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http://dx.doi.org/10.22889/KJP.2021.52.3.170

Effects of Black rice (Oryza sativa L.) Aleurone layer on Cyclophosphamide-induced Immunosuppression in Mice  

Lee, Eun Byeol (National Institute of Agricultural Sciences, Rural Development Administration)
Choi, Ji-Hye (National Institute of Agricultural Sciences, Rural Development Administration)
Kim, Si Hyun (National Institute of Agricultural Sciences, Rural Development Administration)
Jang, Hwan Hee (National Institute of Agricultural Sciences, Rural Development Administration)
Lee, Sung Hyen (National Institute of Agricultural Sciences, Rural Development Administration)
Publication Information
Korean Journal of Pharmacognosy / v.52, no.3, 2021 , pp. 170-176 More about this Journal
Abstract
Anthocyanins are considered as one of the major functional components including cyanidin-3-glucoside and peonidin-3-glucoside anthocyanins of black rice (Oryza sativa L., BR) aleurone layer (OAL) extract. The immunomodulatory biological effects of OAL has rarely been evaluated. This study has extensively studied the immunomodulatory effects of BR in immunosuppressed mice by CPA. The immunostimulatory effects of BR extract in in vivo at 150 (OAL-1) and 300 mg/kg body weight (OAL-2) for OAL groups were measured and compared to the normal group (CON) ingested with distilled water only or other groups. After the 14 days of oral administration, immune related organ weights, serum immunoglobulin (Ig) A and IgG, and cytokines (IL-12, TNF-α) levels, splenocytes proliferation rate, and NK cell activity were evaluated as immune related biomarkers. The serum IgA and IgG values in the OAL-treated groups increased to the level of the CON. OAL significantly and effectively controlled the IL-12 and TNF-α levels and NK activity compared to the negative control (NC) group. The results suggest that BR aleurone layer with immunomodulatory activities has a great potential as a functional food by itself or as a supplement.
Keywords
Black rice; Aleurone layer; Immune; Cyclophosphamide; NK activity;
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1 Choi, B. K., Nam, S. Y., Lee, Y. M., Kim, J. B., Choe, J. S., Lee, H. J., Lee, S. J. and Lee, S. H. (2015) Supplementary effects of black rice (Oryza sativa L.) Aleurone layer extract on body fat, serum lipid, and serum hormone levels in ovariectomized rats. The Journal of the Korea Contents Association 15: 599-605.   DOI
2 Lee, E. B., Lee, S. H., Park, Y. G., Choi, J. H., Lee, H. K., Jang, H. H., Hwang, K. A., Choe, J. S., Park, S. Y., Choi, A. J., Hwnag, I. G., Kim, J. S., Lee, H. J., Lee, S. J. and Jeong, H. C. (2019) Platycodon grandiflorum extract ameliorates cyclophosphamide-induced immunosuppression in mice. J. East Asian Soc. Diet. Life 29: 303-309.   DOI
3 Chen, S., Liu, C., Huang, X., Hu, L., Huang, Y., Chen, H., Fang, Q., Dong, N., Li, M., Tang, W. and Nie, S. (2020) Comparison of immunomodulatory effects of three polysaccharide fractions from Lentinula edodes water extracts. J. Funct. Foods 66: 103791.   DOI
4 Byun, E. H. (2015) Comparison study of immunomodulatory activity of polysaccharide and ethanol extracted from Sargassum fulvellum. J. Korean Soc. Food Sci. Nutr. 44: 1621-1628.   DOI
5 Yang, L. C., Hsieh, C. C. and Lin, W. C. (2015) Characterization and immunomodulatory activity of rice hull polysaccharides. Carbohydr. Polym. 124: 150-156.   DOI
6 Choi, J. P., Kim, Y. S., Kim, O. Y., Kim, Y. M., Jeon, S. G., Roh, T. Y., Park, Y. S. and Kim, Y. K. (2012) TNF-alpha is a key mediator in the development of T h2 cell response to inhaled allergens induced by a viral PAMP double-stranded RNA. Allergy 67: 1138-1148.   DOI
