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http://dx.doi.org/10.5713/ajas.16.0414

Association of a missense mutation in the positional candidate gene glutamate receptor-interacting protein 1 with backfat thickness traits in pigs  

Lee, Jae-Bong (Korea Zoonosis Research Institute (KoZRI), Chonbuk National University)
Park, Hee-Bok (Subtropical Livestock Research Institute, National Institute of Animal Science)
Yoo, Chae-Kyoung (Institute of Agriculture and Life Science, Gyeongsang National University)
Kim, Hee-Sung (Division of Applied Life Science (BK21 plus), Gyeongsang National University)
Cho, In-Cheol (Subtropical Livestock Research Institute, National Institute of Animal Science)
Lim, Hyun-Tae (Institute of Agriculture and Life Science, Gyeongsang National University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.30, no.8, 2017 , pp. 1081-1085 More about this Journal
Abstract
Objective: Previously, we reported quantitative trait loci (QTLs) affecting backfat thickness (BFT) traits on pig chromosome 5 (SW1482-SW963) in an F2 intercross population between Landrace and Korean native pigs. The aim of this study was to evaluate glutamate receptor-interacting protein 1 (GRIP1) as a positional candidate gene underlying the QTL affecting BFT traits. Methods: Genotype and phenotype analyses were performed using the 1,105 $F_2$ progeny. A mixed-effect linear model was used to access association between these single nucleotide polymorphism (SNP) markers and the BFT traits in the $F_2$ intercross population. Results: Highly significant associations of two informative SNPs (c.2442 T>C, c.3316 C>G [R1106G]) in GRIP1 with BFT traits were detected. In addition, the two SNPs were used to construct haplotypes that were also highly associated with the BFT traits. Conclusion: The SNPs and haplotypes of the GRIP1 gene determined in this study can contribute to understand the genetic structure of BFT traits in pigs.
Keywords
Glutamate Receptor-interacting Protein 1 (GRIP1); Backfat; Quantitative Trait Loci (QTL); Landrace; Korean Native Pigs;
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