• Title/Summary/Keyword: Kwon Seop

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Intraspecific variations in macronutrient, amino acid, and fatty acid composition of mass-cultured Teleaulax amphioxeia (Cryptophyceae) strains

  • Lee, Bae Ik;Kim, Shin Kwon;Kim, Jong Hyeok;Kim, Hyung Seop;Kim, Jong Im;Shin, Woongghi;Rho, Jung-Rae;Yih, Wonho
    • ALGAE
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    • v.34 no.2
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    • pp.163-175
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    • 2019
  • To compare the nutritional quality of TPG (Teleaulax / Plagioselmis / Geminigera) clade species of cryptomonads with that of RHO (Rhodomonas / Rhinomonas / Storeatula) clade species 6 Teleaulax amphioxeia (TA) and 1 Rhinomonas sp. strains were mass-cultured in newly designed 500-L photobioreactors to the end of exponential growth phase. Intraspecific variations (IVs) in terms of one standard deviation among the 6 TA strains in the compositions of the three macronutrients were 41.5 (protein), 89.8 (lipid), and 15.6% (carbohydrate) of the mean. When harvested from stationary growth phase mean compositions of essential amino acids (EAAs, 47.3%) and non-EAAs (52.7%) of the 2 TA strains, CR-MAL07 and CR-MAL08-2, were similar to those of a Chroomonas strain. The IVs between the 2 TA strains in the composition of EAAs (10.3 and 2.4) and non-EAAs (8.5 and 2.1% of the mean) were rather smaller than those of saturated fatty acids (30.3 and 26.1) and unsaturated fatty acids (UFAs, 12.0 and 12.5% of the mean) in f/2-Si and urea-based compound fertilizer (UCF) culture media, respectively. Mean compositions of eicosapentaenoic acid (EPA, 17.9%) and docosahexaenoic acid (DHA, 12.7%) of total fatty acids of the 2 TA strains were higher than those that of a Chroomonas strain. EPA and DHA compositions exhibited similar level of IVs between the 2 TA strains in f/2-Si (14.6 and 11.0) and UCF media (12.6 and 13.5% of the mean). Thus, the nutritional quality in terms of amino acids, UFAs, EPA, and DHA in a TPG clade species, T. amphioxeia was comparable to those of RHO clade species with notable IVs. Practically, biotechnological targets for TPG clade cryptomonad strains might be subspecies or clone level.

Study on the Status of Application of Trade Secrets in MSDS Provided in Workplaces (산업체 규모와 업종에 따른 MSDS 영업비밀 적용 실태조사 연구)

  • Lee, Kwon Seob;Choi, Heung Koo;Lee, In Seop
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.29 no.1
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    • pp.27-33
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    • 2019
  • Objective: This study analyzed the status of the application of trade secrets in MSDSs according to size and type of industry. The contents of the MSDS non-public approval policy are summarized. We suggest proactive improvement requirements related to the operation of the MSDS non-public approval policy. Methods: To review this subject, we selected 153 manufacturers and six importers in such fields as organic chemical production. The trade secrets application status and ratio (%) of MSDSs by industry size and industry classification were investigated. Improvements toward a proactive system related to the operation of the MSDS non-public approval policy under the Occupational Safety and Health Act(OSHA) were summarized. Results and Conclusions: According to the results, the trade secret ratio in MSDSs by industry size was 33% in workplaces with less than 50 employees, 23.1% in workplaces with more than 50 but less than 100 workers, 73% in workplaces with more than 100 workers, and 83.4% in workplaces with 300 or more workers. For the trade secret writing rate for MSDSs by industry, the highest was MOCCP (Manufacture of ink, paint, coating and similar products) at 80.9%. MOC (Manufacture of other chemicals) was the lowest at 16.2%. We propose four proactive efforts to minimize the administrative burden of implementation of the MSDS non-public approval policy. The results of this study can be used as basic data for policy improvements to make more effective use of MSDS.

