• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.6
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• pp.477-481
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• 2004

In bean sprout culture water imbibition and benzyladenopurine (BA) treatment are done at the same time. The study was carried out to check the effect of treatment period (3, 5, and 7 hours) on growth of mungbean (cv. Keumseongnogdu, Owoolnogdu, and Zhong Lu 1) sprouts and to analyse its absorption amount on the base of their moisture content. The 3 cultivar seeds were soaked in 50 ppm BA solution immediately before 3 hour aeration and then cultured for 6 days. The sprouts were sorted by 4 categories on the base of hypocotyl length; > 7cm, 4 to 7cm < 4cm, and non-germination, and their morphological characters, fresh and dry weights were measured. The cultivar Zhong Lu 1 had the highest rate in longer than 7cm hypocotyls of the three cultivars but the lowest one in shorter than 4cm. Rates of the above 4 categories in cv. Keumseongnogdu, Owoolnogdu showed no significant difference between the treatment periods while one of longer than 7cm hypocotyls in cv. Zhong Lu1 was decreased with longer treatment period. Lateral roots were less formed with longer treatment period, especially as lengthened from 3 to 5 hours. Hypocotyl and root were also lengthened with longer treatment period and hypocotyl was more thickened in 5 hour treatment period than in the two others. Total fresh and dry weights per sprout showed no significant difference between treatment periods although cv. Zhong Lu1 relatively faster grew than the other cultivars. In the case of shorter than 5hour treatment periods the absorption amount of BA was the greatest in cv. Zhong Lu1 but in 7 hour treatment period it was the greatest in cv. Keumseongnogdu and Zhong Lu 1.

• Journal of Chest Surgery
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• v.37 no.9
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• pp.749-754
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• 2004

Off-pump coronary artery bypass grafting (Off-Pump CABG) has been proven to have less morbidity and to facilitate early recovery. High-risk surgical patients may have benefitted by avoiding the adverse effects of the cardiopulmonary bypass. We compared the effectiveness of Off-Pump CABG with that of coronary artery bypass using cardiopulmonary bypass (On-Pump CABG) in high-risk patients. Material and Method: 682 patients (424 Off-Pump CABG and 258 On-Pump CABG) underwent isolated coronary artery bypass grafting between January 2001 and June 2003. Patients who were considered high risk were selected High risk is defined as the presence of one or more of nine adverse prognostic factors. Data were collected from 492 patients in Off-Pump CABG and 100 in On-Pump CABG for risk factors, extent of coronary disease, and in-hospital outcomes. Result： Off-Pump CABG group and On-Pump CABG group did not show differences in their preoperative risk factors. We used more arterial grafts in Off-Pump CABG group (p < 0.05). Postoperative results showed that operative mortality (0.5% in Off-Pump CABG versus 2.0% in On-Pump CABG), renal failure (2.6% in Off-Pump CABG versus 7.0% in On-Pump CABG), and perioperative myocardial infarction (1.5% in Off-Pump CABG versus 1.0% in On-Pump CABG) did not differ significantly. However, Off-Pump CABG had shorter mean operation time (p<0.05), lower mean CK-MB level (p <0.05), lower rate of usage of inotropics (p < 0.05), shorter mean ventilation time (p <0.05), lower perioperative stroke (0% versus 2.0%), and shorter length of stay (p < 0.05) than On-Pump CABG. On-Pump CABG had more distal grafts (p<0.05) than Off-Pump CABG. Although Off-Pump CABG and On-Pump CABG did not show statistical differences in mortality and morbidity was more frequent in CABG. Conclusion: Off-Pump CABG reduces morbidity and favors hospital outcomes. Therefore, Off-Pump CABG is safe, reasonable and may be a preferable operative strategy for high-risk patients.

