• Asian Pacific Journal of Cancer Prevention
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• 제13권7호
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• pp.3223-3228
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• 2012

HMGN5 is a typical member of the HMGN (high mobility group nucleosome-binding protein) family which may function as a nucleosomal binding and transcriptional activating protein. Overexpression of HMGN5 has been observed in several human tumors but its role in tumorigenesis has not been fully clarified. To investigate its significance for human lung cancer progression, we successfully constructed a shRNA expression lentiviral vector in which sense and antisense sequences targeting the human HMGN5 were linked with a 9-nucleotide loop. Inhibitory effects of siRNA on endogenous HMGN5 gene expression and protein synthesis were demonstrated via real-time RT-PCR and western blotting. We found HMGN5 silencing to significantly inhibit A549 and H1299 cell proliferation assessed by MTT, BrdU incorporation and colony formation assays. Furthermore, flow cytometry analysis showed that specific knockdown of HMGN5 slowed down the cell cycle at the G0/G1 phase and decreased the populations of A549 and H1299 cells at the S and G2/M phases. Taken together, these results suggest that HMGN5 is directly involved in regulation cell proliferation in A549 and H1299 cells by influencing signaling pathways involved in cell cycle progression. Thus, our finding suggests that targeting HMGN5 may be an effective strategy for human lung cancer treatment.

• Asian Pacific Journal of Cancer Prevention
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• 제15권22호
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• pp.9853-9857
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• 2014

Background: The morbidity and mortality rate of liver cancer continues to rise in China and advanced cases respond poorly to chemotherapy. Ribosomal protein L24 has been reported to be a potential therapeutic target whose depletion or acetylation inhibits polysome assembly and cell growth of cancer. Materials and Methods: Total RNA of cultured amycin-resistant and susceptible HepG2 cells was isolated, and real time quantitative RT-PCR were used to indicate differences between amycin-resistant and susceptible strains of HepG2 cells. Viability assays were used to determine amycin resistance in RPL24 transfected and control vector and null-transfected HepG2 cell lines. Results: The ribosomal protein L24 transcription level was 7.7 times higher in the drug-resistant HepG2 cells as compared to susceptible cells on quantitative RT-PCR analysis. This was associated with enhanced drug resistance as determined by methyl tritiated thymidine (3H-TdR) incorporation. Conclusions: The ribosomal protein L24 gene may have effects on drug resistance mechanisms in hepatocellular carcinoma HepG2 cells.

• Journal of Electrical Engineering and Technology
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• 제10권3호
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• pp.865-876
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• 2015

Voltage stability is one of the most critical security issues which has not yet been well resolved to date. In this paper, an analytical method called PQ plane analysis with consideration of the reactive power capability of wind turbine generator and the wake effect of wind farm is proposed for voltage stability study. Two voltage stability indices based on the proposed PQ plane analysis method incorporating the uncertainties of load-increasing direction and wind generation are designed and implemented. Cases studies are conducted to investigate the impacts of wind power incorporation with different control modes. Simulation results demonstrate that the constant voltage control based on reactive power capability significantly enhances voltage stability in comparison of the conventional constant power factor control. Some meaningful conclusions are obtained.

• 한국통신학회논문지
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• 제33권9C호
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• pp.676-682
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• 2008

정보화시대에 진입하면서 디지털 콘텐츠 활용기술이 사회 각 분야에서 각광을 받고 있으며 이와 더불어 효율적인 콘텐츠 관리와 콘텐츠 저작권보호를 위한 관련 기술연구가 활발하게 이루어지고 있다. 그러나 기존 저작권보호를 위한 DRM기술은 단일 CMS 도메인을 기반으로 개발되고 콘텐츠 클로벌 활용이 제한되어 정보의 교류와 공유를 효율적으로 지원하지 못하는 단점이 있다. 이 논문에서는 CMS간 콘텐츠 상호운영이 가능한 계층적 통합 메타데이터를 기반으로 콘텐츠 저작권보호를 위한 DRM모델을 제안한다. 제안 모델은 동종 및 이종 CMS간 콘텐트들이 상호유통이 가능하고 또한 유통과정에서 발생되는 저작권문제를 해결하였다.

• International Journal of Concrete Structures and Materials
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• 제11권1호
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• pp.99-113
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• 2017

The effects of different lightweight functional fillers on the properties of cement-based composites are investigated in this study. The fillers include fly ash cenospheres (FACs) and glass micro-spheres (GMS15 and GMS38) in various proportions. The developed composites were tested for compressive, flexural and tensile strengths at 10 and 28-day ages. The results indicated that both FACs and GMS38 are excellent candidates for producing strong lightweight composites. However, incorporation of GMS15 resulted in much lower specific strength values (only up to $13.64kPa/kg\;m^3$) due to its thinner shell thickness and lower isostatic crushing strength value (2.07 MPa). Microstructural analyses further revealed that GMS38 and GMS15 were better suited for thermal insulating applications. However, higher weight fraction of the fillers in composites leads to increased porosity which might be detrimental to their strength development.

