• Title/Summary/Keyword: L14

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Regeneration and the Maturation Induction of Free-Living Gametophytes of a Kelp Saccharina sculpera (Phaeophyceae) (갈조류 개다시마 유리배우체의 재생 및 성숙 유도)

  • Yoo, Hyun Il;Lee, Ki Hyun;Kim, Soo Hong;Ha, Dong Soo;Hwang, Eun Kyoung
    • Korean Journal of Environmental Biology
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    • v.36 no.4
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    • pp.576-583
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    • 2018
  • Investigation of optimal temperature, irradiance and photoperiod conditions for free-living gametophytes of Saccharina sculpera for natural resources conservation and mass cultivation of endangered species in the eastern coast of Korea. Induction of regeneration and maturation of the free-living gametophytes of S. sculpera were cultured at temperatures (5, 10, 15, 20 and $25^{\circ}C$), irradiance (10, 20, 40, 60 and $80{\mu}mol\;photons\;m^{-2}\;s^{-1}$ and photoperiods (14:10, 12:12 and 10:14 h L:D). The female gametophyte were actively regenerated without reaching sexual maturity under $10^{\circ}C$, $20{\mu}mol\;photons\;m^{-2}\;s^{-1}$ and 12:12 h (L:D) conditions. In contrast, the conditions for male gametophytes were slightly different at $15^{\circ}C$, $10{\mu}mol\;photons\;m^{-2}\;s^{-1}$ and 12:12 h (L:D). The sexual maturation of female and male gametophytes took place under $15^{\circ}C$, $40{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 14:10 h (L:D) and $10^{\circ}C$, $20{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 10:14 h (L:D), respectively. These results provide basic information for controlling the regeneration and maturation of free-living gametophytes for conservation and utilization of S. sculpera.

Effects of Initial Shoot, Root Length, and Acclimating Substrates on Survival Rate of Plantlets Regenerated from Somatic Embryos of Larix kaempferi (일본잎갈나무 체세포배 유래 식물체의 초기 신초와 뿌리 길이, 순화용 기질이 생존율에 미치는 영향)

  • Lee, Na Nyum;Yun, A Young;Kim, Ji Ah;Kim, Tae Dong;Kim, Yong Wook;Han, Sim Hee
    • Journal of Korean Society of Forest Science
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    • v.109 no.4
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    • pp.413-420
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    • 2020
  • We analyzed the growth characteristics of each cell line and acclimating substrate of Larix kaempferi plantlets regenerated from somatic embryos, with the goal of increasing the survival rate during the acclimation phase. Somatic embryos from three embryogenic cell lines (L14-66, L16-18, and L17-B4) were used, and the acclimating substrates were commercial soils for Larix species (Larix-Soil) and horticultural corps (Hort-Soil), Elle-pot, and Peat-plug. The average initial shoot and root length was shortest in L14-66 and longest in L17-B4. The average survival rate by cell line was highest (87.0%) in L17-B4 and lowest (64.3%) for L14-66. Survival rates by substrate were highest in Elle-pot (88.5%) and Peat-plug (88.9%). The initial shoot length of the L14-66 plantlets was highly correlated with survival rates in the Larix-Soil (r = 0.852), Hort-Soil (r = 0.692), and Elle-pot (r = 0.867) substrates, but not in Peat-plug with high total nitrogen content. The initial shoot length of the L17-B4 plantlets was not correlated with the survival rate in any of the substrates. The initial root length of the L14-66 plantlets was highly related to survival rates in the Larix-Soil (r = 0.986), Elle-pot (r = 0.846), and Peat-plug (r = 0.802) substrates, and the survival rate of the plantlets was higher as the initial root length was longer. The initial root length of the L17-B4 plantlets was related to survival rate only in the Larix-Soil (r = 0.896) and Elle-pot (r = 0.696) substrates. In conclusion, to increase the survival rate of plantlets, root length should be considered over shoot length, and it is recommended to use substrates with high nitrogen content, such as Peat-plug, or to add fertilizer, during the acclimating process. In addition, in order to increase the survival rate, lines with high initial growth should be developed.

