• Fisheries and Aquatic Sciences
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• v.20 no.12
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• pp.31.1-31.11
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• 2017

Background: Liver-expressed antimicrobial peptide-2 (LEAP-2) is an important component of innate immune system in teleosts. In order to understand isoform-specific involvement and regulation of LEAP-2 genes in mud loach (Misgurnus mizolepis, Cypriniformes), a commercially important food fish, this study was aimed to characterize gene structure and expression characteristics of two paralog LEAP-2 isoforms. Results: Mud loach LEAP-2 isoforms (LEAP-2A and LEAP-2B) showed conserved features in the core structure of mature peptides characterized by four Cys residues to form two disulfide bonds. The two paralog isoforms represented a tripartite genomic organization, known as a common structure of vertebrate LEAP-2 genes. Bioinformatic analysis predicted various transcription factor binding motifs in the 5'-flanking regions of mud loach LEAP-2 genes with regard to development and immune response. Mud loach LEAP-2A and LEAP-2B isoforms exhibited different tissue expression patterns and were developmentally regulated. Both isoforms are rapidly modulated toward upregulation during bacterial challenge in an isoform and/or tissue-dependent fashion. Conclusion: Both LEAP-2 isoforms play protective roles not only in embryonic and larval development but also in early immune response to bacterial invasion in mud loach. The regulation pattern of the two isoform genes under basal and stimulated conditions would be isoform-specific, suggestive of a certain degree of functional divergence between isoforms in innate immune system in this species.

• The Korean Journal of Nuclear Medicine Technology
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• v.13 no.3
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• pp.102-109
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• 2009

Purpose: Flash 3D (pixon(R) method; 3D OSEM) was developed as a software program to shorten exam time and improve image quality through reconstruction, it is an image processing method that usefully be applied to nuclear medicine tomography. If perfoming brain diamox perfusion scan by reconstructing subtracted images by Flash 3D with shortened image acquisition time, there was a problem that SNR of subtracted image is lower than basal image. To increase SNR of subtracted image, we use LEAP collimators, and we emphasized on sensitivity of vessel dilatation than resolution of brain vessel. In this study, our purpose is to confirm possibility of application of LEAP collimators at brain diamox perfusion tomography, identify proper reconstruction factors by using Flash 3D. Materials and methods: (1) The evaluation of phantom: We used Hoffman 3D Brain Phantom with $^{99m}Tc$. We obtained images by LEAP and LEHR collimators (diamox image) and after 6 hours (the half life of $^{99m}Tc$: 6 hours), we use obtained second image (basal image) by same method. Also, we acquired SNR and ratio of white matters/gray matters of each basal image and subtracted image. (2) The evaluation of patient's image: We quantitatively analyzed patients who were examined by LEAP collimators then was classified as a normal group and who were examined by LEHR collimators then was classified as a normal group from 2008. 05 to 2009. 01. We evaluate the results from phantom by substituting factors. We used one-day protocol and injected $^{99m}Tc$-ECD 925 MBq at both basal image acquisition and diamox image acquisition. Results: (1) The evaluation of phantom: After measuring counts from each detector, at basal image 41~46 kcount, stress image 79~90 kcount, subtraction image 40~47 kcount were detected. LEAP was about 102~113 kcount at basal image, 188~210 kcount at stress image and 94~103 at subtraction image kcount were detected. The SNR of LEHR subtraction image was decreased than LEHR basal image about 37%, the SNR of LEAP subtraction image was decreased than LEAP basal image about 17%. The ratio of gray matter versus white matter is 2.2:1 at LEHR basal image and 1.9:1 at subtraction, and at LEAP basal image was 2.4:1 and subtraction image was 2:1. (2) The evaluation of patient's image: the counts acquired by LEHR collimators are about 40~60 kcounts at basal image, and 80~100 kcount at stress image. It was proper to set FWHM as 7 mm at basal and stress image and 11mm at subtraction image. LEAP was about 80~100 kcount at basal image and 180~200 kcount at stress image. LEAP images could reduce blurring by setting FWHM as 5 mm at basal and stress images and 7 mm at subtraction image. At basal and stress image, LEHR image was superior than LEAP image. But in case of subtraction image like a phantom experiment, it showed rough image because SNR of LEHR image was decreased. On the other hand, in case of subtraction LEAP image was better than LEHR image in SNR and sensitivity. In all LEHR and LEAP collimator images, proper subset and iteration frequency was 8 times. Conclusions: We could archive more clear and high SNR subtraction image by using proper filter with LEAP collimator. In case of applying one day protocol and reconstructing by Flash 3D, we could consider application of LEAP collimator to acquire better subtraction image.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2014.10a
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• pp.231-232
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• 2014

We develop a remote control system of a walking robot using a Leap motion sensor. Hand gestures and the position of fingers are provided from the Leap motion sensor. We use Processing and the LeapMotionP5 library for the development software.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.7
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• pp.1052-1061
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• 2019

