• Development and Reproduction
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• v.1 no.1
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• pp.9-24
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• 1997

To investigate the process of spermiogenesis of the Korean eastern Daubenton's bat, Myotis daubentonii ussuriensis, the testis obtained from mature male bats was studied by transmission electron microscope and were based on the variety and diagnostic characters of cell organells. The results obtained from the present study are as follows. According to the differentiation of the cell organells, the spermiogenesis of the Korean eastern Daubenton's bat, M. d. ussuriensis, was divided into Golg, cap, acrosome, maturation and spermiation phases. Besides, these Golgi, cap, acrosome, and maturation phase were subdivided into the steps of early and late phases repectively and matruation phase was subdivided into step of early, mid and late phases. Therfore, the spermiogenesisof M. d. ussuriensis has been divided into a total of 11 phases. The chromatin granules began to condense at the early cap phase, regularized at the acrosome phase, and a perfect nucleus of sperm was formed at the maturation phase. The chromatoid body was occurred in the upper cytoplasm of nucleus at the early Golgi phase, and it was accurred the posterior cytoplasm of the nucleus at the early maturatio phase. The formation of sperm tail began to be develop in the early golgi phase, and completed at the spermiation phase. The fiber structure of middle piece was consisted of nine outer doublets and two central singlet microtubules and Nos. 1, 5, 6 and 9 in the outer dense were larger than the others(2, 3, 4, 7, 8).

• Fibers and Polymers
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• v.4 no.4
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• pp.188-194
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• 2003

Phase behavior and spinodal phase separation kinetics in binary blends of a random copolymer of vinylidene fluoride and trifluoroethylene (75/25) [P(VDF/TrFE)] and poly(l,4-butylene adipate) (PBA) have been investigated by means of optical microscopic observation and time-resolved light scattering. The blends exhibited a typical lower critical solution temperature (LCST)∼${34}^{\circ}C$ above the melting temperature of the P(VDF/TrFE) crystals over the entire blend composition range. P(VDF/TrFE) and PBA were totally miscible in the temperature gap between the melting point of P(VDF/TrFE) and the LCST. Temperature jump experiments of the 3/7 P(VDF/TrFE)/PBA blend were carried out on a light-scattering apparatus from a single-phase melt state (${180}^{\circ}C$) to a two-phase region (205∼${215}^{\circ}C$). Since the late stage of spinodal decomposition (SD) is prevalent in the 3/7 blend, SD was analyzed using a power law scheme. Self-similarity was preserved well in the late stage of SD in the 3/7 blend.

• Journal of Yeungnam Medical Science
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• v.13 no.2
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• pp.302-307
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• 1996

Hepatic masses show different enhancing patterns in IV bolus computed tomography: Hepatocellular carcinoma shows high-attenuation in the early enhancing phase and low-attenuation in the late enhancing phase, hemangioma shows peripheral dot-like high-attenuation in the early enhancing phase and central high-attenuation in the late enhancing phase, and metastatic cancer and cholangiocelluar carcinoma show peripheral high-attenuation rim in the early enhancing phase and central portion gradulally high-attenuation in the late enhancing phase. but sometimes enhancing patterns of the hepatic masses are confuse. To evaluate the significance of the AFP level and HBsAg in differentiation of the hepatic masses, we retrospectively analyzed AFP level and HBsAg in 228 pathologically or radiologically confirmed hepatocellular carcinomas, and 137 pathologically nonhepatocellular cacinomas. The results were as follows : In hepatocellular carcinoma, AFP level above 20ng/ml was 77.8% and HBsAg positve was 72.6%. In nonhepatocellular carcinoma, AFP level above 20ng/ml was 3.7% and HBsAg positve was 16.1%. We concluded that AFP level and HBsAg are helpful to distinguish hepatocellular carcinoma from nonhepatocellular carcinoma, when IV bolus computed tomogram finding is uncertain.

• Journal of Microbiology and Biotechnology
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• v.1 no.4
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• pp.274-280
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• 1991

Effects of IPTG induction on cell growth and production of ${\beta}$-galactosidase-preS2 fusion protein (${\beta}$gal-preS2) were studied in a defined medium using a recombinant Escherichia coli JM109/pCMHB30. IPTG was added (0.2 mM) to induce the cloned-gene expression in the early-, mid-, and late-log growth phases. The most serious decreases in growth rate and plasmid stability were observed for the induction in the early-log growth phase. The expression level of ${\beta}$gal-preS2 attained by the induction in the mid-log phase was about 0.51 mg fusion protein/mg total cellular protein, which was 2- and 5-fold improvement over the levels obtained with the inductions in the early- and late-log phases. Formation of acidic byproducts including acetate and pyruvate showed different profiles during the fermentation period for each cases of induction; pyruvate was the major byproduct for the induction in the early-log phase while acetate production became more significant for the cases of inductions in the mid- and late-log phases.

