• Journal of Plant Biotechnology
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• 제34권1호
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• pp.75-80
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• 2007

The release of genetically modified organisms ($GMO_{s}$) into the environment has the potential risks regarding the possibility of gene transfer from $GMO_{s}$ to natural organisms and this needs to be evaluated. This study was conducted to monitor the possible horizontal gene transfer from herbicide-resistant zoysiagrass (Zoysia japonica Steud.) to indigenous microorganisms. We have first examined the effect of field-released GM zoysiagrass on the microbial flora in the gut of locust (Locusts mlgratoria). The microbial flora was analyzed through determining the 165 rDHA sequences of microorganisms. The comparison of the microbial flora in the gut of locusts that were captured at the field of GM zoysiagrass and of wild-type revealed that there is no noticeable difference between these two groups. This result indicates that the GM zoysiagrass does not have negative impact on microbial flora in the gut of locust. We then investigated whether the horizontal gene transfer occurred from GM zoysiagrass to microbes in soil, rhizosphere and faecal pellets from locusts by utilizing molecular tools such as Southern hybridization and polymerase chain reaction (PCR). When the total DNAs isolated from microbes in GM zoysiagrass and in wild-type zoysiagrass fields were hybridized with probes for bar or hpt gene, no hybridization signal was detected from both field isolates, while the probes were hybridized with DNA from the positive control. Absence of these genes in the FNAs of soil microorganisms as well as microbes in the gut of locust was further confirmed by PCR. Taken together, our data showed that horizontal gene transfer did not occur in this system. These results further indicate that frequencies of transfer of engineered plant DNA to bacteria are likely to be negligible.

• 대한조선학회지
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• 제25권4호
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• pp.7-12
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• 1988

국제선형시험수조회의의 저항시험에 관한 표준선형인 Series 60, $C_B=0.60$에 대하여 서로 크기가 다른 5척의 모형선을 국내의 선형시험수조 보유기관(한국기계연구소 선박분소, 현대선박해양연구소, 서울대학교, 인하대학교)에서 제작, 교환하여 상사모형시험을 수행하였다. 이 결과를 Hughes 및 Telfer의 방법에 따라 해석하여 형상계수와 모형선의 크기 및 예인속도와의 관계를 파악하고자 했다. 이 선형에 대해서 형상계수는 Reynolds 수 뿐만 아니라 Froude 수에 따라서도 변화하는 것을 확인하였다. 또한 현행의 형상계수를 이용한 실선저항추정법이 공학적 유용성을 가지고 있음을 재확인하였다. 선형특성이 다른 여러 선형에 대해서 상사모형시험을 실시하므로서 보편적인 원리를 파악하는 것이 필요하다고 판단된다.

• 한국농촌간호학회지
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• 제7권1호
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• pp.5-17
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• 2012

Purpose: The objectives of this study were to investigate the prevalence of the metabolic syndrome (MetS) and to identify associated factors with MetS among rural residents. Methods: Data were collected from 1,196 subjects over aged 30 years by a self-administered questionnaire, physical measurement, and blood test in a rural area. The prevalence of MetS was determined by the criteria of the American Heart Association/National Heart, Lung, and Blood Institute (AHA/NHLBI) and 2005 the Korean society for the study of obesity. Results: The prevalence of MetS was 40.5% for men, 49.2% for women. The prevalence of risk factors of MetS was 57.4% for elevated blood pressure, 49.0% for low HDL-cholesterol, and 48.6% for abdominal obesity. Unemployment and higher Body mass index (BMI) were associated factors for MetS regardless of gender. And higher age and physical inactivity in women only increased the odds of the MetS. Especially, BMI was a strong risk factor of MetS in both men and women. Conclusion: The prevalence of metabolic syndrome was higher in a rural area. Therefore, health care providers should develop lifestyle modification program to increase physical activity level and to prevent the obesity among rural residents in order to decrease the prevalence of MetS.

• 생명과학회지
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• 제15권6호
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• pp.941-947
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• 2005

부산 및 경남일대에서 널리 유통되고 있는 생선회, 패류 등의 횟감류 213건을 대상으로 주요 식중독 원인균인 Salmonella spp., Staphylococcus aureus, Vibrio parahaemolyticus, Escherichia coli O157:H7, Bacillus cereus, Listeria monocytogenes 및 Campylobacter jejuni의 오염실태를 조사하였다. V. parahaermolyticus가 65건(30.5%), B. cerus가 21건(9.9%), S. aureus가 8건(3.8%) 및 기타 식중독균이 3건에서 검출되었다. 이를 월별 검출율로 비교해 보면, 7~10월에서 식중독균의 높은 검출율을 나타내었으며, 이러한 높은 검출율은 주로 V. parahaemolyticus에 기인된 것으로 조사되었다. 아울러 생선회에서의 검출율(28.9%) 보다는 패류 등의 기타 횟감류에서 더욱 높은 검출율(49.2%)을 나타내었다. V. parahaemolyticus의 경우 Ampicillin (96.9%), Amikacin (29.2%) 및 Tetracycline (27.7%)에 대해 내성을 나타내었으며, B. rereus의 경우는 Ampicillin (100%), Penicillin G (100%) 및 Rifampicin (71.4%)에 대해 높은 내성을 나타내었다. V. parahaemolyticus와 B. cereus의 생육은 보관온도 및 시간에 크게 영향을 받는 것으로 나타났으며, 수돗물 세척이 해산물에 잔존하는 V. parahaemolyticus의 균수를 크게 감소시키는 것으로 조사되어, 여름철 해산물 섭취로 인한 식중독 발생의 예방을 위해서 $10^{\circ}C$이하의 저온보관과 수돗물로써의 철저한 세척이 필수적인 것으로 판단되었다.

