• Korean Journal of Pharmacognosy
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• v.45 no.1
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• pp.48-54
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• 2014

Alzheimer's disease is progressive neurodegenerative disorder by the loss of memory and learning abilities. Codonopsis lanceolata (C. lanceolata) is traditional medicinal plant used for the treatment of inflammatory diseases. The aim of study was to evaluate the effect of steamed C. lanceolata on scopolamine-induced memory impairment in the Morris water maze test and passive avoidance test. In addition, this study investigated the neuroprotective effects of steamed C. lanceolata on glutamate-induced cell death in HT22 cells using MTT assay. The results showed that steamed C. lanceolata (500 mg/kg body weight, p.o.) reversed spatial memory impairment by scopolamine in Morris water maze test and passive avoidance test. Steamed C. lanceolata attenuated memory impairment by scopolamine compared with common C. lanceolata. In addition, administration of steamed C. lanceolata significantly also reduced cell death. We suggest that steaming process more improve cognitive enhancing and neuroprotective effect of C. lanceolata than common C. lanceolata.

• Korean Journal of Optics and Photonics
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• v.21 no.6
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• pp.230-234
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• 2010

Highly-Birefringent Photonic Crystal Fiber (Hi-Bi PCF) is composed of a single material, silica, so that its temperature sensitivity is extremely low. Therefore, we propose afiber based Sagnac interferometer for measurement of strain and curvature independent of temperature variation. The sensitivities of strain and curvature (both axes) are measured to be $1.41\;pm/{\mu}{\varepsilon}$ and $0.93nm/m^{-1}$(slow axis Y), $-1.6\;nm/m^{-1}$(fast axis X), respectively.

• Korean Journal of Optics and Photonics
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• v.21 no.6
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• pp.235-240
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• 2010

We fabricate highly birefringent photonic crystal fiber with new structure using a stack and draw method. Fabricated fiber has two big air holes, one at each side of the outside air cladding region, leading to core ellipticity during the drawing process. Birefringence of the fabricated Hi-Bi PCF is measured to be $2.29{\times}10^{-4}$ (at 1550 nm).

• Korean Journal of Pharmacognosy
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• v.44 no.1
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• pp.53-59
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• 2013

This study was conducted to determine the content of atractylenolides I and III in Atractylodes japonica. An established HPLC-DAD method was used to monitor contents of atractylenolides I and III in A. japonica obtained from Korea and China and compared with contents of A. macrocephala, A. chinensis, and A. lancea. Quantitative analysis of atractylenolides I and III was carried out on a Shiseido C18 column (S-5 ${\mu}m$, 4.6 mm I.D. ${\times}$ 250 mm) with gradient elution composed of acetonitile-water. The results show that the average content of atractylenolides I and III in A. japonica were 0.0954 and 0.1963%, respectively, and contents of A. lancea were higher than A. macrocephala, A. chinensis. In this study, we identified the differentiation of the quality of A. japonica from different species and collected locations and established content standard of atractylenolides I and III in A. japonica and this content standard was helpful to quality control of A. japonica.

• Natural Product Sciences
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• v.19 no.1
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• pp.20-27
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• 2013

A simple high performance liquid chromatography - diode array detector (HPLC-DAD) method has been developed and validated for simultaneous determination of the six components (cinnamic acid, cinnamaldehyde, eugenol, atractylenolide I, atractylenolide III, and ergosterol) in Oryeongsan. In addition, identification of six marker compounds was conducted by a LC-MS/MS analysis. The six compounds in Oryeongsan were separated on Shishedo $C_{18}$ column (5 ${\mu}m$, $4.6{\times}250$ mm) at a column temperature of $30^{\circ}C$. The mobile phase was a mixture of 0.1% trifluoroacetic acid (TFA) water and acetonitrile employing gradient elution at a flow rate of 1.0 mL/min. The detection wavelength was set at 205, 250, 280, and 330 nm. The developed method had good linearity ($R^2$ > 0.9997) and the limit of detection (LOD) and limit of quantification (LOQ) were observed within the ranges 0.01~0.15 and 0.05~0.45 ${\mu}g/mL$, respectively. The relative standard deviation (RSD) values of intra- and inter-day testing were indicated that less than 3% and 90.31~103.31% of accuracy. The results of recovery test were 90.56~106.72% and RSD range was measured from 0.84 to 2.95%. In conclusion, this HPLC-DAD method has been successfully applied to the simultaneous determination of six compounds in Oryeongsan samples.

