• Korean Journal of Pharmacognosy
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• v.46 no.1
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• pp.17-22
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• 2015

Previous phytochemical studies of Vigna angularis (Ohwi) Ohwi & Ohashi (Leguminosae) have shown the presence of saponins and flavonoids. From the seed of V. angularis, genistein-7-O-${\beta}$-D-glucopyranoside (genistin) was isolated. Lifespan-extending effect of genistin was elucidated using Caenorhabditis elegans model system. Genistin showed potent lifespan extension of worms under normal culture condition. This compound also exhibited the protective effects against thermal and oxidative stress conditions. In the case of heat stress, genistin-treated worms exhibited enhanced survival rate, compared to control worms. In addition, genistin-fed worms lived longer than control worms under oxidative stress induced by paraquat. To verify the possible mechanism of genistin-mediated increased lifespan and stress resistance of worms, we investigated whether genistin might alter superoxide dismutase (SOD), catalase activities and intracellular ROS levels. Our results showed that genistin was able to elevate SOD and catalase activities of worms and reduce intracellular ROS accumulation in a dose-dependent manner.

• Journal of Korean Medicine Rehabilitation
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• v.30 no.2
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• pp.37-46
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• 2020

Objectives In this study, we analyzed the effect of Dioscoreae Rhizoma on exercise practice ability and blood fatigue variable factor in the forced swimming test. Methods Male mice were divided into four group. Mice were administrated with saline, Discorae Rhizoma extracted by water (DRW), Discorae Rhizoma extracted by 30% ethanol (DRE), red ginseng (RG), orally once per day, for 1 week. Exercise performance was evaluated by exhaustive swimming time and weights. Glycogen contents of liver and soleus muscle were measured at the end of the experiment. In addition, the biochemical parameters such as asparate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen, creatine, lactate dehydrogenase (LDH), ammonia, triglycerides (TG), total cholesterol (TC) and free fatty acid (FFA) were analyzed. Results Both DRW and DRE showed an improvement of swimming endurance compared with control group. Levels of ALT, ammonia, TG, TC and FFA were significantly decreased in both DRW and DRE compared with control group. Levels of AST, Creatine were significantly reduced in DRE group compared with control group. Moreover LDH showed only a tendency to increase in the DRW and DRE groups. Conclusions In summary, the present study showed that Dioscoreae Rhizoma improved in physical fatigue and exercise practice ability and DRE improved the superior than those of DRW.

• Korean Journal of Pharmacognosy
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• v.44 no.3
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• pp.230-234
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• 2013

In the course of screening for antioxidant compounds by measuring the DPPH radical scavenging activity, a total extract of the aerial part of Trigonotis peduncularis (Boraginaceae) was found to show potent DPPH radical scavenging activity. Subsequent activity-guided fractionation of the methanolic extract of T. peduncularis led to the isolation of seven compounds, kaempferol (1), quercetin (2), trigonotin A (3), astragalin (4), trigonotin C (5), kaempferol-3-O-${\alpha}$-L-rhamnopyranosyl(1${\rightarrow}$6)-${\beta}$-D-glucopyranoside (6), and rutin (7) from the active ethyl acetate soluble fraction. Their structures were elucidated by spectroscopic studies. Among seven compounds, compounds 1, 2, 4, 6 and 7 were isolated for the first time from this plant, and compounds 2 and 7 showed the significant DPPH free radical scavenging activities.

• BMB Reports
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• v.54 no.7
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• pp.380-385
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• 2021

Proper targeting of the βPAK-interacting exchange factor (βPIX)/G protein-coupled receptor kinase-interacting target protein (GIT) complex into distinct cellular compartments is essential for its diverse functions including neurite extension and synaptogenesis. However, the mechanism for translocation of this complex is still unknown. In the present study, we reported that the conventional kinesin, called kinesin-1, can transport the βPIX/GIT complex. Additionally, βPIX bind to KIF5A, a neuronal isoform of kinesin-1 heavy chain, but not KIF1 and KIF3. Mapping analysis revealed that the tail of KIF5s and LZ domain of βPIX were the respective binding domains. Silencing KIF5A or the expression of a variety of mutant forms of KIF5A inhibited βPIX targeting the neurite tips in PC12 cells. Furthermore, truncated mutants of βPIX without LZ domain did not interact with KIF5A, and were unable to target the neurite tips in PC12 cells. These results defined kinesin-1 as a motor protein of βPIX, and may provide new insights into βPIX/GIT complex-dependent neuronal pathophysiology.

