• KSBB Journal
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• v.7 no.4
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• pp.296-301
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• 1992

A Pseudomonas fluorescens was selected from mushrooms and studied in both batch and fed-batch cultures in order to get maximal biomass concentration. P. fluorescens is an aerobic bacterium and antagonistic to Pseudomonas tolaasii which causes blotch disease on the mushroom cap. P fluarescens and P. tolaasii were identified by Gram staining, gelatin liquefaction, oxidase test, etc. and were characterized by pigment production, temperature sensitivity, salt tolerance and rapid pitting test, etc., Celts of P. fluorescens well in medium containing 30g/L of glucose, whereas the growth was inhibited at the glucose levels at higher than 30g/L. The highest values of specific growth rate and productivity were obtained when using 10g/1 of yeast extract. Optimum concentrations of $NH_4Cl$ and ${(NH_4Cl)}_2SO_4$ for culture were found to be 1.0g/L and 0.1g/L respectively. Optimum concentration of $MgSO_4{\cdot}7H_2O$ used as a sulfursource was 1.0g/L. It was also found that the cell concentrations reached the maximum level when grown on the medium containing 1.0g/L of $KH_2PO_4$ and 0.1g/L of $CaCl_2$. Also, the optimum culture conditions were $30^{\circ}C$ and pH 6.0. Cultivation of P. fluarescens at high dissolved oxygen (DO) concentration led to a decrease of bacterial productivity in batch culture. Maximum productivity was achieved at 40% DO concentration.

• Korean Journal of Plant Resources
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• v.22 no.5
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• pp.454-460
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• 2009

Phyllanthus urinaria was an important species in Korea and distributed in all around of Korea. The roots and stems of this plant have been used for natural medicine for the treatment of diabetes, the hepatitis B virus and disturbances of the kidney and urinary bladder. Production of adventitious roots in P. urinaria by in vitro cultures could be used as alternatives materials. Shoot and root segments from P. urinaria seedling were cultured on Murashige and Skoog (MS) medium supplemented with 3.0 mg/L IBA and 30 g/L sucrose. After 4 weeks of culture, the highest induction of adventitious roots was obtained from the shoot part. Frequency of adventitious root formation on medium with various kinds of auxins (IAA, NAA, 2,4-D, and IBA) and various concentrations of IBA (0, 0.1, 0.5, 1.0, 3.0, and 5.0 mg/L) was tested. The maximun induction of adventitious root was obtained on medium with 0.5 mg/L IBA. In liquid culture, growth of root was best on medium supplemented with 30 g/L sucrose. Adventitious roots were cultured in 5 L bioreactor containing 1/2 MS medium supplemented with 0.5 mg/L IBA and 30 g/L sucrose and mass-production of adventitious roots was successfully achieved. These results revealed the first attempt for the production of adventitious roots in P. urinaria.

• Food Science of Animal Resources
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• v.25 no.1
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• pp.92-97
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• 2005

This study was carried out to determine the storage, cryotolerance, heat and drying resistance, when Lactobacillus acidophilus CT 01 isolated from preweaned piglet feces growing at suboptimal temperature. L. acidophilus CT 01 suboptimal temperature incubated for 48 hours had the slowest growth rate at 22℃ but the highest viable cell number after 36 hours at 22℃, with 1.3×10/sup 9/ CFU/mL. In case of 4 and 20℃ storage, the suboptimal temperature incubated groups had a viability higher than the control (p<0.01). The cryotolerance of suboptimal temperature incubated L. acidophilus CT 01 was a higher than the control (p<0.01). When L. acidophilus CT 01 was heat treated at 60℃ for 15 minutes and 30 minutes, the suboptimal temperature incubated L. acidophilus CT 01 at 22℃ had a viability higher more than the control (p<0.01). L. acidophilus CT 01 incubated suboptimal temperature was inoculated by 30% to the carrier, and dried at 50℃ for 12 hours had the highest viability in the suboptimal temperature incubated L. acidophilus CT 01 at 28℃.

• The Korean Journal of Food & Health Convergence
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• v.4 no.2
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• pp.7-11
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• 2018

