• Journal of Plant Biotechnology
• /
• v.41 no.3
• /
• pp.134-139
• /
• 2014

The aim of this study was to establish the condition of regeneration for white balloon flower (Platycodon grandiflorum DC. cv. Jangback) and to manage for the raising of seedling with in vitro regenerated plants. It was examined that 0.5 mg/L of NAA and 1.0 mg/L of BA was the best composition for the callus and shoot induction (up to 600%). NAA was better than IBA for the induction of root and it took 16.9 days for the induction of rooting on the MS soild media containing 0.5 mg/L of NAA and the final rooting ratio was up to 75%. Out of 5 different bed soils purchased from local market, "Tosil" was identified to be the best for the acclimation and growth of in vitro regenerated balloon flower. In detail, on 8 weeks after planting of in vitro regenerated plants in pots containing "Tosil" bed soils, the plant hight was increased up to 2-fold (12.8 cm), 3.5-fold (27) for the number of leaf and 1.5-fold (4.5 cm) for the leaf length when compared to the other four bed soils, respectively. Our preliminary results indicate that it is possible to prevent the occurrence of blue balloon flower in the massive cultivated area of white balloon flower by providing the seedlings raised from in vitro regenerated plants.

• Korean Journal of Pharmacognosy
• /
• v.27 no.2
• /
• pp.105-110
• /
• 1996

Antineoplastic activity against human gastric and colon carcinoma cell lines was tested in eighty-three species of Korean plants including Korean medicinal plants which have been frequently used in oriental herb prescriptions. The plant materials were extracted with methanol and the cytotoxic activity was tested using a calorimetric tetrazolium assay (MTT assay). Twenty-six plant extracts against gastric carcinoma cell line, eighteen extracts against colon carcinoma cell line and fourteen plant extracts against both carcinoma cell lines showed antineoplastic activity at the concentration of less than $100{\mu}g/ml$. The effective components from four species have been isolated and reported.

• Korean Journal of Plant Resources
• /
• v.26 no.3
• /
• pp.397-402
• /
• 2013

Morus Folium (Sang-yeop in Korean) is one of the most important Oriental medicinal plants. In Korea, both M. alba and M. cathayana are regarded as the botanical sources for Morus Folium. In order to discriminate M. alba and M. cathayana from their adulterant, M. tricuspidata, mitochondrial NADH dehydrogenase subunit 7 (nad7) intron 2 region was targeted for molecular analysis with universal primers. DNA polymorphisms, including SNP sites, insertions, and deletions, were detected among these three species sequencing data. Based on these DNA polymorphisms, specific primers were designed for the three species respectively. Multiplex PCR was conducted for molecular authentication of M. alba, M. cathayana, and M. tricuspidata with specific primers. The present results indicate that it is possible to identify Morus Folium from its adulterant using mitochondrial nad7 intron 2 region. The established multiplex-PCR system was proved to be effective for identification of Morus Folium. The results indicate that mitochondrial introns can be used for inter-specific polymorphic study, and the described method can be applied for molecular identification of medicinal materials.

• Korean Journal of Medicinal Crop Science
• /
• v.15 no.3
• /
• pp.189-193
• /
• 2007

We investigated the changes of growth characteristics and major components according to harvesting times in Artemisia capillaris. Flower buds farmed on July 30 and flowers were all open on August 30 and seeds were mature in September, As the growth by harvesting time was the best on August 30 so fresh weight and dry weight were the highest. Especially, in this time, plants had no leaves and fresh weight was investigated as 243.7 g composed of 109.6 g capitulum and 134.1 g stem. Scoparone content, a major component, was the highest as 6.50 mg/g DW in capitulum on August 30. Also capillarisin appeared in both leaf and capitulum except stem and capitulum was shown the most capillarisin content as 1.65 mg/g DW on July 30.

• Korean Journal of Pharmacognosy
• /
• v.39 no.2
• /
• pp.155-163
• /
• 2008

This study was carried out to search the natural antioxidants from the 36 medicinal plants. $IC_{50}$ values of linoleic acid peroxidation inhibition to the BHA, BHT and ${\alpha}-tocopherol$ showed $7.2{\mu}g/ml$, $7.3{\mu}g/ml$, and $26.6{\mu}g/ml$, but hexane extract of Zingiber officinale showed $13.2{\mu}g/ml$, EtOAc extracts of Phellinus linteus, Morus alba, Zingiber officinale and butanol extract of Diospyros kaki showed $15.9{\mu}g/ml$, $16.9{\mu}g/ml$, $20.1{\mu}g/ml$, and $21.5{\mu}g/ml$ respectively. $IC_{50}$ values of DPPH radical scavenging activity of BHA, BHT and $\alpha$-tocopherol showed $14.9{\sim}27.7{\mu}g/ml$, but EtOAc extract of Paeonia suffruticosa showed the lowest $6.5{\mu}g/ml$ than those of other extracts, EtOAc extracts of Camellia sinensis, Salvia miltiorrhiz, and Phellinus linteus showed $8.0{\mu}g/ml$, $11.5{\mu}g/ml$, and $13.0{\mu}g/ml$ respectively.

