Quantitative Analysis of Atractylenolides I and III in Atractylodes japonica

백출의 Atractylenolide I과 Atractylenolide III의 함량분석

  • Yun, Bo-Ra (Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, Kangwon National University) ;
  • Weon, Jin Bae (Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, Kangwon National University) ;
  • Lee, Bohyoung (Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, Kangwon National University) ;
  • Lee, Jiwoo (Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, Kangwon National University) ;
  • Eom, Min Rye (Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, Kangwon National University) ;
  • Ma, Choong Je (Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, Kangwon National University)
  • 윤보라 (강원대학교 의생명소재공학전공) ;
  • 원진배 (강원대학교 의생명소재공학전공) ;
  • 이보형 (강원대학교 의생명소재공학전공) ;
  • 이지우 (강원대학교 의생명소재공학전공) ;
  • 엄민례 (강원대학교 의생명소재공학전공) ;
  • 마충제 (강원대학교 의생명소재공학전공)
  • Received : 2013.01.11
  • Accepted : 2013.02.07
  • Published : 2013.03.31

Abstract

This study was conducted to determine the content of atractylenolides I and III in Atractylodes japonica. An established HPLC-DAD method was used to monitor contents of atractylenolides I and III in A. japonica obtained from Korea and China and compared with contents of A. macrocephala, A. chinensis, and A. lancea. Quantitative analysis of atractylenolides I and III was carried out on a Shiseido C18 column (S-5 ${\mu}m$, 4.6 mm I.D. ${\times}$ 250 mm) with gradient elution composed of acetonitile-water. The results show that the average content of atractylenolides I and III in A. japonica were 0.0954 and 0.1963%, respectively, and contents of A. lancea were higher than A. macrocephala, A. chinensis. In this study, we identified the differentiation of the quality of A. japonica from different species and collected locations and established content standard of atractylenolides I and III in A. japonica and this content standard was helpful to quality control of A. japonica.

Keywords

References

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