The Properties of L-lower Approximation Operators

  • Kim, Yong Chan
    • International Journal of Fuzzy Logic and Intelligent Systems
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    • v.14 no.1
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    • pp.57-65
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    • 2014
  • In this paper, we investigate the properties of L-lower approximation operators as a generalization of fuzzy rough set in complete residuated lattices. We study relations lower (upper, join meet, meet join) approximation operators and Alexandrov L-topologies. Moreover, we give their examples as approximation operators induced by various L-fuzzy relations.

AKARI INFRARED CAMERA OBSERVATIONS OF THE 3.3 ㎛ PAH FEATURE IN Swift/BAT AGNs

  • Castro, Angel;Miyaji, Takamitsu;Shirahata, Mai;Ichikawa, Kohei;Oyabu, Shinki;Clark, David;Imanishi, Masatoshi;Nakagawa, Takao;Ueda, Yoshihiro
    • Publications of The Korean Astronomical Society
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    • v.32 no.1
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    • pp.197-199
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    • 2017
  • Using the InfraRed Camera (IRC) on board the infrared astronomical satellite AKARI we study the ${3.3{\mu}m}$ polycyclic aromatic hydrocarbon (PAH) feature and its connection to active galactic nucleus (AGN) properties for a sample of 54 hard X-ray selected bright AGN, including both Seyfert 1 and Seyfert 2 type objects. The sample is selected from the 9-month Swift/BAT survey in the 14-195 keV band and all of the sources have known neutral hydrogen column densities ($N_H$). The ${3.3{\mu}m}$ PAH luminosity ($L_{3.3{\mu}m}$) is used as a proxy for star-formation (SF) activity and hard X-ray luminosity ($L_{14-195keV}$) as an indicator of the AGN power. We explore for possible difference of SF activity between type 1 (un-absorbed) and type 2 (absorbed) AGN. We use several statistical analyses taking the upper-limits of the PAH lines into account utilizing survival analysis methods. The results of our log($L_{14-195keV}$) versus log($L_{3.3{\mu}m}$) regression shows a positive correlation and the slope for the type 1/unobscured AGN is steeper than that of type 2/obscured AGN at a $3{\sigma}$ level. Also our analysis shows that the circum-nuclear SF is more enhanced in type 2/absorbed AGN than type 1/un-absorbed AGN for low $L_{14-195keV}$ luminosity/low Eddington ratio AGN, while there is no significant dependence of SF activity on the AGN type in the high $L_{14-195keV}$ luminosities/Eddington ratios.

Inhibitory Effects of 14 Plants from Mongolia and Myanmar on Lipid Accumulation in 3T3-L1 and HepG2 Cells (몽골과 미얀마 식물 14종의 3T3-L1 및 HepG2 세포에서 지질 축적 억제효과)

  • Kim, SukJin;Kim, Gun-Hee
    • Journal of the Korean Society of Food Culture
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    • v.36 no.1
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    • pp.130-142
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    • 2021
  • This study examined the antioxidative and lipid accumulation inhibitory effects of 14 plants from Mongolia and Myanmar on 3T3-L1 and HepG2 cells. The total phenolic and flavonoid contents (TPC and TFC) of 14 plant extracts were measured, and the antioxidative activities were analyzed using DPPH, ABTS, FRAP, and ORAC. After measuring the pancreatic lipase levels and performing the thiobarbituric acid assay, the degree of lipid accumulation was determined by lipid (Oil Red O) staining and triglyceride assay in 3T3-L1 and HepG2 cells. M. paniculate (259.43 mgGAE/g) and C. benghalensis (130.78 mgNAE/g) had the highest TPC and TFC, respectively, among the 14 plants. R. acicularis Lindl. had the highest antioxidant activity in DPPH. The ABTS, FRAP, and ORAC results showed that the antioxidant activity of 11 species was higher than that of the positive control. The pancreatic lipase inhibitory effect of C. angustifolium Scop. was reduced to 23.65% at 0.1 mg/mL, and the level of lipid peroxidation of C. abrorescens Lam. was 0.63 nmol/mg. Five selected plants inhibited the lipid accumulation and triglyceride content, respectively, in 3T3-L1 and HepG2 cells. These results provide scientific evidence for developing functional foods using 14 plants from Mongolia and Myanmar, which have antioxidant activities and lipid accumulation reduction effects.

