• 제목/요약/키워드: Korean landrace

검색결과 528건 처리시간 0.028초

Identification of Quantitative Trait Loci (QTL) Affecting Teat Number in Pigs

  • Kim, Tae-Hun;Choi, Bong-Hwan;Yoon, Du-Hak;Park, Eung-Woo;Jeon, Jin-Tae;Han, Jae-Young;Oh, Sung-Jong;Cheong, Il-Cheong
    • Asian-Australasian Journal of Animal Sciences
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    • 제17권9호
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    • pp.1210-1213
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    • 2004
  • Quantitative trait loci (QTL) mapping can be applied to detect chromosomal locations that control economic traits in farm animals. Teat number has been considered as one of the most important factors to evaluate mothering ability of sow. Especially, teat number is more important when the number is less than the litter size. This study was conducted to identify QTL affecting teat number in the Korean native pig${\times}$Landrace resource family. A total of 240 animals was genotyped for 132 polymorphic microsatellites covering the 18 pig autosomes. Mean and standard deviation of teat number in $F_2$animals is 13.46${\pm}$1.40. QTL was analyzed using F2 QTL Analysis Servlet of QTL express. A QTL for teat number on SSC9 was significant at the 1% chromosome-wide level and three suggestive QTL were detected on SSC3, 7 and 14. All QTL detected in this study had additive effect and Landrace alleles were associated with higher teat number in comparison with Korean native pig for three of four QTL.

Analysis of allele-specific expression using RNA-seq of the Korean native pig and Landrace reciprocal cross

  • Ahn, Byeongyong;Choi, Min-Kyeung;Yum, Joori;Cho, In-Cheol;Kim, Jin-Hoi;Park, Chankyu
    • Asian-Australasian Journal of Animal Sciences
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    • 제32권12호
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    • pp.1816-1825
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    • 2019
  • Objective: We tried to analyze allele-specific expression in the pig neocortex using bioinformatic analysis of high-throughput sequencing results from the parental genomes and offspring transcriptomes from reciprocal crosses between Korean Native and Landrace pigs. Methods: We carried out sequencing of parental genomes and offspring transcriptomes using next generation sequencing. We subsequently carried out genome scale identification of single nucleotide polymorphisms (SNPs) in two different ways using either individual genome mapping or joint genome mapping of the same breed parents that were used for the reciprocal crosses. Using parent-specific SNPs, allele-specifically expressed genes were analyzed. Results: Because of the low genome coverage (${\sim}4{\times}$) of the sequencing results, most SNPs were non-informative for parental lineage determination of the expressed alleles in the offspring and were thus excluded from our analysis. Consequently, 436 SNPs covering 336 genes were applicable to measure the imbalanced expression of paternal alleles in the offspring. By calculating the read ratios of parental alleles in the offspring, we identified seven genes showing allele-biased expression (p<0.05) including three previously reported and four newly identified genes in this study. Conclusion: The newly identified allele-specifically expressing genes in the neocortex of pigs should contribute to improving our knowledge on genomic imprinting in pigs. To our knowledge, this is the first study of allelic imbalance using high throughput analysis of both parental genomes and offspring transcriptomes of the reciprocal cross in outbred animals. Our study also showed the effect of the number of informative animals on the genome level investigation of allele-specific expression using RNA-seq analysis in livestock species.

Association of a missense mutation in the positional candidate gene glutamate receptor-interacting protein 1 with backfat thickness traits in pigs

  • Lee, Jae-Bong;Park, Hee-Bok;Yoo, Chae-Kyoung;Kim, Hee-Sung;Cho, In-Cheol;Lim, Hyun-Tae
    • Asian-Australasian Journal of Animal Sciences
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    • 제30권8호
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    • pp.1081-1085
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    • 2017
  • Objective: Previously, we reported quantitative trait loci (QTLs) affecting backfat thickness (BFT) traits on pig chromosome 5 (SW1482-SW963) in an F2 intercross population between Landrace and Korean native pigs. The aim of this study was to evaluate glutamate receptor-interacting protein 1 (GRIP1) as a positional candidate gene underlying the QTL affecting BFT traits. Methods: Genotype and phenotype analyses were performed using the 1,105 $F_2$ progeny. A mixed-effect linear model was used to access association between these single nucleotide polymorphism (SNP) markers and the BFT traits in the $F_2$ intercross population. Results: Highly significant associations of two informative SNPs (c.2442 T>C, c.3316 C>G [R1106G]) in GRIP1 with BFT traits were detected. In addition, the two SNPs were used to construct haplotypes that were also highly associated with the BFT traits. Conclusion: The SNPs and haplotypes of the GRIP1 gene determined in this study can contribute to understand the genetic structure of BFT traits in pigs.

