• Environmental Mutagens and Carcinogens
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• v.24 no.3
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• pp.113-120
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• 2004

Chromium compounds are known human and animal carcinogens. In this study, the effects of sodium chromate on apoptosis and cell cycle were investigated in order to unveil the elements of early cellular responses to the metal. Using Chinese hamster ovary cells(CHO-K1-BH4), we found taht chromium (VI) treatment induced apoptosis in these cells, as signified by nuclear fragmentation, DNA laddering on agarose gel electrophoresis, and an increased proportionof cells with hypodiploid DNA. Preceding these changes, chromium (VI) treatment increased caspase 3 pritease activity and also increased expression of p53 protein, while the level of bcl2 protein was not changed. Coincubation with caspase inhibitor, Z-DEVD-FMK, inhibited chromium-induced apoptosis. In the flow cytometric analysis using propidium iodide fluorescence, an increase of cell population in G2/M phase was shown in cells exposed to at least 160 $\mu\textrm{m}$ of sodium chromate for 72h, form 9.8% for 0$\mu\textrm{m}$ chromium (VI) to 26.4% for 320$\mu\textrm{m}$ chromium(VI). Taken together, these findings suggest that chromium(VI)-induced apoptosis is accompanied by G2/M cell cycle arrest, and that p53-mediated pathway may be involved in positive regulation of G2/M arrest and a concurred apoptosis in CHO cells.

• Journal of Information Technology Services
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• v.19 no.1
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• pp.113-127
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• 2020

Current web accessibility checks and automatic assessments have been pointed out that the assessment items and scores are evaluated from the developer's point of view rather than from the user's one. In addition, although most of the grades of an automatic assessment on the public web sites are excellent because they are built in accordance with the web accessibility development guidelines, not a few web sites shows relatively low grades in evaluating their usability test made by those users. Taking into account the inadequacy of these web accessibility assessments, this study has identified the differences between the grades of usability evaluations and automatic evaluations for people with low vision and analyzed the major factors affecting web accessibility usability evaluations using Repertory Grid Techniques. Also, the Hard Laddering method of the Means-End Chain theory was adopted to visualize the relationship between Attributes-Conferences-Value and a hierarchical value system analysis based on FGI(Focused Group Interview) to people with the low vision. This study proposed the measures to improve the current web accessibility automatic assessment allocation, expert evaluation criteria, and user task assessment. In particular, it is a web accessibility user evaluation model that can consider the web accessibility quality certification criteria and user review assessment by directly analyzing the user cognitive structure and value system. This study is expected to be useful as a research to enhance the quality of web accessibility assessment.

• Journal of the Korean Society of Clothing and Textiles
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• v.18 no.5
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• pp.674-681
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• 1994

In this study, four kinds of vapor-permeable waterproofed fabrics were selected and their properties and the changes of properties were investigated after laundering, petroleum drycleaning or tetrachloroethylene drycleaning (each 1, 5, 10 times). The results were as follows: 1) In the water resistance, only Gore Tex satisfied its minimum property requirements, $1.0kg/cm^2$ It decreased after laddering and drycleaning. Hipora-1000, Hipora-2000, Aitac decreased slightly by petroleum drycleaning and Gore Tex decreased slightly by all three methods. 2) In the moisture vapor transmission, three except Hipora-2000 satisfied its minimum property requirement, $4000g/m^2{\cdot}24hr$.24hr. After laundering and drycleaning, it increased regardless of cleaning methods. 3) In the water repellency, Hipora-lOOG, Hipora-2000 were not almost decreased after laundering and drycleaning. Aitac decreased slightly by petrolem drycleaning and Gore Tex decreased slightly by laundering and petroleum drycleaning. 4) Air permeability of Aitac increased with increasing of cleaning times regardless of cleaning methods. 5) In the bond strength, only Gore Tex dissatisfied its minimum property requirements, 250g/cm. Regardless of cleaning method, bond strength decreased with increasing of cleaning times. 6) Color difference was large in case of Hipora-1000 by laundering, Hipora-2000 by laundering and tetrachloroethylene drycleaning Aitac by tetrachloroethylene drycleaning, Gore Tex by laundering. To diminish property change of vaper-permeable waterproofed fabric, petroleum drycleaning is more effective than laundering and tetrachloroethylene drycleaning.

• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.94-94
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• 2003

Endocrine disrupters (EDs) are exogenous chemicals that interfere with the production, releasing, metabolism, excretion, binding of natural hormones, and whole endocrine systems. EDs are very dangerous since they are extremely stable, not easily degraded, and accumulated in fat and tissue. 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) is known as the most toxic EDs. Therefore, this study was conducted to investigate the effects of TCDD on proliferation of human breast cancer (MCF-7) and endometrial adenocarcinoma (Hec-1B) cells. 10, 100, and 1000 nM of TCDD were treated with steroid free condition. Viable cell counting, MTT, and BrdU assay was performed to investigate cell proliferation. Apoptosis was investigated using DNA laddering. Although, DNA fragmentation as the evidence of apoptosis was not detected, all of these cell lines showed restricted proliferation at 48 hrs after 100 and 1000 nM TCDD treatments. Recently, it has been reported that the expression of transforming growth factor $\beta$s (TGF-$\beta$s) are increased in TCDD treatment and also involved in regulation of cell cycle. Therefore, these results were considered that the decreased cell prolifcration by TCDD is related to the expression of TGF-$\beta$s.

