• Korean Journal of Environmental Biology
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• v.34 no.3
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• pp.212-215
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• 2016

We developed a new ecotoxicological test method using native test species, eggs of Ephemera orientalis, and five kinds of industrial wastewater were tested to validate the test method. The water samples were collected in Jun 2006 from the following industries: pesticide, metal plating, PCB, leather1, and leather2. Wastewater and effluent were diluted by distilled water, respectively, to prepare various concentrations, 100, 50, 25, 12.5, 6.3, 3.1, and 0%. For the egg bioassay, 20 freshly laid eggs (<24 h old) were exposed to test solutions in a Petri dish ($52{\times}12mm$) at $20^{\circ}C$ with photoperiod of 16 h light and 8 h dark for 14 days. The median egg hatching concentrations (EHC50) were estimated using Probit analysis. All EHC50s of wastewater were less than 3.1%, which meant very high ecotoxicity except for the wastewater of PCB industry having 6.1% of EHC50. Among the effluents, the least toxic effluent was from pesticide industry having 58% of EHC50, while the effluent of leather2 was the most toxic having 7.3% of EHC50.

• Korean Journal of Plant Resources
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• v.19 no.1
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• pp.76-82
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• 2006

Genetic variation of two Juniperus chinensis var. sargentii populations in Mt. Seorak and Mt. Halla was investigated by isozyme analysis at reproducible 11 loci of 7 isozyme systems(Aat-1, Aat-2, Gdh, Idh, Lap, Mdh-1, Mdh-2, Mdh-3, 6Pgd, Pgi-1, and Pgi-2), of which 7 loci were polymorphic. The levels of genetic diversity of two populations were A=2.2, $A_e=1.61,\;P_{95}=54.5,\;H_{o}=0.179,\;H_e=0.287$(Mt. Seorak population) and A=2.1, $A_e=1.48,\;P_{95}=63.6,\;H_{o}=0.270,\;H_e=0.250$(Mt. Halla population), respectively. These values were similar to and/or somewhat higher than those observed in other Korean native conifers. Moderately low degree of genetic differentiation was observed between 2 analyzed populations ($F_{ST}=0.039$). Heterozygosity of the population in Mt. Seorak was significantly lower than expected, and much high level of inbreeding coefficient(F=0.376) was observed. Considering the limited population size and distribution range of the population, the population seemed to be influenced by inbreeding and/or random genetic drift, Consequently, Mt. Seorak population should be considered to be a more important candidate for the conservation of J. chinensis var. sargentii.

• Korean Journal of Medicinal Crop Science
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• v.16 no.6
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• pp.434-438
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• 2008

To selection of optimal shading material, two-year-old ginseng (Panax ginseng C. A. Meyer) of new cultivar, 'Cheonpoong' (CP), and native species 'Hwangsookjong' (HS) were cultured under three kinds of shading materials such as three-layered blue and a one-layered black PE (polyethylene) net (TBSB), blue PE sheet (BS), and aluminium coated PE sheet (AS) in imperfectly drained paddy soil. Growth characteristics, yield and ginsenoside contents were investigated under three shading materials. Yield and ginsenoside contents of ginseng were distinctly affected by intensity and quality of sunlight penetrated through shading materials. Light transmission ratio, air and soil temperature according to shading materials were higher in order of BS, AS, and TBSB. However, ratio of aerial phase and porosity of the soil were higher in order of AS, BS, and TBSB, respectively. There was no significantly difference in the ratio of rusty colored root by shading materials. CP showed higher stem length, leaf area, and root weight than that of HS, while the former showed distinctly lower discolored leaf ratio than that the other. Eight kinds of ginsenosides content of CP were higher than that of HS in $Rg_1$, Re, Rf, $Rb_1$ and Rc except $Rg_2$, $Rb_2$, and $Rb_3$. Total ginsenoside contents of CP was distinctly higher than that of HS. Total ginsenoside contents as affected by shading materials was higher in order of BS, TBSB, and AS in CP, while TBSB, BS, and AS in HS.

• Korean Journal of Plant Resources
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• v.20 no.4
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• pp.367-374
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• 2007

