• 제목/요약/키워드: Korean environmental standard method

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Application and Optimization of the IsoButoxycarbonyl Derivatization method to the Analysis of Trace Level Phenols in Environmental Samples (환경시료로부터 미량 페놀류의 분석을 위한 isoButoxycarbonyl 유도체화 분석방법 적용 및 최적화)

  • Kim, Hyub;Hong, Jong-Ki;Kim, Yong-Hwa;Kim, Kyoung-Rae
    • Environmental Analysis Health and Toxicology
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    • v.17 no.1
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    • pp.37-51
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    • 2002
  • Eleven phenols including two chlorophenols, eight alkylphenols and bisphenol A were derivatized with isobutylchloroformate to form their isobutoxycarbonyl derivatives. Standard phenol mixture was concentrated for the isobutoxycarbonyl (isoBOC) derivatization and analysed by gas chromatography/mass spectrometry (GC/MS). The recoveries of the derivatization method of alkylphenols, chlorophenols, and bisphenol A were calculated by gas chromatography/mass spectrometry-selected ion monitoring mode using two work-up methods for comparison; shaking and heating method. The linear detector responses were obtained in the concentration range of 5∼400 ng, with correlation coefficients varying from 0.9755∼0.9981. Recoveries of the alkylphenols, chlorophenols, and bisphenol A were determined by gas chromatography/mass spectrometry-selected ion monitoring mode using two work-up methods for comparison ; the US-EPA method and the isoBOC derivatization method, Eleven phenols in water samples were extracted with dichloromethane and then concentrated. Also, solid-phase extraction (SPE) with XAD-4 and subsequent conversion to isobutoxycarbonyl derivatives for sensitive analysis with the selected ion-monitoring (SIM) mode. The recoveries were 85.1∼109.9% and 90.3∼126.6% for the US-EPA method and the isoBOC.

Diversity and Genotypic Structure of ECOR Collection Determined by Repetitive Extragenic Palindromic PCR Genome Fingerprinting

  • HWANG KEUM-OK;JANG HYO-MI;CHO JAE-CHANG
    • Journal of Microbiology and Biotechnology
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    • v.15 no.3
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    • pp.672-677
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    • 2005
  • The standard reference collection of strains for E. coli, the ECOR collection, was analyzed by a genome-based typing method. Seventy-one ECOR strains were subjected to repetitive extragenic palindromic PCR genome fingerprinting with BOX primers (BOX-PCR). Using a similarity value of 0.8 or more after cluster analysis of BOX-PCR fingerprinting patterns to define the same genotypes, we identified 28 genotypes in the ECOR collection. Shannon's entropy-based diversity index was 3.07, and the incident-based coverage estimator indicated potentially 420 genotypes among E. coli populations. Chi-square test of goodness-of-fit showed statistically significant association between the genotypes defined by BOX-PCR fingerprinting and the groups previously defined by multi-locus enzyme electrophoresis. This study suggests that the diversification of E. coli strains in natural populations is actively ongoing, and rep-PCR fingerprinting is a convenient and reliable method to type E. coli strains for the purposes ranging from ecology to quarantine.ine.

Review on the Study trend and Practical Application of LCA (LCA 연구동향과 활용방안 사례)

  • Kim Yong-Ki;Yoon Hee Taek;Lee Jae Young;Kim Jin-Yong
    • Proceedings of the KSR Conference
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    • 2005.05a
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    • pp.1172-1179
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    • 2005
  • To assure our competition in the stream of international society, nowadays, environmental policy can lead to favorably environmental economy and society. Using a LCA method is authorized as one internationally as one kind of decision factor to accomplish the target of sustainable development and environment policies. It is quick development scientifically and intentionally fair standard of assessment method for pursuing environmental friendly in environment regulation policy. This paper deals with the study trend of practical application of LCA by reviewing of the domestic and abroad.

