• 한국축산식품학회지
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• 제37권4호
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• pp.477-485
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• 2017

Novel, effective methods to control and prevent spoilage and contamination by pathogenic microorganisms in meat and meat products are in constant demand. Non-thermal pasteurization is an ideal method for the preservation of meat and meat products because it does not use heat during the pasteurization process. Atmospheric pressure cold plasma (APCP) is a new technology for the non-thermal pasteurization of meat and meat products. Several recent studies have shown that APCP treatment reduces the number of pathogenic microorganisms in meat and meat products. Furthermore, APCP treatment can be used to generate nitrite, which is an essential component of the curing process. Here, we introduce the effectiveness of APCP treatment as a pasteurization method and/or curing process for use in the meat and meat product processing industry.

• Journal of Dairy Science and Biotechnology
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• 제39권3호
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• pp.87-93
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• 2021

This study analyzed and compared growth characteristics under large-scale fermentation at 35℃ of three microorganisms with the ability to reduce odor-producing substances in livestock. The three microorganisms (Bacillus subtilis FWC1, Bacillus amyloliquefaciens NAAS1, and Pichia farinosa NAAS2) evaluated in this study have been proven effective in reducing odor-inducing substances. Bacillus subtilis FWC1 exhibited the highest viable cell count when using 2% maltodextrin as carbon source, 0.05% soy-peptone as nitrogen source, and 0.3% yeast extract. The optimum media composition for B. amyloliquefaciens NAAS1 was 1.2% modified-starch with 0.8% yeast extract. The spore formation rate in the mass production of the Bacillus strains was over 90%, indicating that optimal growth medium compositions have been identified. In the case of P. farinosa NAAS2, our optimized growth medium [2% (w/v) glucose and 1% (w/v) yeast extract] improved biomass production.

• 환경위생공학
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• 제14권1호
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• pp.24-30
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• 1999

A biological filter for treatment of toluene among volatile organic compounds was studied. The investigation was conducted using specially built stainless steel columns packed with granular activated carbon and cold for removal of toluene. The G.A. and mold as filter material was also coated with Pseudomonas putida microorganisms.The biofilter unit was operated in the condition of moisture content vairation at gas loading rate of 12.5 l/min. Gaseous toluene taken from tedlar bag was analyzed by the use of G.C equipped with F.I.d detector. The removal efficiency of gaseous toluene was 95% at average inlet concentration of 950 ppm during bio-degradation operating condition. Effective removal efficiency was obtained with moisture content 27.5% at activated carbon and 32% at mold in this study. The effective operating condition were obtained with pH 6-8, temperature 28-42℃ for microbial degradation at gas loading rate of 12.5 l/min in packed material.

• 한국환경과학회지
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• 제11권5호
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• pp.411-418
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• 2002

The current study evaluated the disinfection efficiency of free chlorine and chloramine for microorganisms on various pipe materials, such as copper, galvanized steel, carbon steel, and stainless steel. In addition, the effect of internal pipe corrosion and corrosion inhibitors on the bactericidal efficiency was evaluated using a simulated loop. For disinfection with a phosphate corrosion inhibitor, chloramination was found to be more effective than chlorination due to its persistence. Free chlorine disinfection was optimized with a high phosphoric acid concentration, while chloramine disinfection was optimized with a high phosphoric acid or low polyphosphate concentration. In simulated copper and galvanized steel loop tests, chloramination with phosphoric acid was demonstrated to be more effective.

• Journal of Microbiology and Biotechnology
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• 제19권1호
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• pp.93-98
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• 2009

The thermophilic aerobic digestion (TAD) process is recognized as an effective method for rapid waste activated sludge (WAS) degradation and the deactivation of pathogenic microorganisms. Yet, high energy costs due to heating and aeration have limited the commercialization of economical TAD processes. Previous research on autothermal thermophilic aerobic digestion (ATAD) has already reduced the heating cost. However, only a few studies have focused on reducing the aeration cost. Therefore, this study applied a two-step aeration control strategy to a fill-and-draw mode semicontinuous TAD process. The NADH-dependent fluorescence was monitored throughout the TAD experiment, and the aeration rate shifted according to the fluorescence intensity. As a result, the simple two-step aeration control operation achieved a 20.3% reduction in the total aeration, while maintaining an effective and stable operation. It is also expected that more savings can be achieved with a further reduction of the lower aeration rate or multisegmentation of the aeration rate.