• The Korean Journal of Physiology and Pharmacology
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• v.13 no.3
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• pp.139-145
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• 2009

The purpose of this study was to evaluate the effects of the water extract of Samultang (SMT), a Chinese herb, on apoptotic cell death by $H_2O_2$-induced oxidative stress in SK-N-M C cells. A nuclear fragmentation was observed via fluorescence imaging 12 h after exposure to 30 ${\mu}M$ $H_2O_2$ and DNA laddering was detected via agarose electrophoresis gel. In addition, increases in sub-G1 phase and cleavage of the PARP protein were observed. However, treatment with SMT for 2 h prior to $H_2O_2$ exposure significantly reduced apoptotic cell death induced by incubation with 30 ${\mu}M$ $H_2O_2$ in SK-N-MC cells. Pre-incubation with water extract of SMT for 2 h prevented the $H_2O_2$-induced decrease in mitochondrial transmembrane potential. SMT also attenuated the increase in caspase-3 activity and the breakdown of PARP protein caused by $H_2O_2$-induced oxidative stress. These results suggest that the water extract of SMT provides inhibition of apoptotic cell death against oxidative injury in SK-N-MC cells.

• Archives of design research
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• v.14 no.2
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• pp.107-116
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• 2001

Nowadays, The market environment is not static, but dynamic, it therefore requires global competition for the competitive advantage of product design in global market across boundaries. So if companies do not thoroughly understand core values, needs of consumers, and do not properly meet them, they cannot get the competitive advantage in global market for consumers. Therefore, the goal of this research is to develop a new design process for global markets through developing the on-line research analysis program and data-base. For this study, the researchers established a research framework associated with values, benefits and design attributes and developed a Global Design Process program through empirical researches. This research program is a upgraded system adapted to the digital period drastically.

• Journal of Life Science
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• v.12 no.2
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• pp.182-187
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• 2002

In this study we have investigated the effect of Caesalpina sappann L. extracts on the apoptosis in NIH3T3 cells exposed to methylmethan sulfonate (MMS), an alkylating agent. MTT assay study showed that Caesalpina sappan L. extracts potentiate the MMS-induced viability. Cell morphology studies, acridine orange (AO) staining, and DNA fragmentation analysis indicated that the postincubation of Caesalpina sappan L. extracts increase the nuclear condensation of MMS-induced apoptotosis. These results suggest that Caesalpina sampan L. extracts contain components potentiating MMS-induced apoptosis of NIH3T3 cells.

• Herbal Formula Science
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• v.22 no.2
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• pp.35-43
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• 2014

Objectives : The purpose of this study was to identify antiproliferative effects of Salviae miltiorrhizae Radix(SM) extracts against cancer cell lines. Methods : We used 2 kinds of cancer cell lines such as colon cancer cells(HT-29), human oral epitheloid carcinoma cells(KB). MTT assay was performed to examine the efficacy of SM extracts on the cytostaticity of cancer cells in proportion to time and doses. Apoptosis was evaluated by DNA laddering and DAPI nuclei staining. Results : The MTT absorbances against HT-29 and KB of SM extracts were significantly decresed. DNA ladders could be identified in KB of SM extracts. The morphological change were observed and number of cells were decreased by SM extracts. Conclusions : SM extracts is considered to be effective to induce apoptosis and inhibit cancer cell proliferation.

• Design & Manufacturing
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• v.10 no.2
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• pp.6-11
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• 2016

Designed patterns of slit are introduced to the uni-directional (UD) prepreg in order to enhance the formability of the carbon/epoxy composites without wrinkling and laddering. Three different types of the slit alignment along the thickness direction are applied to analyse the deformation behavior during the compression moulding process of laminates. Degrees of the slit open and the mechanical strength are evaluated based on the level of the compaction pressure in the course of forming process. Results have shown that the mechanical strength of laminates having slits could attain at least 80% of the conventional ones without slits. However, further studies are required to identify the direct relevance of the slit alignment in laminate to the mechanical properties.

• The Journal of Korean Obstetrics and Gynecology
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• v.21 no.4
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• pp.90-103
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• 2008

목적 : 본 연구는 현호색의 메탄올 추출물(Corydalis Tuber Extract:CTE)이 인간 유방암 세포인 MCF-7 세포의 자멸사에 미치는 영향을 알아보고자 하였다. 방법 : 현호색 메탄올 추출물로 인간유방암에서 유래된 MCF-7 세포를 처리하였다. CTE의 세포자멸사 유도 효과는 MTT, FACS, TUNEL, DNA laddering and immunoblot assay를 통하여 측정하였다. 결과: 본 연구에서 현호색 메탄올 추출물은 MCF-7 세포의 활성을 감소시켰다. CTE로 처리한 MCF-7 세포는 용량에 따라 세포 자멸사 과정이 증가했다. CTE에 노출하였을 때 Bcl-2와 $Bcl-_{XL}$의 발현을 억제하고, 미토콘드리아로부터 세포질로 cytochrome-c를 방출하며, caspase와 PARP의 절단을 유발하여 세포자멸사가 증가했다. 현호색 메탄올 추출물에 존재하는 성분중에서 coptisine이 세포 활성도를 효과적으로 감소시킨 것으로 사료된다. 결론: 실험결과 현호색 메탄올 추출물이 유방암의 임상 치료에 있어 가능성 있는 치료 방법이 될 수 있을 것으로 사료된다.