Effects of Temperature, Photon Irradiance, and Photoperiod on the Growth of Embryos of Sargassum horneri in Laboratory Culture (괭생이모자반(Sargassum horneri) 유배의 생장에 미치는 온도, 광량, 광주기의 영향)

  • Kim, Nam-Gil
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.48 no.1
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    • pp.76-81
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    • 2015
  • The effects of temperature, photon irradiance, and photoperiod on the growth of Sargassum horneri embryos were examined for female plants collected at Chokpo in Tongyeoung, Gyeongnam Prefecture, Korea on 27 December 2011. Mature plant receptacles were detached, and fertilized eggs were cultured in the laboratory at temperatures of $5-25^{\circ}C$ with photon irradiances of $10-80{\mu}mol\;m^{-2}s^{-1}$ under 14L:10D and 10L:14D photoperiods. Germination and embryo growth were rapid at $20-25^{\circ}C$ and $40{\mu}mol\;m^{-2}s^{-1}$ under 14L:10D. The number of rhizoids in the germinated young thalli was high at high photon irradiances ($40-80{\mu}mol\;m^{-2}s^{-1}$) and $15-20^{\circ}C$. At $20^{\circ}C$ and 14L:10D, vigorous growth of main and lateral branches was observed; however, at $5^{\circ}C$ under both photoperiods, plant growth decreased markedly. In the present study, S. horneri grew well at high temperatures ($20-25^{\circ}C$) and high photon irradiances ($40-80{\mu}mol\;m^{-2}s^{-1}$) with a 14L:10D photoperiod.

Thermal Resistance and daf-16 Regulation of Fermented Zizyphus jujuba Fruits in Caenorhabditis elegans

  • Yu, Young-Bob
    • Korean Journal of Plant Resources
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    • v.33 no.6
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    • pp.645-650
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    • 2020
  • The mechanism of anti-aging of fermented jujube (Zizyphus jujuba fruits (ZJF)) was investigated using transgenic daf-16 and mev-1 strains of C. elegans. Jujube extracts fermented for 7 days (F7-ZJF) and 14 days (F14-ZJF) with Laetiporus sulphureus were treated to a NGM agar plate with 10-15 transgenic daf-16 and mev-1 strains of the synchronized age. There was no difference of lifespan between the drug-treated group (7-day fermented ex. (F7-zjf-200 ㎍/mL), 14-day fermented ex. (F14-zjf-200 ㎍/mL)) and the non-treatment group in both daf-16 and mev-1 strains. In the thermal stress experiment, F7-zjf-200 ㎍/mL showed a significant (t = 4.017) activity in thermal stress resistance with a 12% higher survival rate than the control group. In the survival test in H2O2, F7-zjf-200 ㎍/mL and F14-zjf-100 ㎍/mL have significant activity in oxidative stress resistance compared to the control group. This study indicates that life span expand of N2 strain of the jujube extract is related to the regulation of daf-16 and inhibition of mev-1 signal in C. elegans.

Comparison study of effects of magnetic therapy at Hap-Kok(LI4 ) on the thermal change of Chun-Choo(ST25) in man (합곡(合谷)(L14)의 자침(刺鍼)과 자석외첩(磁石外貼)이 천추(天樞)(S25) 부분(部分) 영역(領域)의 온도변화(溫度變化)에 미치는 영향(影響) 비교(比較))

