• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.3
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• pp.1-12
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• 2006

Purpose : This study was aimed to investigate the inhibitory effect of Leonurus sibiricus on the proliferation of human uterine leiomyoma cells and the expression of gene related the mechanism of cell apoptosis. Methods : We counted the number of death cells treated with indicated concentration of Leonurus sibiricus and investigated cell death rate by MTS assay. Furthermore, flow cytometry analysis and DNA fragmentation assay were used to dissect between necrosis and apoptosis and then we observed the differential gene expression by western blot analysis. Results : Leonurus sibiricus significantly inhibited the proliferation of uterine leiomyoma cell in a dose-dependent and time dependent manner. Fluorescence activated cell sorter (FACS) analysis indicated that Leonurus sibiricus induced G1 cell cycle arrest. Leonurus sibiricus enhanced the expression of p27 and p53 with cell cycle arrest. Conclusion : These findings suggest that Leonurus sibiricus is a candidate agent for the treatment of uterine leiomyoma. p27, $p53^{1}$ may play an important role in Leonurus sibiricus-induced cell cycle arrest and cell growth inhibition.

• The Korea Journal of Herbology
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• v.32 no.5
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• pp.13-18
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• 2017

Objectives : The aim of this study is to investigate anti-inflammation of Leonurus sibiricus methanol extract against UVB-damage in fibroblast. The skin is continuously exposed to damage from environmental stresses. UV radiation causes a variety of biological effects especially on the skin, including inflammation and photoaging. Methods : In this study, we tried to search for Leonurus sibiricus which exhibit protective activities against UVB-induced cytotoxicity and oxidative cell death, NO and $PGE_2$ production. HS68 cells were exposed to UVB ($120mJ/cm^2$) and treated with various concentrations (0, 0.25, 0.5, 1, 2, 4, $8mg/m{\ell}$) of Leonurus sibiricus methanol extract for additional 24 h. Intracellular reactive oxygen species (ROS) levels generated by UV radiation were detected using a spectrofluorometer after DCF-DA staining. Also, HS68 cells were irradiated with UVB and then treated with Leonurus sibiricus methanol extract for 12 h. The lipid peroxidation was assayed by measuring the levels of 8-isoprostane secreted into the culture medium. Results : UVB-induced cytotoxicity and cell death were effectively suppressed by treatment of Leonurus sibiricus aqueous methanol extracts. Oxidative cell damage was mediated $PGE_2$ in UVB-induced HS68 fibroblast cell, which was significantly inhibited by treatment with Leonurus sibiricus extracts. Also, the protective effect of these extract seemed to be mediated by inhibited intracellular ROS generation and lipid peroxidation in dose-dependent manner. Conclusion : These results suggest that Leonurus sibiricus aqueous methanol extracts may have anti-aging effects new functional materials against oxidative UVB stress-mediated skin damages.

• Journal of the Korean Society of Food Science and Nutrition
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• v.15 no.4
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• pp.47-50
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• 1986

These studies were carried out to investigate effects of Leonurus sibiricus green juice on gastric secretion of rats and acute toxicity in mice ; $LD_50(mg/kg)$ of Leonurus sibiricus extract was 625mg/kg intraperitoneally in mice. The groups administered green juice and water extract of Leonurus sibiricus showed more excellent increasing effect in total acidity and peptic activity, and decreasing effect on gastric volume than control group. But any differences were not found between sample and control group in gastric pH. It is suggested that Leonurus sibiricus can he used as the bitter stomachics to promote the gastric digestion.

• Korean Journal of Pharmacognosy
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• v.15 no.2
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• pp.104-107
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• 1984

The essential oil fraction of Leonurus sibiricus was analyzed by TLC and gas chromatography. By utilizing silica gel column, a ketone compound, m.w. 167, was isolated from the essential oil. The essential oil showed considerably the diuretic action, but the water extract exhibited weak action. This diuretic action of the water extract was potentiated by combined administration of essential oil. On the isolated rabbit's uterus the essential oil decreased spontanous movement and showed relaxation.