Salinity is one of the major and increasing problems in irrigated agriculture in Pakistan. Salinity stress negatively affects the growth and yield of plants guar (Cyanoposisa tetragonoloba). This experiment was conducted to evaluate the effects of ($4dSm^{-1}+13.5(mmol\;L^{-1})^{1/2}$, $5dSm^{-1}+25(mmol \;L^{-1})^{1/2}$, $5dSm^{-1}+30(mmol\;L^{-1})^{1/2}$, $10dSm^{-1}+25(mmol\;L^{-1})^{1/2}$ and $10dSm^{-1}+30(mmol \;L^{-1})^{1/2}$) on biomass yield of guar against salinity tolerance. Maximum biomass yield ($54.50gpot^{-1}$) was produced by $4dSm^{-1}+13.5(mmol\;L^{-1})^{1/2}$ treatment. Biomass produce was reduced with the increase of the salts toxicity. Minimum biomass yield ($30.17gpot^{-1}$) was attained under $10dSm^{-1}+30(mmol \;L^{-1})^{1/2}$. $5dSm^{-1}+25(mmol\;L^{-1})^{1/2}$ treatment exhibited improved outcome i.e. the least diminution % over control (18.66). Salinity cum sodicity showed staid effect on the growth reduction from 18.66% to 44.64%. This reduction fissure was impacted by the toxic effect of salinity and sodicity on Guar growth. Salinity- sodicity behaved toxic impact on the growth reduction from 18.66% to 44.64%. Based on the findings, guar (Cyanoposisa tetragonoloba) grows better at $4dSm^{-1}+13.5(mmol \;L^{-1})^{1/2}$ treatment.

• YAKHAK HOEJI
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• v.55 no.6
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• pp.510-515
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• 2011

Isoquinoline compounds including berberine enhance L-DOPA-induced cytotoxicity in PC12 cells. In this study, the effects of berberine on L-DOPA therapy in unilateral 6-hydroxydopamine (6-OHDA)-induced rat models of parkinsonism were investigated. Rats were prepared for the models of Parkinson's disease by 6-OHDA-lesioning for 14 days and then treated with L-DOPA (10 mg/kg) with or without berberine (5 and 30 mg/kg, i.p.) for 21 days. Treatment with berberine (5 and 30 mg/kg, i.p.) showed a dopaminergic cell loss in substantia nigra of 6-OHDA-lesioned rats treated with L-DOPA: 30 mg/kg berberine was more intensive neurotoxic. The levels of dopamine were also decreased by berberine (5 and 30 mg/ kg) in striatum-substantia nigra of 6-OHDA-lesioned rats treated with L-DOPA. These results suggest that berberine aggravates cell death of dopaminergic neurons in L-DOPA-treated 6-OHDA-lesioned rat models of Parkinson's disease. Therefore, the long-term L-DOPA therapeutic patients with isoquinoline compounds including berberine may need to be checked for the adverse symptoms.

• Applied Biological Chemistry
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• v.39 no.3
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• pp.172-176
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• 1996

Effect of culture conditions such as pH, temperature, agitation speed and oxygen transfer rate on xylitol production from xylose by Candide parapsilosis ATCC 21019 mutant was investigated in a jar fermentor. The initial concentration of xylosr was fixed at 50 g/l in this experiment. When pH was increased, cell growth and xylose consumption rate were increased, but maximum xylitol production was shown in the range of pH 4.5 and 5.5 with a yield of 0.68 g/g-xylose. The optimal temperature for xylitol production was determined to be $30^{\circ}C$. Considering the importance of dissolved oxygen tension, for xylitol production, the effect of oxygen transfer rate coefficient $(k_La)$ on fermentation parameters was carefully evaluated in the range of $20{\sim}85\;hr{-1}\;of\;k_La$ (corresponding to $100{\sim}300$rpm of agitation speed). The xylitol production was maximized at $30\;hr^{-1}\;of\;k_La$(150 rpm). A higher oxygen transfer rate supported better cell growth with lower xylitol yield. It was determined that maximum xylitol concentration, xylitol yield and productivity was 35.8 g/l, 71.6% and $0.58\;g/l{\sim}hr^{-1}$, respectively, at $30\;hr^{-1}\;of\;k_La$ In order to further increase xylitol productivity, ferementation using the concentrated biomass(20 g/l) was carried out at the conditions of pH 4.5, $30^{\circ}C$ and $30\;hr\;1$ of oxygen transfer rate. The final xylitol concentration of 40 g/l was obtained at 18 hours of culture time. From this result, it was calculated that xylitol yield was 80ft on the basis of xylose consumption and volumetric productivity was $2.22\;g/l{\sim}hr$ which was increased by $3{\sim}4$ fold compared with $0.5{\sim}0.7\;g/l-hr$ obtained in a normal fermentation condition.

• Food Science and Preservation
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• v.15 no.1
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• pp.58-65
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• 2008