• Carbon letters
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• 제23권
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• pp.9-16
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• 2017

This paper introduces a nitrogen-doped ordered mesoporous carbon (NOMC) derived from glucosamine with hybrid capacitive behaviors, achieved by successfully combining electrical double-layer capacitance with pseudo-capacitance behaviors. The nitrogen doping content of the fabricated NOMC reached 7.4 at% while its specific surface area ($S_{BET}$) and total pore volume reached $778m^2g^{-1}$ and $1.17cm^3g^{-1}$, respectively. A dual mesoporous structure with small mesopores centered at 3.6 nm and large mesopores centered at 9.9 nm was observed. The specific capacitance of the reported materials reached up to $328Fg^{-1}$, which was 2.1 times higher than that of pristine CMK-3. The capacitance retention rate was found to be higher than 87.9% after 1000 charge/discharge cycles. The supplementary pseudocapacitance as well as the enhanced wettability and conductivity due to the incorporation of nitrogen heteroatoms within the carbon matrixes were found to be responsible for the excellent capacitive performance of the reported NOMC materials.

• 한국세라믹학회지
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• 제34권7호
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• pp.722-730
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• 1997

The nonstoichiometry (x) of UO2+x and (Er0.06U0.94)O2+x has been in-situ measure against oxygen activity (Po2) at elevated temperatures by a coulometric titration method. From the dependence of the oxygen excess (x) of UO2+x on Po2 at 1000℃, it has been concluded that (2Vo2Oia2Oib)〃〃and (2Vo2Oia2Oib)' clusters are prevailing at low oxygen partial pressure [log(Po2/atm) -10.6] and at high oxygen partial pressure [log(Po2/atm) -10.6], respectively. The nonstoichiometry is found to be reduced with the addition of Er, which is ascribed to the fact that the fixed-valent Er3+ reduces the oxidation capacities of UO2+x. The enthalpy of oxygen incorporation in (Er0.06li0.94)O2+x has been evaluated from the mean valences of U-ion as -180±70 kJ/mole.

• Bulletin of the Korean Chemical Society
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• 제34권8호
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• pp.2297-2304
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• 2013

A series of novel benzimidazole derivatives bearing a heterocyclic ring as oxadiazole (21-32), thiadiazole (33-34), triazole (35-36) were synthesized and evaluated for their activities against Coxsackie virus B3 and B6 in Vero cells. Compounds 21-26, 31-36 with moieties of 2'-pyridyl, 3'-pyridyl and 4'-pyridyl at the 2-position and oxadiazoles, thiadiazole, or triazole substituent at the 4- or 5-position generally displayed activities against CVB3 and CVB6. Especially compound 24 ($IC_{50}=1.08{\mu}g/mL$, SI = 61.7 against CVB3) was the promising candidate as lead compound for anti-enteroviral drug. It was observed in the incorporation of heterocyclic rings in benzimidazole at the 5-position could enhance their biological activities.

• Journal of Industrial and Engineering Chemistry
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• 제68권
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• pp.293-300
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• 2018

Reduced graphene oxide (rGO) nanosheets were patterned with poly[benzodithiophene-bis(decyltetradecyl-thien) naphthothiadiazole] (PBDT-DTNT) and poly[bis(triiso-propylsilylethynyl) benzodithiophene-bis(decyltetradecyl-thien) naphthobisthiadiazole] (PBDT-TIPS-DTNT-DT) and used in photovoltaics. Conductive patternings changed via surface modification of rGO; because polymers encountered a high hindrance while assembling onto grafted rGO. The best records were detected in indium tin oxide (ITO):poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS):PBDTDTNT/rGO:PBDT-DTNT:LiF:Al devices, i.e., short current density $(J_{sc})=11.18mA/cm^2$, open circuit voltage $(V_{oc})=0.67V$, fill factor (FF) = 62% and power conversion efficiency (PCE) = 4.64%. PCE increased 2.31 folds after incorporation of PBDT-DTNT into thin films. Larger polymer assemblies on bared-rGO nanosheets resulted in greater phase separations.

• Geomechanics and Engineering
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• 제17권5호
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• pp.439-444
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• 2019

It is generally known that high strength soil is indicative of well-graded particle size distribution. However, there are some special cases of firm ground despite poor grade distribution, especially a specific gap-graded soil. Based on these discoveries, this study investigated the development of an additive of gap-graded soils designed to increase soil strength. This theoretical concept was used to calculate the mixed ratio required for optimal soil strength of the ground sample. The gap-graded aggregate was added according to Plato's polyhedral theory and subsequently calculated ratio and soil strength characteristics were then compared to characteristics of the original soil sample through various test results. In addition, the underground stress transfer rate was measured according to the test conditions. The test results showed that the ground settlement and stress limit thickness were reduced with the incorporation of gap-graded soil. Further field tests would confirm the reproducibility and reliability of the technology by using gap-graded soil to reinforce soft ground of a new construction site. Gap-graded soil has the potential to reduce the construction cost and time of construction compared to other reinforcing methods.