• Journal of Fisheries and Marine Sciences Education
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• v.29 no.2
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• pp.552-559
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• 2017

To assess the effect of LED lights on marine microalgae growth in the laboatory, Tetraselmis suecica, Chaetoceros simplex and Isochrysis galbana were cultured under $20{\pm}1^{\circ}C$, Walne's medium and aeration using 3.6 L glass vessels. The LED light sources were Blue, Red, Blue+Red, CoolWhite and WarmWhite. The experiments were conducted three times. The density of microalgae was shown as the counted number of cells per day, and the specific growth rate was calculated by using the density. The statistical analysis was performed by analysis of variance using the SPSS 20.0 program. T. suecica culture was the highest density under the Blue LED light source, so the light source was the most effective for the growth of this alga. C. simplex and I. galbana culture had the highest density under the Blue+Red LED light source, therefore this light source was the most effective for the growth of these algae. The result of analysis of variance showed significant between groups.

• KSBB Journal
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• v.14 no.2
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• pp.160-166
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• 1999

High cell density cultivation of microalga Dunaliella bardawil using nitrogen fed-batch cultures was studied in batch flask. Optimum environmental conditions include concentrated nutrients except NaCl and carbon sources, carbon sources, pH, light, agitation, nitrate and phosphate ions. Cell growth, consumption rates of nitrate and phosphate ions were monitored. Optimal conditions for higher cell density were found to be(in the range tested): 5 times concentrated media(1 times-10 times concentrated media) pH 8.0 (7.0-9.0) white light(blue and red light) 15mM of nitrate (0.94-15mM) 250mM $NaHCO_3$ and $CO_2$ gas. However, the addition of phosphate ions did not enhance the algal maximum cell density and specific growth rate. Nitrate was found to be effective for the cell growth. The maximum cell density of fed-batch culture using nitrate ions in $8.955{\times}106$cells/ml after 189hr incubation.

• Journal of Biosystems Engineering
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• v.24 no.1
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• pp.19-24
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• 1999

Motivated by the need for developing the more efficient lighting system for light culture of strawberries in the greenhouse, this paper aims at acquiring and suggesting more concrete and scientific foundation of illuminating position, numbers of light source by investigating the types of lighting system and illumination distribution in the greenhouse for light culture of strawberries. The results of investigation and experiment are summarized as following below: 1. The types of lighting system used in the greenhouse producing strawberries were classified as 1 line and 2 lines lighting system. 2. As for the arranging types of artificial light, 2 lines lighting system, were classified as Z-type, N-type and W-type. (Refer Fig. 3) 3. The results of illumination distribution for Z-type, N-type and W-type of 2 lines illuminating system in the greenhouse with a small size tunnel measured at the height of 1.5m from the ground with 220V, 100W lamp in 6m light gap showed that maximum illuminance are 961x, 1211x, 1251x, minimum illuminance are 4.41x, 4.71x, average illuminance are 33.71x, 43.11x, 44.51x and standard deviations are 28.31x, 35.41x, 38.31x at each types. 4. Proportion of the area below optimal illuminance to floor area at the two lines illuminating system of Z-, N-, and W-type in greenhouse were appeared as 39.4%, 26.0% and 26.3%, respectively. Also proportion of the area over optimal illuminance to floor area at the two lines illuminating system of Z-, N-, W-type in greenhouse were appeared as 16.8%, 14% and 14.7%, respectively. Thus N-type was superior to the others from the view points of optimal illumination distribution and energy saving.

• Proceedings of the Costume Culture Conference
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• 2000.09a
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• pp.46-48
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• 2000

• Proceedings of the Costume Culture Conference
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• 2003.02a
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• pp.26-27
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• 2003

• Journal of Biosystems Engineering
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• v.32 no.2 s.121
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• pp.109-114
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• 2007

In mass production of seed-potato plantlets, the processes for in vitro propagation and ex vitro acclimatization with a high cost should be improved by a culture system with environmental control using scaled-up culture vessels. The experiment was conducted to design a hydroponic culture system for enhancement of growth and development of seed-potato (Solanum tuberosum) plantlets cultured under photoautotrophic (without sugar in culture medium) conditions with controlled light intensity and ventilation rate. The culture system was consisted of scaled-up culture vessels, ventilation pipes, a multi-cell tray and an environmental control system (ECS) for optimum controlling in temperature, light intensity, ventilation rate, and culture-medium supply. Growth and development of the plantlets was significantly increased under the ECS compared with a conventional culture system (CCS) of photomixotrophic culture (with sugar in culture medium) using small scale vessels. For 21 days, leaf area of the plantlets was expanded more than 2 times, and number of internodes also approximately 4 times greate. under the ECS. In addition, the photoautotrophic growth in sweetpotato (Ipomoea batatas) and chrysanthemum (Chrysanthemum morifolium) plantlets was greater more than 2 times compared with the CCS.