• 대한한약학회:학술대회논문집
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• 2007.11a
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• pp.145-160
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• 2007

• The Plant Pathology Journal
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• v.32 no.1
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• pp.65-69
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• 2016

In plant virus ecology, weeds are regarded as wild reservoirs of viruses and as potential sources for insect-mediated transmission of viruses. During field surveys in 2013-2014, three Leonurus sibiricus plants showing virus-like symptoms were collected from pepper fields in Daegu, Seosan, and Danyang in Korea. Molecular diagnosis assays showed that the collected L. sibiricus samples were infected with either Tomato spotted wilt virus (TSWV), Pepper mild mottle virus (PMMoV), or Beet western yellow virus (BWYV), respectively. Since this is the first identification of TSWV, PMMoV, and BWYV from L. sibiricus, complete genome sequences of three virus isolates were determined to examine their phylogenetic relationships with the previously reported strains and isolates. Phylogenetic analyses performed using full genome sequences of the viruses showed the isolates of TSWV and PMMoV obtained from L. sibiricus are closely related to the pepper isolates of the corresponding viruses. Our results suggest that L. sibiricus could act an alternative host and reservoir of viruses that cause damages in pepper fields.

• Korean Journal of Animal Reproduction
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• v.19 no.4
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• pp.245-250
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• 1996

This study was undertaken to illustrate the uterotonic effect of Leonurus sibiricus. It was dissolved in sterile water and several different dosages were administered both in vitro and in vivo study. Rat uterine tissue for in vitro bioassay was obtained from estrous rat. From the low to high dosages of Leonurus sibiricus were tried and each uterine contraction was recorded and integrated. Anesthetized estrous rat for in vivo study was cannulated into the jugular vein for infusion of the compound. Another cannula with a balloon tipped and water filled was inserted into the uterus to measure uterine activity. While the uterine tissue did not respond to low dosage of compound, high dosage of compound stimulated the tissue to contract less than 1 minute with low amplitude. In vivo rat uterus showed a certain, consistent pattern of contractions which was initial relaxation and followed by prolonged and increased amplitude of contractions. It also caused a short breathing stop which might be due to acute acidosis.

• Food Science and Preservation
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• v.20 no.4
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• pp.592-596
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• 2013

To stabilize the basal extraction conditions of Leonuri herbal products, three kinds of Leonurus sibiricus water extracts were prepared with different extraction conditions (boiling extraction (I), high thermal process & boiling extraction (II), and wet grinding & boiling extraction (III)), and their physiological properties were investigated. The extraction yields of the L. sibiricus water extracts were from 13.02 to 15.90%, with no significant difference among them. The polyphenol contents were significantly high in extracts (II) and (III) than in extract (I). The $IC_{50}$ for the electron-donating ability was the smallest in extracts (II), (III) and (I) in ascending order. The ABTS radical scavenging ability was significantly higher in extracts (II) and (III) than in extract (I). Also, the ACE in hibition ability for 5 mg/mL of each extract was high in extracts (II) and (III), but there was no significant difference among the three extracts in terms of their nitrite scavenging abilities. Extraction processes (II) and (III) were shown to be useful for preparing Leonurus sibiricus water extracts with healthful properties.

• Research in Plant Disease
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• v.8 no.2
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• pp.113-116
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• 2002

A sclerotinia rot of Leonurus sibiricus caused by Sclerotinia sclerotio겨m occurred severely in farmer's fields at Seosang-myon, Hamyang-gun, Gyeongnam Province, Korea in 2001. The typical symptoms of the disease were that leaves, stems or collar of the infected plants at first became darker green and then watery soft rotted, and white fluffy mycelia formed on the lesion developed into black sclerotia later, Sclerotia on the plant and PDA medium were globose to cylindrical or irregular in shape, and 0.8~10.3$\times$0.8~6.4 m in size. Aphothecia with numerous asci were cup-shape, and 0.6~1.2 cm in diameter, Asci with 8 spores were cylindrical, and 87~246$\times$4.6~21.6 $\mu\textrm{m}$ in size. Ascospores of one cell were hyaline, ellipsoid to ovoid in shape, and 10.2~14.6$\times$4.7~7.2 $\mu\textrm{m}$ in size. The range of temperature for mycelial growth was from 5 to 3$0^{\circ}C$, and the optimum was $25^{\circ}C$. This is the first report on the sclerotinia rot of L. sibiricus caused by Sclerotinia sclerotioum in Korea.

• Korean Journal of Pharmacognosy
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• v.38 no.2 s.149
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• pp.148-151
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• 2007

Ig Mo Cho (益母草) has been used as remedy for invigorating blood circulation to remove blood stasis and relieving edema by inducing diuresis. With regard to the botanical origin of Ig Mo Cho, it has been considered to be Leonurus spiecies of Labiatae, but there has never been pharmacognostical confirmation on it. To clarify the botanical origin of Ig Mo Cho from Korea, the anatomical characteristics of Leonurus japonicus and L. sibiricus were studied. As a result, the botanical origin of Ig Mo Cho from Korea was proved the herba of Leonurus japonicus.