an optical fiber by using diffraction phase microscopy. In the microscope system, the reference light was extracted directly from the probe light that passed through the sample by means of pinhole filtering with a diffraction grating. The spatial interference pattern produced by the probe light and the reference light was processed to generate the phase image of the sample fiber. The index profile was obtained by the inverse Abel transform of the phase profile. In order to remove the background phase that originated from the index difference between the cladding and the surrounding medium, the background phase was calculated from the phase data of the cladding to make a core phase profile that can be directly transformed to the index profile of the core without the full phase image that includes the entire cladding part.

Improvement in Light Extraction Efficiency of 380 nm UV-LED Using Nano-patterned n-type Gan Substrate (나노 구조의 패턴을 갖는 n-type GaN 기판을 이용한 380 nm UV-LED의 광 추출 효율 개선)

  • Baek, Kwang-Sun;Jo, Min-Sung;Lee, Young-Gon;Sadasivam, Karthikeyan Giri;Song, Young-Ho;Kim, Seung-Hwan;Kim, Jae-Kwan;Jeon, Seong-Ran;Lee, June-Key
    • Korean Journal of Materials Research
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    • v.21 no.5
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    • pp.273-276
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    • 2011
  • Ultraviolet (UV) light emitting diodes (LEDs) were grown on a patterned n-type GaN substrate (PNS) with 200 nm silicon-di-oxide (SiO2) nano pattern diameter to improve the light output efficiency of the diodes. Wet etched self assembled indium tin oxide (ITO) nano clusters serve as a dry etching mask for converting the SiO2 layer grown on the n-GaN template into SiO2 nano patterns by inductively coupled plasma etching. PNS is obtained by n-GaN regrowth on the SiO2 nano patterns and UV-LEDs were fabricated using PNS as a template. Two UV-LEDs, a reference LED without PNS and a 200 nm PNS UV-LEDs were fabricated. Scanning Electron microscopy (SEM), Transmission Electron Microscopy (TEM), X-Ray Diffraction (XRD), Photoluminescence (PL) and Light output intensity- Input current- Voltage (L-I-V) characteristics were used to evaluate the ITO-$SiO_2$ nanopattern surface morphology, threading dislocation propagation, PNS crystalline property, PNS optical property and UVLED device performance respectively. The light out put intensity was enhanced by 1.6times@100mA for the LED grown on PNS compared to the reference LED with out PNS.

Biological Applications of White Light Scanning Interferometry (백색광 주사간섭계의 생물학적 응용)

  • Kim, Ki-Woo
    • Applied Microscopy
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    • v.41 no.4
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    • pp.223-228
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    • 2011
  • White light scanning interferometry has been employed to analyze surface features of diverse specimens. Long established in the field of materials engineering, the technique provides quantitative three-dimensional data as well as qualitative morphological images. It uses white light that is split and reflected from a reference mirror and an object. Merged together, the light generates interference patterns representing topographical contours of the object surface. The amplitude of the z-axis data is differentiated by gray scale. The technique allows the rapid, noncontact, and wide-field measurements for morphometry of biological specimens including chondrocytes, tooth enamel, and plant leaves. Quantification of the dimension of surface structures such as width, length, and elevation angle could be achievable by white light scanning interferometry. The light reflection from plant leaves has been assumed to be sufficient for the technique. Without special specimen preparations like conductive metal coating, the technique can be increasingly used for quantitative three-dimensional surface measurements of biological specimens.

Ultrastructural Study of Rat Skin Tumor Induced by 7, 12-Dimethylbenz[a]anthracene(DMBA) (7, 12-Dimethylbenz[a]anthracene(DMBA)에 의해 유발된 백서 피부종양의 미세구조적 연구)

  • Deung, Young-Kun;Kim, Wan-Jong;Park, Kwang-Kyun
    • Applied Microscopy
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    • v.20 no.2
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    • pp.102-116
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    • 1990
  • To investigate the ultrastructural features of rat back skin tumor induced by 7, 12-Dimethylbenz[a]anthracene(DMBA), morphological changes during carcinogenesis were undertaken by the light and the electron microscopes. On the light microscopic levels, dysplasia was observed at the 6th week of treatment with 0.5% DMBA, hyperplasia at the 8th week, and carcinoma in sitv or invasive carcinoma at the 12th week. In addition to those characteristics, papillomatosis was observed at the 18th week. Under the electron microscopy, intercellular spaces were enlarged in the early phase. Nucleolar enlargement, the increase of free ribosomes, the condensation of tonofibrils, and the irregular arrangement of desmosomes appeared at the 6th week of application. Thereafter, the degradation of desmosomes and the discontinuity of basal lamina were characterized. And then, epidermal cell processes were protruded toward the connective tissue at the site of discontinuity of basal lamina at the 12th week. At the 14th to 18th week, DMBA-induced fibrosarcomas or papillomas were observed.