• Korean Journal of Ecology and Environment
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• v.36 no.3 s.104
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• pp.269-276
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• 2003

The study was performed to understand the relationships of cyanobacterial bloom and environmental factors in Lake Daechung. The samples were collected weekly from June to October in 2001. The cyanobacterial bloom was divided into the three phases of the early phase, the middle phase and the late phase by phytoplankton standing crops. For the early phase, the correlation coefficients between chl- a and TP, and chl- a and $PO_4$-P were 0.986 and 0.894 respectively. Therefore, phosphorus was a main environmental factor in the development of cyanobacterial bloom. Zeu/Zm ratio and chl-a showed negative correlation of r = -0.995. At the late phase, $PO_4$-P showed the highest relationship (r = 0.958), and TP and temperature showed relatively high relationships (r = 0.857 and r = 0.813). At the late phase, $NH_3$-N showed highly positive relationship (r = 0.921). It was confirmed that $PO_4$-P was the most important contribution factor for the bloom through the regression analysis on the environmental factors. As the result, the decrease of Zeu/Zm ratio and the increase of P concentration influenced cyanobacterial bloom developed rapidly in the early phase. Also the cyanobacterial bloom was decreased in proportion to decreasing of $NH_3$-N concentration in the late phase. It was expected that observation of $NH_3$-N may be a very useful factor on monitoring of the decreasing situation of the bloom.

• Journal of Acupuncture Research
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• v.17 no.2
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• pp.139-152
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• 2000

This study was designed to evaluate the analgesic effect of bee venom (BV) Acupuncture into different treatment points, Chok-samni (ST36) and blank loci of the gluteal muscle and back. We investigated the changes in formalin-induced pain behavior according to the pretreatment with different concentrations of BV, thirty minutes before the formalin injection. The results were summarized as follows: 1. The formalin-induced pain behavior was suppressed by pretreatment with BV into Chok-samni (ST36) in a dose dependent manner. During the early phase, 0.08mg/kg of BV showed a statistically significant suppression in the formalin-induced pain behavior. Moreover, 0.008mg/kg, 0.016mg/kg, and 0.08mg/kg of BV, except 0.0016mh/kg of BV, had significant suppresive effects on the formalin-induced pain behavior during the late phase. Therefore, these data indicated that the suppressive effect of BV acupuncture on the formalin-induced pain behavior was stronger in the late phase rather than the early phase 2. In order to investigate the analgesic effect of BV acupuncture into different treatment points, the experimental animals were divided into three groups: Chok-samni (ST36) group, gluteal group and back group. In the Chok-samni (ST36) group, the formalin-induced pain behavior during all the phases was significantly reduced as compared with that of the back group. However, as compared with that of the gluteal group, the formalin-induced pain behavior in the Chok-samni (ST36) group was decreased only in the late phase, not in the early phase. The formalin-induced pain behavior in the gluteal group was significantly reduced as compared with that of the back group in the late phase, not in the early phase. We suggested that the analgesic effect of BV acupuncture into Chok-samni (ST36) was most effective among Chok-samni (ST36), gluteal, and the back groups in formalin-induced pain behavior.

• Journal of Animal Science and Technology
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• v.52 no.1
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• pp.29-36
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• 2010

This experiment was conducted to evaluate the influence of phase feeding methods on growth performance, carcass characteristics, pork quality, and economical efficiency in growing-finishing pigs. A total of 120 crossbred pigs ([Yorkshire ${\times}$ Landrace] ${\times}$ Duroc), average initial body weight 25.23 ${\pm}$ 2.66 kg, were allotted to 5 treatments by body weight and sex in a randomized complete block (RCB) design. Each treatment had 6 replicates with 4 pigs per pen. The treatments were 1) A (fed late weaner feed during whole phase), 2) B (fed late weaner feed in growing phase and grower feed in finishing phase), 3) C (fed grower feed during whole phase), 4) D (fed grower feed in growing phase and early finisher feed in early and late finishing phase), 5) E (fed grower feed in growing phase, early finisher feed in early finishing phase and late finisher feed in late finishing phase). Commercial diets bought from private feed company were used for this experiment. During the early growing phase (6 wks), treatments A and B showed higher growth performance than that of other treatments (P<0.05). However, growth performance tended to be similar among treatments at 10 wks after feeding and there was no difference in time of market weight (13 wks). Moreover, blood urea nitrogen (BUN) concentration was lowered in treatment E than other treatments during whole experimental period (P<0.01). Carcass characteristics and pork quality were measured when body weight of pigs reached at 110.33 ${\pm}$ 2.1 kg. Days to market weight (110 kg), quality grade, pork color, and pork pH did not show any significant difference among treatments. These results suggested that phase feeding based upon growth stages of pigs showed no detrimental effects on growth performance and the pork quality. Moreover, feed cost was saved approximately 37% by phase feeding method without delaying of days to market weight compared to general conventional feeding method (B) of swine farm in Korea.

