• BMB Reports
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• v.30 no.6
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• pp.431-437
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• 1997

Since the role of CD40 on the interleukin-4(IL-4) -induced B cell activation has been strongly implicated in the agumentation of IgE production and response, we have investigated the intracelluar signaling pathways utilized by IL-4 and CD40 for type II IgE receptor (CD23) expression. IL-4 and anti-CD40 antibody treatment of human B cells, independently caused a rapid induction of CD23 gene activation within 2 h. There was a noticeable synergism between the action of the two agents inducing CD23 expression: the addition of anti-CD40 to the IL-4-treated culture significantly agumented the IL-4-induced CD23 on both mRNA and surface protein levels, and the inclusion of IL-4 in the anti-CD40-treated cells caused a further increase of CD23 expression far above the maximal level induced by anti-CD40. Protein tyrosine kinase (PTK) inhibitors effectively suppressed the both IL-4- and anti -CD40-induced CD23 expression. whereas protein kinase C (PKC) inhibitors had no effects. Electrophoretic mobility shift assays (EMSA) have shown that IL-4 and anti-CD40 induce the activation of NF-IL-4 and $NF-_{K}B$, respectively, binding to the CD23 promoter, both in a PKC-independent and PTK-dependent manner. These data suggest that the synergistic activation of CD23 gene expression by IL-4 and anti-CD40 is mediated by co-operative action of distinct nuclear factors. each of which is rapidly activated via PKC-independent and PTK-dependent process.

• Journal of Applied Biological Chemistry
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• v.59 no.1
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• pp.37-43
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• 2016

Bioactive components of ultra-fine ground Saururus, the extraction yield increases when the leaves are ultra-fine ground. Comparison of normal-ground and ultra-fine ground Saururus chinensis leaves showed that the solid content and antiinflammatory activity of ultra-fine ground extracts was higher than that of normal-ground extracts. Lipopolysaccharide (LPS)-stimulated Raw 264.7 cells were treated with different concentrations of Saururus chinensis extract and the amount of nitric oxide (NO) was determined; LPS-treated cells produced 2 times more NO than cells that were not treated with LPS. Moreover, the NO production in cells treated with Saururus chinensis extract was inhibited in a concentration-dependent manner. Because the stimulant-induced NO production is regulated by the inducible nitric oxide synthase (iNOS), we measured the iNOS protein level to elucidate the mechanism by which the NO production was inhibited. We found that the amount of iNOS decreased dose-dependently. It was reduced by 53% at a Saururus chinensis extract concentration of $100{\mu}g/mL$. The protein expression of cyclooxygenase-2 (COX-2) in LPS-treated Raw 264.7 cells was inhibited by 31% at $100{\mu}g/mL$ of Saururus chinensis extract. Gel shift of the nuclear factor kappa B-DNA complex occurred in LPS-treated cells and the intensity of the band decreased gradually in a concentration-dependent manner. Ultra-fine ground Saururus chinensis extract had a concentration-dependent inhibitory effect on the production of prostaglandin $E_2$, tumor necrosis factor ${\alpha}$, interleukin $1{\beta}$ (IL-$1{\beta}$), IL-6, and IL-8 in LPS-treated Raw 264.7 cells, i.e., at $50{\mu}g/mL$ of Saururus chinensis extract, their levels were decreased by 53, 67, 52, 37, and 21% respectively.

• The Journal of the Korea Contents Association
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• v.18 no.9
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• pp.217-230
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• 2018

Proficiency-centered manufacturing environment of the past is now expanding into that focused on expertise and knowledge. So the paradigm shift about human resources and management of them is required to maintain competitiveness and run the organization in a stable way. Many studies have shown that in various fields job competence has a positive influence on job satisfaction, but researchers' point of view is limited only to proficiency. With a focus on this situation, this study has analyzed empirically the effect of job knowledge and proficiency on a turnover intention and its mediating effect of job satisfaction in S company whose manufacturing environment is changing fast. The analysis has shown that the job knowledge and proficiency have a positive effect on job satisfaction and that job satisfaction influences a turnover intention. It has an important meaning in that by estimating the relationship between employees' job knowledge and their turnover intention, this study is providing a basic data needed to manage job competence of manufacturing labor force.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.11
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• pp.6782-6792
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• 2014

This study examined the effects of the symptoms of musculoskeletal disorder and the burden of the body on work stress in a university hospital workers. The research surveyed 679 subjects. The relevant factors affecting the job stress were found to be female, young people age group, unmarried, shift work, work more than 8 hours, short experience workers. The work stress was significantly higher in the non-smoker (p=0.000), regular exercise/sports(p=0.022) and non-drinking(p=0.038) groups. In addition, the mental fatigue, presence of musculoskeletal symptoms, fatigue level, and marital status increase the risk of the work stress. The results showed that the burden of the body and musculoskeletal disorders are related interactively with each other.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.2
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• pp.181-189
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• 2008

The aim of this study was to evaluate the effects of $Ca^{2+}$, $HCO_3{^-}$ and BSA on the in vitro capacitation-associated protein tyrosine phosphorylation, hyperactivation and acrosome reaction in guinea pig sperm. Caudal epididymal sperm were incubated in four different groups: modified TALP (Tyrode's albumin lactate pyruvate) or TALP without one of the medium constituents ($Ca^{2+}$, $HCO_3{^-}$ and BSA). After incubation for the required time (0 h, 0.5 h, 1 h, 3 h, 5 h, and 7 h), sperm were removed for further experiment. The capacitation effect was assessed by CTC (Chlortetracycline) staining. Western blotting and indirect immunofluorescence were used to analyze the level and localization of tyrosine phosphorylation. The results showed that guinea pig sperm underwent a time-dependent increase in protein tyrosine phosphorylation during the in vitro capacitation and the percentage of protein tyrosine phosphorylated sperm increased from 36% to 92% from the beginning of incubation to 7 h incubation. Also, there was a shift in the site of phosphotyrosine-specific fluorescence from the head of sperm to both the head and the flagellum. Moreover, an absence of $Ca^{2+}$ or $HCO_3{^-}$ inhibited in vitro hyperactivation and acrosome reaction and decreased the phosphorylation of the proteins throughout the period of in vitro capacitation. However, an absence of BSA could not influence these processes if substituted by polyvinyl alcohol (PVA) in the medium.