A Study on 3D File Format for Web-based Scientific Visualization

  • Lee, Geon-hee;Nam, Jeong-hwan;Han, Hwa-seop;Kwon, Soon-chul
    • International Journal of Advanced Culture Technology
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    • v.7 no.1
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    • pp.243-247
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    • 2019
  • The most commonly used 3D modeling file formats are OBJ (Wavefront file format specification) and STL (STereoLithography). Although they have a common point of view in 3D on the screen, detailed functions are different according to purpose of development. OBJ is the most commonly used 3D file format and STL is mainly used as 3D file format for 3D printing. However, in the field of Scientific Visualization, precise analysis is required. There is a difference in accuracy depending on the type of 3D file format. OBJ and STL are not suitable for delicate surface description because they form meshes in the form of triangular polygons. And if you increase the number of triangle polygons, it will be smoother, but the file size also increases exponentially and causes excessive CPU usage. In contrast, VTK provides a variety of polygon structures, including triangular polygons as well as rectangular polygons and cube polygons. Thus, delicate surface depiction is possible. Delicate surface rendering is possible and file size is not large. This paper describes the concept and structure of VTK. We also compared the load times and file sizes between VTK, STL, and OBJ in the Chrome browser. In addition, the difference in surface rendering ability between VTK, STL, and OBJ is intuitively viewed based on the screen in which each 3D file format is implemented under the same conditions. This study is expected to be helpful for efficient 3D file format for precise implementation of Web - based Scientific Visualization.

Characteristics of Cyanobacteria and Odorous Compounds Production in Lake Uiam and Lower Gonji Stream (의암호와 공지천 하류에서 남조류와 냄새물질의 발생 특징)

  • Youn, Seok Jea;Im, Jong Kwon;Byeon, Myeong-Seop;Yu, Soon Ju
    • Journal of Korean Society on Water Environment
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    • v.35 no.2
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    • pp.99-104
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    • 2019
  • The objective of this study was to investigate the relationship between the biomass of cyanobacteria and the concentration of 2-methylisoborneol (2-MIB) in the lower Gongji stream. The investigation was done using a field study that was conducted from 2015 to 2017. The 2-MIB concentration in the lower Gongji stream ranged from 0 to 153 ng/L, while the upper stream had 0 2-MIB concentration. 3 genera (Oscillatoria, Phormidium, Pseudanabaena) of cyanobacteria were detected in the lower Gongji stream with 2-MIB concentration. Among these 3 genera, an increase in Phormidium, Pseudanabaena biomass was associated with an increase in 2-MIB concentration. Accordingly, Phormidium, Pseudanabaena were regarded as the biological source of 2-MIB in that area. In October 2017, although planktonic cyanobacteria occurred less frequently, many benthic cyanobacteria mats were observed on the surface of the water body. Therefore, the high 2-MIB concentration, which exceeded 110 ng/L, can likely be attributed to the benthic cyanobacteria. In a laboratory experiment, individual Oscillatoria filaments were aggregated to form a colony with a higher density. This colony tended to float on the water surface. Cyanobacteria mats after floating aggregated mats were distributed in a net shape on the bottom.

Evaluation of Water Quality and Phytoplankton Community Using a Multivariate Analysis in Bukhan River (다변량 통계분석을 이용한 북한강의 수질 및 식물플랑크톤 군집 특성 평가)

  • Kim, Hun Nyun;Youn, Seok Jea;Byeon, Myeong Seop;Yu, Soon Ju;Im, Jong Kwon
    • Journal of Korean Society on Water Environment
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    • v.35 no.1
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    • pp.19-27
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    • 2019
  • The purpose of this study is to evaluate the water quality and phytoplankton community in Bukhan River which account for 44.4 % of the total inflow into Lake Paldang, using multivariate statistical techniques (i.e., correlation analysis, principal component analysis (PCA)/factor analysis (FA)). Water samples were collected from March to November 2015 and the following parameters measured; water temperature, pH, DO, EC, SS, BOD, Chl-a, COD, TN, $NO_3-N$, $NH_3-N$, TP, DTP, $PO_4-P$, and phytoplankton community. The water quality of the main stream and the tributaries were not significantly different apart from the relatively high concentration of BOD, COD and nutrients recorded in MH. The highest cell density of Stephanodiscus hantzschii and Merismopedia glauca dominated phytoplankton was observed in PD. Based on the correlation analysis, total phytoplankton and cyanophyceae were highly correlated with BOD, COD and nutrients. PCA/FA resulted in four main factors accounting for 82.240 % of the total variance in the water quality dataset. The group of component 1 (TN, DTN, DO, $NO_3-N$, water temperature) and component 2 ($PO_4-P$, T-P, DTP, SS) were classified as nutrient element factor whereas component 3 (Chl-a, COD, BOD, $NH_3-N$, pH) was related to organic substances. Hence, the identification of the main potential environmental pollution factors in Bukhan River will help policy makers make better and more informed decisions on how to improve the water quality.