The Acorn (Quercus acutissima CARRUTHERS), which contains a large quantity of tannin, should be developed as a processed food as the acorn is rich in natural antioxidants and other valuable components. Accordingly, acorn extraction conditions for polyphenol and gallic acid (both antioxidants) were investigated by response surface methodology. The content of polyphenols were determined under 16 different extraction conditions based upon a central composite design. The parameters varied over $30-70^{\circ}C$ of extraction temperature, 1-5 h of extraction time, and 5-25 mL/g of solvent ratio, Gallic acid extraction was optimal at $60-100^{\circ}C$ extraction temperature, 1-5 h of extraction time, and 5-25 mL/g of solvent ratio, Epicatechin content was highest at $56.77^{\circ}C$, 4.16 hand 22.38 mL/g. Catechin content was highest at $52.37^{\circ}C$, 2h and 23.59 mL/g. The maximum catechin content was $91.30{\mu}g/mL$. Epigallocatechin content was influenced by extraction temperature and time. The maximum epigallocatechin content was $1,066.56{\mu}g/mL$ at $61.42^{\circ}C$, 4.17h, and 9.25 mL/g. The maximum value of epicatechingallate content was $125.39{\mu}g/mL$ at $47.72^{\circ}C$, 3.04h, and 24.93mL/g. Epigallocatechingallate content was influenced principally by solvent ratio and the maximum content was $61.38{\mu}g/mL$ at $48.11^{\circ}C$, 2.96h, and 24.95mL/g. The total polyphenol content was maximal at $1,332.75{\mu}g/mL$, after extraction at $61.50^{\circ}C$, 4.24h, at 9.71mL/g. The higher the extraction temperature and the longer the extraction time, the greater the polyphenol content. Gallic acid content was highest, the maximal level was $30.51{\mu}g/mL$ after $65.84^{\circ}C$, 1.65h at 17.17 mL/g, and this was influenced principally by extraction time and solvent ratio.

• Journal of Life Science
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• v.19 no.12
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• pp.1836-1840
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• 2009

Recently, hydroxymethylfurfrual (HMF) has been highlighted as a key intermediate for the production of liquid biofuels and other valuable compounds. We used sesame roots as a biocatalyst to synthesize HMF using flask cultures. The synthesis of HMF was identified by GC-mass analysis. The highest root growth was observed in cultures with 1.0 mg/l NAA at $30^{\circ}C$, while root growth was not found in those without NAA treatment. When silver nitrate ($AgNO_3$) was added, the root growth was greatest in those treated with 0.5 mg/l $AgNO_3$ and cultured at $30^{\circ}C$. In the case of HMF synthesis, its highest yield was obtained in those treated with 0.5 mg/l NAA at $25^{\circ}C$, but low HMF was detected in those treated without naphthaleneacetic acid (NAA). The addition of $AgNO_$ to the culture medium showed a 8-10% reduction in HMF yield compared to that of the control, indicating its inhibitory effect on the synthesis of HMF. On the whole, an optimal culture temperature for HMF synthesis seemed to be between $25-30^{\circ}C$.

• Korean Journal of Environmental Agriculture
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• v.27 no.4
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• pp.356-361
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• 2008

Chrysanthemum indicum L. (Gamgug) has been examined to study their flowering habits, yields and bioactive compounds under different planting densities and mowing dates. The planting density experiment revealed a significantly increasing stem diameter, number of flowers and branches with decreasing plant density in the $100\;cm{\times}30\;cm$ and $130\;cm{\times}30\;cm$ treatments as compared to $70\;cm{\times}30\;cm$ treatments, but not plant height, leaf and flower width. On the other hand, the mowing date experiment showed that growth characteristics of plants were similar to the control plants (not mowing) and June 20 treatment, but July 20 treatments had significantly smaller than the control. The weights (g $plant^{-1}$) of dry flowers were affected by the planting density and mowing date. The flower yield of $586\;kg\;ha^{-1}$ obtained at $100\;cm{\times}30\;cm$ density was 11% and 22% higher than that of $120\;cm{\times}30\;cm$ and $70\;cm{\times}30\;cm$ treatments, respectively. The yield of dry flowers in the control and June 20 mowing date ranged $495-508\;kg\;ha^{-1}$, which is 40-42% higher than the yield in the July 20 treatments. The amount of essential oil (g $plant^{-1}$) in medically valuable flowerheads of C. indicum L. was statistically different between mowing dates but not among planting densities. The study showed that planting density and the mowing date could increase yields of flowerheads. An optimum planting density of $100\;cm{\times}30\;cm$ and mowing date of on or before June 20 is recommended for C. indicum L.

• Mycobiology
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• v.30 no.3
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• pp.128-132
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• 2002

Eight species belonging to two genera of the Laboulbeniales, parasitic on the family Carabidae and Staphylinidae of the order Coleoptera, were collected from Upo natural swamp in 1996-2001. They are as follows; Laboulbenia anoplogenii parasitic on Platynus daimio, L. cristata on Paederus parallelus, L. egens on Tachys laetifica, L. flagellata on Platynus daimio, L. pedicellata on Bembidion morawitzi, L. philonthi on Philonthus wuesthoffi, L. stenolophi on Acupalpus inornatus and Philonthus longicornis, and Peyritschiella biformis on Philonthus micaticollis. Among these species, the genus Platynus as the host of L. anoplogenii and the genus Acupalpus as the host of L. stenolophi are new to science. Bembidion morawitzi as the host of Laboulbenia pedicellata and Philonthus micanticollis as the host of Peyritschiella biformis are new to Korea.