• Proceedings of the Korean Society of Crop Science Conference
• /
• 2018.10a
• /
• pp.71-71
• /
• 2018

• IMMUNE NETWORK
• /
• v.9 no.6
• /
• pp.243-247
• /
• 2009

In this study, we report the development of a new dual reporter vector system for the analysis of promoter activity. This system employs green fluorescence emitting protein, EGFP, as a reporter, and uses red fluorescence emitting protein, DsRed, as a transfection control in a single vector. The expression of those two proteins can be readily detected via flow cytometry in a single analysis, with no need for any further manipulation after transfection. As this system allows for the simultaneous detection of both the control and reporter proteins in the same cells, only transfected cells which express the control protein, DsRed, can be subjected to promoter activity analysis, via the gating out of all un-transfected cells. This results in a dramatic increase in the promoter activity detection sensitivity. This novel reporter vector system should prove to be a simple and efficient method for the analysis of promoter activity.

• Journal of Applied Reliability
• /
• v.5 no.2
• /
• pp.273-287
• /
• 2005

Accelerated life test models and procedure are developed to assess the reliability of Refrigerator door switch. The main function of door switch is to operate bulb lamp and fan motor in the refrigerating room. The accelerated life test method and test equipments are developed using the relationship between stresses and life characteristics of the products. Using the developed accelerated life test method, the parameters of the ALT model and life time distribution are estimated and the reliability of the Door S/W at use condition if assessed. The proposed accelerated life test method and procedure may be extended and applied to testing similar kinds of products to reduce test time and costs of the tests remarkably.

• Journal of Families and Better Life
• /
• v.8 no.2
• /
• pp.101-118
• /
• 1990

The purpose of this study is to examine the stress level and factors percepted by the married women during family life, and to identify the related variables influencing on stress level. For this purpose, questionnaires were distributed to the 650 married women who have been living in seoul. Among them 463 data were selected. For data analysis, the statistical method such as the factor analysis frequency distribution percentile , t-test, ANOVA, Duncan's Multiple Range Test and Stepwise Regression Analysis were used. the major findings were summarized as follows; 1) The general tendancy of the stress level percepted by the married women during family life was relatively low. 2) The household background variables(age, level of education, income, type of family, number of children, status of employment ) have turned out to be significant on the stress level of married women except family type. 3) All the social-psychological variables(socio-economic status, communication interaction, resources perception, psychological satisfaction) showed significant differences according to the stress level percepted by the married women during family life. 4) The level of work identity was the important factor on the stress level percepted by the married women during family life. 5) In Stepwise Multiple Regression Analysis, the married women's stress level during family life was greatly influenced by variables such as the resource perception and the psychological satisfaction.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.24 no.10 s.181
• /
• pp.2654-2663
• /
• 2000

Steam turbine rotors are the most critical and highly stressed components of a steam power plant; therefore, the life expectancy of the turbine rotor is an important consideration for the safety of a steam power plant. The objective of this paper is to develop a life estimation program for turbine rotors for all possible operating conditions. For this purpose, finite element analysis was carried out for four normal operating modes (cold, warm, hot and very hot starts) using ABAQUS codes. The results are made into databases to evaluate the life expenditure for an actual operating condition. For any other possible abnormal operating condition, the operating data are transmitted to the server (workstation) through a network to carry out finite element analysis. Damage estimation is carried out by transmitting the finite element analysis results to the personal computer, and then the life expectancy is calculated.

• Korean Journal of Adult Nursing
• /
• v.14 no.3
• /
• pp.401-410
• /
• 2002

Purpose: This research was designed to develop an instrument that can be used for measuring of the quality of life for the elderly in Korea. Method: Subjects were 2,110 aged people, residing in Seoul and Kyeungi-Do. In developing this instrument, the researcher first established a conceptual framework based on the literature review and interview and open questionnaire. The collected data were analyzed using the SPSS-WIN program. For the component factors of the measuring instrument, a factor analysis method and item analysis was used. The reliability of the scale was tested by Cronbach's alpha. Result: 1) The results of the analysis of the 32 items making up the instrument were classified into the following 4 factors: life stability, easy of resource utility, health and affection in family, establishment of supportbase. 2) The reliability of the instrument for the quality of life was 0.952 (Cronbach's alpha). In the factor analysis Cronbach's alpha correlations for the 4 factors ranged from 0.736 to 0.949. Conclusion: This scale could be adequately applied in assessing the quality of life for the elderly in Korea. And the results of this scale in a study can contribute to the promotion of quality of life for the elderly.