Comparison of Live Performance and Meat Quality Parameter of Cross Bred (Korean Native Black Pig and Landrace) Pigs with Different Coat Colors

  • Hur, S.J.;Jeong, T.C.;Kim, G.D.;Jeong, J.Y.;Cho, I.C.;Lim, H.T.;Kim, B.W.;Joo, S.T.
    • Asian-Australasian Journal of Animal Sciences
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    • 제26권7호
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    • pp.1047-1053
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    • 2013
  • Five hundred and forty crossbred (Korean native black pig${\times}$Landrace) F2 were selected at a commercial pig farm and then divided into six different coat color groups: (A: Black, B: White, C: Red, D: White spot in black, E: Black spot in white, F: Black spot in red). Birth weight, 21st d weight, 140th d weight and carcass weight varied among the different coat color groups. D group (white spot in black coat) showed a significantly higher body weight at each weigh (birth weight, 140th d weight and carcass weight) than did the other groups, whereas the C group (red coat color) showed a significantly lower body weight at finishing stage (140th d weight and carcass weight) compared to other groups. Meat quality characteristics, shear force, cooking loss and meat color were not significantly different among the different coat color groups, whereas drip loss was significantly higher in F than in other groups. Most blood characteristics were not significantly different among the different groups, except for the red blood cells.

수유행동시 모돈(랜드레이스×요크셔) 발성음의 개체 판별을 위한 음성 파라미터 (Sound parameters for classifying individual sows(Landrace×Yorkshire) during nursing behavior)

  • 전중환;장홍희;하정기;김현희;구자민;이효종;연성찬
    • 대한수의학회지
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    • 제43권1호
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    • pp.165-169
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    • 2003
  • The aim of the present study was to analyse grunts of the sows and to extract parameters from the time and frequency signals in nursing behavior. Five crossbred $Landrace{\times}Yorkshire$ sows were used on day 5 or 6 postpartum. The grunts and the behaviors of the five sows were recorded with five digital camcorders. Three parameter groups [Group I: Formant vector alone, Group II: Formant vector+parameters from time signal, Group III: Formant vector+parameters from time signal-parameters eliminated by stepwise discriminant analysis backward (SDAB)] with parameter vectors extracted from single grunts in the maximum grunting rate period were used for individuality of the sows. The parameter groups were compared by a discriminant function analysis. The classification system adopted in the Group II represented the higher discriniation rate than those in other groups (Group I: 63.3%, Group II: 83.0%, Group III: 80.0%). This study demonstrated that formant, intensity, and pitch were available sound parameters for individuality of the sows during nursing behavior.

Variation and stability of agricultural characteristics in soybean landraces and cultivars

  • Seo, Min Jung;Park, Myoung Ryou;Yun, Hong Tae;Park, Chang Hwan
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.221-221
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    • 2017
  • This experiment was performed to analyze variation of growth traits of soybean to determine the information of less sensitive traits under different environmental conditions. Sowing was carried out on June 2 each year and the experimental plot was laid out in three replicates by randomized complete block design with thirty soybean varieties which consisted of 19 cultivars for beancurd and soypaste, 8 cultivars for soy-sprout including 1 landrace, and 3 cultivars for cooking with rice including 1 landrace during the period 2014-2015. The weather conditions during the experimental period were quite different with extremely low precipitation and longer sunshine duration in 2015 than 2014. The variation of characteristics related to growth period such as days of growth, days of maturity, days of flowering and the 100-seed weight was less in spite of different environmental conditions. While the variation of the number of pods per plant was high. Considering growth and seed characteristics like the number of pods per plant, the number of seeds per pod and 100-seed weight which are linked directly to the yield, cultivars such as Shingi, Daewonkong, Danbaegkong, and Daepung for beancurd and soypaste, Pungwon, Haepum and Shingang for soy-sprout and Seoritae for cooking with rice were more stable and could be expected to have high yield in Suwon, the south-central part of South Korea. These results could be useful for the selection of breeding resources to develop cultivars with high stability under changeable weather condition.

