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Constitutive Expression of Arylsulfatase from Pseudoalteromonas carageenovora in E. coli and Its Application to Preparation of Agarose  

Kim, Mi-Jin (Department of Biotechnology and Bioengineering, Department of Biomaterial Control, Dong-Eui University)
Jang, Yhon-Hwa (Department of Biotechnology and Bioengineering, Department of Biomaterial Control, Dong-Eui University)
Sung, Moon-Hee (BioLeaders Corp)
Kim, Yeon-Hee (Department of Biotechnology, Osaka University)
Nam, Soo-Wan (Department of Biotechnology and Bioengineering, Department of Biomaterial Control, Dong-Eui University)
Publication Information
Microbiology and Biotechnology Letters / v.35, no.1, 2007 , pp. 11-16 More about this Journal
Abstract
The arylsulfatase gene (astA, 984 bp ORF) from Pseudoalteromonas carrageenovora genome was amplified by PCR and subcloned into the pHCE-IA vector, in which the hyper consitutive expression (HCE) promoter from the D-amino acid aminotransferase (D-AAT) gene of Geobacillus toevii was employed. The transformant cell, Escherichia coli BL21 (DE3)/pHCE-AST, on LB agar plate containig 4-methylumbelliferyl sulfate, showed an intense fluorescence at 360 nm, indicating that 4-methylumbelliferone was liberated by desulfatate activity. When BL21 (DE3)/pHCE-AST was grown on LB media containing 0.4% glucose or 0.4% glycerol, the arylsulfatase activity was higher at glycerol rather than at glucose. On 2% glycerol medium, the arylsulfatase activity reached 15.0 unit/ml, which was 2.6-fold higher expression level than that with 1% glycerol. The DNA ladder in agarose prepared from agar by this recombinant enzyme revealed similar resolution and migration patterns with a commercial agarose. This results suggests that arylsulfatase overexpressed in E. coli could be applicable to the economic production of electrophoretic-grade agarose.
Keywords
Arylsulfatase; constitutive HCE promoter; Pseudoalteromonas carrageenovora; agarose; E. coli;
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1 Araki, C. 1937. Agar-agar. III. Acetylation of the agar-like substance of Gelidium amansii(L). J. Chem. Soc. Japan. 58: 1338-1350
2 Barbeyron, T., P. Potin, C. Richard, O. Collin, and B. Kloareg. 1995. Arylsulphatase from Alteromonas carrageenovora. Microbiology 141: 2897-2904   DOI   ScienceOn
3 Beil, S., H. Kehrli, J. Peter, W. Staudenmann, A. M. Cook, T. Leisinger, and M. A. Kertesz. 1995. Purification and characterization of the agaropectin sulfatase synthesized by Psedomonas aeruginosa PAO during growth in sulfate-free medium and cloning of the arylsulfatase gene (atsA). Eur. J. Biochem. 229: 385-394   DOI   ScienceOn
4 De Hostos, E. L., R. K. Togasaki, and A. Grossman. 1988. Purification and biosynthesis of a derepressible periplasmic arylsulfatase from Chlamydomonas reinharditii. J. Cell Biol. 106: 29-37   DOI   ScienceOn
5 Do, J. R. and S. W. Oh. 1999. Preparation of agarose from Gelidium amansii for gel electrophoresis using various purification methods and its resolution characteristics for DNA. Kor. J. Food Sci. Technol. 31: 110-114
6 Dodgson, K. S. and R. G Price. 1963. A note on the deternimation of the ester sulfate content of sulfated polysaccarides. Biochem. J. 84: 350-356
7 Henderson, M. J. and F. H. Milazzo. 1979. Arylsulfatase in salmonella typhymurium: Detection and influence of carbon source and tyramine on its synthesis. J. Bacteriol. 139: 80-87   PUBMED
8 Kim, J. H., D. S. Byun, J. S. Godber, J. S. Choi, W. C. Choi, and H. R. Kim. 2004. Purification and characterization of arylsulfatase from Sphingomonas sp. AS6330. Appl. Microbiol. Biotechnol. 63: 553-559   DOI
9 Mackie, W. and R. D. Preston. 1974. Cell wall and intracellular region polysaccarides. In: Stewart WDP (eds) Algal physiology and biochemistry. Antarct. Sci. 13: 64-65
10 Do, J. R., J. H. Park, and K. S. Jo. 1998. A manufacturing technique of agar with strong gelling ability from Gelidium amansii. J. Kor. Fish. Soc. 31: 673-676   과학기술학회마을