• The Korean Journal of Food And Nutrition
• /
• v.20 no.3
• /
• pp.334-340
• /
• 2007

The purpose of this study is to analyze silver consumers' life style, and whether silver consumers ’ life style have an effect on purchase satisfaction and repurchase intention of the health functional foods. To this end, a survey was conducted from May 28 to June 20 in 2007, among silver consumer aged in over 50s, on their purchase experience of the health functional foods. The survey was conducted with 326 subjects, and the statistical analysis methods were frequency analysis, factor analysis, and multiple regression analysis. According to the result of this study, First, the silver consumers' life style factors were determined to be health and leisure pursuit, material pursuit, family pursuit, and outgoing challenge pursuit. And purchase satisfaction factors of the health functional foods were determined to be taking after dosage and service, function and purchase, appearance of health functional foods. Second, silver consumers' family pursuit life style and outgoing challenge life style had an effect on taking and service satisfaction, appearance and products satisfaction of the health functional foods. Third, purchase satisfaction had an effect on repurchase intention of the health functional foods. Therefore, this study is significant in that it clarifies the relation of silver consumers' life style and purchase satisfaction and repurchase intention of the health functional foods.

• Research in Community and Public Health Nursing
• /
• v.29 no.4
• /
• pp.510-519
• /
• 2018

Purpose: The purpose of this meta-analysis isto identify social psychological factors related to quality of life and estimate the effect sizes of the factors among patients with strokes. Methods: Thirteen studies with a total of 1,814 patients published from the earliest records to January 8, 2017 were selected through a systematic process of searching the literature, and evaluated against influencing factors of quality of life and their effect sizes. Pooled effect sizes were calculated using the random effect model. Meta-analysis was conducted by R software. Results: The following influencing factors had a strong association with quality of life with stroke: depression (r=-.50; 95% CI: -0.63~-0.46), activities of daily living (r=.46; 95% CI: 0.35~0.56), and social support (r=.40; 95% CI: 0.24~0.53). Conclusion: The findings confirm that depression, activities of daily living and social support are associated with quality of life among patients with stroke survivors. We recommend that any intervention program to improve the quality of life with stroke patients consider addressing these modifiable influencing factors.

• Transactions of the Korean Society of Machine Tool Engineers
• /
• v.13 no.6
• /
• pp.94-101
• /
• 2004

The purpose of this raper is to assess the benefits of frequency domain fatigue analysis and compare it with more conventional time domain techniques. The multi-body dynamic analysis, FE analysis and fatigue life prediction technique are applied for the frequency domain fatigue analysis. To obtain the dynamic load history used in the frequency domain fatigue analysis, the computer simulations running over typical road Profiles are carried out by utilizing vehicle dynamic model. The fatigue life estimation for the rear suspension system of small-sized passenger car is performed by using resonance durability analysis technique, and the estimation results are compared with the conventional quasi-static durability analysis results. For the pothole simulation, the percent changes, of the fatigue life between the two durability analysis techniques don't exceed 10%. But for the Belgian road simulation because of the resonance effect, the fatigue life using the resonance durability analysis technique are much smaller estimated than the quasi-static durability analysis results.

• Transactions of Materials Processing
• /
• v.24 no.4
• /
• pp.264-271
• /
• 2015

In the current study, a method was proposed to quantitatively predict the wear and fatigue life of a shearing die in order to determine an effective replacement period for the die. The shearing die model of a retainer manufacturing process was used for the proposed method of quantitative life prediction. The retainer is produced through shearing steps, such as piercing and notching. The shearing die of the retainer is carefully controlled because the dimensional accuracy of the retainer is critical. The fatigue life for the shearing die was predicted using ANSYS considering S-N curves of STD11 and Gerber’s equation. The wear life for the shearing die was predicted using DEFORM-3D considering the Archard’s wear model. Experimental shearing of the retainer was conducted to verify the effectiveness of the proposed method for predicting die life. The fatigue failure of the shearing die was macroscopically measured. The wear depth was measured using a 3D coordinate measuring machine. The results showed that the wear and fatigue life in the FE analysis agree well with the experimental results.