• Journal of Biosystems Engineering
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• 제41권3호
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• pp.240-254
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• 2016

Background: Foodborne pathogens are a growing concern with respect to human illnesses and death. There is an increasing demand for improvements in global food safety. However, it is a challenge to detect and identify these harmful organisms in a rapid, responsive, suitable, and effective way. Results: Rapid developments in biosensor designs have contributed to the detection of foodborne pathogens and other microorganisms. Biosensors can automate this process and have the potential to enable fast analyses that are cost and time-effective. Various biosensor techniques are available that can identify foodborne pathogens and other health hazards. Conclusions: In this review, biosensor technology is briefly discussed, followed by a summary of foodborne pathogen detection using various transduction systems that exhibit specificity for particular foodborne pathogens. In addition, the recent application of biosensor technology to detect pesticides and heavy metals is briefly addressed.

• 생명과학회지
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• 제16권6호
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• pp.994-1000
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• 2006

스테비아(Stevia rebaudiana Bertoni)는 남미가 원산지인 국화과의 감미식물이다. 스테비아추출물 발효액으로부터 세균 23균주와 효모 10균주를 분리하여 일반적인 분류학적 방법과 분자유전학적 방법으로 동정하였다. 스테비아추출물 발효액에서 분리된 균주들은 5속 10종의 세균과 1종의 효모균에 속하는 것으로 나타났다.16S와 18S rDNA 염기서열 분석에 근거하여 계통수를 작성하였다. 분리균들의 항미생물 활성을 여러 세균과 식물병원성 진균들에 대해 조사하였다. 분리균들 중에서는 Lactobacillus paracasei SB13이 광범위한 세균들에 대해서 강한 항균활성을 나타내었다. 이들 결과는 토양개량을 위한 친환경적 미생물 제제를 개발하는데 도움을 줄 것이다.

• 한국임상수의학회지
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• 제37권1호
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• pp.1-8
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• 2020

Canine otitis externa is a common disorder in small animal practice with prevalence up to 20%. In a large percentage of cases, canine otitis externa is a chronic and recurrent disease also associated with drug-resistant bacteria that is difficult to treat with traditional antibiotics. Photodynamic therapy (PDT) is a new strategy to exterminate pathogenic microorganisms such as bacteria and fungi. The purpose of this study was to investigate the effectiveness of photodynamic therapy against canine otitis externa using three photosensitizer (PS); 5-Aminolevulinic acid (5-ALA) and Methyl aminolevulinic acid (MAL) with semiconductor laser diode (SLD, 635nm of wave length), Chlorophyll-lipoid complex (CLC) with light-emitting diode (LED, 660nm of wave length). After PDT, dogs showed improved Otitis Index Score (OTIS) in swelling, exudate, odor, and pain. A result of the cytology test revealed decrease of bacteria and malassezia count in the oil immersion field and colony forming units count. PDT was effective as a bacteriocide of methicillin-resistant Staphylococcus pseudintermedius (MRSP) and a fungicide of Malassezia pachydermatis. MAL and 5-ALA were more effective PS against canine otitis externa than CLC. These results suggest that PDT is a new strategy to exterminate pathogenic microorganisms such as bacteria and fungi. PDT can be considered as a new therapeutic approach for canine recurrent otitis externa and a countermeasure to drug resistance that is a disadvantage of traditional antibiotic and antifungal therapy.