This study was carried out to establish the micropropagation system of Bupleurum latissimum Nakai that is a Korean native endangered species. Callus were induced from the leaf, petiole and floral bud and the percentage of callus formation was highest in the floral bud on the MS medium containing 2.0 mg ${\cdot}$ $L^{-1}$ 2,4-D. Especially, callus induced from floral bud was formed 77.8% and the percentage of shoot formation was 42.6% on the MS medium containing 2.0 mg ${\cdot}$ $L^{-1}$ 2,4-D plus 1.0 mg ${\cdot}$ $L^{-1}$ TDZ. For simultaneously callus formation and shoot regeneration, 1/2 MS medium was more effective than MS medium. The percentage callus formation, shoot regeneration and rooting were 46.3%, 13.0%, 13.0% in 1/2 MS medium, respectively. Soot regeneration from callus was good in 1/2 MS medium supplemented with 2.0 mg ${\cdot}$ $L^{-1}$ 2,4-D plus 1.0 mg ${\cdot}$ $L^{-1}$ BA where percentage of shoot regeneration was 74.1 %, and the number of shoot per explant was 2.4. The percentage of rooting was lowest (57.8%) in control while it was highest (97.8%) in 1.5 mg ${\cdot}$ $L^{-1}$ NAA. In acclimatization of regenerated plantlets, the percentage of survived plantlets was highest (86.1%), and plant height, root length and fresh weight were good in the soil for horticulture.

• Korean Journal of Agricultural Science
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• v.38 no.4
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• pp.631-639
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• 2011

This research was conducted to investigate potential use of New Zealand spinach (Tetragonia tetragonoides) as a new vegetable crop which will be cultivating in salt-affected soils including reclaimed land. Traditionally New Zealand spinach has been studied to explore functional compound or salt removing potential. To cultivate the crop species in the salt-affected soil widely, it is essential to obtain salt and soil texture responses under the controlled environment. Fifty nine New Zealand spinach ecotypes native to Korean peninsula first collected over seashore areas, and primitive habitat soil environment was evaluated by analyzing soil chemical properties from 32 locations. Different textures of sandy, silt loam, and sandy loam soils were prepared from nearby sources of sea shore, upland and paddy soils, respectively. Target salinity levels of 16.0 dS/m, 27.5 dS/m, 39.9 dS/m, and 52.4 dS/m in electrical conductivity (ECw) were achieved by diluting of 25, 50, 75, 100% (v/v) sea water to tap water (control, 0.6 dS/m), respectively. Various measurements responding to soil texture and irrigation salinity included plant height, root length, fresh weight (FW), dry weight (DW), leaf parameters (leaf number, leaf length, leaf width), lateral branching, and inorganic ion content. was found to adapt to diverse habitats ranging various soil chemical properties including soil pH, organic matter, exchangeable bases, EC, and cation exchange capacity (CEC) in Korea. Responding to soil texture, New Zealand spinach grew better in silt loam and sandy loam soil than in sandy soil. Higher yield (FW and DW) seemed to be associated with branch number (r=0.99 and 0.99, respectively), followed by plant height (r=0.94 and 0.97, respectively) and leaf number (r=0.89 and 0.84, respectively). Plant height, FW, and DW of the New Zealand spinach accessions were decreased with increasing irrigation salinity, while root length was not significantly different compared to control. Based on previous report, more narrow spectrum of salinity range (up to 16 dS/m) needs to be further studied in order to obtain more accurate salinity responses of the plant. As expected, leaf Na content was increased significantly with increasing salinity, while K and Ca contents decreased. Growth responses to soil texture and irrigation salinity implied the potential use of New Zealand spinach as a leafy vegetable in salt-affected soil constructed with silt loam or sandy loam soils.

• The Korean Journal of Zoology
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• v.24 no.3
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• pp.151-161
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• 1981

The field studies on the seasonal population prevalences of the vector mosquito larvae and other aquatic invertebrates were weekly carried out in the rice fields located in front of a village of Kwangtan-samri, Byeogje-myeon, Goyang-gun, Gyeonggido throughout mosquito breeding season (June-September) in 1980, and the results are as follows. The population of C. tritaeniorhynchus larvae increased soon after heavy rainfall, and decreased to some extent during the period of the insecticide application. The seasonal prevalence of A. sinensis was rather stable, not being affected by insecticide pressure at all. The population densities of other aquatic invertebrates in rice fields were seriously suppressed by the pesticide application, and their recovery was not as same as the previous level or not at all: (1) The populations of Odonata and Ephemeroptera nymphs were drastically decreased by the first application of insecticides and never recovered through out season. (2) Coleoptera seemed very susceptible to the insecticide application, as densities were markedly decreased whenever the insecticides were applied. (3) The high density of Hemiptera shown in early June were decreased sharply after the first application of pesticides, and thereasfter, some degree of recovery was shown, but suppressed by successive each application of pesticides. (4) A native species of planaria was exceptionally not influenced by the insecticide pressure, and two peaks of the density were appeared during the heavy rainfall.

