• The Korean Journal of Food And Nutrition
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• v.20 no.4
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• pp.341-348
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• 2007

The antioxidative effect and antimutagenic capacity in ethanol extracts of Lentinus edodes were studied for suggestion of prevention and dietetic treatment of chronic diseases and development of antioxidative and antimutagenic functional food by employing biological and biochemical assay. The $IC_{50}$ of MDA with BSA conjugation reaction, lipid peroxidation and scavenging effect on DPPH radical in ethanol extracts of Lentinus edodes showed 74.58 mg/assay, 5.747 mg/assay and 0.939 mg/assay respectively. So, the most effective antioxidative capacity in ethanol extracts of Lentinus edodes was the scavenging effect on DPPH radical, among the method used this study. The indirect and direct antimutagenic effects of ethanol extracts of Lentinus edodes were examined by Ames test using Salmonella typimurium TA98 and TA100. The inhibition rates on indirect mutagenicity mediated by 2-anthramine showed 91.67% in the Salmonella typimurium TA98 and 96.60% in the Salmonella typimurium TA100. The inhibitory effect on direct mutagenicity mediated by sodium azide in Salmonella typimurium TA100 was 22.83%. and mediated by 2-nitrofluorene in Salmonella typimurium TA98 was 5.34%. This data indicates that ethanol extracts of Lentinus edodes have more effective effects on indirect mutagenicity than direct mutagenicity. From this result, it believed to have a possible antioxidative and antimutagenic capacities, and taken for the candidate of prevention and dietetic treatment of chronic diseases and development of antioxidative and antimutagenic functional food.

• Korean Journal of Food Science and Technology
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• v.20 no.4
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• pp.459-462
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• 1988

Compositions of free-sugars and sugar-alcohols in three cultivated mushrooms, Pleurotus ostreatus, Lentinus edodes and Agaricus bisporus were analyzed by gas-liquid chromatography and compositional differences of these components depending on species, sizes of fruiting body and portions(pileus and stipe) were investigated. Glucose, fructose, trehalose, glycerol, arabitol and mannitol were identified in each of the three mushrooms. The major components were mannitol and arabitol in Lentinus edodes, mannitol in Agaricus bisporus, trehalose and mannitol in Pleurotus ostreatus. The total free-sugars and sugar-alcohols content were in the range of 12.7-18.9% in Lentinus edodes, 9.2-16.1% in Agaricus bisporus and 8.2-10.6% in Pleurotus ostreatus. Total contents of these components in Pleurotus ostreatus and Agaricus bisporus were higher in pileus than those in stipe and it was decreased as pileus size development in all of three edible mushrooms.

• The Korean Journal of Community Living Science
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• v.16 no.1
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• pp.105-111
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• 2005

This study was carried out to investigate the effect of Lentinus edodes which were harvested at different times of maturity on blood glucose and lipid levels in diabetic mice. The diabetic KK mice were fed diets supplemented with Lentinus edodes harvested early (LE) or late (LL) for eight weeks, and control (CO) mice were fed regular diet without Lentinus edodes. Diet intake, body weight, organ weight, blood glucose and serum lipid levels were measured. The mice which were fed diets supplemented with LE and LL showed significantly lower body weights compared to the CO group. There were no significant differences in the diet intake and the weights of different organs including liver, heart, kidneys, and epididymal fat pad among three different groups. Furthermore, the concentrations of serum triglycerides and total cholesterol were significantly lower in LE and LL groups compared to the CO group. These results clearly demonstrate that Lentinus edodes, regardless of their harvested period reduced body weight and serum lipid without affecting blood glucose level in diabetic mice.