• 한국식품조리과학회지
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• 제21권1호
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• pp.53-63
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• 2005

4종류의 식품 위해 세균 Salmonella typhimurium, Listeria monocytogenes, Staphylococcus aureus, Esherichia coli과 김치 부패에 관여하는 곰팡이 Aspergillus sp. Penicillium sp.에 대하여 김치 재료에 의한 항균성을 조사하였다. Paper disc method로 측정한 생육저해환 결과 4 종류의 세균에 대하여 각 재료의 methanol 추출물들은 E. coli에 대해서 재료에 따라 약간의 차이는 있으나 약 20.00 mm에 가까운 항균성을 보이는 경향을 알 수 있었으며 생강과 양파는 다른 재료와 비교해볼 때 형성된 clear zone의 크기가 작은 경향을 확인할 수 있었다. 곰팡이에 대한 결과 Aspergillus sp.과 Penicillium sp.에 대하여 마늘 추출물이 가장 우수하였고, 생강 추출물과 무 추출물도 우수한 항균 작용을 나타내었다. S. typhimurium에 대하여 농도별 생육저해는 무, 양파 추출물이 균의 중식이 활발히 일어나는 대수중식기인 4$\~$12시간 사이에 생육을 억제하는 것으로 나타났고 고춧가루 추출물도 1,000 ppm의 농도에서 S. typhimurium의 생육을 크게 억제하는 것으로 나타났으며 L. monocytogenes의 생육에 미치는 영향은 S. typhimurium에 대한 결과 유사하였으며 paper disc로 조사한 생육 저해환의 결과와 일치하는 경향을 보였다. 반면 전체적으로 김치 재료 methanol 추출물은 S. aureus 생육 저해에 큰 영향을 미치지 못했으나 특별히 1,000 ppm 농도의 마늘 추출물이 S. aureus 생육에 있어서 유도기를 8시간까지 상당히 연장시켰다. Esherichia coli의 생육에 미치는 영향을 paper disc method로 측정한 생육저해환의 결과에서 대부분의 김치 재료 methanol 추출물이 우수한 항균성을 나타낸 것과 같이 농도별 생육 곡선을 측정한 결과에서도 미약하지만 모든 추출물에서 생육 억제 효과를 나타내었다. 최소저해농도(MIC)는 고춧가루 추출물이 L. monocytogenes에 대하여 500 $\mu$g/mL로 가장 낮은 값을 보였다.

• 한국환경농학회지
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• 제12권3호
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• pp.230-238
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• 1993

폐하수중 합성세제와 중금속을 동시에 효과적으로 처리할 수 있는 다처리기능균주를 개발코자 합성세제 분해능이 우수한 P. fluorescens S1과 Pb축적균인 P. aeruginosa P1을 conjugation 시켜, 합성세제(ABS) 분해능과 Pb 축적능을 동시에 지니는 다처리기능균주 KE101, KE102 및 KE103을 개발하였다. 개발된 다처리기능균주는 Pb축적균인 P. aeruginosa P1 Plasmid가 합성세제 분해균인 P. fluorescens S1 세포내로 전달되었음을 확인하였다. 개발균주의 기질단독처리능력을 조사한 결과는 다음과 같다. 합성세제(ABS)만 단독처리했을 경우 개발균주의 합성세제(ABS) 분해능력은 합성세제 분해 원균주에 비하여 약간 감소하였으나 Pb축적 원균주의 합성세제(ABS) 분해능력에 비하여는 훨씬 증가되었다. Pb만 단독처리했을 경우 개발균주의 Pb처리능력은 Pb축적 원균주에 비하여 감소하였으나 합성세제 분해원균주의 Pb처리능력에 비하여 월등히 증가되었다. 개발균주의 기질혼합 처리능력을 조사한 결과는 다음과 같다. 합성세제(ABS)와 Pb를 혼합처리했을 경우 개발균주 KE101의 합성세제(ABS) 분해능력은 합성세제(ABS)만 단독처리했을 경우에 비하여 악 30%정도 감소되었으며, Pb처리능력은 Pb만 단독처리했을 경우와 비슷하였다. 그리고 개발균주 KE102의 합성세제(ABS)와 Pb를 혼합처리했을 경우의 합성세제(ABS) 분해능력은 합성세제(ABS)만 단독처리했을 경우에 비하여 약 20% 감소되었으나, Pb처리능력은 Pb만 단독처리했을 경우에 비하여 약 25% 증가하였다. 따라서 개발균주 KE101 및 KE102 strain은 원균주의 각 기질처리능력에 비하여는 약간 떨어겼으나 합성세제(ABS)와 중금속의 일종인 Pb를 동시에 효과적으로 처리할 수 있는 능력을 지니고 있었다.