• Journal of Chest Surgery
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• v.42 no.3
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• pp.344-349
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• 2009

Background: The clinical history and physical findings of the patients with spontaneous pneumothorax depend largely on the extent of the collapse of the lung and the presence of pre-existing pulmonary disease. Large primary spontaneous pneumothorax is a possible serious condition and. so more active treatment will be necessary for these patients. The therapeutic guideline for large pneumothorax remains controversial. Therefore, by assessing the clinical results of surgical treatment for large primary pneumothorax, we aim to determine the indicators of treatment. Material and Method: Among 348 patients with primary spontaneous pneumothorax and who underwent surgical treatment from August 2004 through December 2007, 58 patients who responded to treatment for a large primary pneumothorax were included in the current study. We then retrospectively evaluated the operative findings and the surgical results. The patients with a pneumothorax of 80% or more, including those patients with tension pneumothorax, were considered to have a "large pneumothorax". Most of these patients Should be treated with a 12F chest tube. Thoracoscopic wedge resection was considered for treating recurrent pneumothorax, continuous air leakage, controlateral pneumothorax and first episode pneumothorax with visible blebs (> 1cm) seen on the computed tomography. Result: There were 50 men and 8 women with a mean age of 28.2 years (range: $14\sim54$ years). The mean length of hospitalization was 5.3 days (range: $2\sim10$ days). Nine patients underwent chest tube drainage only. Forty-nine patients underwent thoracoscopic wedge resection. The mean follow up time was 27.8 months (range: $10\sim58$ months). The actual site of air leakage could be located in 35 patients (71.4%) and this was correlated with pleural adhesion (p=0.005). The initial air leakage tended to be more correlated with intra-operative air leakage, although this was not statistically significant (p=0.066). The recurrence rate was 11.1 % for the patients with chest tube drainage and 2.0% for the patients with thoracoscopic wedge resection. Conclusion: Large primary pneumothorax requires an early diagnosis and early treatment. Thoracoscopic wedge resection may help to prevent recurrence of large primary pneumothorax.

• Korean Journal of Medicinal Crop Science
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• v.5 no.3
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• pp.225-231
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• 1997

This study was conducted to evaluate the growth and yield by sowing times and spacing using machine seeder on Coix Lachryma-jobi L. var ma-yuen $S_{TAPF}$. Adlay was seeded at four different dates (April 20, May 5, May 20 and June 5). Planting spaces were controlled by the seed roller of tractor drill seeder attached to tractor at three spacing ($60{\times}15cm,\;70{\times}15cm\;and\;80{\times}15cm$). The results were summarized as follows. Required days to emergence and days to anthesis were shortened as sowing date was late. But days to maturity were prolonged when sowing date was late. The accumulated temperature increased such as required periods increased. In growth characteristics, culm length was significantly different at different sowing times. But all growth characteristics was not affected by different spacings. In yield components, 1,000 grain weight and ripening rate were significantly different at different sowing times, also the number of tillers and branches was significantly affected at different spacings. Grain yield/ha was significantly different at different sowing times. But it was not significantly different at different spacings. Thus, in order to improve the yield of adlay using drill seeder, if it is not frost, sowing should be done as early as possible. As a result, suitable sowing time was April 20 with spacing at $60{\times}15cm$ using machine seeder in Korea.

• Korean Journal of Medicinal Crop Science
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• v.7 no.3
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• pp.147-154
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• 1999

This research was carried out to obtain the field resistance to disease and pest, and high yielding of new Boxthorn (Lycium chinense Mill.) varieties. Growth characteristics, yield potential, correlation of trait which was related the yield and path coefficient of the domestic native varieties. two of cultivated varieties and four of introduced varieties were investigated. In growth characters of collected varieties, the stem length of collected varieties in China were slightly long. Leaf area in Jindo, Kumsan native and Yusong 1 and 2 were wider than that of other varieties. Resistance to pest iniury was very strong at China 1, Japan 1 and Yusong 2 in eriophyidmite, and also resistance to anthrax anthracnose were showed less than 10% of the rate of attack fruit at Japan 1 and China 1. Flowering stage was more early in Chungyang native than that of other varieties. Fruit size of Kumsan native, Yusong 2 were large size fruit varieties. Fruit setting number was most abundant at 1,623 in Yusong 2 which was collected variety. And dry yields of fruit per lOa was more cmparatively abundant in Yusong 2 at 86% than those of Chungyang native. The high correlation cofficienct character with fruit yield were fruit setting and branching number and leaf area. Direct effect by path anaslysis were fruit setting number (Py=0.45), and branching number was showed indirect effect.