• 대한수의학회지
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• 제39권4호
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• pp.724-729
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• 1999

Amino acids, Na, K and glutathione (GSH) in red blood cells (RBCs) and hematological indices were examined in Korean dogs. A total of seven dogs possessing RBCs with high K and high glutathione (GSH)(HK/HG) were found in 42 Korean dogs : three from Cheju dog, two from Jindo dog and two from Korean mongrel. The RBCs in Korean HK/HG dog contained abnormally high aspartate (Asp), Glu and glutamine (Gln) the same as in HK/HG RBCs from Japanese Shiba dog. Two dogs possessing RBCs with HK and low GSH (HK/LG) were found in Cheju dog, and they accumulated Asp and Gln. Thus, not only the existence of HK dog was confirmed in Korean dogs, but HK/LG dog was also found. The Asp concentration in RBCs from seven of 33 LK dogs was more than $1000{\mu}mol/lc$, the same as in variant LK RBCs with defective Glu/Asp transport (LK/GAT), while it was less than $800{\mu}mol/lc$ in normal LK RBCs. Thus, there were variant dogs having RBCs with abnormally high amino acids accumulation among HK and LK Korean dogs.

• Journal of Preventive Medicine and Public Health
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• 제35권1호
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• pp.1-12
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• 2002

Objective : To estimate the socioeconomic costs of obesity in Korea,1998. Methods : The 1998 National Health and Nutrition Examination Survey(1998 NHNES) data was used and 10,880 persons who had taken health examinations were selected for study. Essential hypertension, NIDDM(non insulin-dependent diabetes mellitus), dyslipidemia, osteoarthritis, coronary heart disease, stroke were included as obesity related disease. The data of direct costs of obesity was obtained from the National Federation of Medical Insurance. The category of indirect costs was the loss of productivity caused by premature death and admission, time costs, traffic costs, nursing fees due to obesity. Multiple logistic regression model was developed to estimate prevalence odds ratio by obesity class adjusted demographic and socio-ecnomic factors and calculate PAF(Population Attributable Fraction) of obesity on obesity related disease. And we finally calculated the socioeconomic costs of obesity in relation to BMI with PAF. Results : The direct costs of obesity were 2,126 billion${\sim}965$ billion Won in considering out of pocket payment to uninsured services, and the indirect costs of obesity were 2,099 billion${\sim}1,086$ billion Won. Consequently, in considering out of pocket payment to uninsured services, the socioeconomic costs of obesity were 4.225 billion${\sim}2,050$ billion Won, which corresponded to about $0.094%{\sim}0.046%$ of GDP and $1.88%{\sim}0.91$ of total health care costs in Korea. Conclusions : Obesity represents a major health problem with significant economic implications for the society. This results are conservative estimates as far as all obesity related disease and all health care and indirect costs were not included due to missing information. further studies are needed to caculate socioeconomic costs of obesity more exactly.

• 대한한의학회지
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• 제26권1호
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• pp.174-186
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• 2005

Objectives : The water extract of Samul-tang (SMT) has traditionally been used for treatment of ischemic heart and brain damage in oriental medicine. However, little is known about the mechanism by which the water extract of SMT rescues cells from these damages. Methods: This study was designed to investigate the protective mechanisms of SMT on oxidative stress-induced toxicity in H9c2 cardiomyoblast cells. Treatment with $H_2O_2$ markedly induced death of H9c2 cardiomyoblast cells in a dose-dependent manner. Results: The characteristics of H20z-induced death of H9c2 showed apparent apoptotic features such as DNA fragmentation and morphological change. However, SMT significantly reduced both H202-induced cell death and morphological change. The decrease of Bc-2 expression by High were inhibited by SMT. In addition, the increase of Bax expression was also inhibited by SMT. The cotreatment of SMT and $H_2O_2$ in H9c2 cells also induced the phosphorylation of ERK in a time-dependent manner. Moreover, PD98059, a specific inhibitor of ERK1/2 attenuated the protective effects of SMT on $H_2O_2-induced$ toxicity in H9c2 cardiomyoblast cells. These results suggest that both ERK1/2 signaling pathways play important roles in the protective effects of SMT on $H_2O_2-induced$ apoptotic death of H9c2 cells. Also, the expression profile of proteins in $H_2O_2$ cardiomyoblast cells were screened by using two-dimensional (2-D) gel electrophoresis. Among 300 spots resolved in 2-D gels, the comparison of control versus apoptosis cells revealed that signal intensity of 17 spots increased and 11 spots decreased. Conclusions: Taken together, this study suggests that the protectiw effects of the water extract of SMT against oxidative damages may be mediated by the modulation of Bc1-2 and Bax expression via the regulation of the ERK signaling pathway.