• Korean Journal of Environmental Agriculture
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• v.16 no.4
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• pp.347-355
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• 1997

For the selection of plants contained pesticidal active conpounds, 31 families 59 species of native plants were collected and biological activites of their crude extracts against brown planthopper (Nilaparvata lugens), Xanthomonas oryzae pv. oryzae and allelopathy were examined. Among the screened plants, the crude extracts from the leaves of Ricinus communis and Sophora angustifolia showed 100% and 82% of mortality on brown planthopper at the concentration of 1% (w/v) respectively. Mixed crude extracts of Sophora angustifolia root and Melia azedarach seed exhibited 128${\sim}$155% of synergistic effects on the mortality of brown planthopper. In case of fungicidal activity, the crude extracts from the leaves of 8 plants including Chrysanthemum indicum were inhibitory of the growth of Xanthomonas oryzae pv. oryzae at 1%(w/v) soluction. Seed germination of radish (Raphanus sativas) and barnyardgrass (Echinochloa crus-galli) was inhibited by 0.5%(w/v) leaf extracts of Pinus densiflora and Quercus acutissima. MeOH extracts of Pinus denislora leaves gave 100% inhibition in seed germintion of radish at 2%(w/v) soluction and showed a complete inhibition in seedling growth of barnyardgrass as well as radish at 5% solution.

• Journal of the Korean Institute of Landscape Architecture
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• v.43 no.2
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• pp.23-29
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• 2015

To fulfill the need for reuse indigenous tree to mitigate the elimination of nature forests due to road construction, one representative method for this reuse is to transplant them and re-establish in similar conditions. In order to investigate the transplant and establishment of indigenous tree, a correlation and regression analysis was conducted by species and tree size. Data were collected for 6 years(2008~2013) in 7 construction sites in cooperation with the Korea Expressway Corporation. Regarding the transplanted indigenous trees status, the success rate of transplant was 15,519(69%) of 22,521. The tree most transplanted was Pinus densiflora(15,562), followed by Quercus spp.(6,156), Prunus sargentii(235), and P. thunbergii(154). P. densiflora and P. thunbergii belong to the conifer group while Quercus spp., Prunus sargentii belong to the broadleaf group. As a result of a contrast test, the conifer group had a significantly lower success rate of transplant than the broadleaf group. In the relation of root collar diameter and success rate of transplant, there was the tendency that the larger the root collar diameter, the lower the success rate of transplant. This study demonstrated that there is a strong negative correlation between the two factors(r=-0.730, p>0.000). The predicted regression equation of the success rate of transplant was Y= -0.811X+88.627(X: root collar diameter, Y: success rate of transplant) and the $R^2$ value for the linear equation was 0.532.

• Korean Journal of Pharmacognosy
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• v.46 no.2
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• pp.148-153
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• 2015

Allium hookeri is a plant species native to India, Sri Lanka, Myanmar, and China. The plant is widely cultivated in Korea lately as a medicinal food item. This study was conducted to evaluate the effects of Allium hookeri (A. hookeri) on lipid metabolism in Type II diabetic mice (n=8/group, 5 groups). High fat diets with dextrin as a positive control (Dex), leaf (AL), root (AR), and fermented root (FAR) at 3% of diet were fed to all experimental mouse, respectively for 8 weeks. Body weight gain, liver and epididymal fat weights, and excreted fecal lipid levels were measured. Serum and hepatic lipid profiles were analyzed, and fat accumulation in liver was evaluated. In this study, body weight gain and epididymal fat weight were lower in the FAR group, while serum HDL-cholesterol level and excreted fecal total lipid and triglyceride levels were higher in AL or FAR groups. These results suggest that A. hookeri, specially fermented root can be a useful food item to control lipid metabolism in diabetic mice.

• Korean Journal of Organic Agriculture
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• v.23 no.3
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• pp.481-495
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• 2015

The study researched germination of the plants and growth of Fungus according to concentration of aqueous extracts in order to provide basic data for developing natural agricultural resources by using Persicaria longiseta. The seed germination of Amaranthus spinosus was inhibited at 25% P. longiseta extract, while Agrostis stolonifera ssp. palustris was not affected at all concentrations tested. Especially, the seed germination rate and fresh weight of Trifolium incarnatum at 20% P. blumei extract were higher than those of control plot. The early growth of most receptor plant seedlings was promoted at 25% and 50% of P. blumei extracts, but the radicle growth of all receptor plants was significantly inhibited at > 25% of P. longiseta extract. The response of receptor plants to P. longiseta extract was different according to the plant species and the plant parts. The growth of plant pathogenic fungus in PDA medium showed an increasing inhibition tendency with increasing concentrations of P. longiseta extract. Especially, P. longiseta extract showed the greatest antimicrobial activity against Phytophthora infestans, Phythium graminicola, and Pythium venterpoolii. The content of total phenolic compound in P. longiseta was higher in leaves (1082.3 mg/L) but lower in roots (228.6 mg/L) and stems (207.8 mg/L), which is an allelopathic chemical. As these results are summarized, P. longiseta have competitive advantage because they release phenolic compounds with allelopathic effect and affect on germination, growth and fungi growth on underground flora compared to native plants and they have eligibility for natural herbicide and germicide.