• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2000년도 춘계수산관련학회 공동학술대회발표요지집
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• pp.446-446
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• 2000

Specific polyclonal and/or monoclonal antibodies (MAbs) to immunoglobulins (Igs) and their subunits have proved to be valuable tools in immunological research and in immunological assays. In this study, we developed and characterized MAbs against flounder serum Igs. To obtain the pure flounder serum Igs, mouse IgG (mIgG) was immunized to flounder. Flounder Igs were purified by using mIgG-agarose affinity column chromatography. The structure of purified flounder Ig was observed, on denatured SDS-PAGE, to be composed of two heavy chains (77 and 72 kd) and two light chains (28 and 26 kd). MAbs were produced by fusion of myeloma cells (SP2/0) with Balb/c mouse spleen cells previously primed with the flounder Igs. Finally, three hybridoma clones, FIM 511, FIM 519 and FIM 562 were established to recognize both 2 heavy chains, 26 kd of light chain and 28 kd of light chain, respectively. On the other hand, the flounder immune sera collected on the weekly basis were tested on ELISA and immunoblot analysis whether boosting effect is present in flounder humoral immune system. As a result, the secondary immune response in flounder was ascertained on ELISA, but not on immunoblot analysis. Further, we observed an alteration of serum protein levels following immunization. Our MAbs and basic information on flounder humoral immune system obtained in this study will be helpful to control and monitor the efficiency of fish vaccines and therapeutic process of flounder diseases.

• Carbon letters
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• 제13권1호
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• pp.34-38
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• 2012

Successful application of graphene requires development of various tools for its chemical modification. In this paper, we present a Raman spectroscopic investigation of the effects of UV light on single layer graphene with and without the presence of $O_2$ molecules. The UV emission from a low pressure Hg lamp photolyzes $O_2$ molecules into O atoms, which are known to form epoxy on the basal plane of graphene. The resulting surface epoxy groups were identified by the disorder-related Raman D band. It was also found that adhesive residues present in the graphene samples prepared by micro-mechanical exfoliation using adhesive tape severely interfere with the O atom reaction with graphene. The UV-induced reaction was also successfully applied to chemical vapor deposition-grown graphene. Since the current method can be readily carried out in ambient air only with UV light, it will be useful in modifying the surfaces of graphene and related materials.

• 한국광학회지
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• 제24권6호
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• pp.338-341
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• 2013

본 논문에서는 혈액의 응고 시간을 나타내는 잣대로 활용되는 PT (prothrombin time)를 반사광을 이용하여 측정하는 방법에 대한 광학적 분석이 기술된다. 혈액에 일정량의 thromboplastin을 정량 추가하면 혈액의 응고 과정이 진행된다. 응고 시간을 측정하기 위하여 혈액에 자성 철 가루를 혼입하고 자석을 회전시키면서 혈액 표면을 레이저로 조사하여 그 반사광의 변화를 측정하였다. 이 신호를 분석하면 표준 값에 대응하는 PT 값을 환산할 수 있었다. 측정된 반사광 신호를 전산 모델링으로 나타내어 측정 원리를 분석한 결과도 같이 제시한다.

• 한국광학회지
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• 제18권4호
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• pp.264-269
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• 2007

기존 필라멘트 방식의 루미나리에용 조명의 단점을 극복하기 위해 필라멘트를 LED로 바꾸기 위한 연구가 활발하게 진행 중이다. 본 연구는 루미나리에용에 적합한 LED패키지 구조를 만들기 위해 ray tracing simulator를 이용해 다양한 구조를 모델링하고 시뮬레이션을 통해 각 구조에서의 배광분포를 측정하였다. 시뮬레이션 결과, LED 패키지의 내부 반사경의 각을 증가시킬수록 배광분포가 두 개의 피크로 갈라지면서 피크간의 각이 점점 커졌다. 또한 외부 반사경 각이 증가함에 따라 양쪽 피크간의 각이 점점 줄어들다가 $50^{\circ}$를 기준으로 다시 증가하였다. 이러한 결과는 LED에서 발생한 빛이 두 개의 반사경에 의하여 반사되는 각도가 달라짐에 의하여 발생함을 알 수 있었다. Ray tracing simulator를 이용하여 구한 LED 패키지 구조의 조건을 적용하여 루미나리에용 LED패키지를 제작하였고 배광분포 측정기를 이용해 광 특성을 측정하였으며 시뮬레이션의 결과와 유사한 특성을 얻었다.