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Development of Rating Curves Using a Maximum Likelihood Model (최우도 모형을 이용한 수위-유량곡선식 개발)

  • Kim, Gyeong-Hoon;Park, Jun-Il;Shin, Chan-Ki
    • Journal of environmental and Sanitary engineering
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    • v.23 no.4
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    • pp.83-93
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    • 2008
  • The non-linear least squares model(NLSM) has long been the standard technique used by hydrologists for constructing rating curves. The reasons for its adaptation are vague, and its appropriateness as a method of describing discharge measurement uncertainty has not been well investigated. It is shown in this paper that the classical method of NLSM can model only a very limited class of variance heterogeneity. Furthermore, this lack of flexibility often leads to unaccounted heteroscedasticity, resulting in dubious values for the rating curve parameters and estimated discharge. By introducing a heteroscedastic maximum likelihood model(HMLM), the variance heterogeneity is treated more generally. The maximum likelihood model stabilises the variance better than the NLSM approach, and thus is a more robust and appropriate way to fit a rating curve to a set of discharge measurements.

Determination of Buprofezin Residues in Rice and Fruits Using HPLC with LC/MS Confirmation

  • Lee, Young-Deuk;Jang, Sang-Won
    • Korean Journal of Environmental Agriculture
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    • v.29 no.3
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    • pp.247-256
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    • 2010
  • A high-performance liquid chromatographic (HPLC) method was developed to determine buprofezin residues in hulled rice and fruits. The buprofezin residue was extracted with acetone and the extract was stepwise purified by liquid-liquid partition and Florisil column chromatography. For rice samples, acetonitrile/n-hexane partition was additionally employed to remove nonpolar lipids. Reversed phase HPLC using an octadecylsilyl column was successfully applied to separate buprofezin from sample co-extractives, as detected by ultraviolet absorption at 250 nm. Recovery experiment at the limit of quantitation validated that the proposed method could evidently determine the buprofezin residue at the level of 0.02 mg/kg. Mean recoveries from hulled rice, apple, pear, and persimmon samples fortified at three tenfold levels were in the range of 80.8~85.2%, 89.1~98.4%, 88.8~95.7% and 90.8~96.2%, respectively. Relative standard deviations of the analytical method were all less than 5%, irrespective of sample types. A selected-ion monitoring LC/mass spectrometry with positive electrospray ionization was also provided to sensitively confirm the suspected residue.

Systematic Review on Management of Livestock wastes for Improving Water Quality (수질개선을 위한 축산계 오염물질 관리방안에 대한 고찰)

  • Ahn, Ki Hong;Ryu, Hong Deok;Kim, Yong Seok
    • Journal of Korean Society on Water Environment
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    • v.31 no.5
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    • pp.576-582
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    • 2015
  • In recently, the Korea government is concerning on non-point source pollution management to improve water quality. The objective of this paper is to investigate the improvement measurement for management of livestock wastes. As a result, we find that the National Pollution Source Survey is necessary to establish the unified database system with the Korea Statistics(KOSAT) in order to minimize the difference between relevant data. The investigation for environmental impact of livestock manure should be supported the designation of control areas and establishment of the technical guidelines including target material, monitoring site, standard method, etc. In addition, it should be followed by appropriate nutrient recycling and proper fertilizer usage based on social consultation and cost-benefit analysis.

Comparison of Micro-Pipette Method and Hydrometer Method in Soil Particle Size Analysis (Micro-Pipette법과 Hydrometer법에 의한 토양 입경 분석의 비교)

  • Chung, Jong-Bae;Kim, Min-Kyeong;Kim, Bok-Jin;Kim, Kye-Hoon
    • Korean Journal of Soil Science and Fertilizer
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    • v.32 no.3
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    • pp.274-278
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    • 1999
  • Determination of soil texture, particularly the clay fraction, is an important measurement in most researches related with soil. In this study micro-pipette method For soil particle size analysis was compared with the standard hydrometer method. Micro-pipette method can eliminate the need for bulky laboratory equipment and long settling times associated with the standard hydrometer or pipette method. In the results of this investigation, the particle size data obtained with micro-pipette method were in good agreement with those found using standard hydrometer method. And with this method one person could run analysis much larger numbers of soil sample per day than with hydrometer method in relatively small laboratory space.

