• Journal of Life Science
• /
• v.9 no.2
• /
• pp.153-159
• /
• 1999

To express constitutively the inulinase gene (INUl) of Kluyveromyces marxianus in Saccharomyces cerevisiae, three yeast promoters such as GAPDH, ADH1 and ENO1 were connected upstream of INUl. The resulting plasmids, pYIGP, pADHl-INU, and pENO-INU were introduced to S. cerevisiae SEY2102 host strain, respectively, and then each transformants were selected by staining of colonies on sucrose-agar plate. When the yeast transformants were cultivated on 2$\%$ dextrose media, the total expression levels of inulinase reached to 1.11 unit/mL, 0.88 unit/mL, and 0.69 unit/mL for respective GAPDH, ADH1, and ENO1 promoter systems. On 4% dextrose media, however, the inulinase activities were observed at 2.00 unit/mL for pYIGP, 0.71 unit/mL for pADH1-INU, and 1.40 unit/mL for pENO-INU. This result indicates that the constitutive expression of INUl was significantly affected by the initial concentration of dextrose and the promoter strength was in the order GAPDH, ENO1, and ADH1 promoter at high dextrose concentration. Taking into account the plasmid stability, however, it is suggested that the ENO1 promoter system is more suitable for the INU1 expression on high dextrose medium or in the fed-batch cultivation accumulating ethanol at high level.

• Journal of Korean Society of Steel Construction
• /
• v.17 no.6 s.79
• /
• pp.673-679
• /
• 2005

According to continuous welded rails on a bridge, temperature changes bring about the expansion of the bridge deck,adding axial forces on the track. Moreover, the ballast on the bridge deck expansion joint is moved due to the bridge deck. The longer the bridge deck is, the greater the influence will be, loosening the ballast, causing track irregularities, and deteriorating passenger comfort. Considering the structure of the bridge itself and tolerance for track irregularities caused by the loosened ballast on the bridge, the maximum length of the deck should be less than 80 m, which is the same as the standard of French railways. In this study, the interaction between the expansion related to the bridge length and the irregularity in the longitudinal level, referring to measurements and maintenance work performed in high-speed railways, was analyzed. This research shows that the installation of a sliding plate or a vertical ballast stopper is not a good option, since it is difficult. On the other hand, the installation of a ZLR fastener or gluing is easy, but its influence is insignificant. In conclusion, switch tie tamping or manual tamping is more effective than other methods of what?

• Journal of the Korean Institute of Gas
• /
• v.9 no.4 s.29
• /
• pp.57-61
• /
• 2005

The objective of paper is to carry out a comparative Quantitative Risk Assessment (QRA) of two KOGAS tank designs using a fault tree methodology, a standard 'Full Containment' tank and a 'Membrane' tank. For the membrane tank, both the initial KOGAS design and 4 modified KOGAS designs have been assessed, giving six separate cases. In this paper, the frequencies of releases are quantified using a fault tree approach. For clarity in the analysis, and to ensure consistency, all cases have been quantified using the same fault tree. Logic within the fault tree is used to select each of the cases. Full quantification of risks is often difficult, owing to a lack of relevant failure data, but the aim of this study has been to be as quantitative as possible, with full transparency of failure information. The most significant general cause of external LNG leaks is predicted to be a seismic event, which has been quantified nominally. 4modified KOGAS desiens to Prevent damage of bottom membrane panels that was shown in preparatory estimation could quantitively confirm safety improvement. According to result, the predicted frequencies of an external LNG leak for the full containment and modified membrane tanks are very similar, failures due to dropped pumps are predicted to be significantly greater for the membrane tank with thickened plate than for the full containment tank.