11 Guiseley, K. B. 1970. The relationship between methoxyl content and gelling temperature of agarose. Carbohydr. Res. 13: 247-256   DOI   ScienceOn
12 Jeong, K. J., J. H. Choi, W. M. Yoo, K. C. Keum, N. C. Yoo, S. Y. Lee, and M. H. Sung. 2004. Constitutive production of human leptin by fed-batch culture of recombinant rpoS-Escherichia coli. Protein Expr. Purif. 36: 150-156   DOI   ScienceOn
13 Jansen, H. J., C. A. Hart, J. M. Rhodes, J. R. Saunders, and J. W. Smalley. 1999. A novel mucin-sulphatase activity found in Bukholderia cepacia and Pseudomonas aeruginosa. J. Med. Microbiol. 48: 551-557   DOI   ScienceOn
14 Melo, M. R. S., J. P. A. Feitosa, A. L. P. Freitas, and R. C. M. de Paula. 2002. Isolation and characterization of soluble sulfated polysaccaride from the red seaweed Gracilaria cornea. Carbohydr. Polym. 49: 491-498   DOI   ScienceOn
15 Renn, D. 1997. Biotechnology and the red seaweed polysaccharide industry: status, needs and prospects. Trends Biotechnol. 15: 9-14   DOI   ScienceOn
16 Duckworth, M. and W. Yaphe. 1971. The structure agar. Part 1. The fractionation of a complex mixture of polysaccarides. Carbohydr. Res. 16: 189-197   DOI   ScienceOn
17 Fielding, A. H. and G Russell. 1974. The Competitive properties of marine algae in culture. J. Ecol. 62: 689-698   DOI   ScienceOn
18 Lim, J. M., Y. H. Jang, H. R. Kim, Y. T. Kim, T. J. Choi,J. K. Kim, and S. W. Nam. 2004. Overexpression of arylsulfatase in E. coli and its application to desulfatation of agar. J. Microbiol. Biotechnol. 14: 777-782
19 Yoon, H. S. and Y. H. Park. 1984. Studies on the composition of agarose and agartin in agar-agar. Bull Kor. Fish. Soc. 24: 27-33
20 Guiseley, K. B., F. H. Kirpatrick, R. B. Provonchee, M. M. Dumais, and S. Nochumson. 1993. A further fractionation of agarose. Hydrobiologia 260: 505-511   DOI
21 Haryonug, P., J. J. Song, S. P. Hong, Y. H. Choi, S. W. Yun, J. H. Kim, S. C. Lee, S. G Lee, and M. H. Sung. 2002. Novel high-level constitutive expression system, pHCE vector, for a convenient and cost-effective soluble production of humal tumor necrosis factor-a. Biotechnol. Lett. 24: 1185-1189   DOI   ScienceOn
22 Delisle, G. and F. H. Milazzo. 1970. The isolation of arylsulphatase isoenzymes from pseudomonas aeruginosa. Biochim. Biophys. Acta 212: 505-508   DOI   PUBMED   ScienceOn
23 Hoshi, M. and T. Moriya. 1980. Arylsulfatase of sea-urchin sperm. 2. Arylsulfatase as a lysin of sea-urchins. Dev. Biol. 74: 343-350   DOI   ScienceOn
24 Miech, C., T. Dierks, T. Selmer, K. V. Figura, and B. Schmidt. 1998. Arylsulfatase from Klebsiella pneumoniae-carries a formylglycine generated from a serine. J. Biol. Chem. 273: 4835-4837   DOI   ScienceOn
25 Milanesi, A. A. and J. W. C. Bind. 1972. Lysosomal enzymes in aquatic species II. Distribution and particle properties of thermally acclimated muscle Iysosomes of rainbow trout Salmo gairdeneri. Comp. Biochem. Physiol. 41: 473-491
26 Flemenger, G., B. Solomon, T. Wolf, and E. Hadas. 1990. Effect of polyethylene glycol on the non-specific adsorption of proteins to Eupergit C and agarose. J. Chromatogr. 510: 271-279   DOI   ScienceOn
27 Izumi, K. 1970. A new method for fractionation of agar. Agr. Biochem. 34: 1739-1740
28 Do, J. R. 1997. Extraction and purification of agar from Gelidium amansii. J. Kor. Fish. Soc. 30: 423-427
29 Qingping, L., Y. L. Shen, D. Z. Wei, and W. Cao. 2006. Optimization of culture on the overproduction of TRAIL in high-ceIl-density culture by recombinant Escherichia coli. Appl. Microbiol. Biotechnol. 71: 184-191   DOI
30 Allan, G., P. G. Johnson, Y. Lay, and K. V. Sarkanen. 1971. Marine polymers; Part 1. A new procedure for the fractionation of agar. Carbohydr. Res. 17: 234-236   DOI   ScienceOn
31 Akagawa-Matsushita, M., M. Matsuo, Y. Koga, and K. Yamasato. 1992. Alteromonas atlantica sp. nov. and Alteromonas carrageenovora sp. nov., bacteria that decompose algal polysaccharides. Int. J. Syst. Bacteriol. 42: 621-627   DOI
32 Lee, H. S., C. Rhee, and H. C. Yang. 1985. A study on th purification by protein precipitants and washing of agar. Kor. J. Food Sci. Technol. 17: 340-344   과학기술학회마을