• 제목/요약/키워드: L-continuous

검색결과 1,613건 처리시간 0.021초

Alcohol Production from Whey in Batch and Continuous Culture of Kluyveromyces fragilis.

  • Heo, Tae-Ryeon;Kim, Jong-Soo;So, Jae-Seong
    • Journal of Microbiology and Biotechnology
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    • 제4권4호
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    • pp.333-337
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    • 1994
  • In order to develop the whey beverage, we examined the optimum conditions for alcohol fermentation by Kluyveromyces tragilis ATCC 46537. The optimum conditions for alcohol production by K. fragilis ATCC 46537 were as follows; pH 4.5, $30^{\cir}C$, with a supplement of 50 g/l of lactose. To develop a continuous production of alcohol from whey, we compared batch fermentation with continuous iermentation in conjunction with UF system. Batch fermentation produced 11.0 g/l of alcohol, whereas pseudocontinuous and continuous fermentation with UF system produced 8.5 g/l of alcohol. To increase the alcohol production, we added 50 g/l of lactose to both fermentations. Batch fermentation with lactose supplement produced 15.7 g/l of alcohol and continuous fermentation with lactose supplement in conjunction with UF system produced 15.0 g/l of alcohol. These results clearly demonstrate that the UF system can be used to increase the alcohol production from whey, supplemented with exogenous lactose.

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에탄올 발효방법에 따른 음식물류 폐기물의 바이오에탄올 생산성 비교 (Comparison of Bio-ethanol Productivity Using Food Wastes by Various Culture Modes)

  • 강희정;리홍선;김용진;김성준
    • KSBB Journal
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    • 제25권5호
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    • pp.471-477
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    • 2010
  • In order to improve bio-ethanol productivity by various cultivation methods in this paper, the culture modes using food wastes, such as batch culture, high-cell-density fermentation, SSF (simultaneous saccharification and fermentation) by fill & draw, continuous culture by fill & draw were performed and their productivities were compared. SSFs by fill & draw were performed by continuous decompression using 1 L evaporator system, and by 10 L bioreactor without decompression. In addition, the continuous cultures by fill & draw mode using SFW (saccharafied food wastes) medium were performed by changes of 40% culture broth with intervals of 12 h (0.03 $h^{-1}$), 6 h (0.07 $h^{-1}$), 3 h (0.13 $h^{-1}$). Consequently, productivities of bio-ethanol were 2.52 g/L-h and 1.30 g/L-h in batch culture and high- cell-density fermentation, respectively. The productivities of SSF by fill & draw showed 2.24 g/L-h and 2.03 g/L-h in continuous decompression with 1 L evaporator and 10 L bioreactor without decompression, respectively. Also, the productivities in continuous culture by fill & draw modes showed 2.02 g/L-h, 4.07 g/L-h and 6.25 g/L-h by medium change with intervals of 12 h, 6 h, and 3 h, respectively. In conclusion, the highest ethanol productivity was obtained in the continuous culture mode by fill & draw with dilution rate of 0.13 $h^{-1}$.

Effect of Light/dark Cycles on Wastewater Treatments by Microalgae

  • Lee, Kwangyong;Lee, Choul-Gyun
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제6권3호
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    • pp.194-199
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    • 2001
  • Chlorella kessleri cultivated in artificial wastewater using diurnal illumination of 12h light/12h dark (L/D) cycles. The inoculum density was 10(sup)5 cells/mL and the irradiance in light cycle was 45$\mu$mol㎡s(sup)-1 at the culture surface. As a control culture, another set of flasks was cultivated under continuous illumination. Regardless of the illumination scheme, the total organic carbon (TOC) and chemical oxygen demand (COD) was reduced below 20% of the initial concentration within a day. However, cell concentration under the L/D lighting scheme was lower tan that under the continuous illuminating scheme. Thus the specific removal rate of organic carbon under L/D cycles was higher than that under continuous illumination. This result suggested that C. kessleri grew chemoorganotrophically in the dark periods. After 3 days, nitrate was reduced to 136.5 and 154.1mg NO$_3$-N/L from 168.1mg NO$_3$-N/L under continuous illumination and under diurnal cycles, respectively. These results indicate nitrate removal efficiency under continuous light was better than that under diurnal cycles. High-density algal cultures using optimized photobioreactors with diurnal cycles will save energy and improve organic carbon sources removal.

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Continuous Propionic Acid Production from Cheese Whey Using In Situ Spin Filter

  • Gupta, Achin;Srivastava, Ashok K.
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제6권1호
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    • pp.1-5
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    • 2001
  • The potential use of spin filter device to retain Propionibacterium acidipropionici in the bioreactor under continuous mode of fermentation, and improve acid productivity, was examined. The yield of propionic acid based on lactose concentration was 51% in batch and 54% in continuous (dilution rate = 0.05 h(sup)-1) operation. The yield in continuous fermentation with cell retention using spin filter of 10 micron size (dilution rate = 0.05 h(sup)-1) was even higher at 70% (w/w). The volumetric productivity under batch and continuous mode of operation were 0.312g L(sup)-1 h(sup)-1 and 0.718g L(sup)-1 h(sup)-1 respectively. Continuous fermentation with cell retention demonstrated even higher volumetric productivities at 0.98g L(sup)-1 h(sup)-1 with out clogging problems. It could be used for utilization of cheese whey to produce propionic acid at higher yield and productivities.