• Journal of Applied Biological Chemistry
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• v.53 no.2
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• pp.65-70
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• 2010

Medium-chain fatty acids (MCFA) are composed of 8-12 carbon atoms, and are found in coconut, cuphea, and palm kernel oil. MCFA were introduced into clinical nutrition in the 1950s for dietary treatment of malabsorption syndromes because of their rapid absorption and solubility. Recently, MCFA have been applied to Gastrointestinal Permeation Enhancement Technology (GIPET), which is one of the most important parts in drug delivery system in therapeutics. Therefore, to accumulate the MCFA in seed oil of rapeseed, much effort has been conducted by classical or molecular breeding. Laurate can be successfully accumulated up to 60 mol% in the seed oil of rapeseed by the expression of bay thioesterase (Uc FatB1) alone or crossed with a line over-expressing the coconut lysophosphatidic acid acyltransferase (LPAAT) under the control of a napin seed-storage protein promoter. Also, caprylate and caprate were obtained 7 mol% and 29 mol%, respectively, from plants over-expressing of the medium-chain specific thioesterase (Ch FatB2) alone or together with the chain-length-specific condensing enzyme (Ch KASIV). Despite the success of some research in utilizing parallel classical and molecular breeding to produce MCFA, commercially available seed oils have for the most part, not been realized. Recent research in the field of developing MCFA-enriched transgenic plants has established that there is no single rate-limiting step in the production of the target fatty acids. The purpose of this article is to review some of the recent progress in understanding the mechanism and regulation of MCFA production in seed oil of rapeseed.

• Korean Journal of Weed Science
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• v.11 no.3
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• pp.174-177
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• 1991

This study was conducted to determine the influence of dithiopyr on growth of transplanted rice with different transplanting depths and the amount of $^{14}C$-dithiopyr adsorpted in the root and shoot of rice plants under paddy soil conditions. The growth rate of transplanted rice was lower in 0 and 0.25cm of transplanting depths with exposed basal stem than in 1 and 2cm of the depths in control plot. In the growth of transplanted rice treated with dithiopyr, plant hight and dry weight were significantly inhibited in 0 and 0.25cm depth plots but not affected.in 0.5, 1, 2 and 4cm depth plots, and roop length were influenced in 0, 0.25 and 4cm depth plots but not in 0.5, 1 and 2cm depth plots. The amount of ratioactivity in shoot and root of rice plants as affected by $^{14}C$-dithiopyr were the highest in 0 and 0.25cm depth plots and decreased in over 0.5cm depth plots. However the extent in amount of distributed radioactivity in the plants among the different transplanting depths was narrow gradually with the growth of plants. Therefore, injury of transplanted rice by dithiopyr is little in over 0.5cm transplanting depth with burried basal stem and the inhibition on rice plants with extreme shallow transplanting such as 0 and 0.25cm depths should be due to more adsorption of dithiopyr.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.30 no.6
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• pp.465-473
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• 2004

Purpose : The essential triad for nerve regeneration is nerve conduit, supporting cell and neurotrophic factor. In order to improve the peripheral nerve regeneration, we used polyglycolic acid(PGA) tube and brain-derived neurotrophic factor(BDNF) gene transfected Schwann cells in sciatic nerve defects of SD rat. Materials and methods : Nerve conduits were made with PGA sheet and outer surface was coated with poly(lactic-co-glycolic acid) for mechanical strength and control the resorption rate. The diameter of conduit was 1.8mm and the length was 17mm Schwann cells were harvested from dorsal root ganglion(DRG) of SD rat aged 1 day. Schwann cells were cultured on the PGA sheet to test the biocompatibility adhesion of Schwann cell. Human BDNF gene was obtained from cDNA library and amplified using PCR. BDNF gene was inserted into E1 deleted region of adenovirus shuttle vector, pAACCMVpARS. BDNF-adenovirus was multiplied in 293 cells and purified. The BDNF-Adenovirus was then infected to the cultured Schwann cells. Left sciatic nerve of SD rat (250g weighing) was exposed and 14mm defects were made. After bridging the defect with PGA conduit, culture medium(MEM), Schwann cells or BDNF-Adenovirus infected Schwann cells were injected into the lumen of conduit, respectively. 12 weeks after operation, gait analysis for sciatic function index, electrophysiology and histomorphometry was performed. Results : Cultured Schwann cells were well adhered to PGA sheet. Sciatic index of BDNF transfected group was $-53.66{\pm}13.43$ which was the best among three groups. The threshold of compound action potential was between 800 to $1000{\mu}A$ in experimental groups which is about 10 times higher than normal sciatic nerve. Conduction velocity and peak voltage of action potential of BDNF group was the highest among experimental groups. The myelin thickness and axonal density of BDNF group was significantly greater than the other groups. Conclusion : BDNF gene transfected Schwann cells could regenerate the sciatic nerve gap(14mm) of rat successfully.

