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Effect of Lindera obtusiloba extract on cancer metastasis  

Yun, Hyuk (Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University)
Lee, Yong-Jae (College of Pharmacy, Woo-Suk University)
Seo, Hyun-Won (College of Pharmacy, Woo-Suk University)
Park, Kyoung-Jae (College of Pharmacy, Woo-Suk University)
Ko, Ha-Neul (Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University)
Cha, Dong-Seok (College of Pharmacy, Woo-Suk University)
Kwon, Jin (Dept. of Prosthetics and Orthotics, Korea National College of Rehabilitation and Welfare)
Jeon, Hoon (College of Pharmacy, Woo-Suk University)
Kim, Kang-San (Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University)
Publication Information
The Journal of Internal Korean Medicine / v.33, no.4, 2012 , pp. 405-417 More about this Journal
Abstract
Objectives : In the present study, anti-metastatic properties of the methanol extract of L. obtusiloba (MLO) were evaluated. Methods : To determine the effect of MLO on cancer metastasis, we checked matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) activities and expressions in B16F10 melanoma cells. In addition, we performed cell migration assay as well as invasion assay using Matrigel. Finally, we used an in vivo lung metastasis model to confirm the anti-metastatic activity of MLO. Results : 1. MLO showed potent inhibitory effects on MMP-2 and MMP-9 activities and expressions via down-regulation of activation of NF-${\kappa}B$ in B16F10 melanoma cells. 2. Melanoma cell migration and invasion were down-regulated by MLO treatment. 3. Not only in vitro model, but MLO also significantly suppressed lung metastasis in vivo. Conclusions : The present results indicate that MLO has strong inhibitory effect on cancer metastasis. Therefore, L. obtusiloba could be a valuable anti-metastatic agent.
Keywords
Lindera obtusiloba; matrix metalloproteinase (MMP); cancer metastasis;
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