• Journal of Korean Society of Transportation
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• v.29 no.1
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• pp.113-123
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• 2011

The application of micro-simulation model has been extended farther with improvement of computer performance and development of complicated model. To make a micro-simulation model accurately replicate field traffic conditions, model calibration is very crucial. Studies on calibration of micro-simulation model have not been enough while lots of studies on calibration of macro-simulation model have been continued in our country. This paper presents an auto-calibration of parameter values in micro-simulation model(VISSIM) using genetic algorithm. RMSE(Root Mean Square Error) of collected volume on the urban expressway versus simulated volume is set as MOP(measure of performance) and objective function of optimization is set as to minimize the RMSE. Applying to urban expressway(Nae-bu circular) as a case study, it shows that RMSE of optimized parameter values decrease 60.4%($19.3{\longrightarrow}7.6$) compared to default parameter values and the proposed auto-calibration system is very effective.

• Biomolecules & Therapeutics
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• v.22 no.3
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• pp.246-253
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• 2014

Codonopsis lanceolata has been used as an herbal medicine for several lung inflammatory diseases, such as asthma, tonsillitis, and pharyngitis. Previously, we showed the neuroprotective effect of steamed and fermented C. lanceolata (SFC) in vitro and in vivo. In the current study, the treatment of HT22 cells with SFC decreased glutamate-induced cell death, suggesting that SFC protected HT22 cells from glutamate-induced cytotoxicity. Based on these, we sought to elucidate the mechanisms of the neuroprotective effect of SFC by measuring the oxidative stress parameters and the expression of Bax and caspase-3 in HT22 cells. SFC reduced contents of ROS, $Ca^{2+}$ and NO. Moreover, SFC restored contents of glutathione and glutathione reductase as well as inhibited Bax and caspase-3 activity in HT22 cells. These results indicate that steamed and fermented C. lanceolata (SFC) extract protected HT22 cells by anti-oxidative effect and inhibition of the expression of Bax and caspase-3.

• Journal of Sasang Constitutional Medicine
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• v.18 no.3
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• pp.185-194
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• 2006

1. Objecrives Although Lee Je-Ma emphasized it is important to be aware of our own Nature & Emotion(性情) and control them properly, there is few case report about Taeyangin focused on their psychological sides. This report is about a Taeyangin patient who had Hae Yuk and Yul Gyuk syndrome caused by her stress from the work place and relationship with her seniors 2. Methods We observed the female patient had hospitalized in the Oriental Hospital of Dongguk University more than twice has been followed up for the last one year. We prescripted Mi-hoo-teung-shik-chang Tang as her physical symptom. The improvement of her physical conditions were checked through her sleep, meal, menstruation, stool and urine. And MMPI has been tested three times for the last one year to analyze her psychological progress. 3. Results and Conclusions Working in a group can cause hard stress for Taeyangin who is not good at adjusting themselves to whatever environment they are in. Mi-hoo-teung-shik-chang Tang could improve Taeyangin's physical conditions such as Hae Yuk, Yul Gyuk, insomnia, nausea, anorexia and hypouresis. But as her psychological aspect, she was getting more anxious, depressed and avoiding contact with others due to the stress. It is necessary to rake care of patients' psychological sides as well as physical conditions through managing their Nature & Emotion(性情).

• Journal of Physiology & Pathology in Korean Medicine
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• v.21 no.4
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• pp.961-966
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• 2007

Lesser yang person-Hyungbangpaedok-san (HBPDS) is a prescription originated in the

• YAKHAK HOEJI
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• v.58 no.5
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• pp.287-293
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• 2014

Alzheimer's disease (AD), most common form of dementia is characterized that memory deficit and loss of cognitive function. This study was evaluated cognitive enhancing effect of water and ethanol extracts of Codonopsis lanceolata and compared using Morris water maze and passive avoidance test. The water and 70% ethanol extracts (100, 300 and 500 mg/kg) were administered to mice. The neuroprotective effect on glutamate-induced cell death in HT22 cells was additionally investigated using MTT assay. Results showed 70% ethanol extract of Codonopsis lanceolata enhanced cognitive function than water extract, as shown by decrease in escape latency time in Morris water maze test. In passive avoidance test, 70% ethanol extract also increased the latency time compared to the water extract. Furthermore, 70% ethanol extract significantly protected neuronal cell against glutamate cytotoxicity and showed higher than neuroprotective effect of water extract. These results indicate that 70% ethanol extract more improve spatial cognitive ability and protected neuronal cells than water extract.