7 Hussain, A., Shadma, W., Maksood, A. and Ansari, S. H. (2013) Protective effects of Picrorhiza kurroa on cyclophosphamide-induced immunosuppression in mice. Pharmacognosy Res. 5: 30.   DOI
8 Cerutti, A., Chen, K. and Chorny, A. (2011). Immunoglobulin responses at the mucosal interface. Annu. Rev. Immunol. 29: 273-293.   DOI
9 Yoo, H. J., You, D. J. and Lee, K. W. (2019) Characterization and immunomodulatory effects of high molecular weight fucoidan fraction from the sporophyll of Undaria pinnatifida in cyclophosphamide-induced immunosuppressed mice. Mar. drugs 17: 447.   DOI
10 Wang, Y., Qi, Q., Li, A., Yang, M., Huang, W., Xu, H., Zhao, Z. and Li, S. (2016) Immuno-enhancement effects of Yifei Tongluo Granules on cyclophosphamide-induced immunosuppression in Balb/c mice. J. Ethnopharmacol. 194: 72-82.   DOI
11 Shirani, K., Hassani, F. V., Razavi-Azarkhiavi, K., Heidari, S., Zanjani, B. R. and Karimi, G. (2015) Phytotrapy of cyclophosphamide-induced immunosuppression. Environ. Toxicol. Pharmacol. 39: 1262-1275.   DOI
12 Dowell, A. C., Oldham, K. A., Bhatt, R. I., Lee, S. P. and Searle, P. F. (2012) Long-term proliferation of functional human NK cells, with conversion of CD56 dim NK cells to a CD56 bright phenotype, induced by carcinoma cells coexpressing 4-1BBL and IL-12. Cancer Immunol. Immunother. 61: 615-628.   DOI
13 Yu, F., Zhang, Z., Ye, S., Hong, X., Jin, H., Huang, F., Yang, Z., Tang, Y., Chen, Y. and Ding, G. (2019) Immunoenhancement effects of pentadecapeptide derived from Cyclina sinensis on immune-deficient mice induced by Cyclophosphamide. J. Funct. Foods 60: 103408.   DOI
14 Gonzalez-Rey, E., Chorny, A. and Delgado, M. (2007) Regulation of immune tolerance by anti-inflammatory neuropeptides. Nat. Rev. Immunol. 7: 52-63.   DOI
15 World Health Organization. (2020). International guidelines for certification and classification (coding) of COVID-19 as cause of death. International Statistical Classification of Diseases.
16 Sujono, T. A., Kusumowati, I. T. D. and Munawaroh, R. (2021) Effects of Jamaican cherry (Muntingia calabura L.) Fruits Extract on Immunoglobulin G Levels and Hematological Profiles in Mice. Pharmacogn. J. 13: 535-541.
17 Ilangkovan, M., Jantan, I., Mesaik, M. A. and Bukhari, S. N. A. (2015) Immunosuppressive effects of the standardized extract of Phyllanthus amarus on cellular immune responses in Wistar-Kyoto rats. Drug Des. Devel. Ther. 9: 4917.   DOI
18 Cheng, D., Wan, Z., Zhang, X., Li, J., Li, H. and Wang, C. (2017) Dietary Chlorella vulgaris ameliorates altered immunomodulatory functions in cyclophosphamide-induced immunosuppressive mice. Nutrients. 9: 708.   DOI
19 Lee, S. B., Cha, J., Kim, I. K., Yoon, J. C., Lee, H. J., Park, S. W., Cho, S. J., Youn, D. Y., Lee, H. J., Lee, C. H., Lee, J. M., Lee, K. Y. and Kim, J. S. (2014) A high-throughput assay of NK cell activity in whole blood and its clinical application. Biochem. Biophys. Res. Commun. 445: 584-590.   DOI
20 Yu, Y., Song, Q., Huang, L., Shen, M., Yu, Q., Chen, Y. and Xie, J. (2020) Immunomodulatory activities of sulfated Cyclocarya paliurus polysaccharides with different degrees of substitution on mouse spleen lymphocytes. J. Funct. Foods 64: 103706.   DOI
21 Zhang, M. W., Zhang, R. F., Zhang, F. X. and Liu, R. H. (2010) Phenolic profiles and antioxidant activity of black rice bran of different commercially available varieties. J. Agric. Food Chem. 58: 7580-7587.   DOI
22 Zhou, Y., Chen, X., Yi, R., Li, G., Sun, P., Qian, Y. and Zhao, X. (2018) Immunomodulatory effect of tremella polysaccharides against cyclophosphamide-induced immunosuppression in mice. Molecules 23: 239.   DOI