• Journal of Nutrition and Health
• /
• v.17 no.4
• /
• pp.245-252
• /
• 1984

The aim of the present experiment was to investigate the effects of anionization on the various stage of mice growth, the feeding level of diets, the switch - over effects, effects of plane of nutrition on growth of mice, the effects of method of ionization on growth of mice and associated breeding performance. Mice in polyethylene cages were placed on vinyl plate charged anion by an Electrical Ionizing Machine. At an early growing stages body weight gains of mice anionized were not different from the unionized group. The dietary intake did not affect significantly the body weight of mice 15- l7g. The growth of mice of 11.5-16.2g was not affected by ionization statistically, but the ionized group increased slightly. The switching over from the untreated to the ionization slightly depressed the ionized mice by 2.4g, the untreated switched over to ionization group by 2.28g as compared with the 3.29g for the untreated during the 4- day - feeding period. Plane of nutrition and ionization did not significantly affect the growth of mice of 7.1-15.8g when mice were fed high - protein and high - carbohydrate diets. The Common Ionization Method was appeared statistically superior to the Overnight - Ionization Method. The growth rate of the offspring of mice ionized prior to conception was found to be normal but the number of progeny was considerablly high.

• The Journal of the Korea Contents Association
• /
• v.11 no.6
• /
• pp.270-278
• /
• 2011

The purpose of this study was to compare acute and chronic LBP patient in twenties and forties, respectively by size measure paraspinal muscle (cross-sectional area; CSA). CSA of paraspinal muscle (psoas, multifidus, erector muscle) size was measured by free-handling technique of the picture archiving and communication system(PACS) using MRI at the level(lower end-plate of L4) in twenties(9 males, 10 females) and forties(9 males, 8 females) in acute and chronic LBP patient. The results of this study showed no significantly difference between acute and chronic LBP (p>0.05) in twenties patients. However, there was significant difference between acute and chronic LBP (p<0.05) in forties patients. Also, there was significant difference in paraspinal muscle CSA between chronic LBP patients in twenties and chronic LBP patients in forties (p<0.05). This study showed that paraspinal muscle atrophy was observed in forties with various cause, but Not chronic LBP patients in twenties. Accordingly it is required for chronic LBP patients in forties to minimize trunk muscle atrophy through immediate back muscle dynamic exercise and early functional activity.

• Korean Journal of Soil Science and Fertilizer
• /
• v.23 no.3
• /
• pp.173-179
• /
• 1990

The effect of a highly water absorbing polyacrylate polymer, commonly called K-sorb, at rates of 0.0, 0.05, 0.2, and 0.5% by weight on the water retention properties of three soils, and the longevities of these treatment effects were evaluated. Water retentions were measured for all the treatments by use of a pressure-plate extractor in the laboratory. Available water and three-phase distributions at moisture tensions of 0.01, 0.3, and 15b were calculated from water retentivity data. A randomized block experiment of Chinese cabbages was conducted to examine the effects and the longevities of the treatments(0.0, 0.05, 0.1, and 0.2%) on water retention of Jungdong sandy loam soil in the field. Water retentions for a loamy sand, sandy loam, and loam soil, treated with 0.2 and 0.5% K-sorb, were increased. K-sorb treatments were more effective in sandy soil than in loamy soils. Water contents for the 0.5% treatment were markedly greater than those for the 0.2% treatment at earth moisture tension. K-sorb only at a rate of 0.5% remained effective in water retention of each soil through repeated drying and wetting for 12 months. Duncan's multiple range showed 0.2% treatment was effective(at the level of 0.05) after 2 months but not after 10 months under field condition.

• Journal of the Korean Vacuum Society
• /
• v.16 no.6
• /
• pp.424-432
• /
• 2007

Luminance properties of external electrode fluorescent lamps and optical components in backlight unit (BLU) and optical transmission rates of optical sheets, are investigated for LCD-TV of 32" in diagonal with WXGA level resolution (1366$\times$768). The luminance is measured in 12-lamps and 18-lamps of BLU. The luminance uniformity preserves about 85 % in the 12-lamps backlight, while the luminance of optical components and the efficiency of backlight in the 12-lamps are lower than those in the 18-lamps backlight. When the lamp luminance in BLU having 12-lamps and 18-lamps is normalized as 100 %, the relative luminance of a diffusion plate, a diffusion sheet, a prism sheet (BEF), a polarization sheet (DBEF), has a constant value without dependence on a lamp luminance. The relative luminance of optical components in 12-lamps BLU is lower than that in 18-lamps backlight. The light transmission rate, the relative luminance of liquid crystal display panel with the luminance 100 % of backlight, is 7.14 % in the use of DBEF and BEF, 6.12 % in the use of only DBEF, and 3.21 % in the use of only BEF. Those Data obtained in this experiment for the lamps and optical components, are the design parameters for the LCD backlight.