Objective: This study was conducted to identify duck liver-expressed antimicrobial peptide 2 (LEAP-2) and demonstrate its antimicrobial activity against various pathogens. Methods: Tissue samples were collected from 6 to 8-week-old Pekin ducks (Anas platyrhynchos domesticus), total RNA was extracted, and cDNA was synthesized. To confirm the duck LEAP-2 transcript expression levels, quantitative real-time polymerase chain reaction was conducted. Two kinds of peptides (a linear peptide and a disulfide-type peptide) were synthesized to compare the antimicrobial activity. Then, antimicrobial activity assay and fluorescence microscopic analysis were conducted to demonstrate duck LEAP-2 bactericidal activity. Results: The duck LEAP-2 peptide sequence showed high identity with those of other avian species (>85%), as well as more than 55% of identity with mammalian sequences. LEAP-2 mRNA was highly expressed in the liver with duodenum next, and then followed by lung, spleen, bursa and jejunum and was the lowest in the muscle. Both of LEAP-2 peptides efficiently killed bacteria, although the disulfide-type LEAP-2 showed more powerful bactericidal activity. Also, gram-positive bacteria was more susceptible to duck LEAP-2 than gram-negative bacteria. Using microscopy, we confirmed that LEAP-2 peptides could kill bacteria by disrupting the bacterial cell envelope. Conclusion: Duck LEAP-2 showed its antimicrobial activity against both gram-positive and gram-negative bacteria. Disulfide bonds were important for the powerful killing effect by disrupting the bacterial cell envelope. Therefore, duck LEAP-2 can be used for effective antibiotics alternatives.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.11 no.4
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• pp.197-200
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• 1999

An extension of the modified leap-frog scheme is made to solve the nonlinear shallow-water equations. In the extended model. the physical dispersion of the Boussinesq equations is replaced by the numerical dispersion resulted from the leap-frog finite difference scheme. The model is used to simulate propagations of a solitary wave over a constant water depth and a linearly varying water depth. Obtained numerical results are compared with available analytical and other numerical solutions. A reasonable agreement is observed.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.24 no.1
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• pp.51-58
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• 2014

A LFSR is commonly used for various stream cryptography applications to generate random numbers. A Leap-ahead LFSR was presented to generate a multi-bits random number per cycle. It only requires a single LFSR and it has an advantages in hardware complexity. However, it suffers from the significant reduction of maximum period of the generated random numbers. This paper presents the new segmented Leap-ahead LFSR to solve this problem. It consists of two segmented LFSRs. We prove the efficiency of the proposed segmented architecture using the precise mathematical analysis. We also demonstrate the proposed comparison results with other counterparts using Xinilx Vertex5 FPGA. The proposed architecture can increase 2.5 times of the maximum period of generated random numbers compared to the typical Leap-ahead architecture.

• Proceedings of the Korean Society of Computer Information Conference
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• 2015.01a
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• pp.55-58
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• 2015

무선 센서 네트워크는 개방된 환경에 단거리 무선 통신으로 정보를 수집하는 센서 노드와 이를 수집하는 베이스 스테이션으로 운영된다. 이러한 센서 네트워크의 특징으로 인해 공격자를 통해 쉽게 훼손될 수 있으며 대표적인 공격방법으로 싱크홀 공격이 있다. LEAP은 싱크홀 공격에 대응하기 위해 네 종류의 키를 사용하여 노드 간 인증을 하도록 제안되었다. 이 기법은 보안성을 유지하기 위해 주기적으로 베이스 스테이션까지의 경로를 갱신한다. 본 논문에서는, 내부 싱크홀 공격을 LEAP과 같은 키의 인증을 통하여 탐지하는 기법을 제안한다. 제안 기법은 이전 노드, 다음 노드와의 키 인증을 통해 공격을 탐지한다. 공격이 탐지되면 해당 노드를 네트워크에서 제외하고 경로를 갱신하며 갱신된 경로를 통해 새로운 키를 배포한다. 그러므로 제안 기법은 이전 노드, 다음 노드와의 키 인증을 통해 싱크홀 공격을 탐지함으로써 전체 네트워크 보안성 향상을 목적으로 한다.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2016.10a
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• pp.726-728
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• 2016

In this paper, a realtime MIDI controller is designed using leap motion. The controller makes virtual MIDI port on Windows that it is connected to the port and protocol for communication with DAW or VST. It is converted MIDI data when it is detected a real time the position and shape of hand. This converted data transfer MIDI port by MIDI CC(Control Change). Therefore this controller has the purpose to support flexible MIDI input function than existed MIDI controller.

• Journal of the Korean Society of Industry Convergence
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• v.26 no.3
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• pp.463-470
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• 2023

In this study, an electronic parts assembly training system using Leap Motion was developed in consideration of the processes actually operated in the assembly process of electronic products. Based on Leap Motion and Oculus VR equipment, the system was developed to transfer user's hand movement data in real time and convert it into hand movement in virtual space so that electronic parts assembly simulation can be performed step by step. Through this, it was confirmed that the user can obtain an experience similar to the actual electronic parts assembly work, prevent errors that may occur during the assembly process, and improve proficiency. It is expected that this thesis will provide directions for the quality improvement and development of various education and training programs for virtual reality-based manufacturing processes.

• Korean Journal of Computational Design and Engineering
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• v.21 no.2
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• pp.159-169
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• 2016

In order to realize the virtual prototyping (VP) of digital products, it is important to provide the people involved in product development with the appropriate visualization and interaction of the products, and the vivid simulation of user interface (UI) behaviors in an interactive 3D virtual environment. In this paper, we propose an approach to web-based 3D virtual experience using Unity and Leap Motion. We adopt Unity as an implementation platform which easily and rapidly implements the visualization of the products and the design and simulation of their UI behaviors, and allows remote users to get an easy access to the virtual environment. Additionally, we combine Leap Motion with Unity to embody natural and immersive interaction using the user's hand gesture. Based on the proposed approach, we have developed a testbed system for web-based 3D virtual experience and applied it for the design evaluation of various digital products. Button selection test was done to investigate the quality of the interaction using Leap Motion, and a preliminary user study was also performed to show the usefulness of the proposed approach.