The Effect of Self-Deterministic Factors of Social Network Service Users on Psychological Well-Being by Self-Esteem and Satisfaction (소셜 네트워크 서비스 이용자의 자기결정성 요인이 자기존중감과 만족을 통해 심리적 안녕감에 미치는 영향)

  • Han, Jeong Seop;Kwon, Do soon;Kim, Seong Jun
    • Journal of Korea Society of Digital Industry and Information Management
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    • v.17 no.1
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    • pp.85-108
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    • 2021
  • In this study, we would like to consider the main factors that influence psychological well-being by using social network service. suggest ways to use healthy social network services, and empirically verify how behavioral approaches, in other words self-determination theory, that deals with human psychological needs, affect psychological well-being by self-esteem and satisfaction to comprehensively examine the characteristics of service and user individuals and social influences, etc. First research result, it has been confirmed that the autonomy has a positive effect on self-esteem. Second, it has been confirmed that the autonomy does not have a positive effect on satisfaction. Third, it has been confirmed that the competence has a positive effect on self-esteem. Fourth, it has been confirmed that the competence does not have a positive effect on satisfaction. Fifth, it has been confirmed that the relationship has a positive effect on self-esteem. Sixth, it has been confirmed that the relationship does not have a positive effect on satisfaction. Seventh, it has been confirmed that self-esteem has a positive effect on psychological well-being. Eighth, it has been confirmed that self-esteem has a positive effect on satisfaction. Ninth, it has been confirmed that satisfaction has a positive effect on psychological well-being. These results mean that meeting basic human psychological needs has a significant impact on psychological well-being of the social network service users. In addition, it was confirmed that increasing the desire for autonomy, competence, relationship, which are factors of self-determination, is an important factor to enhance psychological well-being.

Effects of 3'-isovaleryl-4'-senecioylkhellactone from Peucedanum japonicum Thunberg on PMA-Stimulated Inflammatory Response in A549 Human Lung Epithelial Cells

  • Hwang, Daseul;Ryu, Hyung Won;Park, Ji-Won;Kim, Jung-Hee;Kim, Doo-Young;Oh, Jae-Hoon;Kwon, Ok-Kyoung;Han, Sang-Bae;Ahn, Kyung-Seop
    • Journal of Microbiology and Biotechnology
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    • v.32 no.1
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    • pp.81-90
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    • 2022
  • Peucedanum japonicum Thunberg (PJT) has been used in traditional medicine to treat colds, coughs, fevers, and other inflammatory diseases. The goal of this study was to investigate whether 3'-isovaleryl-4'-senecioylkhellactone (IVSK) from PJT has anti-inflammatory effects on lung epithelial cells. The anti-inflammatory effects of IVSK were evaluated using phorbol 12-myristate 13-acetate (PMA)-stimulated A549 cells and regular human lung epithelial cells as a reference. IVSK reduced the secretion of the inflammatory mediators interleukin (IL)-8 and monocyte chemoattractant protein-1 (MCP-1), and the mRNA expression of IL-6, IL-8, MCP-1, and IL-1β. Additionally, it inhibited the phosphorylation of IκB kinase (IKK), p65, Iκ-Bα, and mitogen-activated protein kinases (MAPKs) p38, JNK, and ERK in A549 cells stimulated with PMA. Moreover, the binding affinity of activator protein-1 (AP-1) and nuclear factor-κB (NF-κB) was significantly reduced in the luciferase assay, while nuclear translocation was markedly inhibited by IVSK in the immunocytochemistry. These findings indicate that IVSK can protect against inflammation through the AP-1 and NF-κB pathway and could possibly be used as a lead compound for the treatment of inflammatory lung diseases.