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국내 모돈에 대한 발굽 병변 조사 (Survey on claw lesions of sows in Korea)

  • 김빈;정종화;정현규;한정희
    • 한국동물위생학회지
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    • 제42권4호
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    • pp.183-191
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    • 2019
  • Claw lesion is one of the major causes of lameness in sows and the lameness is one of the reasons for culling of sows from the swine herd. This survey was conducted on sows in total of 8 farms with a total of 684 pigs. The lesions observed in the study were horizontal and vertical claw cracks (CWH & CWV), differences in the length of toes (T) and/or in the length of dew claws (DC) according to crates, breeds and parity of sows. Scores of claw lesions were assigned on 0, 1, 2, and 3 at each foot depending on the severity of the claw lesions. The sows' parity was classified into 1 to 2, 3 to 4, 5 to 8. Prevalence rate of claw lesions in sows raised on punched plastic crate was higher than that in sows raised on tribar crate. In prevlence rate by breeds, inbreed Landrace sows showed higher than that of Yorkshire sows. According to 4 claw lesions, the length of DC was the highest at parity 3~4 and CWV was the lowest at parity 3~4 in F1 crossbred sows raised on punched plastic crates, respectively. In Yorkshire breeds raised on punched plastic crates, the length of DC at parity 5~8 was the lowest and CWH was the highest at parity 3~4, respectively. In Landrace breeds raised on tribar crates, CWH was the highest at parity 1~2 and CWV was the lowest at parity 3~4, respectively. The high parity in F1 crossbred sows showed high prevalence rate of claw lesions than that of low parity and other inbreed sows, respectively. These results may be useful as elementary data in establishment of welfare quality protocol and preventive measures to reduce economic losses from craw lesions in sows.

Association of the Single Nucleotide Polymorphisms in RUNX1, DYRK1A, and KCNJ15 with Blood Related Traits in Pigs

  • Lee, Jae-Bong;Yoo, Chae-Kyoung;Park, Hee-Bok;Cho, In-Cheol;Lim, Hyun-Tae
    • Asian-Australasian Journal of Animal Sciences
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    • 제29권12호
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    • pp.1675-1681
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    • 2016
  • The aim of this study was to detect positional candidate genes located within the support interval (SI) regions based on the results of red blood cell, mean corpuscular volume (MCV), and mean corpuscular hemoglobin quantitative trait locus (QTL) in Sus scrofa chromosome 13, and to verify the correlation between specific single-nucleotide polymorphisms (SNPs) located in the exonic region of the positional candidate gene and the three genetic traits. The flanking markers of the three QTL SI regions are SW38 and S0215. Within the QTL SI regions, 44 genes were located, and runt-related transcription factor 1, dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A (DYRK1A), and potassium inwardly-rectifying channel, subfamily J, member 15 KCNJ15-which are reported to be related to the hematological traits and clinical features of Down syndrome-were selected as positional candidate genes. The ten SNPs located in the exonic region of the three genes were detected by next generation sequencing. A total of 1,232 pigs of an $F_2$ resource population between Landrace and Korean native pigs were genotyped. To investigate the effects of the three genes on each genotype, a mixed-effect model which is the considering family structure model was used to evaluate the associations between the SNPs and three genetic traits in the $F_2$ intercross population. Among them, the MCV level was highly significant (nominal $p=9.8{\times}10^{-9}$) in association with the DYRK1A-SNP1 (c.2989 G$F_2$ intercross, our approach has limited power to distinguish one particular positional candidate gene from a QTL region.