• Journal of Preventive Medicine and Public Health
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• 제34권4호
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• pp.309-315
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• 2001

Objective : To develop a boner understanding of the relationship between weight status and the prevalence of obesity related diseases in the Korean population. Methods : The 1998 Korean National Health and Nutrition Survey was used and 10,880 persons who had previously taken health examinations were selected for study. The Korean Society for the Study of Obesity's classification of weight status was used. Hypertension, diabetes mellitus, dyslipidemia, osteoarthritis, chronic heart disease, stroke were included as obesity related disease. A logistic regression model was developed to estimate the prevalence odds ratio by obesity class adjusted for demographic and socioeconomic factors and we converted the odds ratio to a prevalence ratio using the base line prevalence of disease to aid in the interpretation of the ratios. Results : The prevalence of obesity was 26.3% based on the KSSO classification $(BMI\geq25)$. A graded increase in the prevalence ratio was observed with increasing severity of overweight and obesity for all health outcomes with the exception of chronic heart disease in men and stroke in both men and women. With normal weight individuals as the reference, for men who were younger than 50 years, the prevalence ratios were highest for hypertension BMI<23-25: 1.70(95% CI=1.41-2.05), 25$BMI\geq30$: 4.83(95% CI=3.70-5.84). The prevalence ratios for dyslipidemia were as high as hypertension, but were lower than hypertension for diabetes mellitus and osteoarthritis. Prevalence ratios generally were greater in younger adults. The prevalence of having 2 or more obesity related diseases increased with weight status category, except in people who were older than 50 years. Conclusions : Based on results, obesity is an increasingly important health problem in Korea and the disease burden increases according to weight status. For Korean adults, the strongest relationship was seen between weight status and hypertension and dyslipidemia. In older people the impact of excess weight and obesity is stronger than that seen in younger people. Increased efforts in the study of obesity and prevention and treatment of obesity and obesity related disease are required.

• 한국광학회지
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• 제24권1호
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• pp.1-8
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• 2013

본 논문에서는 3차원 영상을 위한 LADAR(LAser Detection And Ranging)용 광검출기 모듈을 설계-제작하고 그 특성을 측정한 결과를 보고한다. 광검출기 모듈은 광파이버 어레이와 접속될 수 있도록 200 um 직경을 갖는 InGaAs APD(Avalanche Photodiode)로 설계-제작하였으며, 선형모드 동작 특성을 만족하도록 TIA(Trans-impedance Amplifier)를 설계-제작하였다. 광검출기 모듈을 구성하는 핵심부품들은 12개의 lead pin을 갖는 TO8 상에 집적되었으며, 집적에 필요한 APD 서브마운트 및 TIA 회로 등을 자체적으로 설계-제작하여 사용하였다. 제작한 광검출기 모듈은 450 ps의 rising time과 780 MHz의 대역폭 특성을 보였으며, 0.8 mV 이하의 잡음 특성과, 150 nW의 MDS(Minimum Detectable Signal) 신호 크기에 대해 15 이상의 신호대 잡음비(SNR)를 보임으로써 설계한 모든 특성을 만족하였는데, 이는 저자들이 아는 한 200 um 직경의 대면적 InGaAs APD를 이용한 광수신기에서 가장 우수한 특성을 나타낸 것이다.

• Journal of Preventive Medicine and Public Health
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• 제37권1호
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• pp.44-50
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• 2004

Objectives : This study was conducted in order to investigate the professionalism, job stress and job satisfaction in western and oriental doctors in Korea. Methods : The authors conducted a survey using a self-administered questionnaire, conducted between August and September, 2001. The study subjects were 457 western doctors and 161 oriental doctors practicing at local clinics in Daegu City. Results : For the professionalism scale, the score for belief in service to the public of western doctors was significantly higher than that of oriental doctors (p<0.05) whereas the scores for sense of calling to the field and feeling of autonomy were higher in oriental doctors (p<0.01). For the job stress scale, the scores for work factor, and clinical responsibility/decision factor of western doctors were significantly higher than those of oriental doctors (p<0.01). Of the western doctors, 59.7% expressed satisfaction with their job as a doctor, 69.5% responded that the role of a doctor was appropriate to their aptitude, and 61.8% answered that they wouldn't consider other kinds of job even if offered. In case of oriental doctors, these responses were 83.1%, 82.5%, and 82.3%, respectively. Conclusions : The job stress scores of western doctors were higher than those of oriental doctors, but the job satisfaction was lower. The reasons for the above results are not clear. Further studies are required to understand the characteristics of job stress and job satisfaction in doctors.