• ALGAE
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• v.30 no.1
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• pp.35-47
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• 2015

The marine sand-dwelling dinoflagellate Polykrikos lebourae possesses obvious gold-brown pigmented plastids as well as taeniocyst-nematocyst complex structures. Despite of the presence of the visible plastids, previous attempts to establish this species in culture all failed and thus the unavailability of cultures of this species has posed a major obstacle to further detailed exploration of ecophysiology of the dinoflagellate. Here, we isolated P. lebourae from sandy sediment of an intertidal flat on Korean western coast, successfully established it in culture, and have been maintaining the stock culture over the past 3 years. Using this stock culture, we explored phagotrophy and potential prey resources of P. lebourae, growth and grazing responses of P. lebourae to different prey organisms, the effect of prey concentration on growth and grazing rates and gross growth efficiency (GGE) of P. lebourae when fed three different prey organisms, and the growth kinetics of P. lebourae under different light regimes. P. lebourae captured prey cells using a tow filament and then phagocytized them through the posterior end. The dinoflagellate was capable of ingesting a broad range of prey species varying in size, but not all prey species tested in this study supported its sustained growth. GGE of P. lebourae was extremely high at low prey concentration and moderate or low at high prey concentrations, indicating that P. lebourae grows heterotrophically at high prey concentrations but its growth seems to be more dependent on a certain growth factor or photosynthesis of plastids derived from the prey. In the presence of prey in excess, P. lebourae grew well at moderate light intensity of $40{\mu}mol$ photons $m^{-2}s^{-1}$, but did not grow at dim and high (10 or $120{\mu}mol$ photons $m^{-2}s^{-1}$) light intensities. Our results suggest that the benthic dinoflagellate P. lebourae is an obligate mixotroph, requiring both prey and light for sustained growth and survival.

• Journal of Ginseng Research
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• v.41 no.3
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• pp.370-372
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• 2017

Three greenhouse experiments with American ginseng seedlings growing under light levels from 4.8% to 68% showed a quadratic response for root dry weight, giving an optimal root dry weight of 239 mg (range 160-415 mg) at an optimal light level of 35.6% (range 30.6-43.2%).

• International Journal of Advanced Culture Technology
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• v.8 no.3
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• pp.260-268
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• 2020

Currently, it is usually a 3D scanner processing method as a laser method. However, the laser method has a disadvantage of slow scanning speed and poor precision. Although optical scanners are used as a method to compensate for these shortcomings, optical scanners are closely related to the distance and precision of the object, and have the disadvantage of being expensive. In this paper, 3D scanner using variable focus lens-based structured light module with improved measurement precision was designed to be high performance, low price, and usable in industrial fields. To this end, designed a telecentric optical system based on a variable focus lens and connected to the telecentric mechanism of the step motor and lens to adjust the focus of the variable lens. Designed a connection structure with optimized scalability of hardware circuits that configures a stepper motor to form a system with a built-in processor. In addition, by applying an algorithm that can simultaneously acquire high-resolution texture image and depth information and apply image synthesis technology and GPU-based high-speed structured light processing technology, it is also stable for changes to external light. We will designed and implemented for further improving high measurement precision.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.51 no.1
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• pp.64-71
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• 2018

This study was conducted to investigate the effects of light intensity and wavelength on the growth of Tetraselmis suecica and Tetraselmis tetrathele. These species were exposed to a blue light-emitting diode (LED; max=450 nm), a yellow LED (max=590 nm), a red LED (max=630 nm) and a fluorescent lamp (three wavelengths). The maximum growth rates (${\mu}_{max}$) of T. suecica and T. tetrathele under a red LED were 1.12/day and 0.95/day, respectively. Under a yellow LED, growth rates were 70% of the values for red wavelength, with low half-saturation constants (Ks). The optimum light source to ensure economically effective and productive growth in a Tetraselmis culture system (Photo-Bioreactor) would thus appear to be a three-phase culture, wherein a yellow LED is used during the lag phase and initial exponential phase to increase growth rate, followed by a red LED during the middle exponential phase to maximize growth rate, and finally a yellow LED again during the late exponential phase and stationary phase to achieve increased yield of useful bioactive substances.