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ON L-FUZZY ALMOST PRECONTINUOUS FUNCTIONS

  • Min, Won-Keun
    • 한국수학교육학회지시리즈B:순수및응용수학
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    • 제3권1호
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    • pp.53-58
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    • 1996
  • In 1981, R . Badard introduced the notion of fuzzy pretopological spaces and their representation[1]. And in 1992, R. Badard, et al. introduced the L-fuzzy pretopological spaces and studied properties of continuity, open map, closed map, and homeomorphism in L-fuzzy pretopological spaces. In this paper we introduce and study the concepts of almost continuous functions and weakly pre-continuous functions on L-fpts's.(omitted)

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글리세롤 기반의 바이오에탄올 생산을 위한 연속생산반응기의 성능 비교 (Performance Comparison of Continuous Reactors for Bioethanol Production Based on Glycerol)

  • 이상준;송윤석;김성봉;강성우;한성옥;박철환;김승욱
    • KSBB Journal
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    • 제26권4호
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    • pp.328-332
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    • 2011
  • Ethanol production using glycerol as a carbon source was performed by Enterobacter aerogenes immobilized on calcium alginate beads. To improve the ethanol production, the optimal conditions such as loading amount of immobilized cells and glycerol concentration were investigated. The optimal loading amount of immobilized cells and glycerol concentration were 10 mL of calcium alginate bead and 10 g/L, respectively. Consequently, glycerol consumption rate, ethanol concentration and yield were 0.32 g/$L{\cdot}h$, 3.38 g/L and 0.43 g/g on the batch production, respectively. Continuous production of ethanol was successfully achieved using two types of immobilized cell reactors (continuous stirred tank reactor and packed bed reactor) from 10 g/L of glycerol. In the continuous stirred tank reactor, glycerol consumption, ethanol concentration, specific productivity and yield were 9.8 g, 4.67 g/L, 1.17 g/$L{\cdot}h$, 0.48 g/g, respectively. The concentration of produced ethanol was 38-44% higher comparison to batch fermentation, and continuous stirred tank reactor showed better performance than packed bed reactor.

The Effects of Propofol and Thiopental Continuous Infusion on Serum Potassium Disturbances in Neurosurgical Patients

  • Kim, Tae Kyong;Lim, Young-Jin;Ju, Jae-Woo;Kim, Jin Wook;Park, Hee-Pyoung
    • Journal of Korean Neurosurgical Society
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    • 제57권3호
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    • pp.197-203
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    • 2015
  • Objective : The potassium disturbance associated with thiopental continuous infusion in neurosurgical patients is well known. However, the effect of propofol continuous infusion on serum potassium levels has not been investigated extensively. Methods : We reviewed the medical records of 60 consecutive patients who received coma therapy or deep sedation for intracranial pressure control using either thiopental or propofol between January 2010 and January 2012. Results : The overall incidence of hypokalemia (K<3.5 mmol/L) was comparable between thiopental and propofol groups (89.2% vs. 82.6%). But, the incidence of moderate to severe hypokalemia (K<3.0 mmol/L) was significantly higher in thiopental group (51.4% vs. 13.0%, p=0.003). The lowest potassium level (2.9 mmol/L vs. 3.2 mmol/L, p=0.020) was lower in thiopental group. The patients in the thiopental group required greater potassium replacement than the propofol group patients (0.08 mmol/kg/h vs. 0.02 mmol/kg/h, p<0.001). On multivariate analysis, thiopental [odds ratio, 95% confidence interval, 7.31 (1.78-27.81); p=0.005] was associated with moderate to severe hypokalemia during continuous infusion. The incidence of rebound hyperkalemia (K>5.0 mmol/L, 32.4% vs. 4.3%, p=0.010) and the peak potassium concentration (4.8 mmol/L vs. 4.2 mmol/L, p=0.037) after the cessation of therapy were higher in thiopental group. On multivariate analysis, thiopental [8.82 (1.00-77.81); p=0.049] and duration of continuous infusion [1.02 (1.00-1.04); p=0.016] were associated with rebound hyperkalemia once therapy was discontinued. Conclusion : Propofol was less frequently associated with moderate to severe hypokalemia after induction and rebound hyperkalemia following the cessation of continuous infusion than thiopental.

A continuous time asymmetric power GARCH process driven by a L$\'{e}$vy process

  • Lee, Oe-Sook
    • Journal of the Korean Data and Information Science Society
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    • 제21권6호
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    • pp.1311-1317
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    • 2010
  • A continuous time asymmetric power GARCH(1,1) model is suggested, based on a single background driving L$\'{e}$vy process. The stochastic differential equation for the given process is derived and the strict stationarity and kth order moment conditions are examined.