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Growth Survival of Listeria monocytogenes in Enoki Mushroom (Flammulina velutipes) at Different Temperatures and Antilisterial Effect of Organic Acids (팽이버섯에서 Listeria monocytogenes의 온도별 생존과 유기산에 의한 저감화)

  • Kim, Se-Ri;Kim, Won-Il;Yoon, Jae-Hyun;Jeong, Do-Yong;Choi, Song-Yi;Hwang, Injun;Rajalingam, Nagendran
    • Journal of Food Hygiene and Safety
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    • v.35 no.6
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    • pp.630-636
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    • 2020
  • Listeria monocytogenes (L. monocytogenes) was responsible for several recall cases owing to its incidence in mushrooms exported from the Republic of Korea. In this study, we investigated the survival of L. monocytogenes in enoki mushroom (Flammulina velutipes) at different temperatures and the antilisterial effect of its organic acids. Enoki mushrooms were innoculated with L. monocytogenes (initial concentration 4.5 log CFU/g) and stored at 1-35℃, No growth of L. monocytogenes in enoki mushrooms was observed at 1℃ for 30 days. 3.0 log CFU/g growth of L. monocytogenes was also achieved after 36 h and 24 h at 30℃ and 35℃, respectively. To evaluate the antilisterial effect of the organic acids (acetic acid, lactic acid, malic acid), enoki mushrooms were treated with 1-3% of each acid for 10-30 min. The efficacy of malic acid and lactic acid was significantly higher than that of acetic acid. Over 3.0 log reductions were observed when L. monocytogenes in enoki mushrooms was immersed in 3% lactic acid and malic acid over 10 minutes or more. Therefore, it is necessary to keep enoki mushrooms at 1℃ during the export process and treat them with 3% lactic acid and malic acid for 10 min prior to consumption.

Analysis of Microclimate Impact According to Development Scenarios of Vacant Land in Downtown Seoul - A Comparison of Wind Speed and Air Temperature - (서울 도심 공지의 개발 시나리오에 따른 미기후 영향 분석 - 풍속 및 기온 비교 -)

  • Baek, Jiwon;Park, Chan;Park, Somin;Choi, Jaeyeon;Song, Wonkyong;Kang, Dain;Kim, Suryeon
    • Journal of Environmental Impact Assessment
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    • v.30 no.2
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    • pp.105-116
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    • 2021
  • In the city of high population density crowded with buildings, Urban Heat Island (UHI) is intensified, and the city is vulnerable to thermal comfort. The maintenance of vacant land in downtown is treated as a factor that undermines the residential environment, spoils the urban landscape, and decreases the economic vitality of the whole region. Therefore, this study compared the effects on microclimate in the surrounding area according to the development scenarios targeting the vacant land in Songhyeon-dong, Jongno-gu, Seoul. The status quo, green oriented, building oriented and green-building mediation scenarios were established and ENVI-met was used to compare and analyze the impact of changes in wind speed, air temperature and mean radiant temperature (MRT) within 1 km of the target and the target site. The result of inside and 1 km radius the targeted area showed that the seasonal average temperature decreased and the wind speed increased when the green oriented scenario was compared with the current state one. It was expected that the temperature lowered to -0.73 ℃ or increased to 1.5 ℃ in summer, and the wind speed was affected up to 210 meters depending on the scenario. And it was revealed that green area inside the site generally affects inside area, but the layout and size of the buildings affect either internal and external area. This study is expected to help as a decision-making support tool for developing Songhyeon-dong area and to be used to reflect the part related to microclimate on the future environmental effects evaluation system.

