• Korean journal of applied entomology
• /
• v.25 no.2 s.67
• /
• pp.93-98
• /
• 1986

The densities of plant-and leaf hoppers were examined by direct counting the rice plant of two varieties; Milyang 23 and Chucheongbyeo, in Chinju. The densities of the small brown planthopper(SBPH), Laodelphax striatellus, and Green rice leafhopper(GLH), Nephotettix cincticeps were higher on Chuchungbyeo than on Milyang 23 throughout the season, whereas those of white backed planthopper(WBPH), Sogatella furcifera, and Brown planthopper(BPH), Nilaparvata lugens were high on Milyang 23. Seasonal densities of planthoppers were varied with rice plant growth; on Chucheong field, GLH was the predominant species from June to August and BPH was in September, but in Milyang 23 field, WBPH was more abundent from July to August and BPH was increased from late August and was the highest on September.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2020.12a
• /
• pp.63-63
• /
• 2020

Rice stripe virus (RSV) disease is one of the major constraints in rice production, transmitted by the small brown planthopper (SBPH; Laodelphax striatellus). Upon RSV infection, plants develop typical symptoms, which include chlorosis and weakness of newly emerged leaves, white and yellow spots, stripe on leaves, and necrotic and wilting leaves, resulting in plant growth inhibition, oxidative damage that may culminate in programmed cell death (PCD) and plant death in severe epidemics. Although RSV-resistant quantitative trait loci (QTLs), Stv-a, Stv-b, and Stv-bi, were mapped using various resistant varieties, one RSV-resistant gene, OsSOT1, has been identified so far. In this study, we used the rice cultivar Zenith, known to carry Stv-b, to investigate novel RSV-genes through fine mapping. Therefore, we crossed Zenith (Donor parent, RSV resistant) with Ilpum (Recurrent parent, RSV susceptible) to fine-map using a BC₂F₂ population of 2100 plants. Chromosome segment introgression lines that were heterozygous at a different region were selected, two types of heterozygous lines showed an heterozygous genotype between Sid2 and Sid75 to Indel9 and RM6680. Interestingly, we identified qSTV11^Z region harboring Stv-b, covering about 171-kb region between the InDel markers Sid75 and Indel8. The localization of qSTV11^Z provides useful information that could be used for marker-assisted selection and determination of genetic resources in rice breeding.

• Journal of Ecology and Environment
• /
• v.47 no.4
• /
• pp.250-260
• /
• 2023

Background: Crabgrass (Digitaria ciliaris, Poaceae) is a globally distributed weed, including in Afro-Eurasia, America, and Australia. As a highly gregarious plant, crabgrass is an important habitat for a diverse array of insects, and a potential habitat for agricultural pests. To compare the insect communities associated with the crabgrass community, insects were sampled using sweep sampling (100 sweeps per sample) at five sites, including Daejeon (Daejeon and Gap rivers), Anseong, Namhae, and Inje, with a focus on the Daejeon River. Results: A total of 5,888 individual insects belonging to eight orders, 42 families, and 115 species were collected from the five sites. Both the number of species and individuals of Hemiptera were the highest at all of the sites. In the present study, 73% of the insect population fed on D. ciliaris as a host plant. The dominant species in the D. ciliaris community was Laodelphax striatellus (Delphacidae), being ubiquitous at all the sites which showed a high abundance of rice pests in the communities and the suitability of D. ciliaris as an alternative host plant for them. The Shannon-Wiener diversity index was highest in Inje on 17 September (2.88), and the Chao1-bc diversity index was highest in the Gap River on 5 September (80). The sampling efficiency of 100 sweep samples (sample coverage) was calculated to be as high as 90%. The results of the samples taken from September to November in the Daejeon River showed that the number of species and individuals decreased gradually over time, and the number of dominant species decreased sharply between September and October. Similarity analysis indicated that sampling dates that were closer together yielded sampled assemblages with higher faunal similarity. In addition, in each sampling, the difference in the minimum temperature during the two-week period prior to sampling and faunal similarities were negatively correlated. Conclusions: This study provides foundational data that could enhance our understanding of insect diversity in D. ciliaris. The data can facilitate ecological conservation and management of Korean grasslands generally, as well as identification of potential pests that may disperse from D. ciliaris communities to nearby farmland.

