• Journal of the Korean Wood Science and Technology
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• v.50 no.6
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• pp.414-426
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• 2022

The Lauraceae family has commercial uses, such as in the food, pharmaceutical, and perfume industries. This study was conducted to investigate anti-asthmatic activity of essential oils from the seven species in the Lauraceae family. The essential oils were extracted from the leaves of seven species, and the chemical composition was investigated by gas chromatography-mass spectrometry. The major constituents of essential oils differed depending on the species, even if they belonged to the same family. The main constituents were camphor (89.09%) in Cinnamomum camphora, linalool (26.91%) in Cinnamomum cassia, 1,8-cineole (23.90%) in Cinnamomum japonicum, d-limonene (10.27%) and β-eudesmol (10.03%) in Lindera obtusiloba, δ-cadinene (13.85%) and α-phellandrene (11.57%) in Machilus japonica, cis-,trans-β-ocimene (13.80% and 12.06%) and elemol (11.46%) in Neolitsea aciculata, and cis-β-ocimene (37.94%) and sabinene (24.91%) in Neolitsea sericea. The anti-asthmatic activity of essential oils was investigated using the lipopolysaccharide-induced NCI-H292 cells. The relative expression levels of the pro-inflammatory cytokines [interleukin (IL)-1β and IL-6] and mucus gene (MUC5AC and MUC5B) were significantly reduced by essential oils from seven species in the Lauraceae family. Among the seven essential oils, the essential oil from L. obtusiloba had the most superior anti-asthmatic activity. These results suggest that the essential oil of L. obtusiloba leaves could be used as an agent to suppress mucus hypersecretion.

• Journal of Korean Society of Forest Science
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• v.96 no.1
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• pp.65-69
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• 2007

Essential oils which were isolated from leaves and twigs of Lindera obtusiloba Blume by steam distillation method were analyzed by the NIST and Wiley 6 Library method, using GC-MS. 24 components from the experiment were identified, and sesquiterpenoids were major of the components. The main constituents in the leaves were germacrene B (17.78%), ${\beta}-caryophyllene$ (17.50%), phytol isomaer (13.36%) and $(-)-{\beta}-elemene$ (11.07%), and the main in the twigs were ${\beta}-elemol$ (24.47%), ${\alpha}-cadinol$ (13.73%), camphor (9.83%) and ${\beta}-citronellol$ (9.54%). Acyclic diterpene such as phytone, phytol and phytol isomer were isolated for the first time in the leaves of L. obtusiloba.

• Food Science and Preservation
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• v.12 no.1
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• pp.68-74
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• 2005

This study was carried out to analyze to the flavor components of Lindera obtusiloba BL leaf teas by different processing methods. 81 kinds of flavor components in the masted tea, 78 in the roasted tea after steaming, 88 in the withered tea, 86 in the fermented tea, 72 in the steamed tea, and 89 in the air dried tea ware by GC/MS. Hydrocarbones in Lindera obtusiloba BL leaf teas were 45 kinds of $\beta-piepne$, and 16 kinds of alcohols such as Linalool, n-octanoal, phenyl acetaldehyde, $(-)-\alpha-terpineol$, elemol, and cholest-5-en-3-ol. 11 kinds of ketones sachas 2-ethyl-2- propyl-cyclohexanone, and 8 kinds of aldehydes sach as phenyl acetaldehyde, tetradecanal, 10-undecanal, 4-Bromo-2-methylbutanal were found. Esters were methyl 9,12,15-octadecatrienate, didodecyl phthalace, 1,2-benzenediccarbaboxy acid-bis (2-ethylhexyl)ester and phenols was 2,6-bis(1,1-dimethylethyl)-4-methyl-phenol.