Effect of Cardanol Content on the Antibacterial Films Derived from Alginate-PVA Blended Matrix (알지네이트-폴리비닐알콜 블랜드 항균 필름 제조를 위한 카다놀 함량의 영향)

  • Ahn, Hee Ju;Kang, Kyung Soo;Song, Yun Ha;Lee, Da Hae;Kim, Mun Ho;Lee, Jae Kyoung;Woo, Hee Chul
    • Clean Technology
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    • v.28 no.1
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    • pp.24-31
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    • 2022
  • Petroleum-based plastics are used for various purposes and pose a significant threat to the earth's environment and ecosystem. Many efforts have been taken globally in different areas to find alternatives. As part of these efforts, this study manufactured alginate-based polyvinyl alcohol (PVA) blended films by casting from an aqueous solution prepared by mixing 10 wt% petroleum-based PVA with biodegradable, marine biomass-derived alginate. Glutaraldehyde was used as a cross-linking agent, and cardanol, an alkyl phenol-based bio-oil extracted from cashew nut shell, was added in the range of 0.1 to 2.0 wt% to grant antibacterial activity to the films. FTIR and TGA were performed to characterize the manufactured blended films, and the tensile strength, degree of swelling, and antibacterial activity were measured. Results obtained from the FTIR, TGA, and tensile strength test showed that alginate, the main component, was well distributed in the PVA by forming a matrix phase. The brittleness of alginate, a known weakness as a single component, and the low thermal durability of PVA were improved by cross-linking and hydrogen bonding of the functional groups between alginate and PVA. Addition of cardanol to the alginate-based PVA blend significantly improved the antibacterial activity against S. aureus and E. coli. The antibacterial performance was excellent with a death rate of 98% or higher for S. aureus and about 70% for E. coli at a contact time of 60 minutes. The optimal antibacterial activity of the alginate-PVA blended films was found with a cardanol content range between 0.1 to 0.5 wt%. These results show that cardanol-containing alginate-PVA blended films are suitable for use as various antibacterial materials, including as food packaging.

Beneficial constituents and physiological activity of fermented Codonopsis lanceolata and Platycodon grandiflorus by Lentinula edodes mycelium (더덕, 도라지 표고균사발효물의 유용성분 및 생리활성)

  • Koh, Young-Woo;Yun, Kyeong-Won;Kim, Kyung-Je;Jin, Seong-Woo;Im, Seung-Bin;Ha, Neul-I;Jeong, Hee-Gyeong;Kim, Seung-Ju;Kim, Bok-Seon;Choi, Yu-Jin;Song, Da-Hye;Seo, Kyoung-Sun
    • Journal of Mushroom
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    • v.19 no.4
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    • pp.300-309
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    • 2021
  • This study was conducted to develop food and medicinal products containing useful components of Lentinula edodes in Codonopsis lanceolata and Platycodon grandiflorus for use as herbal medicine. We manufactured C. lanceolata (FCLM) and P. grandiflorus (FPLM) extract fermented with L. edodes mycelium. The effect of the two fermented products on proximate composition, free sugar, organic acid, 𝛽-glucan, ergothioneine, ergosterol, and vitamin D2 levels, and 3T3-L1 preadipocyte cell growth were studied. The proximate composition analysis results showed that the crude fiber and crude fat content in FCLM was higher than that in FPLM, and the crude protein and soluble nitrogen content in FPLM was higher than that in FCLM. Free sugar analysis detected arabinose, glucose, and sucrose in both FCLM and FPLM, and the total free sugar content was high in FPLM. The organic acid content was lower in FCLM and FPLM compared to C. lanceolata and P. grandiflorus before fermentation. The 𝛽-glucan content was higher than that of L. edodes used as a control in both fermented products, FCLM and FPLM. The content of ergothioneine, an antioxidant, was higher in FCLM than in FPLM. Ergosterol content was highest in L. edodes which was used as a control, and the two fermented products showed similar content. Vitamin D2 was detected only in FCLM and FPLM, and FPLM (0.58±0.01 mg%) showed a higher vitamin D2 content than FCLM (0.47±0.01). FCLM and FPLM showed a higher level of cell viability for 3T3-L1 pre-adipocytes compared to non-fermented C. lanceolata and P. grandiflorus. In addition, FCLM and FPLM inhibited 3T3-L1 preadipocyte differentiation more than C. lanceolata and P. grandiflorus before fermentation, which may exert an anti-obesity effect.

