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http://dx.doi.org/10.7314/APJCP.2014.15.22.9853

Drug Resistance Effects of Ribosomal Protein L24 Overexpression in Hepatocellular Carcinoma HepG2 Cells  

Guo, Yong-Li (Oncology Department of the Jining First People's Hospital)
Kong, Qing-Sheng (Department of Scientific Research)
Liu, Hong-Sheng (Intensive Care Unit of Affiliated Hospital of Jining Medical University)
Tan, Wen-Bin (Department of Pathogenic Biology, Jining Medical University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.22, 2014 , pp. 9853-9857 More about this Journal
Abstract
Background: The morbidity and mortality rate of liver cancer continues to rise in China and advanced cases respond poorly to chemotherapy. Ribosomal protein L24 has been reported to be a potential therapeutic target whose depletion or acetylation inhibits polysome assembly and cell growth of cancer. Materials and Methods: Total RNA of cultured amycin-resistant and susceptible HepG2 cells was isolated, and real time quantitative RT-PCR were used to indicate differences between amycin-resistant and susceptible strains of HepG2 cells. Viability assays were used to determine amycin resistance in RPL24 transfected and control vector and null-transfected HepG2 cell lines. Results: The ribosomal protein L24 transcription level was 7.7 times higher in the drug-resistant HepG2 cells as compared to susceptible cells on quantitative RT-PCR analysis. This was associated with enhanced drug resistance as determined by methyl tritiated thymidine (3H-TdR) incorporation. Conclusions: The ribosomal protein L24 gene may have effects on drug resistance mechanisms in hepatocellular carcinoma HepG2 cells.
Keywords
Ribosomal protein L24; drug resistance mechanism; liver cancer; HepG2 cells;
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