• Korean journal of applied entomology
• /
• v.57 no.4
• /
• pp.393-399
• /
• 2018

A loop-mediated isothermal amplification (LAMP) primer set (WBPH-65) was designed for the species-specific detection of white-backed planthopper (WBPH) Sogatella furcifera based on the full-length sequence of the internal transcribed spacer 2 (ITS2) (KC417469.1). The WBPH-65 primer set consists of six primers (total 165 bp), F3 (18 bp), B3 (18 bp), FIP (43 bp), BIP (40 bp), LF (21 bp), and LB (25 bp). After the LAMP reaction of three rice planthoppers, S. furcifera, Nilaparvata lugens, and Laodelphax striatellus, with the WBPH-65 primer set for 60 min at $65^{\circ}C$, the LAMP products were observed in the genomic DNA of S. furcifera only. According to the DNA amount of S. furcifera and incubation duration at $65^{\circ}C$, the difference of fluorescence relative to the negative control (0 ng) was clearly observed in a 40-min incubation with 10 and 100 ng or in case of 60-min incubation with 0.01, 0.1, 1, 10, and 100 ng. There was little difference in fluorescence between the negative control and all the other DNAs tested in 20- and 30-min incubations. On the other hand, the WBPH-65 primer set without LF and LB primers showed little amplification in the genomic DNAs of the three rice planthoppers, S. furcifera, N. lugens, and L. striatellus in a 60-min incubation. These results suggest that all six primers (F3, B3, FIP, BIP, LF, and BF) are necessary for the WBPH-65 primer set to detect S. furcifera within a 60-min incubation, and is able to discriminate S. furcifera from at least N. lugens and L. striatellus.

• Korean journal of applied entomology
• /
• v.15 no.1 s.26
• /
• pp.1-6
• /
• 1976

his study was conducted to investigate selective toxicity of several organophosphorous and carbamic insecticides against the small brown planthopper (Laodelphax striatellus), the white-backed planthopper (Sogatella furcifera), the brown planthopper (Nilaparvata lugensEugens), and the green rice leafhopper (Nephotetix cincticeps). The test insecticides were the organophosphoric insecticides, Diazinon$(34\%\; Ec.,\; 3\%\; G.),$ $MEP(Sumithion 50\%\; Ec.)$, and PAP(Elsan\; 47.5\%\; Ec.)$ and the carbamic insecticides. MPMC $(Meobal\; 50\%\;WP.)$, $BPMC(Bassa\; 50\%\; Ec.)$, and carbofuran $(Curaterr; 3\%\; G.)$. Toxicitiy for emulsion concentrates and wettable powders were evaluated by dipping method of rice seedlings in the laboratory and that for granulated insecticides evaluated by submerged application with potted plants in the greenhouse. In the test of contact toxicity by seedling dips, Diazinon MEP and PAP showed significantly low toxicity to the brown planthopper and MEP to the green rice leafhopper. In the test of submerged application, Carbofuran showed almost equal toxicity to the four species of the hoppers, but Diazinon was greatly less toxic to the brown planthopper. In general. the organophosphorous insecticides tested seemed to be selective in toxicity to the species of the hoppers, while the carbamic insecticides being less selective. The carbamic insecticides such as MPMC, BPMC and Carbofuran were almost equal in toxicity to the four species of the hoppers tested.

• Korean journal of applied entomology
• /
• v.15 no.4 s.29
• /
• pp.179-184
• /
• 1976

Laboratory experiments were conducted to clarity the early-spline host·selectivity by the small brown planthopper(SBPH), Laodelphax striatellus Fallen, and green rice Leafhopper(GLH), Nephotettix cincticeps Uhle., on the bailey (the variety Suweon #18) and water foxtail (Alopeculus aequadis Sosbol). The host selectivity was evaluated on the bases of feeding and ovipositional perferences of the insects on the plants and their biological effects on the plants. The nymphs of SBPH much more preferred barley for feeding than water forxtail, while the nymphs of GLH relatively prefered water foxtail. There was no significant difference in ovipositional preference by SBPH among the test plants, and ovipositional preference by GLH Ivas significantly lower on barley and water foxtail than on rice. Nymphal growth an4 adult emergence of SBPH were significantly faster and higher on barley than on water foxtail. No adult emergence of GLH was observed on barley, and adult emergence was still quite lower even on water foxtail. The adult of SBPH fed on barley showed longer longevity aad higher fecundity than that of SBPH from water foxtail and rice. The adult GLH fed ell water foxtail shorted relatively shorter longevity and to)ver fecundity than that of GLH on rice. In conculsion, barley seems to be more adequate for spring host of SBPH than water foxtail, but barley may not be quite adequate for spring host of GLH. At presont moment, GLH seems rather to primarily select the water foxtail than barley as a spring host, even if the water foxtail is not so adequate for development of GLH.

• Korean Journal Plant Pathology
• /
• v.1 no.2
• /
• pp.109-114
• /
• 1985

The experiments were conducted to clarify the influence of transplanting date on the occurrence of rice virus in field condition of 1984. The rate of RBSDV (rice black-slreaked dwarf virus) viruliferous vector, smaller brown planthopper (Laodelphax striatellus Fallen), was shown to be $13.6\%$ at the 2nd adult and that of rice stripe virus (RSV) viruliferous was $6.7\%$ at the 2nd adult. The vector in the field was begun to come from May 29, the maximum densities were 19.6 insects per hill on June 13 in cultivar Chucheongbyeo, 19.3 in Nagdongbyeo, 7.4 in Cheongcheongbyeo and 4.9 in Samgangoyes. The number of vectors per hill was inclined to increase by early transplanting. Although the infection of rice virus in nursery bed was not recognized until May 30 transplanting, the nursery infection could be seen from June 10 transplanting. The highest rate of nursery infection with RSV was $4.1\%$ at June 10 transplanting plot, and that of RBSDV was $14.2\%$at June 20 trans planting plot. The infection of rice virus in paddy field was the highest at May 20 transplanting plot, the lowest at July 10 plot. The earlier transplanting, the more severe occurrence of rice viruses. Occurrence of infected plants with RBSDV was shown to increase more rapidly at May 20 and May 30 planting plot than May 10 plots. However, the occurrence of infected plant with RSV was more rapid at May 10 transplanting plot than May 20 and 30.