The Effects of the High-tech Manufacturing Ventures' External Collaborations on the Management Performance: Focusing on the Mediation Effect of Internal Core Competencies (첨단제조 벤처기업의 외부적 협력활동 경험이 경영성과에 미치는 영향에 관한 연구: 내부 핵심역량의 매개효과를 중심으로)

  • Lee, Younghun;Song, Eugene
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.16 no.6
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    • pp.69-84
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    • 2021
  • As industrial structural changes in the 4th Industrial Revolution have recently led to the need for fostering high-tech industries and high-tech manufacturing industries have been showing high value-added creation, the importance of high-tech manufacturing ventures has increased a lot as well. As a result of this, the government is actively supporting and fostering them. However, it appears that high-tech manufacturing ventures seem to have a lot of difficulty in securing competitive advantages due to the lack of internal core competencies and experience in the rapidly changing international economic conditions. In order for high-tech manufacturing ventures to strengthen internal core competencies, external collaborations with other companies or institutions which have diverse experience, technology skills and abundant resources are actively promoted. Accordingly, based on resource-based theory and transaction cost theory, the authors analyzed the effects of the high-tech manufacturing ventures'external collaborations on internal core competencies and management performance in this study. In order to verify the hypothesis of this study, the 2020 data on"The Research on the Precision Status of Ventures'compiled by the Ministry of SMEs and Startups since 1999 were utilized. According to the results of this study, the experience of external collaborations had a positive impact on the internal core competencies and non-financial management performance, while there was no direct impact on financial management performance. Moreover, the relationship between the experience of external collaborations and management performance is mediated by the internal core competencies. Additionally, it was found that the internal core competencies positively affected both non-financial and financial management performances, and non-financial management performance again had a significant impact on the financial management performance. Finally, the experience of external collaborations had a positive impact on both development, manufacturing, and marketing factors forming the internal core competencies. However, the impacts of individual factors were different in the management performance. Development and marketing factors were shown to have a significant impact on both non-financial and financial management performance, while the manufacturing factor had a significant impact only on financial management performance.

Analysis of Rice Blast Outbreaks in Korea through Text Mining (텍스트 마이닝을 통한 우리나라의 벼 도열병 발생 개황 분석)

  • Song, Sungmin;Chung, Hyunjung;Kim, Kwang-Hyung;Kim, Ki-Tae
    • Research in Plant Disease
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    • v.28 no.3
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    • pp.113-121
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    • 2022
  • Rice blast is a major plant disease that occurs worldwide and significantly reduces rice yields. Rice blast disease occurs periodically in Korea, causing significant socio-economic damage due to the unique status of rice as a major staple crop. A disease outbreak prediction system is required for preventing rice blast disease. Epidemiological investigations of disease outbreaks can aid in decision-making for plant disease management. Currently, plant disease prediction and epidemiological investigations are mainly based on quantitatively measurable, structured data such as crop growth and damage, weather, and other environmental factors. On the other hand, text data related to the occurrence of plant diseases are accumulated along with the structured data. However, epidemiological investigations using these unstructured data have not been conducted. The useful information extracted using unstructured data can be used for more effective plant disease management. This study analyzed news articles related to the rice blast disease through text mining to investigate the years and provinces where rice blast disease occurred most in Korea. Moreover, the average temperature, total precipitation, sunshine hours, and supplied rice varieties in the regions were also analyzed. Through these data, it was estimated that the primary causes of the nationwide outbreak in 2020 and the major outbreak in Jeonbuk region in 2021 were meteorological factors. These results obtained through text mining can be combined with deep learning technology to be used as a tool to investigate the epidemiology of rice blast disease in the future.