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Selection of Domestic Test Species Suitable for Korean Soil Ecological Risk Assessment (토양생태 위해성평가를 위한 국내 서식 토양독성 시험종 선별 연구)

  • Kim, Shin Woong;Kwak, Jin Il;Yoon, Jin-Yul;Jeong, Seung-Woo;An, Youn-Joo
    • Journal of Korean Society of Environmental Engineers
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    • v.36 no.5
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    • pp.359-366
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    • 2014
  • For an efficient and reasonable management scheme for protecting the soil environment, a soil ecological risk assessment (ERA) method should be developed prior to utilization, based on the contemporary uses and situations of each country. The Korean environmental policy focusing on soil protection is currently accelerating the development of the soil ecological risk assessment method. The soil ERA requires toxicological data on various trophic levels in the soil environment, and ultimately uses PNEC (Predicted No Effect Concentration), which is derived from collected toxicological data. Therefore, test species that are used to generate toxicity data are essential for conducting reliable ERA. This study aimed to select domestic test species for potential use in a reliable Korean ERA. Copper (Cu) and Nickel (Ni) were identified as target substances, with toxicity data (Cu, Ni) and standard test methods being collected to determine candidate species. The candidate species were first classified by soil trophic level, and then sorted into final domestic species. Forty out of 166 domestic species were determined as potential standard test species, whereas 17 out of 120 species were determined as potential Cu and Ni test species. Finally, this study presented potential soil test species based on the characteristics of the domestic soil environment, and established a preliminary step toward developing a reliable Korean soil ERA method.

A Study on the Optimal Analytical Method for the Determination of Urinary Arsenic by Hydride Generation-Atomic Absorption Spectrometry (HG-AAS법에 의한 요중 비소의 최적 분석법에 관한 연구)

  • Lee, Jong-Wha;Lee, Ui-Seon;Hong, Sung-Chul;Jang, Bong-Ki
    • Journal of Environmental Health Sciences
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    • v.35 no.5
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    • pp.402-410
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    • 2009
  • This study was carried out to examine the optimal analytical method for determination of urinary toxic arsenic (inorganic arsenic and its metabolites) by HG-AAS (hydride generation-atomic absorption spectrometry). In the analysis of SRMs (standard reference materials), method E (addition of 0.4% L-cysteine to pre-reductant and use 0.04M HCl as carrier acid) showed the most accurate results compared with the reference values. In the analysis of 30 urinary samples, analytical results were significantly different depend on the component of pre-reductant and the concentration of carrier acid. When the concentration of carrier acid was higher, the analytical result was lower. The recovery rates of MMA (monomethylarsonic acid) and DMA (dimethylarsenic acid) were varied by the concentration of pre-treatment acid and carrier acid and hydride generation reagents. When the concentration of carrier acid was 1.62 M (5% HCl), the recovery rates of DMA was 1%. The recovery rates of MMA and DMA in method E (=V) were 102% and 100%, respectively. The results of this study suggest that the component and concentration of pre-reductant and carrier acid must be carefully adjusted in the analysis of urinary arsenic, and method E is recommendable as the most precise analytical method for determination of urinary toxic arsenic.

Study for Residue Analysis of Fluxametamid in Agricultural Commodities

  • Kim, Ji Young;Choi, Yoon Ju;Kim, Jong Soo;Kim, Do Hoon;Do, Jung Ah;Jung, Yong Hyun;Lee, Kang Bong;Kim, Hyochin
    • Korean Journal of Environmental Agriculture
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    • v.38 no.1
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    • pp.1-9
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    • 2019
  • BACKGROUND: Accurate and simple analytical method determining Fluxametamid residue was necessary in various food matrices. Additionally, fulfilment of the international guideline of Codex (Codex Alimentarius Commission CAC/GL 40) was required for the analytical method. In this study, we developed Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) method to determine the Fluxametamid residue in foods. METHODS AND RESULTS: Fluxametamid was extracted with acetonitrile, partitioned and concentrated with dichloromethane. To remove the interferences, silica SPE cartridge was used before LC-MS/MS (Liquid Chromatography-Tandem Mass Spectrometry) analysis with $C_{18}$ column. Five agricultural commodities (mandarin, potato, soybean, hulled rice, and red pepper) were used as a group representative to verify the method. The liner matrix-matched calibration curves were confirmed with coefficient of determination ($r^2$) greater than 0.99 at calibration range of 0.001-0.25 mg/kg. The limits of detection and quantification were 0.001 and 0.005 mg/kg, respectively. Mean average accuracies were shown to be 82.24-115.27%. The precision was also shown to be less than 10% for all five samples. CONCLUSION: The method investigated in this study was suitable to the Codex guideline for the residue analysis. Thus, this method can be useful for determining the residue in various food matrices as routine analysis.