• Journal of Bio-Environment Control
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• v.8 no.1
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• pp.56-66
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• 1999

Growth of Dendranthema grandiflorum R. ‘Bongwhang’plantlets, as affected by three levels of photosynthetic photon flux (PPF), 70, 150 and 220 $\mu$mol. $m^{-2}$ . $s^{-1}$ , three levels of C $O_{2}$ concentration, 400-500 (ambient), 1000 and 2000 $\mu$mol.mo $l^{-1}$ , and two levels of number of air exchanges per hour (NAEH), 0.1 $h^{-1}$ and 2.8 $h^{-l}$, was studied. Explants were obtained from photomixotrophically-micropropagated plantlets. Four explants were planted in each 3.7$\times$10$^{-4}$ $m^{3}$ polycarbonate box containing MS medium supplemented with 1.25 meq. $L^{-1}$ $H_{2}$P $O_{4}$$^{[-10]}$ and no added sugar. Explants were cultured under cool-white fluorescent lamps (16 h. $d^{-1}$ ), at 25$\pm$1$^{\circ}C$ temperature, and 70-80% relative humidity. In treatments of 2.8 $h^{-1}$ NAEH, a 10 mm round hole made on the vessel cap was sealed with a microporous filter For higher C $O_{2}$ concentrations in the culture room, C $O_{2}$ gas was provided from a tank of liquefied C $O_{2}$. Fresh and dry weights, height, length of the longest roots, number of leaves, and leaf area significantly increased with increasing PPF and especially, with increasing C $O_{2}$ concentration. Growth was enhanced with increased number of air exchanges per hour (2.8 $h^{-1}$ ). Overall, treatment of 220$\mu$mol. $m^{-2}$ . $s^{-1}$ PPF combined with 2000$\mu$mol.mo $l^{-1}$ C $O_{2}$ and 2.8 $h^{-1}$ NAEH gave the most vigorous growth of Dendranthema grandiflorum R. ‘Bongwhang’ plantlets in vitro.o.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.3
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• pp.599-610
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• 2016

This paper reports the results of a systematic review (SR) and meta-analysis research to compare the effect of Kangaroo care, targeting mothers and premature infants. A randomized clinical trial study was performed until February 2015. The domestic literature contained the non-randomized clinical trial research without restriction according to the level of the study design. A search of the Ovid-Medline, CINAHL, PubMed and KoreaMed, the National Library of KOREA, the National Assembly Library, NDSL, KISS and RISS. Through the KMbase we searched and combined the main term ((kangaroo OR KC OR skin-to-skin) AND (care OR contact)) AND (infant OR preterm OR Low Birth Weight OR LBW), ((kangaroo OR kangaroo OR kangaroo) AND (care OR nursing care OR management OR skin contact)) was made; these were all combined with a keywords search through the selection process. They were excluded in the final 25 studies (n=3051). A methodology checklist for randomized controlled trials (RCTs) designed by SIGN (Scottish Intercollegiate Guidelines Network) was utilized to assess the risk of bias. The overall risk of bias was regarded as low. In 16 studies that were evaluated as a grade of "++", 9 studies were evaluated as a grade of "+". As a result of meta-analysis, kangaroo care regarding the effects of premature mortality, severe infection/sepsis had an insignificant effect. Hyperthermia incidence, growth and development (height and weight), mother-infant attachment, hypothermia incidence, length of hospital days, breast feeding rate, sleeping, anxiety, confidence, and gratification of mothering role were considered significant. In satisfaction of the role performance, depression and stress presented contradictory research results for individual studies showing overall significant difference. This study has some limitations due to the few RCTs comparing kangaroo care in the country. Therefore, further RCTs comparing kangaroo care should be conducted.