Novel Role of Dipterocarpus tuberculatus Roxb. as a Lipogenesis Inhibitor and Lipolysis Stimulator in 3T3-L1 Adipocytes (3T3-L1 지방세포에서 lipogenesis 저해제와 lipolysis 촉진제로서 Dipterocarpus tuberculatus Roxb.의 새로운 역할)

  • Su Jin, Lee;Ji Eun, Kim;Yun Ju, Choi;You Jeong, Jin;Yu Jeong, Roh;AYun, Seol;Hee Jin, Song;Dae Youn, Hwang
    • Journal of Life Science
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    • v.32 no.11
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    • pp.855-864
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    • 2022
  • The pharmacological efficacy of Dipterocarpus tuberculatus Roxb. has been verified in only several fields including photoaging, inflammation, hepatotoxicity, acute gastritis and osseointegration. To identify the novel functions of Dipterocarpus tuberculatus Roxb. on anti-obesity, inhibitory effect on lipid accumulation and stimulatory effect on lipolysis were investigated in MDI (3-isobutyl-1-methyl-xanthine, dexamethasone, and insulin) stimulated 3T3-L1 adipocytes treated with methanol extracts of Dipterocarpus tuberculatus Roxb. (MED). Lipogenic targets, including lipid accumulation, level of lipogenic transcription factors, and expression of lipogenic regulators, were downregulated in MDI-stimulated 3T3-L1 adipocytes treated with MED without any significant cytotoxicity. Also, MED treatment inhibited the mRNA levels of adipogenic targets including peroxisome proliferator-activated receptor (PPAR)γ and CCAAT-enhancer binding protein (C/EBP) α, as well as lipogeic targets including adipocyte fatty acid binding protein 2 (aP2) and fatty acid synthetase (FAS) in MDI-stimulated 3T3-L1 adipocytes. A similar decrease patterns were detected in Oil red O stained lipid droplets of MED treated MDI-stimulated 3T3-L1 adipocytes. Furthermore, several lipolytic targets, such as cAMP concentration, concentration of free glycerol, expression level of lipases, including ATGL, perilipin and HSL, were upregulated in MDI-stimulated 3T3-L1 adipocytes treated with MED. These results show that MED has a novel role as a lipogenesis inhibitor and lipolysis stimulator in MDI-stimulated 3T3-L1 adipocytes.

Analysis of Pesticide Residues in Stalk and Stem Vegetables Marketed in Northern Gyeonggi-do (경기 북부 지역 유통 엽경채류의 농약 잔류량 분석)

  • Yoo, Na-Young;Kim, Ki-Yu;Kim, Yun-Sung;Kim, Sang-Tae;Song, Seo-Hyeon;Lim, Jeong-Hwa;Han, Yoo-Li;Choi, Hee-Jeong;Kim, Youn-Ho;Seo, Jeong-Hwa;Choi, Ok-Kyung
    • Journal of Food Hygiene and Safety
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    • v.37 no.3
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    • pp.149-158
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    • 2022
  • This study aimed to investigate pesticide residues in 160 stalk and stem vegetables marketed in Northern Gyeonggi-do. The QuEChERS method using GC-MS/MS and LC-MS/MS was employed to analyze the residues of 341 pesticides in the samples. The maximum or lower than the residue limit was recorded in 75 samples (46.9%), while 4 samples (2.5%) exceeded the maximum residue limit (MRL). Thirty-nine kinds of residual pesticides were detected including fungicides (14), insecticides (22), herbicides (2), and plant growth regulator (1). Carbendazim and pendimethalin were the most frequelntly detected pesticides. Fenitrothion, procymidone, and diazinon exceeded MRL in garlic chives, and Welsh onion. This indicated that these vegetables along with water celery should be constantly monitored.

