The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Antioxidative Stress and Antimutagenic Effects of Lentinus edodes Ethanol Extracts

표고버섯 에탄올 추출물의 산화적 스트레스 억제 효과와 항돌연변이 효과

  • Oh, Se-In (Dept. of Food and Nutrition, Seoil College) ;
  • Lee, Mee-Sook (Dept. of Food and Nutrition, Hannam University)
  • Published : 2007.12.31
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Abstract

The antioxidative effect and antimutagenic capacity in ethanol extracts of Lentinus edodes were studied for suggestion of prevention and dietetic treatment of chronic diseases and development of antioxidative and antimutagenic functional food by employing biological and biochemical assay. The $IC_{50}$ of MDA with BSA conjugation reaction, lipid peroxidation and scavenging effect on DPPH radical in ethanol extracts of Lentinus edodes showed 74.58 mg/assay, 5.747 mg/assay and 0.939 mg/assay respectively. So, the most effective antioxidative capacity in ethanol extracts of Lentinus edodes was the scavenging effect on DPPH radical, among the method used this study. The indirect and direct antimutagenic effects of ethanol extracts of Lentinus edodes were examined by Ames test using Salmonella typimurium TA98 and TA100. The inhibition rates on indirect mutagenicity mediated by 2-anthramine showed 91.67% in the Salmonella typimurium TA98 and 96.60% in the Salmonella typimurium TA100. The inhibitory effect on direct mutagenicity mediated by sodium azide in Salmonella typimurium TA100 was 22.83%. and mediated by 2-nitrofluorene in Salmonella typimurium TA98 was 5.34%. This data indicates that ethanol extracts of Lentinus edodes have more effective effects on indirect mutagenicity than direct mutagenicity. From this result, it believed to have a possible antioxidative and antimutagenic capacities, and taken for the candidate of prevention and dietetic treatment of chronic diseases and development of antioxidative and antimutagenic functional food.