• Korean Journal of Medicinal Crop Science
• /
• v.24 no.3
• /
• pp.237-245
• /
• 2016

Background: Cyanazine is used as a pre-emergent herbicide once during the growing season to control weeds of many upland crops worldwide. This study aimed to establish a method to determined cyanazine residue levels in major medicinal crops by using high performance liquid chromatography-UV detection/mass spectometry (HPLC-UVD/MS). Methods and Results: Cyanazine residue was extracted with acetone from the raw products of four representative medicinal plants - Scutellaria baicalensis, Paeonia lactiflora, Platycodon grandiflorum and Angelica gigas. The extract was diluted with a large volume of saline water and directly partitioned into dichloromethane to remove polar co-extractives in the aqueous phase. It was then purifined using optimized Florisil column chromatography. HPLC analysis conducted using an octadecylsilyl column allowed the successful separation of cyanazine from co-extractives of the samples, and the amount was sensitively quantified by ultraviolet absorption at 225 nm with no interference. The accuracy and precision of the proposed method were validated by conducting recovery experiments on each medicinal crop sample fortified with cyanazine at two concentration levels per crop in triplicate. Conclusions: The mean recoveries ranged from 91.2% to 105.3% for the four representative medicinal crops. The coefficients of variation were less than 10%, irrespective of the sample types and fortification levels. The limit of quantification of cyanazine was 0.02 mg/kg as verified by the recovery experiment. A confirmatory method was performed by liquid chromatography/MS using selected-ion monitoring technique to clearly identify the suspected residue.

• Korean Journal of Plant Resources
• /
• v.22 no.2
• /
• pp.159-179
• /
• 2009

This study was carried out to investigate the distribution of vascular plants and their usefulness in Mt. Ilsan (Hwacheon-gun, Gangwon-do). The vascular plants were summarized as 496 taxa; 91 families, 295 genera, 422 species, 1 subspecies, 58 varieties, and 15 forma. Among the investigated 496 taxa, 17 Korean endemic, nine rare, and one endangered plants were included. Based on the list of the species of specially designated plants by the Ministry of Environment, 66 taxa including Hanabusaya asiatica and Aconitum koreanum were recorded in the investigated area. The naturalized plants were identified as 27 taxa and the percent of naturalized plant species(PN) was 5.4% of all 496 taxa vascular plants. Usage of 496 taxa were consists of 201 taxa(40.5%) of edible plants, 168 taxa(33.9%) of medicinal plants, 69 taxa(13.9%) of pasture plants, 61 taxa(12.3%) of ornamental plants, 17 taxa(3.4%) of timber plants, 16 taxa(3.2%) of fiber plants and 4 taxa(0.8%) of industrial plants.

• Korean Journal of Plant Resources
• /
• v.28 no.1
• /
• pp.79-92
• /
• 2015

This study was conducted during two separate periods, from April to December 2008 and from April to November 2013, to identify the folk plants growing in Jeollanam-do which are used daily. A total of 1,200 questionnaires were distributed to 120 residents living in 14 counties and cities. From the results of the survey, 172 taxa of folk plants (77 families, 153 genera, 154 species, 2 subspecies, 14 varieties, and 2 forma) were identified. By regions, the city of Suncheon yielded 82 taxa, followed by 67 taxa from Gurye county, 65 taxa from Hampyeong county, 64 taxa from Jangseong county, 62 taxa from city of Naju, and 61 taxa from Jangheung county. Uses of the identified folk plants were: as edibles (117 taxa), for medicinal purposes (66 taxa), tea (15 taxa), for starch (6 taxa) for vegetable fat and oil (3 taxa), for nectar (2 taxa), for sap (2 taxa), for spice (2 taxa), for aroma (1 taxa), for fiber (1 taxa), and others (14 taxa). Thus, edible and medicinal uses of the plants appeared higher than other uses. The consistency of the local names of plants with the standard botanical nomenclature was 23.2% (40 taxa), while the proportion of local plant names analogous to the standard names was 18.6% (32 taxa). The consistency of the local plant names with the same plants in different cities and counties was 28.5% (49 taxa) and the proportion of local plant names analogous to each other in this category was 25.6% (44 taxa). Therefore, 54% of the folk plants growing in Jeollanam-do are known by location specific names. The usage types of the local folk plants were mostly collected through verbal communications.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2022.09a
• /
• pp.48-48
• /
• 2022

Ethnobotany is an interdisciplinary science at the intersection of botany and ethnology. Currently, there is a sharply increasing need for the study and conservation of traditional knowledge about plants. The loss of traditional sources, knowledge, and practices in using plants is caused by the growth of technologies in all branches of production, widespread urbanization, and globalization of the economy. This study was been conducted to collect and analyze the Koryoins (Koryo saram) traditional ethnobotanical knowledge, living in Uzbekistan, whose number 174,200 people. They are the descendants of Korean immigrants to the Russian Far East, who ended up in Central Asia as a result of the forced resettlement in 1937. In the processing of collected data, four main categories of uses were defined - Alimentary, Medicinal, Household/Handicraft, and Others. For quantitative data analysis, synthetic indices were used - RFC (Relative Frequency of Citation) and CI (Cultural Importance Index), which are commonly applied to assess the importance of plants. The respondents mentioned 72 plants belonging to 28 botanical families. A significant part of them was cultivar plants. The category that had the largest number of plants mentioned by the respondents was the Alimentary use category (51). According to quantitative indices rates, the most important plants are traditionally used for food. A comparison of ethnobotanical knowledge was made with the collected data of this study and Korean traditional knowledge.

• Proceedings of the Korean Nutrition Society Conference
• /
• 2003.05a
• /
• pp.264.2-264.2
• /
• 2003