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http://dx.doi.org/10.3839/jabc.2010.012

Production of Medium-chain Fatty Acids in Brassica napus by Biotechnology  

Roh, Kyung-Hee (Department of Agricultural Bio-resources, National Academy of Agricultural Science, RDA)
Lee, Ki-Jong (Department of Agricultural Bio-resources, National Academy of Agricultural Science, RDA)
Park, Jong-Sug (Department of Agricultural Bio-resources, National Academy of Agricultural Science, RDA)
Kim, Hyun-Uk (Department of Agricultural Bio-resources, National Academy of Agricultural Science, RDA)
Lee, Kyeong-Ryeol (Department of Agricultural Bio-resources, National Academy of Agricultural Science, RDA)
Kim, Jong-Bum (Department of Agricultural Bio-resources, National Academy of Agricultural Science, RDA)
Publication Information
Journal of Applied Biological Chemistry / v.53, no.2, 2010 , pp. 65-70 More about this Journal
Abstract
Medium-chain fatty acids (MCFA) are composed of 8-12 carbon atoms, and are found in coconut, cuphea, and palm kernel oil. MCFA were introduced into clinical nutrition in the 1950s for dietary treatment of malabsorption syndromes because of their rapid absorption and solubility. Recently, MCFA have been applied to Gastrointestinal Permeation Enhancement Technology (GIPET), which is one of the most important parts in drug delivery system in therapeutics. Therefore, to accumulate the MCFA in seed oil of rapeseed, much effort has been conducted by classical or molecular breeding. Laurate can be successfully accumulated up to 60 mol% in the seed oil of rapeseed by the expression of bay thioesterase (Uc FatB1) alone or crossed with a line over-expressing the coconut lysophosphatidic acid acyltransferase (LPAAT) under the control of a napin seed-storage protein promoter. Also, caprylate and caprate were obtained 7 mol% and 29 mol%, respectively, from plants over-expressing of the medium-chain specific thioesterase (Ch FatB2) alone or together with the chain-length-specific condensing enzyme (Ch KASIV). Despite the success of some research in utilizing parallel classical and molecular breeding to produce MCFA, commercially available seed oils have for the most part, not been realized. Recent research in the field of developing MCFA-enriched transgenic plants has established that there is no single rate-limiting step in the production of the target fatty acids. The purpose of this article is to review some of the recent progress in understanding the mechanism and regulation of MCFA production in seed oil of rapeseed.
Keywords
Brassica napus; Cuphea oil; medium-chain fatty acid; rapeseed;
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1 Dehesh K, Tai H, Edwards P, Byrne J, and Jaworski JG (2001) Overexprssion of 3-ketoacyl-acyl-carrier protein synthase IIIs in plants reduces the rate of lipid synthesis. Plant Physiol 125, 1103-1114.   DOI   ScienceOn
2 Drexler H, Spiekermann P, Meyer A, Domergue F, Zank T, Sperling P, Abbadi A, and Heinz E (2003) Metabolic engineering of fatty acids for breeding of new oilseed crops: strategies, problems and first results. J Plant Physiol 160, 779- 802   DOI   ScienceOn
3 Han J, Luhs W, Sonntag K, Zahringer U, Borchardt DS, Wolter FP,Heinz E, and Frentzen M (2001) Functional characterization of $\beta$-ketoacyl-CoA synthase genes from Brassica napus L. Plant Mol Biol 46, 229-239.   DOI   ScienceOn
4 Nishida I, Swinhoe R, Slabas AR, and Murata N (1996) Cloning of Brassica napus CTP:phosphocholine cytidylyltransferase cDNAs by complementation in a yeast cct mutant. Plant Mol Biol 31, 205-211.   DOI   ScienceOn
5 Stoutjesdijk PA, Hurlestone C, Singh SP, and Green A (2000) High-oleic acid Australian Brassica napus and B. juncea varieties produced by co-suppression of endogenous Δ12- desaturases. Biochem Soc Trans 28, 938-940.   DOI   ScienceOn
6 Lardizabal KD, Mai JT, Wagner NW, Wyrick A, Voelker T, and Hawkins DJ (2001) DGAT2 is a new diacylglycerol acyltransferase gene family: purification, cloning, and expression in insect cells of two polypeptides from Mortierella ramanniana with diacylglycerol acyltransferase activity. J Biol Chem 276, 38862-38869.   DOI   ScienceOn
7 Broun P, Boddupalli S, and Somerville C (1998) A bifunctionale oleate 12-hydroxylase: desaturase from Lesquerella fendleri. Plant J 13, 201-210   DOI   ScienceOn
8 Brown AP, Coleman J, Tommy AM, Watson MD, and Slabas AR (1994) Isolation and characterization of a maize cDNA that complements a 1-acyl-sn-glycerol-3-P acyltransferase mutant of Escherichia coli and encodes a protein which has similarities to other acyltransferases. Plant Mol Biol 26, 211-223.   DOI   ScienceOn
9 Leonard TW, Lynch J, Mckenna MJ, and Brayden DJ (2006) Promoting absorption of drugs in humans using medium-chain fatty acid-based solid dosage forms: $GIPET^{TM}$. Expert Opinion on Drug Delivery 3, 685-692.   DOI   ScienceOn
10 Kuntzon DS, Hayes TR, Wyrick A, Xiong H, Davies HM, and Voelker TA (1999) Lysophophatidic acid acyltransferase from coconut endosperm mediates the insertion of laurate at the sn- 2 position of triacylglycerols in lauric rapeseed oil and can increase total laurate levels. Plant Physiol 120, 739-746.   DOI   ScienceOn
11 Hirsinger F, and Knowles PF (1984) Morphological and agronomic description of selected Cuphea germplasm. Econ Bot 38, 439- 451.   DOI
12 Zou J, Wei Y, Jako C, Kumar A, Selvaraj G, and Taylor DC (1999) The Arabidopsis thaliana tag1 mutant has a mutation in a diacylglycerol acyltransferase gene. Plant J 19, 645-653.   DOI   ScienceOn
13 Jones A, Davies HM, and Voelker TA (1995) Palmitoyl-acyl carrier protein (ACP) thioesterase and the evolutionary origin of plant acyl-ACP thioesterases. Plant Cell 7, 359-371.   DOI   ScienceOn
14 Gunstone FD, Harwood JL, and Dijkstra AJ (2007) Fatty acid and lipid structure. In The lipid handbook, (3rd ed.). CRC Press, New York, U.S.A.
