• BMB Reports
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• v.50 no.4
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• pp.158-159
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• 2017

MicroRNAs (miRNAs) regulate gene expression by guiding the Argonaute (Ago)-containing RNA-induced silencing complex (RISC) to specific target mRNA molecules. It is well established that miRNAs are stabilized by Ago proteins, but the molecular features that trigger miRNA destabilization from Ago proteins remain largely unknown. To explore the molecular mechanisms of how targets affect the stability of miRNAs in human Ago (hAgo) proteins, we employed an in vitro system that consisted of a minimal hAgo2-RISC in HEK293T cell lysates. Surprisingly, we found that miRNAs are drastically destabilized by binding to seedless, non-canonical targets. We showed that miRNAs are destabilized at their 3' ends during this process, which is largely attributed to the conformational flexibility of the L1-PAZ domain. Based on these results, we propose that non-canonical targets may play an important regulatory role in controlling the stability of miRNAs, instead of being regulated by miRNAs.

• Journal of Environmental Science International
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• v.21 no.9
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• pp.1079-1085
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• 2012

Ground seawater quality was investigated, and the algal growth potential (AGP) tests were performed along the Tongyeong coast to examine the inflow of materials, which is needed for the red tide species Cochlodinium polykrikoides to grow. The study was conducted to determine the mechanism for C. polykrikoidesred tides. Water temperature, salinity, pH, and dissolved oxygen (DO) ranged from 16.05 to $20.74^{\circ}C$, 18.20 to 32.11 psu, 6.00 to 7.61, and 3.41 to 7.91 mg/L (41.4-96.1%), respectively. No seasonal variation was observed in water temperature. The salinity, pH, and DO saturations at most stations were lower than those of coastal seawater at Tongyeong. The ${NH_4}^+$-N, ${NO_{2+3}}^-$-N, ${PO_4}^{3-}$-P concentrations ranged from 0.43 to 16.00 ${\mu}M$, 1.50 to 132.38 ${\mu}M$, and 1.30 to 6.29 ${\mu}M$, respectively; the values at some stations were much higher than observed in Tongyeong coast seawater. Using seawater from station B, C. polykrikoides grew consistently, with a high growth rate, similar to the red tide in nature. This seawater appeared to contain materials needed by C. polykrikoides to grow. Therefore, C. polykrikoides red tides seem to occur wherever the ground sea water contains materials that are needed for its growth.

• Journal of the Korean Society for Railway
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• v.10 no.6
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• pp.758-765
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• 2007

In this paper, we propose an efficient seamless handoff scheme to minimize packet loss and unnecessary packets on the Internet using the peculiar mobility characteristics of public vehicles such as trains. MR (Mobile Router) in a train visits each AR (Access Router) in the fixed order. As the MR detects reachability to the NAR (Next Access Router) on the new link, the PAR (Previous Access Router) can directly deliver packets from MR's HA (Home Agent) to the NAR according to the HML (Handoff Mobile router List). Then. the NAR buffers them until the MR finishes L3 (Layer 3) handoff procedure with the NAR. Therefore, our scheme can support a seamless handoff without the packet loss and unnecessary packets on the Internet. The result of our performance evaluation has shown that the proposed scheme could provide excellent performance, compared with the NEMO basic support protocol and the Bi-casting protocol.

• 제어로봇시스템학회:학술대회논문집
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• 2003.10a
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• pp.2441-2444
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• 2003

This paper describes fabrication of a micro cell counter integrated with an oxygen micropump and Sephadex G-25 beads counting experiment. The device utilized a phototransistor, microwindow, and light source of microscope for beads detection. Microheater and microchannel were used for pumping and guiding of beads to the microwindow. Counting capability of the device was tested with a peristaltic pump and the measured signals (${\sim}10\;mV$) with oscilloscope showed peak shape when beads passed the microwindow. Pumping of beads by the oxygen micropump was carried out by heating paraffin, which enveloped manganese dioxide (catalyst), to trigger the decomposition of hydrogen peroxide into water and oxygen. It lasted for 5 min with $7\;{\mu}l$ of wt. 30 % hydrogen peroxide. Beads counting by oxygen micropump showed peaks ($2{\sim}20\;mV$) with $30\;{\mu}l$ of beads sample and the number of peaks by magnitude was acquired.

• Korean Journal of Agricultural Science
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• v.7 no.1
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• pp.33-37
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• 1980

To clarify the influence of ethylene on flowering and senescence of carnation flowers and the treatment of the floral preservative solution for reducing their ethylene production, bud-cut carnations (Dianthus caryophyllus L. cv. Coral) were sprayed with/without 500 ppm ethephon [(2-chloroethyl) phosphonic acid] and then held in distilled water or preservative solution (5% sucrose+50 ppm $AgNO_3$+300 ppm 8-hydroxyquinoline). 1. Ethylene inhibited to bloom carnation flowers because of causing sleepiness. 2. Carnation flowers were led to senescence after a lot of ethylene was produced by them. Therefore, ethylene seems to act as a trigger of senescence in carnation flowers. 3. The features of senescence in carnation flowers were different in accordance with the amount of ethylene produced by the flowers. 4. Exposure to ethylene decreased fresh weight, flower diameter, and water uptake of carnations. 5. The floral preservative extended vase life markedly by inhibiting ethylene production of cut flowers. The flowers exposed to lots of ethylene, however, were not restored even though they were held in the preservative solution.