• The Korean Journal of Mycology
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• v.15 no.1
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• pp.42-47
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• 1987

4 strains of the oak mushroom Lentinus edodes including the strain ASI 3046 were collected home and abroad to investigate the characterics of mycelial growth on synthetic media and sawdust media. The possibility of artificial culture of Lentinus edodes on media composed of different saw dusts and adding materials was investigated. Results were as follows: The optimum temperature for growth ranged from $22^{\circ}C$ to $26^{\circ}C$ among the lineages of Lentinus edodes. The optimal growth temperature for ASI 3046 strain and ASI 3047 strain was $26^{\circ}C$ and $22^{\circ}C$, respectively. The optimal carbon source for ASI 3046 strain was glucose, and peptone and ammoinum tartrate were proved excellent as nitrogen sources. When Lentinus edodes was cultured on sawdust media by varying the mixing ratio of different sawdusts and adding materials, mixed sawdust media composed of oak sawdust(50%, v/v), poplar sawdust(50%, v/v), acorn powder(5%, v/v) and rice bran(10%, v/v) showed higher yields of fruit bodies. Results showed that the ideal strain of Lentinus edodes for artificial culture on sawdust media was strain ASI 3046.

• Korean Journal of Food Science and Technology
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• v.30 no.3
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• pp.702-708
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• 1998

The effects of mushrooms such as Lentinus edodes and Pleurotus ostreatus on anti-cancer activity through in vivo and in vitro experiments were powders of protein-bound polysaccharides in mushrooms were solubilized in 0, 5, 25 mg/kg saline, respectively and were used in vitro experiments. The in vivo experiments were carried out as followed: i) anti-cancer activities on Leukemia $(L_{1210})$, Hepatlicus cancer $(H_{22})$ and Sarcoma180/(S180), and ii) the effect on immune system through changes in intestine weight and the number of hemolytic plague forming cells. Protein-bound polysaccharides of all showed anti-cancer activity on $L_{1210}$ and fruit body of Lentinus edodes 25 mg/kg treatment group showed the highest inhibition rate (86%). Pleurotus ostreatus mycelial in medium of cultivate 25 mg/kg treatment. Fruid body of Lentinus edodes 25 mg/kg treatment group showed the highest inhibition rate (86% and 71%, respectively) on $H_{22}$ among them. The inhibition rates of fruit body and mycelial of Lentinus edodes 25 mg/kg treatment groups on S180 were 33.9% and 30.9%, respectively. Each samples of 50, 100, 200, $400\;{\mu}g/{\mu}L$ on in vitro cell toxicity test did not show significantly different cell death rates at P<0.05. In immune test, weights of liver and spleen were increased according to increase in conc. but were not significantly different at P<0.05. The weights of thymus were heavy in fruit body and mycelial of Lentinus edodes treatment group but were not significantly different at P<0.05. Hemolytic plague forming cells with antibody formation capability were significantly high in fruit body and mycelial of Lentinus edodes treatment samples.

• The Korean Journal of Mycology
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• v.20 no.1
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• pp.77-82
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• 1992

Researches were carried out to find the optimal conditions of carbon sources, nitrogen sources and pH for the maximum production of sporophore of Lentinus edodes. Dextrin, aspartic acid and pH 4.0 were the best conditions for yield of sporophore by using replacement culture technique. The production of sporophore was stimulated by addition of 0.8% triacylglycerol in NS medium. Coffee waste was chosen for the best substrate among the poplar, oak, white aspen saw dust and coffee waste. Increased growth of mycelim and yield of sporophore was obsewed by adding tannin up to 0.1% as substrate.

• Journal of the Korean Wood Science and Technology
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• v.21 no.1
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• pp.39-50
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• 1993

The purpose of this study was to describe the micromorphological changes in Korean red pine (Pinus densiflora S. et Z.) wood decayed by a major brown-rot fungus, Lentinus lepideus, using scanning electron microscope and transmission electron microscope. At the end of the 12-week exposure to the fungus in soil block procedure(ASTM 1971), test blocks sustained 5.02% weight loss. The formation of bore hole by hyphae and penetration of hyphae through bordered pit were not observed. Instead, fungal hyphae appeared to penetrate axially tracheid luminar from the the ray cells via cross field pits. Hyphae were mainly found in lignin rich cell corner regions of tracheids, and also extensive degradation of tracheid wall occurred in this region. Extensive degradation of $S_2$ layer occurred without noticeable alteration of the $S_3$ layer, but warty layer and compound middle lamella remained relatively intact. Localized erosion, the characteristic of white rot, was observed in some cell wall and wall components including lignin were found to be decomposed.