Comparison of growth performance and related gene expression of muscle and fat from Landrace, Yorkshire, and Duroc and Woori black pigs

  • Bosung Kim;Yejin Min;Yongdae Jeong;Sivasubramanian Ramani;Hyewon Lim;Yeonsu Jo;Woosang Kim;Yohan Choi;Sungkwon Park
    • Journal of Animal Science and Technology
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    • 제65권1호
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    • pp.160-174
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    • 2023
  • The purpose of this study was to compare marbling score, meat quality, juiciness, sarcomere length, and skeletal muscle satellite cell (SMSC) growth and related gene expression between Woori black pig (WB) and the Landrace, Yorkshire, and Duroc (LYD) crossbreed at different body weights (b.w.). WB was developed to improve meat quality and growth efficiency by crossbreeding Duroc with Korean native black pig. A total of 24 pigs were sacrificed when their b.w. reached about 50, 75, 100, and 120 kg. SMSC were isolated from the femoris muscles, and muscle and adipose tissues were sampled from the middle and the subcutaneous part of the femoris of hind legs, respectively. Expression levels of genes including Myoblast determination protein 1 (MyoD), Paired box gene 3 (Pax3), Myosin heavy chain (MyHC), and Myogenin, which are responsible for the growth and development of SMSC, were higher in LYD than the WB. Muscle growth inhibitor myostatin (MSTN), however, was expressed more in WB compared to LYD (p < 0.01). Numbers of SMSC extracted from femoris muscle of LYD at 50, 75, 100, and 120 kg b.w. were 8.5 ± 0.223, 8.6 ± 0.245, 7.2 ± 0.249, and 10.9 ± 0.795, and those from WB were 6.2 ± 0.32, 6.2 ± 0.374, 5.3 ± 0.423, and 17.1 ± 0.315, respectively. Expression of adipogenic genes in adipose tissue including CCAAT/enhancer-binding protein (CEBP)-β, peroxisome proliferator activated receptor (PPAR)-γ, and fatty acid synthase (FASN), were greater in WB when compared with LYD (p < 0.01). Results from the current study suggest that different muscle cell numbers between 2 different breeds might be affected by related gene expression and this warrants further investigation on other growth factors regulating animal growth and development.

우리흑돈 종모돈이 삼원교잡종 돼지의 성장, 체형 및 부분육 생산수율에 미치는 영향 (Effects of Woori Black Pig Sire on Growth Performance, Body Shape, and Retail Cut Yield of Crossbred Pigs)

  • 최요한;민예진;정현정;정용대;김정아;조은석;김영신;홍준기
    • 한국산학기술학회논문지
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    • 제21권9호
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    • pp.432-439
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    • 2020
  • 우리흑돈(Woori black pig, W)은 재래흑돼지와 두록(Duroc, D)을 이용하여 재래흑돼지의 낮은 경제적 특성을 개선시키기 위해 국립축산과학원에서 개발한 신품종이다. 따라서 본 연구는 교배 종료종모돈으로써 우리흑돈이 삼원교잡종의 성장, 체형 및 부분육 생산수율에 미치는 영향을 평가하기 위해 수행되었다. 총 32두의 삼원교잡종을 교배 종료 종모돈에 기반하여 2처리구로 완전임의 배치하였다. 교배 종료종모돈으로 두록과 우리흑돈에 기반한 2개의 다른 삼원교잡종은 LYD[(Landrace×Yorkshire)×Duroc]와 LYW[(Landrace×Yorkshire)×Woori black pig]이였다. 본 연구는 시험 개시 후 53일 동안 수행되었다. 우리흑돈과 두록 종모돈은 삼원교잡종의 53일 체중, 총증체량, 일당증체량 및 90 kg 체중 도달일령에 영향을 미치지 않았다. 체장, 체고 및 흉심에 차이가 발견되지 않았으나, 등지방두께에서 LYD(17.29 mm)와 LYW(18.96 mm) 간의 유의적인 차이가 있었다(p<0.05). LYW 삼원교잡종의 등심수율(13.11%)이 LYD(13.85%)에 비해 유의적으로 낮게 나타났다(p<0.05). LYW의 목심 수율(8.99%)은 LYD(8.21%)에 비해 유의적으로 높게 나타났다(p<0.05). 본 연구결과, 우리흑돈은 삼원교잡종의 사양성적, 체형 및 생산수율에 부정적인 영향을 미치지 않으며, 교배 종료종모돈으로 사용할 수 있을 것으로 사료된다.