• Research in Plant Disease
• /
• v.18 no.3
• /
• pp.245-249
• /
• 2012

The small brown planthopper (Laodelphax striatellus) is one of the most important rice pests in Republic of Korea because it damages rice plants not only by sap-sucking but also by transmitting Rice stripe virus (RSV). Outbreaks of RSV are closely related to outbreaks of the small brown planthopper (SBPH). Therefore, it is very important to control SBPH for the management of RSV. Mass-migrating SBPH collected by aerial net traps in June 2011 at Taeanup, Geunheungmyon and Gonammyon in Taeangun were examined for virus carrier status and effects of the pesticide, 'Myungtaja', on the control of RSV. Among 1,217 SBPH trapped, about 7.7% were detected as RSV positive and 4.4% were positive for Rice black streak dwarf virus (RBSDV) by RT-PCR. After the mass migration, pesticide 'Myungtaja' was sprayed once or twice on rice fields and compared to untreated fields. The incidence of RSV was not affected by the frequency of spraying 'Myungtaja' but was influenced by the time of pesticide treatment. Myungtaja' treatment within 5-7 days after mass migration resulted in the most efficient RSV control, resulting in RSV incidence decreased by 87.6% compared to the control. Therefore, we conclude that pesticide spraying for RSV control was most effective when it was done within 5-7 days after mass migration.

• Korean journal of applied entomology
• /
• v.16 no.2 s.31
• /
• pp.79-86
• /
• 1977

Four experiments were carried out under farmer's field conditions to determine economic threshold levels of major rice pests aad attempt a reduction in the number of insecticide applications. In the experiments were included check treatments, insecticide schedules representing the official recommendations to farmers, and several corrective treatments. A careful record was kept of insect pest densities and disease incidence. i) In the north at Suweon and Icheon where Chilo suppresalis. (Walk.), the striped rice borer, was active in the first generation, no significant yield differences were obtained between plots receiving several insecticide applications and those left totally unprotected. The mean yields were high (5.2-7.6t/ha). ii) First generation borer activity rising to $8.6\%$ injured tillers was below the economic threshold since no yield reduction was recorded in either japonica varieties or the high-yielding Tongil variety. iii) Evidence was obtained thst berer damage was made good by replacement of infested tillers (compensatory growth), C. suppressalis populations were always low in the second generation. iv) The experimental results obtained at Suweon and Icheon do not justify the present official recommendations of 6-7 pesticide applications. v) further south at Iri a substantial yield reduction occurred due to an early outbreak of stripe disease transmitted by Laodelphax striatellus (Fallen), the small brown planthopper; a mean of 1-2 individuals/hill was recorded immediately after transplanting. C. suppressalis probably contributed to this yield reduction. vi) Several applications against the vector failed to prevent the rapid spread of stripe to the susceptible variety in the Iri experiment: in surrounding fields the resistant Tonsil varivety was ralatively unaffected. vii) Pests of lesser importance were Nephotettix cinctieps (Uhler), Nilaparvata lugens (Stil), Sogatella furcifera (Horv..), and leaf miners.

• Korean Journal of Environment and Ecology
• /
• v.30 no.4
• /
• pp.712-723
• /
• 2016

This paper is to suggest the methods about the environmental risk assessment (ERA) based on non-target insect species for LM rice crop produced by biotechnology. We used some data by the picture dictionary of agricultural pests in Korea, some books for ERA or rice pest ecology, articles about rice ecology, and internet sites and many specialists gave us good advice for this ERA system. We found about 140 insect species using rice crop as their habitat and we discriminated herbivores into Sap feeder, Grain feeder, Defoliator, and Pollen feeder according to their food functional group. We also clarified the potential damage possibility of insect species from LM rice crop. Then, we ranked 10 non-target insect species (Baliothrips biformis, Laodelphax striatellus, Nilaparvata lugens, Mythimna separata, Cnaphalocrocis medinalis, Cletus schmidti Kiritshenko, Scotinophara lurida, Nilaparvata bakeri, Oxya chinensis, Agromyza oryzae) with giving some scores using Geographic distribution, Habitat specialization, Prevalence on crop, Abundance. We showed some environmental risk hypotheses about herbivore, predator, and parasitoid, and infered the impacts of non-target species on LM rice crop. Conclusively, we want to serve this methodology to build the biosafety system from biotechnology crops, and the methodology is to keep public health, sustain the biodiversity, and conserve the natural ecosystems.