• The Korean Journal of Mycology
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• v.19 no.3
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• pp.214-219
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• 1991

Adenosine-5'-triphosphatase$(F_1-ATPase)$ in the Lentinus edodes was fractionated by ammonium aulfate 30% saturation and purified by Sephadex G-200 gel filtration in three times. Three kinds of protein fractions of $F_1-ATPase$ were isolated from this mushroom, and fraction I and ll showed its activity for the substrate, adenosine-5'-triphosphate. Its optimum pH and temperature were found to be pH 7.6 and $58^{\circ}C$, and its thermal stability was stabled for 30 min. at $20-30^{\circ}C$. The Km value of this enzyme was 1.81 mM.

• Journal of Nutrition and Health
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• v.30 no.7
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• pp.743-750
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• 1997

The hypoglycemic effects of 2 mushrooms, Pleurotus ostreatus and Lentinus edodes, on streptozotocin(STZ) induced diabetic rats were investigated in this study . Diabets mellitus was induced in male Sprague-Dawley rats by the injection of STZinto the tail vein at a dose of 45mg/kg. Sprague-Dawley male rats(200-250g) were assigned to one control and three STZ-diabetic groups. Diabetic groups were assigned to STZ-control, pleurotus ostreatus and Lenitinus edodes groups. All groups were fed a AIN 76 diet. The two experimental groups were fed with each protein-bound polysaccharide(150mg/kg BW) for 14 days and with carboxymethyl cellulose for STZ-control group. The body weight gain was monitored and the blood levels of glucose and cholesterol were measured . Levels of protein triglyceride, and free fatty acid in plasma were analysed. Serum aminotransferase activity as also measured. The body weight gain was lower in the all diabetic groups than in the of normal group. The weight of spleen was reduced by adminstration of the Lentinus edodes protein-bound polysaccharides. The result suggest that orally administered Lentinus edodes protein-bound polysaccharides exhibited hypoglycemic effect in STZ -induced diabetic rats and that these protein-bound polysaccharides may be useful for the management of diabetes mellitus.

• Journal of Environmental Health Sciences
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• v.17 no.1
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• pp.129-135
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• 1991

To investigate on the trace element content of twelve edible mushrooms and Aloe arborescens, i. e., Lentinus edodes, Ganoderma lucidum (culturing in wood and soil), Tricholoma matsutake, Agaricus bisporus, Cyrophora esculenta, Auricularia auricula-Jude (produced in Korea and China), Sarcodon asparatus, Pleurotus ostreatus, Coriolus versicolor, Smilax rotundifolia and Aloe arborescerts were analyzed by Atomic absorption spectrometer. The obtained results were summerized as follows: 1. Potassium, sodium, magnesium and iron content for the most part samples were in large quantities, especially phosphorus content of those was highest ammount for the all samples. 2. Sodium content was much ammount in the Lentinus edodes (39mg) and Ganoderma lucidurn (20 mg), Culturing in wood and soil, while potassium was very high ammount in the Aloe arborescens and other samples. Mush ammount of magnesium as compared with others was Lentinus edodes (144mg), Ganoderma lucidurn (128mg), Aloe arborescerts (50mg) and pleurotus ostreatus (60mg). Phosphorus content of Ganoderma lucidurn, Lentinus edodes, Gyrophora esculenta, Auricularia polytricha and Agaricus bisporus was much ammount while iron content of all samples equality higher ammount. Sodium content of Aloe arborescens was not analyzed out for almost all, its potassium (82mg), magnesium (50mg) and iron (18rng) content comparatively higher quentity than others minerals and phosphorus volume (4.9mg) as compared with others, was conspicuously lower detect. 4. Cadimium and lead content of harmful metal element were detected on trace quentity for the most part samples 5. Organic acids of samples i.e., Legtinus edodes, Agaricus bisporus, Pleurotus ostreatus and Ganoderma lucidum were Citrate, Malate, Fumalate, Succinate, Oxalate, Acetate, Lactate, and Tartarate and Citrate, Malate and Fumarate contents were higher amount remarkbly than other organic acids. Tartarate content was trace amount.