• The KIPS Transactions:PartC
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• v.12C no.4 s.100
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• pp.551-558
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• 2005

In this paper, we implement the fast handovers for mobile IPv6 (FMIPv6) on Linux system. Due to its dependency on operations in layer-2 (L2), we have added some functions into the network driver to generate triggers as the mobile node moves. We design and implement the FMIPv6 functions divided into two parts as an access router and a mobile node. We compare the packet loss and delay of the FMIPv6 implementation during the handover period with those of the MIPv6 and investigate the performance improvement.

• Journal of the Korea Society of Computer and Information
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• v.14 no.9
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• pp.37-46
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• 2009

The movement of the MR loaded on the train is confined to the bidirectional movement along the rail. Therefore, the AR connected to the MR can use the address information of the neighboring ARs and configure CoA in advance before performing L2 and L3 handoff. The MR can acquire new CoA immediately from the present AR after the movement detection procedure. The performance analysis shows that the proposed scheme can provide CoA to the MR about 1.8(s) at minimum and 4.98(s) at maximum faster than the Stateless scheme because the proposed scheme does not carry out any additional CoA and DAD procedure unlike the Stateless scheme.

• The Transactions of the Korean Institute of Electrical Engineers D
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• v.52 no.11
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• pp.650-656
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• 2003

A high speed Viterbi decoder with a circularly connected 2-dimensional analog processing ceil array Is proposed. The proposed Viterbi .decoder has a 2-dimensional parallel processing structure in which an analog processing cell is placed at each node of a trellis diagram, the output column of the analog processing cells is connected to the decoding column, and thus, the output(last) column becomes a column right before the decoding(first) column. The reference input signal given at a decoding column is propagated to the whole network while Its magnitude is reduced by the amount of a error metric on each branch. The circuit-based decoding is done by adding a trigger signals of same magnitudes to disconnect the path corresponding to logic 0 (or 1) and by observing its effect at an output column (the former column of the decoding column). The proposed Viterbi decoder has advantages in that it is operated with better performance of error correction, has a shorter latency and requires no path memories. The performance of error correction with the proposed Viterbi decoder is tested via the software simulation.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.30 no.5B
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• pp.253-263
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• 2005

This paper proposed a mobile multicast technique to satisfy end-to-end QoS for various user requirements in mobile network environment. In order to provide seamless mobility, fast handoff technique was applied. By using L2 mobile trigger, it was possible to minimize remarkable amount of packet loss by delay occurred during handoff. To provide efficient multicast, concept of hierarchy was introduced to Xcast++, which results in a creation of HXcast++. HXcast++ optimized transfer path of multicast and reduced expensive multicast maintenance costs caused by frequent handoff. Suggestion of GMA (Group Management Agent) mechanism allows joining to group immediately without waiting IGMP Membership query during handoff. GMA mechanism will minimize the delay for group registration process and the resource usage due to delay of withdrawal process. And also use of buffering & forwarding technique minimized packet loss during generation of multicast tree. IntServ/RSVP was used to provide End-to-End QoS in local domain and DiffServ was used in global domain. To minimize reestablishment of RSVP session delay, extended HXcast++ control messages ware designed to require PATH message. HXcast++ proposed in this thesis is defined as multicast technique to provide end-to-end QoS and also to satisfy various user requirements in mobile network environment.

• Progress in Medical Physics
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• v.8 no.1
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• pp.3-15
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• 1997

Adrenal chromaffin cells secrete catecholamine in response to acetylcholine. The secretory response has absolute requirement for extracellular calcium, indication that $Ca^{2+}$ influx through voltage dependent $Ca^{2+}$ channel (VDCC) is the primary trigger of the secretion cascade. Although the existence of various types of $Ca^{2+}$ channels has been explored using patch clamp technique in adrenal chromaffin cells, the contribution of different types of $Ca^{2+}$ channels to catecholamine secretion remains to be established. To investigate the quantative contribution of different types of $Ca^{2+}$ channels to cate-cholamine secretion, $Ca^{2+}$ current($I_{Ca}$) and the resultant membrane capacitance increment($\Delta{C}_{m}$) were simultaneoulsy measured. Software based phasor detector technique was used to monitor $\Delta{C}_{m}$. After blockade of L type VDCC with nicardipine (1$\mu$M), $I_{ca}$ was blocked to 43.85$\pm$6.72%(mean$\pm$SEM) of control and the resultant ㅿC$_{m}$ was reduced ot 30.10$\pm$16.44% of control. In the presence of nicardipine and $\omega$-conotoxin in GVIA(l$\mu$M), an N type VDCC antagonist, $I_{ca}$ was blocked to 11.62$\pm$2.96% of control and the resultant $\Delta{C}_{m}$ was reduced to 26.13$\pm$8.25% of control. Finally, in the presence of L, N, and P type $Ca^{2\pm}$ channel antagonists(nicardipine, $\omega$-Conotoxin GVIA, and $\omega$-agatoxin IVA, respectively), $I_{ca}$ and resultant $\Delta{C}_{m}$ were almost completely blocked. From the observation of parallel effects of $Ca^{2+}$ channel antagonists on $I_{ca}$ and $\Delta{C}_{m}$, it was concluded that L, N, and also P type $Ca^{2+}$ channels served and $Ca^{2+}$ source for exocytosis and no difference was observed in their efficiency to evoke exocytosis amost L, N, and P type $Ca^{2+}$ channels.