15 Dehesh K, Jones A, Knutzon DS, and Voelker TA (1996) production of high levels of 8:0 and 10:0 fatty acids in transgenic canola by overexpression of ChFatB2, a thioesterase cDNA from Cuphea hookeriana. Plant J 9, 167-172.   DOI   ScienceOn
16 Dehesh K (2001) How can we genetically engineer oilseed corps to produce high levels of medium-chain fatty acids? Eur J Lipid Sci Technol 103, 688-697.   DOI   ScienceOn
17 Dehesh K, Edwards P, Fillatti J, Slabaugh M, and Byrne J (1998) KAS IV: a 3-ketoacyl-ACP synthase from Cuphea sp. is a medium chain specific condensing enzyme. Plant J 15, 383- 390.   DOI   ScienceOn
18 Wiberg E, Edwards P, Byrne J, Stymme S, and Dehesh K (2000) The distribution of caprylate, caprate and laurate in lipids from developing and mature seeds of transgenic Brassica napus L. Planta 212, 33-40.   DOI   ScienceOn
19 Dahlqvist A, Stahl U, Lenman M, Banas A, Lee M, Sandanger L, Ronne H, and Stymne S (2000) Phospholipid:diacylglycerol acyltransferase: An enzyme that catalyzes the acyl-CoAindependent formation of triacylglycerol in yeast and plants. Proc Natl Acad Sci USA 97, 6487-6492.   DOI   ScienceOn
20 Zheng Z, Zou J (2001) The initial step of the glycerolipid pathway: identification of glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate acyltransferases in Saccharomyces cerevisiae. J Biol Chem 276, 41710-41716.   DOI   ScienceOn
21 Voelker TA, Worrell AC, Anderson L, Bleibaum J, Fan C, Hawkins DJ, Radke SE, and Davies HM (1992) Fatty acid biosynthesis redirected to medium chains in transgenic oilseed plants. Science 257, 72-74.   DOI
22 Voelker TA, Hayes TR, Cranmer AM, Turner JC, and Davies HM (1996) Genetic engineering of a quantitative trait: metabolic and genetic parameters influencing the accumulation of laurate in rapeseed. The Plant J 9, 229-241.   DOI
23 Voelker TA, Jones A, Cranmer AM, Davies HM, and Knutzon DS (1997) Broad-range and binary-range acyl-acyl-carrier protein thioesterases suggest an alternative mechanism for mediumchain production in seeds. Plant Physiol 114, 669-677.   DOI
24 Voelker TA, Kinney AJ (2001) Variations in the biosynthesis of seed-storage lipids. Annu Rev Plant Physiol Plant Mol Biol 52, 335-361.   DOI   ScienceOn
25 Wiberg E, Banas A, and Stymne S (1997) Fatty acid distribution and lipid metabolism in developing seeds of laurate-producing rape (Brassica napus L.) Planta 203, 341-348   DOI   ScienceOn
26 Liu Q, Singh S, and Green A (2000) Genetic modification of cotton seed oil using inverted-repeat gene-silencing techniques. Biochem Soc Trans 28, 927-929.   DOI   ScienceOn
27 Takeuchi H, Sekine S, Kojima K, and Aoyama T (2008) The application of medium-chain fatty acids: edible oil with a suppressing effect on body fat accumulation. Asia Pac J Clin Nutr 17, 320-323.
28 Topfer R, Martini N, and Schell J (1995) Modification of plant lipid synthesis. Science 268, 681-686.   DOI   ScienceOn
29 Ståhl U, Carlson AS, Lenman M, Dahlqvist A, Huang B, Banas W, Banas A, and Stymne S (2004) Cloning and functional characterization of phospholipid:diacylglycerol acyltransferase from Arabidopsis. Plant Physiol 135, 1324-1355.   DOI   ScienceOn
30 Stoll C, Lhs W, Zarhloul MK, and Friedt W (2005) Genetic modification of saturated fatty acids in oilseed rape (Brassica napus). Eur J Lipid Sci Technol 107, 244-248.   DOI   ScienceOn
31 Murphy DJ (2005) Nonfood lipids. In Plant lipids: Biology, Utilization and Manipulation, pp. 103-119. Blackwell Publishing Ltd, Oxford, UK.
32 Liu J-W, Huang Y-S, DeMichele S, Bergana M, Bobik E Jr, Hastilow C, Chuang L-T, Mukerji P, and Knutzon D (2001) Evaluation of the seed oils from a canola plant genetically transformed to produce high levels of $\beta$-linolenic acid. In $\beta$- Linolenic Acid: Recent Advances in Biotechnology and Clinical Applications, Huang Y-S & Ziboh VA (eds.), pp. 61-71. AOCS Press, Champaign, IL, U.S.A