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http://dx.doi.org/10.22424/jdsb.2021.39.3.87

Growth Media Conditions for Large-Scale Fermentation of Bacillus subtilis FWC1, B. amyloliquefaciens NAAS1, and Pichia farinosa NAAS2  

Yoo, Heeseop (JNBIO)
Yoon, Yonghee (JNBIO)
Oh, Sejong (Division of Animal Science, Chonnam National University)
Publication Information
Journal of Dairy Science and Biotechnology / v.39, no.3, 2021 , pp. 87-93 More about this Journal
Abstract
This study analyzed and compared growth characteristics under large-scale fermentation at 35℃ of three microorganisms with the ability to reduce odor-producing substances in livestock. The three microorganisms (Bacillus subtilis FWC1, Bacillus amyloliquefaciens NAAS1, and Pichia farinosa NAAS2) evaluated in this study have been proven effective in reducing odor-inducing substances. Bacillus subtilis FWC1 exhibited the highest viable cell count when using 2% maltodextrin as carbon source, 0.05% soy-peptone as nitrogen source, and 0.3% yeast extract. The optimum media composition for B. amyloliquefaciens NAAS1 was 1.2% modified-starch with 0.8% yeast extract. The spore formation rate in the mass production of the Bacillus strains was over 90%, indicating that optimal growth medium compositions have been identified. In the case of P. farinosa NAAS2, our optimized growth medium [2% (w/v) glucose and 1% (w/v) yeast extract] improved biomass production.
Keywords
optimum medium; Bacillus subtilis; Bacillus amyloliquefaciens; Pichia farinosa;
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