• International Journal of Internet, Broadcasting and Communication
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• 제12권4호
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• pp.128-136
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• 2020

In this paper, we presented a simple hologram recording set for learners to broaden their program choices and experience holographic techniques with lifelong education programs. In order to obtain quality 3D images, the quality of light sources and recording mediums, which are the main elements of hologram recording, must be good. In addition, due to the characteristics of the hologram, the ambient vibrations shall be minimized for accurate interference pattern recording. The simple hologram recording set presented in this paper has not significantly restricted in space and location, and anyone can easily experience it at a reasonable price compared to the existing hologram production method. Also, it can make two different type of hologram (transmission and reflection) for providing easy access to recording and reconstruction of the hologram. Experiments have shown that holographic manufacturing practices are a very useful way to educate the public on optics and photonics technologies.

• 한국지능시스템학회논문지
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• 제23권1호
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• pp.24-28
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• 2013

이 논문에서는 아동 크기 휴머노이드 로봇의 설계 및 개발과정에 대하여 기술한다. 경량형 휴머노이드 로봇의 설계 개념을 제시하고, 3차원 설계 툴을 이용하여 1m 이상 크기 휴머노이드 로봇의 메커니즘을 설계하였다. 로봇의 구동을 위한 하드웨어는 제한된 로봇의 무게 내에서 최적의 성능을 낼 수 있도록 설계하였다. 3차원 설계 툴을 사용하여 설계한 로봇의 프레임 및 링크들은 가볍고 강도가 좋은 재료를 선정하고 정밀 가공을 통해 제작하였다. 제작된 아동 크기의 휴머노이드 로봇은 역기구학, 균형제어를 적용하여 기본 동작을 구현하고 그 성능을 실험을 통하여 확인하였다.

• 대한기계학회논문집
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• 제17권2호
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• pp.351-358
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• 1993

Recently a high speed spindle system for machine tools has attracted considerable attention to reduce the machining time, to improve the machining accuracy, to perform the machining of light metals and hard materials and to unite the cutting and grinding processes. In this study, a high speed spindle system is developed by applying the oil-air lubrication method, angular contact ball bearings, injection nozzles with dual orifices and so on. And a lubrication experiment for evaluating the performance of the spindle system is carried out. Especially, in order to establish the lubrication conditions related to the development of a high speed spindle system, the effects of oil supply rate, rotational spindle speed and so on are studied and discussed on the bearing temperature rise, bearing temperature distribution and frictional torque. And the effect of spindle system structure on the bearing temperature distribution is investigated.

• 한국정밀공학회지
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• 제31권3호
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• pp.189-197
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• 2014

Diffractive optical elements (DOEs) - in general a complex pattern of micro- and nano-scale structures - can modulate and transform light in a predetermined way. Their importance is being increased nowadays because they can be designed to handle a number of simultaneous tasks. In view point of machining DOEs, it is a big challenge to fabricate micro- and nano-scale structures on a free-form surfaces. In this paper, the state-of-the-art of the ultra-precision machine tools is reviewed. Also some technical issues which determine the machine tool accuracy are discussed.

• 식품과학과 산업
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• 제50권1호
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• pp.2-10
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• 2017

Human have eaten various traditional fermented foods for a numbers of million years for health benefit as well as survival. The beneficial effects of fermented foods have been resulted from complex microbial communications within the fermented foods. Therefore, the holistic approaches for individual identification and complete microbial profiling involved in their communications have been of interest to food microbiology fields. Microbiome is the ecological community of microorganisms that literally share our environments including foods as well as human body. However, due to the limitation of culture-dependent methods such as simple isolations of just culturable microorganisms, the culture-independent methods have been consistently developed, resulting in new light on the diverse non-culturable and hitherto unknown microorganisms, and even microbial communities in the fermented foods. For the culture-independent approaches, the food microbiome has been deciphered by employing various molecular analysis tools such as fluorescence in situ hybridization, quantitative PCR, and denaturing gradient gel-electrophoresis. More recently, next-generation-sequencing (NGS) platform-based microbiome analysis has been of interest, because NGS is a powerful analytical tool capable of resolving the microbiome in respect to community structures, dynamics, and activities. In this overview, the development status of analysis tools for the fermented food microbiome is covered and research trend for NGS-based food microbiome analysis is also discussed.

• 한국정밀공학회지
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• 제11권1호
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• pp.123-128
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• 1994

A high speed spindle system for machine tools can be used to reduce the machining time, to improve the machining accuracy, to perform the machining of light metals and hard materials, and to unite the cutting and grinding processes. In this study, a high speed spindle system is developed by applying the oil-air lubrication method, angular contact ball bearings, injection nozzles with dual orifices, cooling jacket and so on. And an air cooling experiment for evaluating the performance of the spindle system is carried out. Especially, in ofder to establish the air cooling conditions related to the development of a high speed spindle system, the effects of cooling air pressure, oil supply rate, air supply rate and rotational spindle speed are studied and discussed on the bearing temperature rise and frictional torque. Also the effects of cooling air pressure, rotational spindle speed and spindle system structure is investigated on the bearing temperature distribution. The experiment on the test model reveals the usefulness of the air cooling method.