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Induction and in vitro Proliferation of Adventitious Roots in Phyllanthus urinaria  

Bae, Kee-Hwa (Jeju Biodiversity Research Institute Jeju Hi-Tech Industry Development Institute)
Yun, Pil-Yong (Jeju Biodiversity Research Institute Jeju Hi-Tech Industry Development Institute)
Choi, Yong-Eui (Division of forest resources, college of forest and environmental sciences, Kangwon National University)
Publication Information
Korean Journal of Plant Resources / v.22, no.5, 2009 , pp. 454-460 More about this Journal
Abstract
Phyllanthus urinaria was an important species in Korea and distributed in all around of Korea. The roots and stems of this plant have been used for natural medicine for the treatment of diabetes, the hepatitis B virus and disturbances of the kidney and urinary bladder. Production of adventitious roots in P. urinaria by in vitro cultures could be used as alternatives materials. Shoot and root segments from P. urinaria seedling were cultured on Murashige and Skoog (MS) medium supplemented with 3.0 mg/L IBA and 30 g/L sucrose. After 4 weeks of culture, the highest induction of adventitious roots was obtained from the shoot part. Frequency of adventitious root formation on medium with various kinds of auxins (IAA, NAA, 2,4-D, and IBA) and various concentrations of IBA (0, 0.1, 0.5, 1.0, 3.0, and 5.0 mg/L) was tested. The maximun induction of adventitious root was obtained on medium with 0.5 mg/L IBA. In liquid culture, growth of root was best on medium supplemented with 30 g/L sucrose. Adventitious roots were cultured in 5 L bioreactor containing 1/2 MS medium supplemented with 0.5 mg/L IBA and 30 g/L sucrose and mass-production of adventitious roots was successfully achieved. These results revealed the first attempt for the production of adventitious roots in P. urinaria.
Keywords
bioreactor; IBA (indole-3-butyric acid); P. urinaria;
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