Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Change of Protein Patterns in Granulosa Cells and Corpus Luteum during the Ovarian Cycle in Cows

소의 난소주기 동안 과립막세포와 황체조직에서 단백질 패턴의 변화

  • Song, Eun-Ji (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Yong-Seung (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Sang-Hee (College of Animal Life Sciences, Kangwon National University) ;
  • Yoo, Han-Jun (College of Animal Life Sciences, Kangwon National University) ;
  • Park, Joung-Jun (Myung-poom Hanwoo Consulting) ;
  • Cheong, Hee-Tae (College of Veterinary Medicine, Kangwon National University) ;
  • Yang, Boo-Keun (College of Animal Life Sciences, Kangwon National University) ;
  • Park, Choon-Keun (College of Animal Life Sciences, Kangwon National University)
  • 송은지 (강원대학교 동물생명과학대학) ;
  • 이용승 (강원대학교 동물생명과학대학) ;
  • 이상희 (강원대학교 동물생명과학대학) ;
  • 유한준 (강원대학교 동물생명과학대학) ;
  • 박정준 (명품한우 컨설팅) ;
  • 정희태 (강원대학교 수의과대학) ;
  • 양부근 (강원대학교 동물생명과학대학) ;
  • 박춘근 (강원대학교 동물생명과학대학)
  • Received : 2013.09.12
  • Accepted : 2013.09.23
  • Published : 2013.09.30
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Abstract

The aim of this study was to evaluate the changes of protein patterns in granulosa cells and corpus luteum in ovaries during the estrus cycle in cows. The estrus cycle was devided into five steps of follicular, ovulatory, early-luteal, mid-luteal and late-luteal phases. In results, 61 spots of total 85 spots were repeated on follicular phase and 51 spots of total 114 spots were repeated on ovulatory phase. The 40 spots of total 129 spots were repeated on early-luteal phase and 49 spots of total 104 spots were repeated on mid-luteal phase. Also 41 spots of total 60 spots were repeated on late-luteal phase. On the other hands, the 16 spots were indicated difference in follicular phase and ovulation phase had a difference 10 spots. It was showed difference No. 103 spot in ovulation phase, No. 135 spot in early-luteal phase and No. 175 and 176 spots in mid-luteal phase. Also, the 11 spots were expressed specifically in mid-luteal phase and No. 178 and 179 spots were difference of expression in late-luteal phase. We confirmed that there were 7 spots for ovulation, 4 spots for luteinization and 2 spots for luteolysis. Spot No. 89~93 in ovulation phase were transferrin, and spot No.94~98 were HSP60. Spot No. 103 was Dusty PK, spot No. 135 was OGDC-E2, and spot No. 175 and 176 were Rab GDI beta from luteinization. Spot No. 178 and 179 in luteolysis were vimentin. This results suggest that will be help to basic data about infertility.

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References

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