• The Journal of Internal Korean Medicine
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• v.33 no.4
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• pp.405-417
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• 2012

Objectives : In the present study, anti-metastatic properties of the methanol extract of L. obtusiloba (MLO) were evaluated. Methods : To determine the effect of MLO on cancer metastasis, we checked matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) activities and expressions in B16F10 melanoma cells. In addition, we performed cell migration assay as well as invasion assay using Matrigel. Finally, we used an in vivo lung metastasis model to confirm the anti-metastatic activity of MLO. Results : 1. MLO showed potent inhibitory effects on MMP-2 and MMP-9 activities and expressions via down-regulation of activation of NF-${\kappa}B$ in B16F10 melanoma cells. 2. Melanoma cell migration and invasion were down-regulated by MLO treatment. 3. Not only in vitro model, but MLO also significantly suppressed lung metastasis in vivo. Conclusions : The present results indicate that MLO has strong inhibitory effect on cancer metastasis. Therefore, L. obtusiloba could be a valuable anti-metastatic agent.

• Food Science and Preservation
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• v.21 no.3
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• pp.427-433
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• 2014

Ethanol and hot water extracts were prepared from Lindera obtusiloba Blume (LO) and Zanthoxylum piperitum DC (ZP) and used to evaluate their antimicrobial activities and thermal stability against six foodborne pathogens (3 gram-positive and 3 gram-negative bacteria). The antimicrobial activities were assessed using the agar diffusion method, and the thermal stabilities of extracts were examined after heat treatment at 60, 70, 80, and $100^{\circ}C$ for 10 min. The zones of inhibition by the LO extract or the ZP extract of the tested microorganisms were in the range of 21-30 mm and 19-25 mm, respectively, at 100 mg/mL concentrations. The 60% ethanol extract and the hot water extracts from LO showed the strongest antimicrobial effects against MRSA and Staphylococcus aurues, respectively. For the extract from ZP, the strongest antimicrobial effect was shown against S. aurues by 60% ethanol, and the weakest antimicrobial effect was shown against E. coli by the hot water extracts. The ZP extracts showed that the gram-positive bacteria were more sensitive than gram-negative bacteria. For the thermal stability of the extracts, the antimicrobial effects stabilized after heat treatment. Overall, the data suggest that the extracts have a potential for application in various food products for which a natural antimicrobial additive is desired.

• Journal of Applied Biological Chemistry
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• v.54 no.3
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• pp.178-183
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• 2011

The stem woods of Lindera obtusiloba Blume were extracted in 80% aqueous methanol and the concentrated extract was partitioned with ethyl acetate (EtOAc), butanol (n-BuOH), and $H_2O$, successively. From the EtOAc and n-BuOH fractions, five compounds were isolated through the repeated silica gel, octadecyl silica gel, and Sephadex LH-20 column chromatographies. On the basis of spectroscopic data including mass spectrometry, IR, $^1H$-NMR, $^{13}C$-NMR, distortionless enhancement by polarization transfer, and two-dimensional-NMR gradient correlated spectroscopy (gCOSY), gradient heteronuclear single quantum correlation (gHSQC), gradient heteronuclear multiple bonding connectivity (gHMBC), the chemical structures of the compounds were determined as asarinin (1), (+)-catechin (2), (-)-epicatechin (3), hyperin (4), and nudiposide (5). Compounds 1 and 5 were isolated for the first time from the stem wood of L. obtusiloba Blume.

• The Korean Journal of Mycology
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• v.39 no.1
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• pp.1-6
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• 2011

This study was conducted to reveal the diversity of arbuscular mycorrhizal fungi (AMF) in Mt. Munan, based on the morphological and molecular characters. Soil and root samples were collected from the rhizosphere of host plants including Lindera obtusiloba, Stephanandra incisa, Styrax obassis and Symplocos sawafutagi and AMF were trap-cultured with Sorghum bicolor as a host plant in a greenhouse. The spores were extracted from the cultured soils and five species were identified using morphological and molecular characteristics; Acaulospora longula, A. mellea, Ambispora leptoticha, Gigaspora margarita and Paraglomus occultum. The distribution of AMF showed different trends according to host plants. The dominant AMF species were A. longula in L. obtusiloba, A. leptoticha in S. incisa, S. obassis and S. sawafutagi.