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Fabrication of Micro-Lens Array with Long Focal Length for Confocal Microscopy (공초점 현미경용 장초점 마이크로렌즈 제작)

  • Kim, Gee-Hong;Lim, Hyung-Jun;Jeong, Mi-Ra;Lee, Jae-Jong;Choi, Kee-Bong;Lee, Hyung-Seok;Do, Lee-Mi
    • Journal of the Korean Society of Manufacturing Technology Engineers
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    • v.20 no.4
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    • pp.472-477
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    • 2011
  • This paper shows the method of fabrication of a micro lens array comprised of a Nipkow disk used in a large-area, high-speed confocal microscopy. A Nipkow disk has two components, a micro lens array disk and a pinhole array disk. The microlens array focuses illumination light onto the pinhole array disk and redirects reflected light from a surface to a sensor. The micro lens which are positioned in order on a disk have a hemispheric shape with a few tens of micron in diameter, and can be fabricated by a variety of methods like mechanical machining, semiconductor process, replication process like imprinting process. This paper shows how to fabricate the micro lens array which has a long focal length by reflow and imprinting process.

Microscopic Patterns of Decay caused by Tyromyces palustris and Gloeophyllum trabeum in Korean Red Pine and Radiata Pine Woods

  • Kim, Hwa Sung;Eom, Young Geun
    • Journal of the Korean Wood Science and Technology
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    • v.34 no.5
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    • pp.1-10
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    • 2006
  • The objective of this study was to elucidate the microscopic patterns of decay caused by brown-rot fungi of Tyromyces palustri and Gloeophyllum trabeum in Korean red pine (Pinus densiflora) and radiata pine (Pinus radiata) woods through light and electron microscopies. The ultrastructural changes of cell walls attacked by the two brown-rot fungi were compared in this respect. Macroscopically, radiata pine showed more ring and radial checks than Korean red pine. Microscopically, with the progress of decay, spiral checks associated with cross-field pits and bore holes in the cell wall were more remarkably numerous in the radiata pine than in the Korean red pine. In the radiata pine, G. trabeum produced more spiral checks in the cell wall than T. palustris. In the advanced stages of decay by G. trabeum, the erosions of ray cell walls were identified both in the Korean red pine and radiata pine but $S_3$ layers of tracheid walls were eroded only in the Korean red pine.

Light and Electron Microscopic Characterization of Husk from Korean Rice

  • Adya P. Singh;Park, Byung-Dae;Wi, Seung-Gon;Lee, Kwang-Ho;Yoon, Tae-Ho;Kim, Yoon-Soo
    • Plant Resources
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    • v.5 no.2
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    • pp.95-103
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    • 2002
  • Microscopic techniques were used to observe the microstructure of rice husk. Microscopic examination showed that two main components of husk, lemma and palea consisted of outer epidermis, layers of fibers, vascular bundles, parenchyma cells, and inner epidermis, in sequence from the outer to the inner surface. The outer epidermal walls were extremely thick, highly convoluted and lignified. The underlying fibers were also thick-walled and lignified. Parenchyma cells were thin-walled and unlignified. Inner epidermal cells were also unlignified. The outer surface of both lemma and palea were conspicuously ridged, but the lower surface had a flat appearance. As part of a detailed study to characterize rice husk using microscopic and micro-analytical techniques, distribution of silica was also examined, and is presented elsewhere. Rice husk can potentially be used as a raw material for making composite products and the observations presented here form valuable background information for our future work related to product development.

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Conceptual Design of Laser Plasma-based Soft X-ray Microscope system for Biomedical Application (레이저 플라즈마 기반의 생물의료용 연 X-선 현미경 설계)

  • 김경우;윤권하
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2003.06a
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    • pp.690-693
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    • 2003
  • Soft x-ray microscopy provides a unique set of capabilities in-between those of visible light and electron microscopy. It has long been recognized that nature provides a 'water window' spectral region between the K shell x-ray absorption edges of carbon (~290eV) and oxygen (~540eV), where organic materials show strong absorption and phase contrast, while water is relatively non-absorbing. This enables imaging of hydrated biological specimens that are several microns thick with high intrinsic contrast using x-rays with a wavelength of 2.3~4.4nm. Soft X-ray microscopy is therefore well suited to the study of specimens like single biological cells. The most direct advantage of X-ray microscope is their high spatial resolution when compared with visible light microscopes, combined with an ability to image hydrated specimens that are several microns with a minimum of preparation. Our study describes the conceptual design of soft x-ray microscope system based on a laser-based source for biomedical application with high resolution ($\leq$50nm) and short exposure time ($\leq$30sec).

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