Environment-Friendly Control of Cucumber Downy Mildew Using Chlorine Dioxide (이산화염소수를 활용한 오이 노균병 친환경방제)

  • Kim, Byung-Ryun;Hahm, Soo-Sang;Kwon, Mi-Kyung;Kim, Yun-Jeong;Kim, Woon-Seop;Song, Jeong-Young;Oh, Sang-Keun;Ju, Jung-Il
    • Research in Plant Disease
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    • v.27 no.4
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    • pp.149-154
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    • 2021
  • Pseudoperonospora cubensis (downy mildew) is highly virulent to various Cucurbitaceae crops, including cucumber (Cucumis sativus). We tested chlorine dioxide application in a plastic greenhouse for environment-friendly control of downy mildew disease. Spraying diluted chlorine dioxide suppressed downy mildew disease with 41.2% control efficacy. Thermal fogging with chlorine dioxide had a high control efficacy of 80.9%, confirming that this approach is useful for environment-friendly downy mildew control. Using thermal fogging to control diseases that are greatly affected by humidity, such as downy mildew, may be more effective compared with conventional dilution spray control methods.

Preparation and Gas Permeation Characteristics of Polyetherimide Hollow Fiber Membrane for the Application of Hydrogen Separation (수소분리를 위한 Polyetherimide계 고분자 중공사막의 제조 및 기체투과 특성)

  • Kwon, Hyeon Woong;Im, Kwang Seop;Kim, Ji Hyeon;Kim, Seong Heon;Kim, Do Hyeong;Nam, Sang Yong
    • Membrane Journal
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    • v.31 no.6
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    • pp.456-470
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    • 2021
  • In this study, polyetherimide-based hollow fiber membranes were manufactured using the NIPS (nonsolvent induced phase separation) method. THF, Ethanol, and LiNO3 were used as additives to control the morphology of the PEI-hollow fiber membranes. Furthermore, for the development of a high hydrogen separation membrane, the spinning conditions were optimized through the characterization of SEM and gas permeance. As a result, as the content of THF increased, the hydrogen/carbon dioxide selectivity increased. However, the permeance decreased due to the trade-off relationship. When ethanol was added, a finger-like structure was shown, and when LiNO3 was added, a sponge structure was shown. In particular, in the case of a hollow fiber membrane with an optimized PDMS coating layer, the permeance was 40 GPU and the hydrogen/carbon dioxide selectivity was 5.6.

The effect of temperature and breeding density of piggery on the collection of oral fluid in Korea (국내 양돈장의 사육 온도와 밀사율이 구강액 채취율에 미치는 영향)

  • Byeon, Hyeon Seop;Kim, Mihwa;Kwon, Sungae;Han, Mina;Han, Sung Tae;Jang, Rae Hoon;Chung, Yun-Soo;Kim, Seokhyun;Jeon, Bo-Young
    • Korean Journal of Veterinary Service
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    • v.44 no.4
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    • pp.217-225
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    • 2021
  • We investigated the effect of temperature and stock density on the collection efficiency of oral fluid in the pig farm in Korea. Three pig farms with similar breeding environmental conditions were selected and four pens of each farm (total 12 pens) were tested for the collection efficiency of oral fluid from pigs. Collection rate was considered as significant when oral fluid was collected from 70% of pigs within a pen. In the case of growing pigs, when internal temperature of pig barn increased by one designated degree (5℃), the oral fluid collection rate significantly decreased by 24.7% (P<0.05). The collection rate of oral fluid also decreased by 7.1% (P<0.05) as the density rate increase by one designated degree (12.5%). It was estimated that the collection efficiency of oral fluid decreased when the internal temperature of pig barn was 30℃ or higher, or barn density is higher 25% or high. On the other hand, in the case of stall-housing sows, unlike growing pigs, there was no significant differences according to the temperature, so oral fluid collection was considered to be efficient even in hot season.