• Microbiology and Biotechnology Letters
• /
• v.28 no.2
• /
• pp.117-123
• /
• 2000

The object of this work is to improve the maintenance of bioluminescence from stored Photobacterium phosphoreum in a view of developing continuous monitoring system for pollutants. The long-term experiments were performed to determine the effect of storage temperature and immobilization on the maintenance of bioluminescence and viability of P. phosphoreum. A naturally luminescent bacterium, P. phosphoreum was starved in 2.5% Nael solution at $20^{\circ}C$, $4^{\circ}C$, -$20^{\circ}C$ and -$70^{\circ}C$ for 30 days. In vivo luminescence was measured by luminometry, and total cell concentrations and concentrations of culturable and viable cells were determined by acridine orange staining, dilution plate counting, and direct viable counting, respectively. The bioluminescence emission from cells stored at 4De was maintained up to 10 days while those with starved cells at other temperature ranges decreased to background level within 3 days. In terms of viability of cells, concentrations of cells stored at $20^{\circ}C$ were rapidly decreased as a result of cell lysis, leading to a drop in culturable and viable counts while cells stored at $4^{\circ}C$ was shown viable but nonculturable state during starvation. With immobilized cells on strontium alginate, the bioluminescence showed higher maintenance than free cells and decreased with count number of nonculturable cells.

• Microbiology and Biotechnology Letters
• /
• v.35 no.1
• /
• pp.11-16
• /
• 2007

The arylsulfatase gene (astA, 984 bp ORF) from Pseudoalteromonas carrageenovora genome was amplified by PCR and subcloned into the pHCE-IA vector, in which the hyper consitutive expression (HCE) promoter from the D-amino acid aminotransferase (D-AAT) gene of Geobacillus toevii was employed. The transformant cell, Escherichia coli BL21 (DE3)/pHCE-AST, on LB agar plate containig 4-methylumbelliferyl sulfate, showed an intense fluorescence at 360 nm, indicating that 4-methylumbelliferone was liberated by desulfatate activity. When BL21 (DE3)/pHCE-AST was grown on LB media containing 0.4% glucose or 0.4% glycerol, the arylsulfatase activity was higher at glycerol rather than at glucose. On 2% glycerol medium, the arylsulfatase activity reached 15.0 unit/ml, which was 2.6-fold higher expression level than that with 1% glycerol. The DNA ladder in agarose prepared from agar by this recombinant enzyme revealed similar resolution and migration patterns with a commercial agarose. This results suggests that arylsulfatase overexpressed in E. coli could be applicable to the economic production of electrophoretic-grade agarose.

• Journal of Microbiology and Biotechnology
• /
• v.21 no.12
• /
• pp.1280-1286
• /
• 2011

A quantitative, real-time PCR method was developed to enumerate Lactobacillus plantarum IWBT B 188 during the malolactic fermentation (MLF) in Grauburgunder wine. The qRT-PCR was strain-specific, as it was based on primers targeting a plasmid DNA sequence, or it was L. plantarum-specific, as it targeted a chromosomally located plantaricin gene sequence. Two 50 l wine fermentations were prepared. One was inoculated with 15 g/hl Saccharomyces cerevisiae, followed by L. plantarum IWBT B 188 at $3.6{\times}10^6$ CFU/ml, whereas the other was not inoculated (control). Viable cell counts were performed for up to 25 days on MRS agar, and the same cells were enumerated by qRT-PCR with both the plasmid or chromosomally encoded gene primers. The L. plantarum strain survived under the harsh conditions in the wine fermentation at levels above $10^5$/ml for approx. 10 days, after which cell numbers decreased to levels of $10^3$ CFU/ml at day 25, and to below the detection limit after day 25. In the control, no lactic acid bacteria could be detected throughout the fermentation, with the exception of two sampling points where ca. $1{\times}10^2$ CFU/ml was detected. The minimum detection level for quantitative PCR in this study was $1{\times}10^2$ to $1{\times}10^3$ CFU/ml. The qRT-PCR results determined generally overestimated the plate count results by about 1 log unit, probably as a result of the presence of DNA from dead cells. Overall, qRT-PCR appeared to be well suited for specifically enumerating Lactobacillus plantarum starter cultures in the MLF in wine.