Applicability Analysis of the HSPF Model for the Management of Total Pollution Load Control at Tributaries (지류총량관리를 위한 HSPF 모형의 적용성 분석)

  • Song, Chul Min;Kim, Jung Soo;Lee, Min Sung;Kim, Seo Jun;Shin, Hyung Seob
    • Ecology and Resilient Infrastructure
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    • v.9 no.1
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    • pp.1-14
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    • 2022
  • The total maximum daily load (TMDL) implemented in Korea mainly manages the mainstream considering a single common pollutant and river discharge, and the river system is divided into unit watersheds. Changes in the water quality of managed rivers owing to the water quality management in tributaries and unit watersheds are not considered when implementing the TMDL. In addition, it is difficult to consider the difference in the load of pollutants generated in the tributary depending on the conditions of the water quality change in each unit watershed, even if the target water quality was maintained in the managed water system. Therefore, it is necessary to introduce the total maximum load management at tributaries to manage the pollution load of tributaries with a high degree of pollution. In this study, the HSPF model, a watershed runoff model, was applied to the target areas consisting of 53 sub-watersheds to analyze the effect of water quality changes the in tributaries on the mainstream. Sub-watersheds were selected from the three major areas of the Paldang water system, including the drainage basins of the downstream of the South Han-River, Gyeongan stream, and North Han-River. As a result, BOD ranged from 0.17 mg/L to 4.30 mg/L, and was generally high in tributaries and decreased in the downstream watershed. TP ranged from 0.02 mg/L - 0.22 mg/L, and the watersheds that had a large impact on urbanization and livestock industry were high, and the North Han-River basin was generally low. In addition, a pollution source reduction scenario was selected to analyze the change in water quality by the amount of pollution load discharged at each unit watershed. The reduction rate of BOD and TP according to the scenario changes was simulated higher in the watershed of the downstream of the North Han-River and downstream and midstream of the Gyeongan stream. It was found that the benefits of water quality reduction from each sub-watershed efforts to improve water quality are greatest in the middle and downstream of each main stream, and it is judged that it can be served as basic data for the management of total tributaries.

A Study on Risk Assessment Method for Earthquake-Induced Landslides (지진에 의한 산사태 위험도 평가방안에 관한 연구)

  • Seo, Junpyo;Eu, Song;Lee, Kihwan;Lee, Changwoo;Woo, Choongshik
    • Journal of the Society of Disaster Information
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    • v.17 no.4
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    • pp.694-709
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    • 2021
  • Purpose: In this study, earthquake-induced landslide risk assessment was conducted to provide basic data for efficient and preemptive damage prevention by selecting the erosion control work before the earthquake and the prediction and restoration priorities of the damaged area after the earthquake. Method: The study analyzed the previous studies abroad to examine the evaluation methodology and to derive the evaluation factors, and examine the utilization of the landslide hazard map currently used in Korea. In addition, the earthquake-induced landslide hazard map was also established on a pilot basis based on the fault zone and epicenter of Pohang using seismic attenuation. Result: The earthquake-induced landslide risk assessment study showed that China ranked 44%, Italy 16%, the U.S. 15%, Japan 10%, and Taiwan 8%. As for the evaluation method, the statistical model was the most common at 59%, and the physical model was found at 23%. The factors frequently used in the statistical model were altitude, distance from the fault, gradient, slope aspect, country rock, and topographic curvature. Since Korea's landslide hazard map reflects topography, geology, and forest floor conditions, it has been shown that it is reasonable to evaluate the risk of earthquake-induced landslides using it. As a result of evaluating the risk of landslides based on the fault zone and epicenter in the Pohang area, the risk grade was changed to reflect the impact of the earthquake. Conclusion: It is effective to use the landslide hazard map to evaluate the risk of earthquake-induced landslides at the regional scale. The risk map based on the fault zone is effective when used in the selection of a target site for preventive erosion control work to prevent damage from earthquake-induced landslides. In addition, the risk map based on the epicenter can be used for efficient follow-up management in order to prioritize damage prevention measures, such as to investigate the current status of landslide damage after an earthquake, or to restore the damaged area.