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http://dx.doi.org/10.14578/jkfs.2020.109.4.413

Effects of Initial Shoot, Root Length, and Acclimating Substrates on Survival Rate of Plantlets Regenerated from Somatic Embryos of Larix kaempferi  

Lee, Na Nyum (Department of Forest Bio-resources, National Institute of Forest Science)
Yun, A Young (Department of Forest Bio-resources, National Institute of Forest Science)
Kim, Ji Ah (Department of Forest Bio-resources, National Institute of Forest Science)
Kim, Tae Dong (Department of Forest Bio-resources, National Institute of Forest Science)
Kim, Yong Wook (Department of Forest Bio-resources, National Institute of Forest Science)
Han, Sim Hee (Department of Forest Bio-resources, National Institute of Forest Science)
Publication Information
Journal of Korean Society of Forest Science / v.109, no.4, 2020 , pp. 413-420 More about this Journal
Abstract
We analyzed the growth characteristics of each cell line and acclimating substrate of Larix kaempferi plantlets regenerated from somatic embryos, with the goal of increasing the survival rate during the acclimation phase. Somatic embryos from three embryogenic cell lines (L14-66, L16-18, and L17-B4) were used, and the acclimating substrates were commercial soils for Larix species (Larix-Soil) and horticultural corps (Hort-Soil), Elle-pot, and Peat-plug. The average initial shoot and root length was shortest in L14-66 and longest in L17-B4. The average survival rate by cell line was highest (87.0%) in L17-B4 and lowest (64.3%) for L14-66. Survival rates by substrate were highest in Elle-pot (88.5%) and Peat-plug (88.9%). The initial shoot length of the L14-66 plantlets was highly correlated with survival rates in the Larix-Soil (r = 0.852), Hort-Soil (r = 0.692), and Elle-pot (r = 0.867) substrates, but not in Peat-plug with high total nitrogen content. The initial shoot length of the L17-B4 plantlets was not correlated with the survival rate in any of the substrates. The initial root length of the L14-66 plantlets was highly related to survival rates in the Larix-Soil (r = 0.986), Elle-pot (r = 0.846), and Peat-plug (r = 0.802) substrates, and the survival rate of the plantlets was higher as the initial root length was longer. The initial root length of the L17-B4 plantlets was related to survival rate only in the Larix-Soil (r = 0.896) and Elle-pot (r = 0.696) substrates. In conclusion, to increase the survival rate of plantlets, root length should be considered over shoot length, and it is recommended to use substrates with high nitrogen content, such as Peat-plug, or to add fertilizer, during the acclimating process. In addition, in order to increase the survival rate, lines with high initial growth should be developed.
Keywords
Larix kaempferi; somatic embryogenic cell; initial shoot length; initial root length; survival rate; peat-plug;
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