23 Korean Statistical Information Service. (2020) Food grain consumption survey. Statistics Korea.
24 Lee, H. M., Im, J. S., Park, J. D., Kum, J. S., Lee, H. Y. and Lee, Y. T. (2013) Amylolytic activity of brown rice and black rice during germination. J. Food Sci. Technol. 45: 333-338.
25 Kong, S., Kim, D. J., Oh, S. K., Choi, I. S., Jeong, H. S. and Lee, J. (2012) Black rice bran as an ingredient in noodles: chemical and functional evaluation. J. Food Sci. 77: C303-C307.   DOI
26 Kim, H. J., Park, H. Y., Kim, M. Y., Lee, J. Y., Lee, J. H., Lee, J. Y., Lee, B. W., Kim, M. and Lee, B. (2021) Physicochemical characteristics of brown rice porridge added with colored rice cultivars. JKFN 50: 279-284.   DOI
27 Min, S. W., Ryu, S. N. and Kim, D. H. (2010) Anti-inflammatory effects of black rice, cyanidin-3-O-β-D-glycoside, and its metabolites, cyanidin and protocatechuic acid. Int. Immunopharmacol. 10: 959-966.   DOI
28 Chen, P. N., Kuo, W. H., Chiang, C. L., Chiou, H. L., Hsieh, Y. S. and Chu, S. C. (2006) Black rice anthocyanins inhibit cancer cells invasion via repressions of MMPs and u-PA expression. Chem. Biol. Interact. 163: 218-229.   DOI
29 Min, B., McClung, A. M. and Chen, M. H. (2011) Phytochemicals and antioxidant capacities in rice brans of different color. J. Food Sci. 76: C117-C126.   DOI
30 Martin-F., a, A., Thomsen, L. L., Brett, S., Gerard, C., Lipp, M., Lanzavecchia, A. and Sallusto, F. (2004) Induced recruitment of NK cells to lymph nodes provides IFN-γ for TH 1 priming. Nat. Immunol. 5: 1260-1265.   DOI
31 Lee, M. K., Ryu, S. I. and Lee, M. H. (2018) Improvement of anti-oxidant and anti-inflammatory activities of aleurone layer extracts of black rice (Oryza sativa L.) by enzyme treatment. J. Food. Sci. Technol. 50: 528-534.   DOI
32 Wang, H., Xu, L., Yu, M., Wang, Y., Jiang, T., Yang, S. and Lv, Z. (2019) Glycosaminoglycan from Apostichopus japonicus induces immunomodulatory activity in cyclophosphamide-treated mice and in macrophages. Int. J. Biol. Macromol. 130: 229-237.   DOI
33 Shin, H. Y., Kim, S. M., Lee, J. H. and Lim, S. T. (2019) Solid-state fermentation of black rice bran with Aspergillus awamori and Aspergillus oryzae: Effects on phenolic acid composition and antioxidant activity of bran extracts. Food Chem. 272: 235-241.   DOI
34 Marieb, E. N. (1998) Human anatomy and Physiology, Fourth edition, Benjamin / Cummings Publishing Co. California. 612-617, 723-725.