• Reproductive and Developmental Biology
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• v.37 no.3
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• pp.149-154
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• 2013

The aim of this study was to evaluate the changes of protein patterns in granulosa cells and corpus luteum in ovaries during the estrus cycle in cows. The estrus cycle was devided into five steps of follicular, ovulatory, early-luteal, mid-luteal and late-luteal phases. In results, 61 spots of total 85 spots were repeated on follicular phase and 51 spots of total 114 spots were repeated on ovulatory phase. The 40 spots of total 129 spots were repeated on early-luteal phase and 49 spots of total 104 spots were repeated on mid-luteal phase. Also 41 spots of total 60 spots were repeated on late-luteal phase. On the other hands, the 16 spots were indicated difference in follicular phase and ovulation phase had a difference 10 spots. It was showed difference No. 103 spot in ovulation phase, No. 135 spot in early-luteal phase and No. 175 and 176 spots in mid-luteal phase. Also, the 11 spots were expressed specifically in mid-luteal phase and No. 178 and 179 spots were difference of expression in late-luteal phase. We confirmed that there were 7 spots for ovulation, 4 spots for luteinization and 2 spots for luteolysis. Spot No. 89~93 in ovulation phase were transferrin, and spot No.94~98 were HSP60. Spot No. 103 was Dusty PK, spot No. 135 was OGDC-E2, and spot No. 175 and 176 were Rab GDI beta from luteinization. Spot No. 178 and 179 in luteolysis were vimentin. This results suggest that will be help to basic data about infertility.

• Clinical and Experimental Reproductive Medicine
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• v.13 no.2
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• pp.113-119
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• 1986

The present study was designed to assess the relationships between the methods to evaluate adequacy of luteal function. We measured mid-luteal serum progesterone levels by radioimmune assay, luteal phase lengths and mean post-ovulatory basal body temperature rise rates by basal body temperature charts in 40 in-phase-cycle infertile patients and 38 out-of-phase-cycle patients who underwent late-luteal endometrial biopsies at the sterility clinic of Department of Obstetrics and Gynecology, Seoul National University Hospital from Jan. 1986 to Aug. 1986. The results were summarized as follows: 1. No significant differences were found in mean age, mean duration of infertility, mean mid-luteal serum progesterone levels, and mean post-ovulatory temperature rise rate between in-phase-cycle patients and out-of-phase-cycle patients, but significant difference in mean luteal phase length between 2 groups was identified. 2. 91% of total patients sho had luteal phase lengths of less than 11 days showed out-of-phase-cycles. 3. In out-of-phase-cycle group with luteal phase lengths of less than 11 days, 50% had an endometrial lag of at least 4 days, but 10.7% had an endometrial lag of 4 or more days in group with luteal phase lengths of more than 11 days. 4. There was no significant correlation between mid-luteal serum progesterone level and endometrial lag of late luteal phase endometrial biopsy.

• Korean Journal of Applied Biomechanics
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• v.15 no.4
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• pp.67-74
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• 2005

The purpose of this study is to compare and analyze the muscle activations between the professional golfers without low back pain symptom and the professional golfers with low back pain symptom, and so identify the stress related to golf swings, and provide the basic data to minimize the low back pain and the injury risk. Using surface electrode electromyography, we evaluated muscle activity in 6 male professional golfers during the golf drive swing. Surface electrodes were used to record the level of muscle activity in the Abdominal Oblique, Elector Spinae, Rectus Abdominis, Gluteus Maximus muscles during the golfer's swing. These signals were compared with %RVC(Reference voluntary contraction) which was normalized by IEMG(Integrated EMG). The golf swing was divided into five phases: take away, forward swing, acceleration, early follow through, late follow through. we observed patterns of trunk muscle activity throughout five phases of the golf swing. The results can be summarized as follows: RES(Right Elector Spinae) had statistically significant difference in take away phase, LGM(Left Gluteus Maximus), LRA(Left Rectus Abdominis), LOA(Left Oblique Abdominal) had statistically significant difference in forward swing phase, RES(Right Elector Spinae), RGM(Right Gluteus Maximus), ROA(Right Oblique Abdominal) had statistically significant difference in acceleration phase, RES(Right Elector Spinae), RGM(Right Gluteus Maximus) had statistically significant difference in early follow-through phase, LES(Left Elector Spinae), RGM(Right Gluteus Maximus) had statistically significant difference in late follow through phase.