  • Baek Tae-Ho;Park Ryoung-Jun
    • Journal of Korean Medical Ki-Gong Academy
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    • v.5 no.1
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    • pp.22-39
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    • 2001
  • This study is designed to compare the effect of a needle with the magnet on body. We took the skin temperature of the belly with digital infrared thermographic imaging while we sticked needle and apply magnets on L14. We made experiments on 40 healthy male volunteers for one month. We classified control group not acupuncture or magnet adhering(CON). acupuncture group on left and right L14(LA). and the permanent magnet group adhering to left and right(LM). And LM is divided into S-polar permanent magnet group(LMS) and N-polar permanent magnet group adhering to left and right L14(LMN). When we observed that temperature changed with time, the skin temperature of th belly in CON descended significantly but LM, LMS and LMN is not changed significantly. As mentioned above. we observed that the needles on L14 affected the change of temperature on the belly, and conjectured that the appliance of magnets had the same results. If the mechanism depends on the meridian of body and energy. we suppose that the appliance of magnets and needles has same effects.

Monitoring of Perfluorinated Compounds (PFCs) in the Seawater of the East and West Coast in Korea (동·서해안지역 해수에서 과불화 화합물의 모니터링)

  • Son, Jun-ho;Chung, Seon-Yong;Kwon, Bum Gun
    • Journal of the Korean GEO-environmental Society
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    • v.18 no.11
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    • pp.5-12
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    • 2017
  • Perfluorinated compounds (PFCs) as environmental pollutants are an important environmental issue. However, little is known on the PFCs monitoring of sea waters around estuaries on the East and West Coasts of Korea. This study shows the monitoring results of PFCs in sea waters in these Coasts. Among 10 PFCs selected in this study, concentrations of perfluorooctanesulfonate (PFOS), perfluorooctanoate (PFOA), perfluorobutane sulfonate (PFBS), and perfluorohexane sulfonate (PFHxS) in the East Coast were 9.6-50.7 ng/L (total mean${\pm}$standard deviation: $26.14{\pm}12.66ng/L$), 13.79-44.58 ng/L ($27.95{\pm}11.41ng/L$), limit of quantification (LOQ)-2.6 ng/L ($0.96{\pm}1.15ng/L$), and 2.95-11.05 ng/L ($4.25{\pm}2.57ng/L$), respectively. In the West Coast, concentrations of PFOS, PFOA, PFBS, and PFHxS were 27.66-51.71 ng/L ($36.27{\pm}7.79ng/L$), 8.97-22.53 ng/L ($14.47{\pm}4.25ng/L$), LOQ-2.27 ng/L ($1.63{\pm}0.93ng/L$), and 3.0-7.66 ng/L ($4.27{\pm}1.49ng/L$), respectly. Other PFCs were below LOQ. The result of this study provides the distribution pattern of PFCs for assessing environmental pollution in two coastal areas of Korea.

Lignocellulose Biodegradation and Interaction between Cellulose and Lignin under Sulfate Reducing Conditions (황산염 환원 조건에서 리그노셀룰로오스의 분해 및 리그닌과 셀룰로오스의 상호작용)

  • Ko, Jae-Jung;Kim, Seog-Ku;Shimizu, Yoshihisa
    • Journal of the Korea Organic Resources Recycling Association
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    • v.15 no.4
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    • pp.131-137
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    • 2007
  • In this study, the biodegradation test on lignocellulose under sulfate reducing conditions was carried out. In particular, the interaction between cellulose and lignin was investigated with various g-cellulose/g-lignin (C/L) ratios: 42.15, 4.59, 2.51, 1.14 and 0.7. It was shown that the rate of cellulose degradation decreased in proportion to the lignin content. Assuming first order degradation kinetics, the consequences of competitive inhibition were graphically shown for different C/L ratios. The relation between cellulose reduction rate and C/L ratio was expressed by logarithm function with a determination coefficient of 0.97. Lignocellulose reduction rate was also described as a logarithm function of C/L ratio showing a inhibition effect by lignin. In the mean time, the rate of lignin decomposition was higher at C/L ratio of 2.51 and 1.14 compared with C/L ratios of 4.59 and 0.7, indicating that excessive extra carbon source is not appropriate for lignin biodegradation.

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