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References

  1. Lee, IS, Nishikawa, A. Polyzellus multiplex, a Korean wide mushroom, as a potent chemopreventive agent against stomach cancer. Life Sci. 73:3225-3234. 2003 https://doi.org/10.1016/j.lfs.2003.06.006
  2. Cheung, LM, Cheung, PCK and Ooi, VEC. Antioxidant activity and total phenolics of edible mushroom extracts. Food Chern. 80:1-7. 2003 https://doi.org/10.1016/S0308-8146(02)00227-3
  3. Ham, SS, Oh, SW, Kim, YK, Shin, KS, Chang, KY, Chung, GH. Antimutagenic and cytotoxic effects of ethanol extract from the Inonotus obliquus. J Kor. Soc. Food Sci. Nutr. 32:1088-1094. 2003 https://doi.org/10.3746/jkfn.2003.32.7.1088
  4. Konno, S, Tortorelis, DG, Fullerton, SA, Samadi, AA, Hettiarachchi, J, Tazaki, H. A possible hypoglycemic effect of Maitake mushroom on type 2 diabetic patients. Diabetic Medicine. 18: 1010-1011. 2001 https://doi.org/10.1046/j.1464-5491.2001.00532-5.x
  5. Kim, BK, Shin, GG, Jeong, BS, Cha, JY. Cholesterol-lowering effect of mushrooms power in hyperlipidemic rats. J. Kor. Soc. Food Sci. Nutr. 30:510-515. 2001
  6. Hong, JS and Kim, TH. Contents of free amino acids and total amino acids in Agaricus bisporus, Pleurotus ostreatus and Lentinus edodes. Kor. J. Food Sci. Technol. 21:58-62. 1989
  7. Song, JY, Yoon, KJ, Yoon, HK and Koo, S1. Effects of $\beta$-glucan from Lentinus edodes and Hordeum vulgare on blood glucose and lipid composition in alloxan-induced diabetic mice. Kor. J. Food Sci. Technol. 33:802-807. 2001
  8. Kweon, MH, Kwon, ST, Kwon, SH, Ma, MS and Park, YI. Lowering effects in plasma cholesterol and body weight by mycelial extracts of two mushroom: Agaricus blazai and Lentinus edodes. Kor. J. Microbiol. Biotechnol. 30: 402-409. 2002
  9. Choi, MY, Lim, SS and Chung, TY. The effects of hot water soluble polysaccharides from Lentinus edodes on lipid metabolism in the rats fed butter yellow. J. Kor. Soc. Food Sci. Nutr. 29:294-299. 2000
  10. Hwang, KH, Kim, HK and Han, TN. Inhibitory activity of edible mushrooms on the tissue thromboplastin (tissue factor). Kor. J. Food Sci. Technol. 29:161-166. 1997
  11. Lee, SH, Park, HJ, Cho, SY and Jeong, HJ. Supplementary effect of Lentinus edodes on serum and hepatic levels in spontaneously hypertensive rat. Kor. Nutr. Soc. 37: 507-514. 2004
  12. Yang, BK, Kim, DH and Song, CH. Production of Lentinus edodes mycelia in submerged culture and it's hypoglycemic effect in diabetic rats. Kor. J. Mycology. 30:131-135. 2002 https://doi.org/10.4489/KJM.2002.30.2.131
  13. Cho, YJ, Kim, HA, Bang, MA and Kim, ER Effects of dietary mushroom on blood glucose levels, lipid concentration and glutathione enzymes in Streptozotocin-induced diabetic rats. Kor. Nutr. Soc. 35:183-191. 2002
  14. Park, MK, Jin, YG, Kim, DG, Jin, JY and Lee, YJ Effects of Lentinus edodes extract on the loperarnide-induced constipation in rats. Kor. J. Food Sci. Technol. 39:88-93. 2007
  15. Han, MJ, Bae, EA, Rhee, YK and Kim, DH Effect of mushrooms on the growth of intestinal lactic acid bacteria. Kor. J. Food Sci. Technol. 28:947-952. 1996
  16. Han, MJ, Bae, EA, Rhee, YK and Kim, DH Effect of Lentinus edodes on some enzymes of intestinal bacteria. Kor. J Food Sci. Technol. 33: 142-145. 2001
  17. Lee, BW and Park, KM. Antitumor activity of protein-bound polysaccharides extracted from mycelia of Lentinus edodes. Kor. J Food Sci. Technol. 30:665-671. 1998
  18. Park, JM, Lee, SH, Kim, JO, Park, HJ, Park, JB and Sin, Jl, In vitro and in vivo effects of extracts of Lentinus edodes on tumor growth in human papillomavirus 16 oncogene-transformed animal tumor model. Kor. J Food Sci. Technol. 36:141-146. 2004
  19. Hwang, YJ, Nam, HK, Chang, MJ, Noh, GW and Kim, SH Effect of Lentinus edodes. and pleurotus eryngii extracts on proliferation and apoptosis in human colon cancer cell lines. J Kor. Soc. Food Sci. Nutr. 32:217-222. 2003 https://doi.org/10.3746/jkfn.2003.32.2.217
  20. Park, MH, Oh, KY and Lee, BW. Anticancer activity of Lentinus edodes. and Pleurotus astreatus Kor. J Food Sci. Technol. 30:702-708. 1998
  21. Chihara, G. Immune modulation agents and their mechanism(lentinan, a T celloriented immunopotentiator). Basal. 19:409-439. 1985
  22. Comporti, M. Lipid peroxidation: An overview. In: Free Radicals: From Basic Science to Medicine: Molecular and cell biology updates(Poli G, Albano E & Dianzani MU, eds.), pp.65-79. Birkhauser Verlag, Basel, Switzerland 1993
  23. Meneghini, R, Martins, EAL and Calderaro, M DNA damage by reactive oxygen species: The role of metal. In: Free Radicals: From Basic Science to Medicine: Molecular and cell biology updates(poli G, Albano E & Dianzani MU, eds.), pp.102-111. Birkhauser Verlag, Basel, Switzerland. 1993
  24. Lee, GD, Chang, HG and Kim, HK. Antioxidative and nitrite-scavenging activities of edible mushroom Kor. J. Food Sci. Technol. 29:432-436. 1997
  25. Park, YH. Effect of polyamine on modification of biomodics by aldehyde. PhD in Medicine Thesis. Seoul National University. 2000
  26. Gomez-Sanchez, A, Hermonsin, I and Mayo, I. Cleavage andoligomerization of malondialdehyde under physiological conditions. Tetrahedron Letters. 28:4077-4080. 1990
  27. Saija, A, Scalese, M, Lanza, M, Marzullo, D, Bonina, F and Castelli, F. Flavonoids as antioxidant agents: Importance of their interaction with biomembranes. Free Radical Biology & Medicine. 19:481-486. 1995 https://doi.org/10.1016/0891-5849(94)00240-K
  28. Haase, G and Dunkley, WL. Ascorbic acid and copper in 1ino1eate oxidation. L Measurement of oxidation by ultraviolet spectrophotometry and the thiobarbituric acid test. J. Lipid Research. 10:555-560. 1969
  29. Chen, HM, Muramoto, K, Yamauchi, F, Fujimoto, K and Nokihara, K Antioxidative properties of histidine-containing peptides designed from peptide fragments found in the di-gests of a soybean protein. J. Agricultural & Food Chem. 46:49-53. 1998 https://doi.org/10.1021/jf970649w
  30. Maron, DM and Ames, BN. Revised methods for the Salmonella rmnagenicity test. Mutat. Res. 113:173-215. 1983 https://doi.org/10.1016/0165-1161(83)90010-9
  31. Mutsushima, T, Sugimura, T, Nagao, M, Yahagi, T, Shirai, A and Sawamura, M. Factors modulating mutagenicity in microbial test, In 'Short-term test, systems for detecting carcinogens'. Norphth KH and Gamer RC (eds.), Springer, Berling, p. 273. 1980
  32. Kang, MY, Kim, SY, Yun, HJ and Narn, SH. Antioxidative activity of the extracts from browned oak mushroom (Lentinus edodes) with unmarketable quality. Korean J. Food Sci. Technol 36:648-654. 2004
  33. Lee, GD, Chang, HG and Kim, HK. Antioxidative and nitrite-scavenging activities of edible mushroom Kor. J. Food Technol.. 29:432-436. 1997
  34. Oh, SI and Lee, MS. Antioxidative and antimutagenic effects of Ganoderma lucidum krast extracts. Kor. J. Food & Nutr. 18:54-62. 2005
  35. Kang, YH, Park, YK and Lee, GD. The nitrite scavenging and electron donating ability of phenolic compounds. Kor. J. Food Sci. Technol. 28:232-239. 1996
  36. Kang, MY, Kim, SY, Yun, HJ and Narn, SH. Screening of physiological functionality for unmarketable browned oak mushroom(Lentinus edodes). J. Kor. Soc. Appl. Biol. Chem. 47:396-402. 2004