• Korean Journal of Environment and Ecology
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• v.8 no.2
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• pp.93-106
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• 1995

To investigate the forest structure in Ijontong Cholto area, twenty four plots were set up (each l0$\times$10m ) and surveyed. According to the analysis of ordination by DCA, community divided into 5 groups. The divided groups are Quercus serrata -Q. valiabilis community(I), Q. serrata - Pinus densiflora community(II), Q. mongolica - Lindera obtusiloba community(III), Carpinus laxiflora - Acer pseudo-sieboldianum community(IV) and Acer mono community(V). So the successional trends seems to be from P. densiflora through Q. serrata, Q. valiabilis, Q. mongolica to C. laxiflora, C. cordata, A. mono in canopy layer. And in the subtree and shrub layer, it was expected that from Rhododendron mucronulatum, Rh. schlippenbachii to A. pseudo-sieboldianum, L. obtusiloba. But some sites were likely to become Q. mongolica edaphic climax.

• The Korean Journal of Ecology
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• v.17 no.2
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• pp.120-130
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• 1994

Stages of vegetation development on talus area were studied to examine temporal changes in species composition and vegetation structure, and to elucidate the mechanism of early patch formation. While ground coverage of lichens, which may form substrate for moss colonization and mitigate the heat-stress on rocks, decreased gradully, coverage of mosses increased slightly during primary succession. Ecological role of mossess related with water retention in community may be very important not only at pioneer stage but also at later stages because of little soil development on this talus area. Species diversity and species richness increased during the early stages of succession. Parthenocis년 tricuspidata and Sorbaria sorbifolia var. stellipa dominated in liana stage, Ulmus davidiana for. suberosa and Lindera obtusiloba in shrub stage, and Fraxinus rhynchophylla and Actinidia arguta in subtree stage, however, was composed of mixed forest of several tree species. U. davidiana for. suberosa, L. obtusiloba, Securinega suffruticosa and Rhus chinensis were relatively important woody species in early patch forming process. The results, however, suggested that early establishment on talus area might be strongly associated with chance for safe-site because both pioneer species and later species could take part in early patch forming process.

• Biomolecules & Therapeutics
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• v.18 no.2
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• pp.205-210
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• 2010

Several studies have shown that plant-derived polyphenols reduce cardiovascular accidents in high-risk patients and the inhibition of platelet function may be responsible for part of this benefit. Lindera obtusiloba is widely used in traditional herbal medicine for the treatment of cardiovascular and inflammatory diseases. Therefore, the antiplatelet and antithrombotic activities of Lindera obtusiloba Extracts (LOE) on in vitro platelet aggregation, radical scavenging activity and in vivo murine pulmonary thrombosis were examined. LOE was able to directly scavenge the stable DPPH radical in a concentration-dependent manner and its $IC_{50}$ value was 3.9 ${\pm}$ 0.1 ${\mu}g$/ml. LOE significantly inhibited collagen- and ADP-induced platelet aggregation in a concentration-dependent manner and its $IC_{50}$ value is 0.9 ${\pm}$ 0.1 mg/ml and 0.4 ${\pm}$ 0.1 mg/ml respectively. The inhibitory effect of LOE was comparable to aspirin ($IC_{50}$ values were 1.0 ${\pm}$ 0.5 and 1.0 ${\pm}$ 0.7 mg/ml, respectively). Furthermore, oral administration of LOE suppressed the death of mice with pulmonary thrombosis induced by intravenous injection of collagen plus epinephrine. Taken together, our results suggest LOE may be a promising candidate for antithrombotic agent, and the antithrombotic effect of LOE may be due to, at least in part, antiplatelet activity.