• Ocean and Polar Research
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• v.37 no.3
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• pp.211-223
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• 2015

Korean society has been recently promoting the restoration of coastal wetlands. These efforts might become the basis of a policy framework that compensates for the limitations of a regulation-oriented policy such as the designation of Marine Protected Areas (MPAs). The shift in government policy could contribute to strengthening the socioeconomic infrastructure of coastal development through the accumulation of ecological capital. Although our scientific efforts and social demands in regard to the ecological restoration of the coastal wetlands have increased during the past years, the bases for restoration in Korea requires that scientific, technological, financial, social and legal aspects be enhanced. The present study re-examined the concept and attitudes behind coastal wetland restoration in the light of changing circumstances in Korea. Herein, we first defined coastal wetland restoration as "An act of recovering the functions of the ecosystem of coastal wetlands to a state that resembles conditions prior to being damaged." Next, this study discussed the limitations and future directions of such restoration efforts based on the descriptive analyses of recent restoration practices from social, economic, and technological aspects. Finally, we suggest future policy directions regarding coastal wetland restoration on the basis of a PFST (Policy, Financial, Social, and Technological) analysis; 1) re-arranging legal mechanisms, 2) setting multi-dimensional restoration goals, 3) establishing a multi-discipline- and convergence based R&D system, 4) linking spatial management and local development to the restoration, 5) building restoration governance at the local level, 6) implementing an ecosystem service payment system, and 7) applying test-bed projects in accordance with proper directions.

• Proceedings of the Korea Contents Association Conference
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• 2007.11a
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• pp.183-187
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• 2007

During the recent decades in democratic and industrialized countries, government policies for governing technological innovation and risks to environment and human health have undergone significant changes. The shift from a top-down legislative approach to a more inclusive and deliberative atmosphere is, putatively the most prominent change. Such a move is often described as a move from government to governance. In the governance of technology and risk, public engagement has been a major strategy in technology decision-making process. This article aims to look into the changes in the procedural modes of technology decision-making process. It discusses the main viewpoints that have been placed on the basis of such a move. Also, it further relates the changes in local decision-making process to science and technology decision-making at global level. It argues that the democratic and reflexive trends in local science and technology decision-making will be the basis upon which to shape and respond to global governance system: while international decision-making process would require accountability in integrating different values and rationalities, such accountability may be sustained and reinforced depending on the robustness of the local decisions and social choices.

• Journal of the Korean Magnetic Resonance Society
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• v.17 no.1
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• pp.40-46
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• 2013

For a case study of suspected paraquat intoxication, we developed a simple and rapid method of $^1H$ qNMR to determine the mili-molar amount of paraquat in postmortem blood samples. There were no interfering signals from endogenous compounds in the chemical shift of paraquat and diquat (internal standard). The amount of sample used ranged from 0.25 mM to 10.0 mM. Diquat, which has similar physicochemical properties with paraquat, was chosen as an internal standard. The NMR experimental conditions, relaxation delay time and CPMG spin-echo pulse sequence were optimized. The developed method was validated in terms of specificity, accuracy, precision, matrix effect, recovery, limit of detection (LOD), and low limit of quantification (LLOQ). The proposed qNMR method provided a simple and rapid assay for the identification and quantification of the quaternary ammonium herbicide, "paraquat" in postmortem blood samples. This method was tested by using the blood from the heart of a man who was intoxicated with paraquat. In this particular case, the level of paraquat was 1.07 mM in the blood. For the determination of quaternary ammonium herbicides, qNMR could also be used to provide a better understanding of the currently available techniques.

• Journal of Korea Technology Innovation Society
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• v.9 no.3
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• pp.558-577
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• 2006

With the rapid shift to a knowledge-based economy, science and technology (S&T) is considered as the principle drivers in a nation's competitiveness. All countries are focused on upgrading the level of their S&T of nations and strengthening their national competitiveness. With these factors in mind, Korea has increased its support of national research and development (R&D). As a result of this added support, Korea S&T has made great strides. This improvement can be seen in the drastic increase in the number of Korean researchers who have been published in Nature, Science and Cell (NSC), three top journals in the fields of S&T. This study is a quantitative analysis on the NSC Publications of Korean researchers. The purpose of this study is to derive research policy suggestions to improve Korea's national competitiveness in S&T.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.31 no.5A
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• pp.484-492
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• 2006

A multi-code biorthogonal code keying(MBCK) system consists of multiple waveform coding block, and the sum of output codewords is transmitted. Drawback of MBCK is that it requires amplifier with high linearity because its output symbol is multi-level. MBCK with constant amplitude preceding blcok(CA-MBCK) has been proposed, which guarantees sum of orthogonal codes to have constant amplitude. Redundant bits of CA-MBCK for constant amplitude coding are not only used to make constant amplitude signal but also used to improve the bit error rate(BER) performance at receiver. In this paper, we proposed a transmission scheme which combine CA-MBCK with $Q^{2}PSK$ to improve bandwidth efficiency of CA-MBCK. The BER performance of the scheme is same that of CA-MBCK in additive white gaussian noise(AWGN). And we showed that BER performance of the proposed system can be improved using redundant bits of constant amplitude preceding.