• Korean Journal of Pharmacognosy
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• v.52 no.3
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• pp.170-176
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• 2021

Anthocyanins are considered as one of the major functional components including cyanidin-3-glucoside and peonidin-3-glucoside anthocyanins of black rice (Oryza sativa L., BR) aleurone layer (OAL) extract. The immunomodulatory biological effects of OAL has rarely been evaluated. This study has extensively studied the immunomodulatory effects of BR in immunosuppressed mice by CPA. The immunostimulatory effects of BR extract in in vivo at 150 (OAL-1) and 300 mg/kg body weight (OAL-2) for OAL groups were measured and compared to the normal group (CON) ingested with distilled water only or other groups. After the 14 days of oral administration, immune related organ weights, serum immunoglobulin (Ig) A and IgG, and cytokines (IL-12, TNF-α) levels, splenocytes proliferation rate, and NK cell activity were evaluated as immune related biomarkers. The serum IgA and IgG values in the OAL-treated groups increased to the level of the CON. OAL significantly and effectively controlled the IL-12 and TNF-α levels and NK activity compared to the negative control (NC) group. The results suggest that BR aleurone layer with immunomodulatory activities has a great potential as a functional food by itself or as a supplement.

• The Journal of Churna Manual Medicine for Spine and Nerves
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• v.16 no.1
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• pp.13-23
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• 2021

Objectives To assess the effectiveness of chuna manual therapy for patients with total knee arthroplasty. Methods We searched 9 electronic databases(KISS, NDSL, RISS, OASIS, KMBASE, Cochrane Library, PubMed, CNKI, Wangfang data) using the keywords 'total knee arthroplasty OR total knee replacement' and 'tuina OR chuna' to identify RCTs that investigated the effectiveness of Chuna manual therapy after total knee arthroplasty. Results 23 RCTs were selected based on inclusion criteria. The systematic review showed positive effect of using chuna manual therapy after total knee arthroplasty. Conclusions 23 studies reported that chuna manual therapy had significant effects on total knee arthroplasty. However, many studies were exposed to probable high risk of bias. Also, all of the studies were published in one overseas country. Therefore, further research is required using well-designed RCTs to support the effectiveness of chuna manual therapy.

• The Journal of Churna Manual Medicine for Spine and Nerves
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• v.16 no.1
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• pp.43-52
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• 2021

Objectives The purpose of this study was to investigate the effectiveness of Chuna manual therapy (CMT) in the treatment of simple obesity. Methods We used seven databases to perform a literature search using the keywords "Chuna OR Tuina" AND "Obesity." We selected randomized clinical trials in which Chuna was used as the main method for treating simple obesity. The risk of bias was assessed by three independent researchers using the Cochrane risk of bias. Results A total of 14 RCTs were selected and analyzed. The results of the meta-analysis showed a significantly higher efficacy rate in the patient group treated with CMT as the main treatment than that in the control group. Conclusions The results of this systematic review suggest that CMT is effective in the treatment of simple obesity. However, given the limitations such as the small number of studies and the high risk of bias in the studies, additional research is needed to lend further support to this claim.

• Journal of Korean Medicine Rehabilitation
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• v.31 no.1
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• pp.165-173
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• 2021

Objectives The objective of this study is to propose postoperative rehabilitation for spondylolisthesis after posterior lumbar interbody fusion (PLIF) in Korean medicine and to report its effectiveness. Methods There were two patients who were received Korean medicine with acupuncture, herbal medicine, cupping and exercise treatment after PLIF. Patients were evaluated numeric rating scale (NRS), Oswestry disability index (ODI), pain free walking distance (PFWD), Korean modified index (K-MBI), EuroQol-5 dimension (EQ-5D) index. Results Case 1 was improved NRS from 7 to 3, Case 2 was improved NRS from 7 to 2. Also, ODI, PFWD, K-MBI, EQ-5D score were improved in both cases. Conclusions This study suggests that Korean medicine rehabilitation could be effective for spondylolisthesis after PLIF.

• Knowledge Management Research
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• v.22 no.4
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• pp.1-26
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• 2021

Measuring the intangible asset has been vigorously studied for its importance. Especially, the value of character in media industry is difficult to quantitatively evaluate in spite of the industry's rapid growth. Recently, the Social Network Analysis (i.e., SNA) has been actively applied to understand human usage patterns in a media field. By using SNA methodology, this study attempts to investigate how the character network characteristics of media works are linked to human search behaviors. Our analysis reveals the positive correlation and causality between character network centralities and character search data. This result implies that the character network can be used as a clue for the valuation of character assets.

• Korean Journal of Acupuncture
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• v.38 no.4
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• pp.275-281
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• 2021

Objectives : This study reports changes in liver function test (LFT) after Korean Medicine treatment in patients admitted to Korean Medicine hospital with abnormal LFT. The purpose of this study is to investigate the relationship between the Korean medicine treatment and abonormal LFT to verify safety of Korean medicine treatment by analyzing index of LFT. Methods : From Oct. 2015 to Sep. 2020, the result was analyzed for 91 patients admitted to the Pohang Korean Medicine Hospital and received Korean Medicine treatment. Asparate aminotransferase (AST), alanine aminotransferase (ALT) and total bilirubin (TB) were compared at admission and discharge. Results : Comparison between admission and discharge LFT showend AST decreased from 52.72±25.08 to 43.2±19.20, ALT from 70.85±32.40 to 62.13±29.40, and TB from 1.33±0.37 to 0.81±0.29. Conclusions : After Korean medicine treatment, AST, ALT, and TB decreased compared with the values at admission. Further studies on safety of Korean Medicine treatment are warranted.